Interaction of the Capsid Protein p24 (HIV-1) with Sequence-Derived Peptides: Influence on p24 Dimerization
Protein–protein interactions of the p24 (HIV-1) capsid protein play an essential role in the production of infectious virus particles. To map the putative p24 dimerization site, a set of overlapping peptides spanning the p24 sequence was prepared using spot synthesis on a cellulose membrane and prob...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1999-02, Vol.254 (1), p.6-10 |
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| container_title | Virology (New York, N.Y.) |
| container_volume | 254 |
| creator | Hilpert, K. Behlke, J. Scholz, Ch Misselwitz, R. Schneider-Mergener, J. Höhne, W. |
| description | Protein–protein interactions of the p24 (HIV-1) capsid protein play an essential role in the production of infectious virus particles. To map the putative p24 dimerization site, a set of overlapping peptides spanning the p24 sequence was prepared using spot synthesis on a cellulose membrane and probed with recombinant p24 (rp24). Three sequence regions interacting with rp24 were identified. Peptides from each region were synthesized, but only one peptide was effectively able to inhibit rp24 dimerization in solution. Amino acids that were exposed in the corresponding p24 region were mutated in rp24, resulting in a significant decrease of rp24 dimerization. Thus, participation of this region in virus capsid assembly can be assumed. |
| doi_str_mv | 10.1006/viro.1998.9526 |
| format | Article |
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To map the putative p24 dimerization site, a set of overlapping peptides spanning the p24 sequence was prepared using spot synthesis on a cellulose membrane and probed with recombinant p24 (rp24). Three sequence regions interacting with rp24 were identified. Peptides from each region were synthesized, but only one peptide was effectively able to inhibit rp24 dimerization in solution. Amino acids that were exposed in the corresponding p24 region were mutated in rp24, resulting in a significant decrease of rp24 dimerization. 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| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1999-02, Vol.254 (1), p.6-10 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69569871 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | AIDS/HIV Animals Binding Sites Dimerization HIV Core Protein p24 - metabolism HIV-1 capsid protein Human immunodeficiency virus 1 Humans Mice p24 peptide scan peptide/protein interaction Peptides - metabolism spot synthesis substitutional analysis |
| title | Interaction of the Capsid Protein p24 (HIV-1) with Sequence-Derived Peptides: Influence on p24 Dimerization |
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