Proteasome inhibitors induce mitochondria-independent apoptosis in human glioma cells

The proteasome inhibitors lactacystin and AcLLNal induced p53-independent apoptosis in two human glioma cell lines, and the apoptosis was accompanied by up-regulation of immunoreactive wild-type p53, p21 Waf1, Mdm2, and p27 Kip1. Pretreatment with cycloheximide decreased the induction of cell death...

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Veröffentlicht in:	FEBS letters 1999-01, Vol.443 (2), p.181-186
Hauptverfasser:	Kitagawa, Hiroyuki, Tani, Eiichi, Ikemoto, Hideyasu, Ozaki, Isao, Nakano, Atsuhisa, Omura, Satoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	AcLLNal AcLLNal, acetyl-leucinyl-leucinyl-norleucinal Animals Apoptosis Apoptosis - drug effects Brain Neoplasms - pathology Caspase 3 Caspases - metabolism Cell Cycle Proteins Cell Line Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p27 Cyclins - metabolism Cysteine Endopeptidases - drug effects Cysteine Proteinase Inhibitors - pharmacology Cyt c, cytochrome c Cytochrome c DiOC6, 3,3′-dihexyloxacarbocyanine iodide DMEM, Dulbecco's modified Eagle's minimum essential medium DTT, dithiothreitol EDTA, ethylenediaminetetraacetic acid EGTA, ethylene-glycol-bis(β-amino-ethylether)N,N′-tetraacetic acid Enzyme Activation FCS, fetal calf serum Glioma Glioma - pathology HEPES, N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid HRP, horseradish peroxidase Humans Lactacystin mCICCP, carbonyl cyanide m-chlorophenylhydrazone Microtubule-Associated Proteins - metabolism Mitochondria - drug effects Mitochondrial membrane potential Multienzyme Complexes - drug effects NP-40, Nonidet P-40 Nuclear Proteins PARP, poly(ADP-ribose) polymerase PMA, phorbol myristate acetate PMSF, phenylmethylsulfonyl fluoride Proteasome Endopeptidase Complex Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins c-mdm2 PVDF, polyvinylidene difluoride SDS, sodium dodecylsulfate TNF, tumor necrosis factor Tumor Cells, Cultured Tumor Suppressor Protein p53 - metabolism Tumor Suppressor Proteins ΔΨ m, mitochondrial membrane potential
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