A simple pressure microinjecting system for delivery of small substance volumes to the brain: application to the developmental study of thalamo-cortical projections in foetal and neonatal rats
We describe a reliable and inexpensive method for placing injections of anatomical tracers into the brain of lower mammals. The pressure microinjecting system we developed is specifically designed to deliver very small amount of substances. The injecting portion of the system is relatively easy to a...
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| Veröffentlicht in: | Brain research. Brain research protocols 1999, Vol.3 (3), p.257-263 |
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| container_title | Brain research. Brain research protocols |
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| creator | Sbriccoli, Alessandro Carretta, Donatella Santarelli, Marialaura Pinto, Francesco Granato, Alberto Minciacchi, Diego |
| description | We describe a reliable and inexpensive method for placing injections of anatomical tracers into the brain of lower mammals. The pressure microinjecting system we developed is specifically designed to deliver very small amount of substances. The injecting portion of the system is relatively easy to assemble and can be repeatedly used for multiple experimental sessions. The system has been validated with experiments of multiple fluorescent retrograde tracing. In these experiments the populations of thalamo-cortical neurons were consistently labeled by the tracers injected bilaterally and symmetrically in the cortex of foetal and neonatal rats.
Themes: Development and regeneration
Topics: Pattern formation, compartments, and boundaries |
| doi_str_mv | 10.1016/S1385-299X(98)00047-6 |
| format | Article |
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Themes: Development and regeneration
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Themes: Development and regeneration
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Brain research protocols</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sbriccoli, Alessandro</au><au>Carretta, Donatella</au><au>Santarelli, Marialaura</au><au>Pinto, Francesco</au><au>Granato, Alberto</au><au>Minciacchi, Diego</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple pressure microinjecting system for delivery of small substance volumes to the brain: application to the developmental study of thalamo-cortical projections in foetal and neonatal rats</atitle><jtitle>Brain research. Brain research protocols</jtitle><addtitle>Brain Res Brain Res Protoc</addtitle><date>1999</date><risdate>1999</risdate><volume>3</volume><issue>3</issue><spage>257</spage><epage>263</epage><pages>257-263</pages><issn>1385-299X</issn><abstract>We describe a reliable and inexpensive method for placing injections of anatomical tracers into the brain of lower mammals. The pressure microinjecting system we developed is specifically designed to deliver very small amount of substances. The injecting portion of the system is relatively easy to assemble and can be repeatedly used for multiple experimental sessions. The system has been validated with experiments of multiple fluorescent retrograde tracing. In these experiments the populations of thalamo-cortical neurons were consistently labeled by the tracers injected bilaterally and symmetrically in the cortex of foetal and neonatal rats.
Themes: Development and regeneration
Topics: Pattern formation, compartments, and boundaries</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9974140</pmid><doi>10.1016/S1385-299X(98)00047-6</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1385-299X |
| ispartof | Brain research. Brain research protocols, 1999, Vol.3 (3), p.257-263 |
| issn | 1385-299X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Amidines - administration & dosage Amidines - pharmacokinetics Animals Animals, Newborn Brain Chemistry Brain development Cerebral Cortex - embryology Cerebral Cortex - growth & development Coloring Agents - administration & dosage Coloring Agents - pharmacokinetics Female Fluorescent tracing Microinjection system Microinjections - instrumentation Neural Pathways - embryology Neural Pathways - growth & development Pregnancy Pressure Rats Rats, Wistar Thalamo-cortical connection Thalamus - embryology Thalamus - growth & development |
| title | A simple pressure microinjecting system for delivery of small substance volumes to the brain: application to the developmental study of thalamo-cortical projections in foetal and neonatal rats |
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