Immunologic and histologic observations in reovirus-induced otitis media in the mouse
The goals of this study were to develop a mouse model for virally induced otitis media, and to study the immune response to infection. Intranasal inoculation of mice by reovirus was used to induce otitis media. Immunohistochemical evidence for the presence of reovirus in the nasopharynx, eustachian...
Gespeichert in:
| Veröffentlicht in: | Annals of otology, rhinology & laryngology rhinology & laryngology, 1999, Vol.108 (1), p.31-38 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 38 |
|---|---|
| container_issue | 1 |
| container_start_page | 31 |
| container_title | Annals of otology, rhinology & laryngology |
| container_volume | 108 |
| creator | MEEK, R. B BERREBI, A. S MCGREW, B. M SPIROU, G. A CUFF, C. F WETMORE, S. J |
| description | The goals of this study were to develop a mouse model for virally induced otitis media, and to study the immune response to infection. Intranasal inoculation of mice by reovirus was used to induce otitis media. Immunohistochemical evidence for the presence of reovirus in the nasopharynx, eustachian tubes, and middle ears and the amount of infiltrating B-cells and T-cells in those sites were serially evaluated by painlessly sacrificing animals over a 21 -day period. Reovirus antigen was detected in the middle ear mucosa by day 4 in 75% of infected animals, and histologic evidence for otitis media was found in 54% of all infected animals. A significant increase in B-cells in the nasopharynx and eustachian tubes was noted 7 to 10 days following infection. The number of infiltrating T-cells did not vary significantly from that in the control animals at any of the sites. These results provide a basis for further investigations of the immune response in otitis media. |
| doi_str_mv | 10.1177/000348949910800105 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_69573487</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>38241204</sourcerecordid><originalsourceid>FETCH-LOGICAL-p262t-487a2d965bb11e4d2943b756b6fdfab81ce792614e98363fd3be29c2565793fa3</originalsourceid><addsrcrecordid>eNpdkE1LxDAYhIMo67r6BwShiHir5qNJmqMsfiwseHHBW0ma1I20yZqkC_57s1g8eHoZ5mGYdwC4RPAOIc7vIYSkqkUlBII1hAjSIzBHoiIl5fj9GMwPQHkgTsFZjJ9ZVhTiGZgJQSAl9RxsVsMwOt_7D9sW0ulia2OapFfRhL1M1rtYWFcE4_c2jLG0To-t0YVPNtlYDEZbeQDS1hSDH6M5Byed7KO5mO4CbJ4e35Yv5fr1ebV8WJc7zHDKzbjEWjCqFEKm0jhXV5wyxTrdSVWj1nCBGaqMqAkjnSbKYNFiyigXpJNkAW5_c3fBf40mpmawsTV9L53JPRomKM8L8Qxe_wM__Rhc7tZgxGtBCccZupqgUeWfml2wgwzfzTRW9m8mX8ZW9l2QrrXxD0OMMpGTfgBnIXj8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>217895372</pqid></control><display><type>article</type><title>Immunologic and histologic observations in reovirus-induced otitis media in the mouse</title><source>MEDLINE</source><source>SAGE Complete A-Z List</source><creator>MEEK, R. B ; BERREBI, A. S ; MCGREW, B. M ; SPIROU, G. A ; CUFF, C. F ; WETMORE, S. J</creator><creatorcontrib>MEEK, R. B ; BERREBI, A. S ; MCGREW, B. M ; SPIROU, G. A ; CUFF, C. F ; WETMORE, S. J</creatorcontrib><description>The goals of this study were to develop a mouse model for virally induced otitis media, and to study the immune response to infection. Intranasal inoculation of mice by reovirus was used to induce otitis media. Immunohistochemical evidence for the presence of reovirus in the nasopharynx, eustachian tubes, and middle ears and the amount of infiltrating B-cells and T-cells in those sites were serially evaluated by painlessly sacrificing animals over a 21 -day period. Reovirus antigen was detected in the middle ear mucosa by day 4 in 75% of infected animals, and histologic evidence for otitis media was found in 54% of all infected animals. A significant increase in B-cells in the nasopharynx and eustachian tubes was noted 7 to 10 days following infection. The number of infiltrating T-cells did not vary significantly from that in the control animals at any of the sites. These results provide a basis for further investigations of the immune response in otitis media.</description><identifier>ISSN: 0003-4894</identifier><identifier>EISSN: 1943-572X</identifier><identifier>DOI: 10.1177/000348949910800105</identifier><identifier>PMID: 9930538</identifier><identifier>CODEN: AORHA2</identifier><language>eng</language><publisher>St. Louis, MO: Annals Publishing Compagny</publisher><subject>Animals ; Antibodies, Viral - immunology ; Antigens, Viral - immunology ; B-Lymphocytes - immunology ; Biological and medical sciences ; Disease Models, Animal ; Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology ; Ear, Middle - immunology ; Ear, Middle - pathology ; Eustachian Tube - immunology ; Eustachian Tube - pathology ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Nasopharynx - immunology ; Nasopharynx - pathology ; Non tumoral diseases ; Otitis Media - etiology ; Otitis Media - immunology ; Otorhinolaryngology. Stomatology ; Reoviridae Infections - complications ; Reoviridae Infections - immunology ; T-Lymphocytes - immunology</subject><ispartof>Annals of otology, rhinology & laryngology, 1999, Vol.108 (1), p.31-38</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright Annals Publishing Company Jan 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1656995$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9930538$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MEEK, R. B</creatorcontrib><creatorcontrib>BERREBI, A. S</creatorcontrib><creatorcontrib>MCGREW, B. M</creatorcontrib><creatorcontrib>SPIROU, G. A</creatorcontrib><creatorcontrib>CUFF, C. F</creatorcontrib><creatorcontrib>WETMORE, S. J</creatorcontrib><title>Immunologic and histologic observations in reovirus-induced otitis media in the mouse</title><title>Annals of otology, rhinology & laryngology</title><addtitle>Ann Otol Rhinol Laryngol</addtitle><description>The goals of this study were to develop a mouse model for virally induced otitis media, and to study the immune response to infection. Intranasal inoculation of mice by reovirus was used to induce otitis media. Immunohistochemical evidence for the presence of reovirus in the nasopharynx, eustachian tubes, and middle ears and the amount of infiltrating B-cells and T-cells in those sites were serially evaluated by painlessly sacrificing animals over a 21 -day period. Reovirus antigen was detected in the middle ear mucosa by day 4 in 75% of infected animals, and histologic evidence for otitis media was found in 54% of all infected animals. A significant increase in B-cells in the nasopharynx and eustachian tubes was noted 7 to 10 days following infection. The number of infiltrating T-cells did not vary significantly from that in the control animals at any of the sites. These results provide a basis for further investigations of the immune response in otitis media.</description><subject>Animals</subject><subject>Antibodies, Viral - immunology</subject><subject>Antigens, Viral - immunology</subject><subject>B-Lymphocytes - immunology</subject><subject>Biological and medical sciences</subject><subject>Disease Models, Animal</subject><subject>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</subject><subject>Ear, Middle - immunology</subject><subject>Ear, Middle - pathology</subject><subject>Eustachian Tube - immunology</subject><subject>Eustachian Tube - pathology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nasopharynx - immunology</subject><subject>Nasopharynx - pathology</subject><subject>Non tumoral diseases</subject><subject>Otitis Media - etiology</subject><subject>Otitis Media - immunology</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Reoviridae Infections - complications</subject><subject>Reoviridae Infections - immunology</subject><subject>T-Lymphocytes - immunology</subject><issn>0003-4894</issn><issn>1943-572X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkE1LxDAYhIMo67r6BwShiHir5qNJmqMsfiwseHHBW0ma1I20yZqkC_57s1g8eHoZ5mGYdwC4RPAOIc7vIYSkqkUlBII1hAjSIzBHoiIl5fj9GMwPQHkgTsFZjJ9ZVhTiGZgJQSAl9RxsVsMwOt_7D9sW0ulia2OapFfRhL1M1rtYWFcE4_c2jLG0To-t0YVPNtlYDEZbeQDS1hSDH6M5Byed7KO5mO4CbJ4e35Yv5fr1ebV8WJc7zHDKzbjEWjCqFEKm0jhXV5wyxTrdSVWj1nCBGaqMqAkjnSbKYNFiyigXpJNkAW5_c3fBf40mpmawsTV9L53JPRomKM8L8Qxe_wM__Rhc7tZgxGtBCccZupqgUeWfml2wgwzfzTRW9m8mX8ZW9l2QrrXxD0OMMpGTfgBnIXj8</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>MEEK, R. B</creator><creator>BERREBI, A. S</creator><creator>MCGREW, B. M</creator><creator>SPIROU, G. A</creator><creator>CUFF, C. F</creator><creator>WETMORE, S. J</creator><general>Annals Publishing Compagny</general><general>SAGE PUBLICATIONS, INC</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>1999</creationdate><title>Immunologic and histologic observations in reovirus-induced otitis media in the mouse</title><author>MEEK, R. B ; BERREBI, A. S ; MCGREW, B. M ; SPIROU, G. A ; CUFF, C. F ; WETMORE, S. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p262t-487a2d965bb11e4d2943b756b6fdfab81ce792614e98363fd3be29c2565793fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antibodies, Viral - immunology</topic><topic>Antigens, Viral - immunology</topic><topic>B-Lymphocytes - immunology</topic><topic>Biological and medical sciences</topic><topic>Disease Models, Animal</topic><topic>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</topic><topic>Ear, Middle - immunology</topic><topic>Ear, Middle - pathology</topic><topic>Eustachian Tube - immunology</topic><topic>Eustachian Tube - pathology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nasopharynx - immunology</topic><topic>Nasopharynx - pathology</topic><topic>Non tumoral diseases</topic><topic>Otitis Media - etiology</topic><topic>Otitis Media - immunology</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Reoviridae Infections - complications</topic><topic>Reoviridae Infections - immunology</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MEEK, R. B</creatorcontrib><creatorcontrib>BERREBI, A. S</creatorcontrib><creatorcontrib>MCGREW, B. M</creatorcontrib><creatorcontrib>SPIROU, G. A</creatorcontrib><creatorcontrib>CUFF, C. F</creatorcontrib><creatorcontrib>WETMORE, S. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Annals of otology, rhinology & laryngology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MEEK, R. B</au><au>BERREBI, A. S</au><au>MCGREW, B. M</au><au>SPIROU, G. A</au><au>CUFF, C. F</au><au>WETMORE, S. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunologic and histologic observations in reovirus-induced otitis media in the mouse</atitle><jtitle>Annals of otology, rhinology & laryngology</jtitle><addtitle>Ann Otol Rhinol Laryngol</addtitle><date>1999</date><risdate>1999</risdate><volume>108</volume><issue>1</issue><spage>31</spage><epage>38</epage><pages>31-38</pages><issn>0003-4894</issn><eissn>1943-572X</eissn><coden>AORHA2</coden><abstract>The goals of this study were to develop a mouse model for virally induced otitis media, and to study the immune response to infection. Intranasal inoculation of mice by reovirus was used to induce otitis media. Immunohistochemical evidence for the presence of reovirus in the nasopharynx, eustachian tubes, and middle ears and the amount of infiltrating B-cells and T-cells in those sites were serially evaluated by painlessly sacrificing animals over a 21 -day period. Reovirus antigen was detected in the middle ear mucosa by day 4 in 75% of infected animals, and histologic evidence for otitis media was found in 54% of all infected animals. A significant increase in B-cells in the nasopharynx and eustachian tubes was noted 7 to 10 days following infection. The number of infiltrating T-cells did not vary significantly from that in the control animals at any of the sites. These results provide a basis for further investigations of the immune response in otitis media.</abstract><cop>St. Louis, MO</cop><pub>Annals Publishing Compagny</pub><pmid>9930538</pmid><doi>10.1177/000348949910800105</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-4894 |
| ispartof | Annals of otology, rhinology & laryngology, 1999, Vol.108 (1), p.31-38 |
| issn | 0003-4894 1943-572X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69573487 |
| source | MEDLINE; SAGE Complete A-Z List |
| subjects | Animals Antibodies, Viral - immunology Antigens, Viral - immunology B-Lymphocytes - immunology Biological and medical sciences Disease Models, Animal Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology Ear, Middle - immunology Ear, Middle - pathology Eustachian Tube - immunology Eustachian Tube - pathology Medical sciences Mice Mice, Inbred BALB C Nasopharynx - immunology Nasopharynx - pathology Non tumoral diseases Otitis Media - etiology Otitis Media - immunology Otorhinolaryngology. Stomatology Reoviridae Infections - complications Reoviridae Infections - immunology T-Lymphocytes - immunology |
| title | Immunologic and histologic observations in reovirus-induced otitis media in the mouse |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T01%3A34%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immunologic%20and%20histologic%20observations%20in%20reovirus-induced%20otitis%20media%20in%20the%20mouse&rft.jtitle=Annals%20of%20otology,%20rhinology%20&%20laryngology&rft.au=MEEK,%20R.%20B&rft.date=1999&rft.volume=108&rft.issue=1&rft.spage=31&rft.epage=38&rft.pages=31-38&rft.issn=0003-4894&rft.eissn=1943-572X&rft.coden=AORHA2&rft_id=info:doi/10.1177/000348949910800105&rft_dat=%3Cproquest_pubme%3E38241204%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=217895372&rft_id=info:pmid/9930538&rfr_iscdi=true |