Fish Androgen Receptor: cDNA Cloning, Steroid Activation of Transcription in Transfected Mammalian Cells, and Tissue mRNA Levels
We have previously identified 11-ketotestosterone (11KT, a portent androgen in teleosts) as the spermatogenesis-inducing hormone of the Japanese eel. In this study, a cDNA encoding the eel androgen receptor (AR) was isolated from the Japanese eel testis. This cDNA contains a complete open reading fr...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1999-01, Vol.254 (2), p.378-383 |
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| creator | Todo, Takashi Ikeuchi, Toshitaka Kobayashi, Tohru Nagahama, Yoshitaka |
| description | We have previously identified 11-ketotestosterone (11KT, a portent androgen in teleosts) as the spermatogenesis-inducing hormone of the Japanese eel. In this study, a cDNA encoding the eel androgen receptor (AR) was isolated from the Japanese eel testis. This cDNA contains a complete open reading frame encoding 848 amino residues. The amino acid sequence of the eel AR shows high homology with other ARs. In transient transfection assays of mammalian cells, the eel AR showed androgen-dependent activation of transcription from the androgen-responsive MMTV promoter. Of the endogenous androgens found in the Japanese eel, 11KT was the most effective activator of transcription. These results indicate that the cloned cDNA encodes a functional eel AR, and its major native ligand is 11KT. This is the first isolation of an AR molecule from fish, and is the first evidence that 11KT acts via a nuclear receptor. |
| doi_str_mv | 10.1006/bbrc.1998.9919 |
| format | Article |
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In this study, a cDNA encoding the eel androgen receptor (AR) was isolated from the Japanese eel testis. This cDNA contains a complete open reading frame encoding 848 amino residues. The amino acid sequence of the eel AR shows high homology with other ARs. In transient transfection assays of mammalian cells, the eel AR showed androgen-dependent activation of transcription from the androgen-responsive MMTV promoter. Of the endogenous androgens found in the Japanese eel, 11KT was the most effective activator of transcription. These results indicate that the cloned cDNA encodes a functional eel AR, and its major native ligand is 11KT. 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In this study, a cDNA encoding the eel androgen receptor (AR) was isolated from the Japanese eel testis. This cDNA contains a complete open reading frame encoding 848 amino residues. The amino acid sequence of the eel AR shows high homology with other ARs. In transient transfection assays of mammalian cells, the eel AR showed androgen-dependent activation of transcription from the androgen-responsive MMTV promoter. Of the endogenous androgens found in the Japanese eel, 11KT was the most effective activator of transcription. These results indicate that the cloned cDNA encodes a functional eel AR, and its major native ligand is 11KT. This is the first isolation of an AR molecule from fish, and is the first evidence that 11KT acts via a nuclear receptor.</description><subject>Amino Acid Sequence</subject><subject>Androgens - pharmacology</subject><subject>Anguilla japonica</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Brackish</subject><subject>Cell Line</subject><subject>Chorionic Gonadotropin - pharmacology</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary</subject><subject>Eels</subject><subject>Freshwater</subject><subject>Humans</subject><subject>Kidney</subject><subject>Male</subject><subject>Mammals</subject><subject>Marine</subject><subject>Molecular Sequence Data</subject><subject>Organ Specificity</subject><subject>Receptors, Androgen - chemistry</subject><subject>Receptors, Androgen - genetics</subject><subject>Receptors, Androgen - physiology</subject><subject>Recombinant Proteins - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Testis - metabolism</subject><subject>Testosterone - analogs & derivatives</subject><subject>Testosterone - pharmacology</subject><subject>Transcription, Genetic - drug effects</subject><subject>Transcriptional Activation - drug effects</subject><subject>Transfection</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rGzEQhkVoSZ2k19wKOuXkdaVd7a6Um3E-wU0gdaE3IUujRGFXcqS1Ibf89Mixya0UBAMzj16GeRA6pWRCCWl-LpdRT6gQfCIEFQdoRIkgRUkJ-4JGJBNFKejfb-gopWdCKGWNOESHmeWcNSP0duXSE556E8MjePwAGlZDiOdYX9xN8awL3vnHMf49QAzO4Kke3EYNLngcLF5E5ZOObvXRcH7XsKAHMPiX6nvVOeXxDLoujbHyBi9cSmvA_UMOn8MGunSCvlrVJfi-r8foz9XlYnZTzO-vb2fTeaFZ1QxFZVrb0qqqGamprepSlFYpRvIDYo1QJdOm1qq21mqheUuVIlS1DWt5SzirjtHZLncVw8sa0iB7l3TeTHkI6yQbUTet4M1_QZrXqDmtMzjZgTqGlCJYuYquV_FVUiK3buTWjdy6kVs3-cOPffJ62YP5xPcy8pzv5vkssHEQZdIOvAbjYr6pNMH9K_odDu2d2g</recordid><startdate>19990119</startdate><enddate>19990119</enddate><creator>Todo, Takashi</creator><creator>Ikeuchi, Toshitaka</creator><creator>Kobayashi, Tohru</creator><creator>Nagahama, Yoshitaka</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990119</creationdate><title>Fish Androgen Receptor: cDNA Cloning, Steroid Activation of Transcription in Transfected Mammalian Cells, and Tissue mRNA Levels</title><author>Todo, Takashi ; Ikeuchi, Toshitaka ; Kobayashi, Tohru ; Nagahama, Yoshitaka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-3d7f713354051f35292faa40a40e0fd9a24cd5ca5fffc9c871aa01a7647870843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Androgens - pharmacology</topic><topic>Anguilla japonica</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Brackish</topic><topic>Cell Line</topic><topic>Chorionic Gonadotropin - pharmacology</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary</topic><topic>Eels</topic><topic>Freshwater</topic><topic>Humans</topic><topic>Kidney</topic><topic>Male</topic><topic>Mammals</topic><topic>Marine</topic><topic>Molecular Sequence Data</topic><topic>Organ Specificity</topic><topic>Receptors, Androgen - chemistry</topic><topic>Receptors, Androgen - genetics</topic><topic>Receptors, Androgen - physiology</topic><topic>Recombinant Proteins - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Testis - metabolism</topic><topic>Testosterone - analogs & derivatives</topic><topic>Testosterone - pharmacology</topic><topic>Transcription, Genetic - drug effects</topic><topic>Transcriptional Activation - drug effects</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Todo, Takashi</creatorcontrib><creatorcontrib>Ikeuchi, Toshitaka</creatorcontrib><creatorcontrib>Kobayashi, Tohru</creatorcontrib><creatorcontrib>Nagahama, Yoshitaka</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Todo, Takashi</au><au>Ikeuchi, Toshitaka</au><au>Kobayashi, Tohru</au><au>Nagahama, Yoshitaka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fish Androgen Receptor: cDNA Cloning, Steroid Activation of Transcription in Transfected Mammalian Cells, and Tissue mRNA Levels</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1999-01-19</date><risdate>1999</risdate><volume>254</volume><issue>2</issue><spage>378</spage><epage>383</epage><pages>378-383</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We have previously identified 11-ketotestosterone (11KT, a portent androgen in teleosts) as the spermatogenesis-inducing hormone of the Japanese eel. In this study, a cDNA encoding the eel androgen receptor (AR) was isolated from the Japanese eel testis. This cDNA contains a complete open reading frame encoding 848 amino residues. The amino acid sequence of the eel AR shows high homology with other ARs. In transient transfection assays of mammalian cells, the eel AR showed androgen-dependent activation of transcription from the androgen-responsive MMTV promoter. Of the endogenous androgens found in the Japanese eel, 11KT was the most effective activator of transcription. These results indicate that the cloned cDNA encodes a functional eel AR, and its major native ligand is 11KT. This is the first isolation of an AR molecule from fish, and is the first evidence that 11KT acts via a nuclear receptor.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9918846</pmid><doi>10.1006/bbrc.1998.9919</doi><tpages>6</tpages></addata></record> |
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| subjects | Amino Acid Sequence Androgens - pharmacology Anguilla japonica Animals Base Sequence Brackish Cell Line Chorionic Gonadotropin - pharmacology Cloning, Molecular DNA, Complementary Eels Freshwater Humans Kidney Male Mammals Marine Molecular Sequence Data Organ Specificity Receptors, Androgen - chemistry Receptors, Androgen - genetics Receptors, Androgen - physiology Recombinant Proteins - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Sequence Alignment Sequence Homology, Amino Acid Testis - metabolism Testosterone - analogs & derivatives Testosterone - pharmacology Transcription, Genetic - drug effects Transcriptional Activation - drug effects Transfection |
| title | Fish Androgen Receptor: cDNA Cloning, Steroid Activation of Transcription in Transfected Mammalian Cells, and Tissue mRNA Levels |
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