New rapid test for paratyphoid a fever: usefulness, cross-detection, and solution
Abstract We described a 5-min colorimetric test for paratyphoid A fever, which detects anti- Salmonella O2 antibodies by inhibiting the binding between 2 types of reagent particles. This test (TUBEX-PA) is based on that (TUBEX-TF) used for typhoid fever, which detects anti-O9 antibodies. TUBEX-PA sh...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2008-10, Vol.62 (2), p.142-150 |
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| creator | Tam, Frankie C.H Wang, Mingliu Dong, Baiqing Leung, Danny T.M Ma, Chun Hung Lim, Pak Leong |
| description | Abstract We described a 5-min colorimetric test for paratyphoid A fever, which detects anti- Salmonella O2 antibodies by inhibiting the binding between 2 types of reagent particles. This test (TUBEX-PA) is based on that (TUBEX-TF) used for typhoid fever, which detects anti-O9 antibodies. TUBEX-PA showed a sensitivity of 81.0% (47/58 culture-confirmed patients) to 93.3% (14/15) and was 98.1% (52/53) specific for healthy subjects. However, TUBEX-PA also detected 50% (7/14) to 81.8% (9/11) of typhoid patients, and conversely, TUBEX-TF detected 46.7% (7/15) to 73.3% (11/15) of paratyphoid A cases. This cross-detection could be abrogated in both tests by adding a blocker (heterologous antigen) to remove the antibodies responsible, which presumably bind to a common antigen (O12) located close to O2 and O9. The presence of anti-O12 antibodies in typhoid (9/12 or 75.0% sensitive) and paratyphoid A (22/33 or 66.7%) patients was demonstrated directly using a prototypic TUBEX test designed specifically to detect these antibodies. Thus, using TUBEX-PA and TUBEX-TF together can increase the diagnostic accuracy of detecting both typhoid and paratyphoid A fever, while the further use of differential tests allows possible immediate discrimination between these diseases. |
| doi_str_mv | 10.1016/j.diagmicrobio.2008.07.002 |
| format | Article |
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This test (TUBEX-PA) is based on that (TUBEX-TF) used for typhoid fever, which detects anti-O9 antibodies. TUBEX-PA showed a sensitivity of 81.0% (47/58 culture-confirmed patients) to 93.3% (14/15) and was 98.1% (52/53) specific for healthy subjects. However, TUBEX-PA also detected 50% (7/14) to 81.8% (9/11) of typhoid patients, and conversely, TUBEX-TF detected 46.7% (7/15) to 73.3% (11/15) of paratyphoid A cases. This cross-detection could be abrogated in both tests by adding a blocker (heterologous antigen) to remove the antibodies responsible, which presumably bind to a common antigen (O12) located close to O2 and O9. The presence of anti-O12 antibodies in typhoid (9/12 or 75.0% sensitive) and paratyphoid A (22/33 or 66.7%) patients was demonstrated directly using a prototypic TUBEX test designed specifically to detect these antibodies. 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Psychology ; Humans ; Immunoassay ; Immunoassays ; Infectious Disease ; Infectious diseases ; Internal Medicine ; Medical sciences ; Microbiology ; Middle Aged ; Miscellaneous ; O Antigens - immunology ; Paratyphoid Fever - diagnosis ; Paratyphoid Fever - microbiology ; Rapid diagnostics ; Reagent Kits, Diagnostic ; Salmonella ; Salmonella paratyphi A - classification ; Salmonella paratyphi A - immunology ; Serotyping ; Time Factors ; Typhoid</subject><ispartof>Diagnostic microbiology and infectious disease, 2008-10, Vol.62 (2), p.142-150</ispartof><rights>Elsevier Inc.</rights><rights>2008 Elsevier Inc.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-ca380088c2562e49b47d327f46c841cd7bf19229d38b21a8d021cc9aad10203e3</citedby><cites>FETCH-LOGICAL-c494t-ca380088c2562e49b47d327f46c841cd7bf19229d38b21a8d021cc9aad10203e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S073288930800309X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20718851$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18715736$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tam, Frankie C.H</creatorcontrib><creatorcontrib>Wang, Mingliu</creatorcontrib><creatorcontrib>Dong, Baiqing</creatorcontrib><creatorcontrib>Leung, Danny T.M</creatorcontrib><creatorcontrib>Ma, Chun Hung</creatorcontrib><creatorcontrib>Lim, Pak Leong</creatorcontrib><title>New rapid test for paratyphoid a fever: usefulness, cross-detection, and solution</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Abstract We described a 5-min colorimetric test for paratyphoid A fever, which detects anti- Salmonella O2 antibodies by inhibiting the binding between 2 types of reagent particles. This test (TUBEX-PA) is based on that (TUBEX-TF) used for typhoid fever, which detects anti-O9 antibodies. TUBEX-PA showed a sensitivity of 81.0% (47/58 culture-confirmed patients) to 93.3% (14/15) and was 98.1% (52/53) specific for healthy subjects. However, TUBEX-PA also detected 50% (7/14) to 81.8% (9/11) of typhoid patients, and conversely, TUBEX-TF detected 46.7% (7/15) to 73.3% (11/15) of paratyphoid A cases. This cross-detection could be abrogated in both tests by adding a blocker (heterologous antigen) to remove the antibodies responsible, which presumably bind to a common antigen (O12) located close to O2 and O9. The presence of anti-O12 antibodies in typhoid (9/12 or 75.0% sensitive) and paratyphoid A (22/33 or 66.7%) patients was demonstrated directly using a prototypic TUBEX test designed specifically to detect these antibodies. Thus, using TUBEX-PA and TUBEX-TF together can increase the diagnostic accuracy of detecting both typhoid and paratyphoid A fever, while the further use of differential tests allows possible immediate discrimination between these diseases.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Antibodies</subject><subject>Antibodies, Bacterial - blood</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Colorimetry - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Immunoassays</subject><subject>Infectious Disease</subject><subject>Infectious diseases</subject><subject>Internal Medicine</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>O Antigens - immunology</subject><subject>Paratyphoid Fever - diagnosis</subject><subject>Paratyphoid Fever - microbiology</subject><subject>Rapid diagnostics</subject><subject>Reagent Kits, Diagnostic</subject><subject>Salmonella</subject><subject>Salmonella paratyphi A - classification</subject><subject>Salmonella paratyphi A - immunology</subject><subject>Serotyping</subject><subject>Time Factors</subject><subject>Typhoid</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkt9r1TAUgIMo7jr9F6QI7mmtJ0lvk-xBGHPqYCiigm8hTU41196mJu3k_vdLvReVvehTSPjOj3znEPKMQkWBNi82lfPm69bbGFofKgYgKxAVALtHVlQKVQIIuE9WIDgrpVT8iDxKaQNAmarhITnKEF0L3qzIh3f4s4hm9K6YME1FF2Ixmmim3fgt5EdTdHiD8ayYE3ZzP2BKp0UunFLpcEI7-TCcFmZwRQr9vNwekwed6RM-OZzH5PPry08Xb8vr92-uLs6vS1ureiqt4TI3Li1bNwxr1dbCcSa6urGyptaJtqOKMeW4bBk10gGj1ipjHAUGHPkxOdnnHWP4Mefe9dYni31vBgxz0o1ai5or_k-QZiesYTKDZ3vw1_8idnqMfmviTlPQi3m90X-b14t5DUJn8zn46aHK3G7R_Qk9qM7A8wNgkjV9F81gffrNMRBUyjXN3Ks9h1nejceok_U4WHQ-Zt_aBf9__by8k8b2fvC58nfcYdqEOQ55PJrqxDToj8uuLKsCeSoc1Bd-CzgqvOs</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>Tam, Frankie C.H</creator><creator>Wang, Mingliu</creator><creator>Dong, Baiqing</creator><creator>Leung, Danny T.M</creator><creator>Ma, Chun Hung</creator><creator>Lim, Pak Leong</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20081001</creationdate><title>New rapid test for paratyphoid a fever: usefulness, cross-detection, and solution</title><author>Tam, Frankie C.H ; Wang, Mingliu ; Dong, Baiqing ; Leung, Danny T.M ; Ma, Chun Hung ; Lim, Pak Leong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-ca380088c2562e49b47d327f46c841cd7bf19229d38b21a8d021cc9aad10203e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Antibodies</topic><topic>Antibodies, Bacterial - blood</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Colorimetry - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Immunoassays</topic><topic>Infectious Disease</topic><topic>Infectious diseases</topic><topic>Internal Medicine</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>O Antigens - immunology</topic><topic>Paratyphoid Fever - diagnosis</topic><topic>Paratyphoid Fever - microbiology</topic><topic>Rapid diagnostics</topic><topic>Reagent Kits, Diagnostic</topic><topic>Salmonella</topic><topic>Salmonella paratyphi A - classification</topic><topic>Salmonella paratyphi A - immunology</topic><topic>Serotyping</topic><topic>Time Factors</topic><topic>Typhoid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tam, Frankie C.H</creatorcontrib><creatorcontrib>Wang, Mingliu</creatorcontrib><creatorcontrib>Dong, Baiqing</creatorcontrib><creatorcontrib>Leung, Danny T.M</creatorcontrib><creatorcontrib>Ma, Chun Hung</creatorcontrib><creatorcontrib>Lim, Pak Leong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tam, Frankie C.H</au><au>Wang, Mingliu</au><au>Dong, Baiqing</au><au>Leung, Danny T.M</au><au>Ma, Chun Hung</au><au>Lim, Pak Leong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New rapid test for paratyphoid a fever: usefulness, cross-detection, and solution</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2008-10-01</date><risdate>2008</risdate><volume>62</volume><issue>2</issue><spage>142</spage><epage>150</epage><pages>142-150</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>Abstract We described a 5-min colorimetric test for paratyphoid A fever, which detects anti- Salmonella O2 antibodies by inhibiting the binding between 2 types of reagent particles. This test (TUBEX-PA) is based on that (TUBEX-TF) used for typhoid fever, which detects anti-O9 antibodies. TUBEX-PA showed a sensitivity of 81.0% (47/58 culture-confirmed patients) to 93.3% (14/15) and was 98.1% (52/53) specific for healthy subjects. However, TUBEX-PA also detected 50% (7/14) to 81.8% (9/11) of typhoid patients, and conversely, TUBEX-TF detected 46.7% (7/15) to 73.3% (11/15) of paratyphoid A cases. This cross-detection could be abrogated in both tests by adding a blocker (heterologous antigen) to remove the antibodies responsible, which presumably bind to a common antigen (O12) located close to O2 and O9. The presence of anti-O12 antibodies in typhoid (9/12 or 75.0% sensitive) and paratyphoid A (22/33 or 66.7%) patients was demonstrated directly using a prototypic TUBEX test designed specifically to detect these antibodies. Thus, using TUBEX-PA and TUBEX-TF together can increase the diagnostic accuracy of detecting both typhoid and paratyphoid A fever, while the further use of differential tests allows possible immediate discrimination between these diseases.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>18715736</pmid><doi>10.1016/j.diagmicrobio.2008.07.002</doi><tpages>9</tpages></addata></record> |
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| ispartof | Diagnostic microbiology and infectious disease, 2008-10, Vol.62 (2), p.142-150 |
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| subjects | Adolescent Adult Antibodies Antibodies, Bacterial - blood Bacteriology Biological and medical sciences Child Child, Preschool Colorimetry - methods Fundamental and applied biological sciences. Psychology Humans Immunoassay Immunoassays Infectious Disease Infectious diseases Internal Medicine Medical sciences Microbiology Middle Aged Miscellaneous O Antigens - immunology Paratyphoid Fever - diagnosis Paratyphoid Fever - microbiology Rapid diagnostics Reagent Kits, Diagnostic Salmonella Salmonella paratyphi A - classification Salmonella paratyphi A - immunology Serotyping Time Factors Typhoid |
| title | New rapid test for paratyphoid a fever: usefulness, cross-detection, and solution |
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