Retinal pigment epithelial cells from Royal College of Surgeons dystrophic rats can take up melanin granules
Many successful pigment epithelium transplantation studies involving pink-eyed Royal College of Surgeons (RCS) dystrophic rats showed highly pigmented transplanted cells forming a double layer with slightly pigmented cells, attached to Bruch's membrane. Since it is not clear whether transplante...
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| description | Many successful pigment epithelium transplantation studies involving pink-eyed Royal College of Surgeons (RCS) dystrophic rats showed highly pigmented transplanted cells forming a double layer with slightly pigmented cells, attached to Bruch's membrane. Since it is not clear whether transplanted pigmented cells can displace retinal pigment epithelial (RPE) host cells from Bruch's membrane, we suggested that RPE cells of RCS dystrophic rats can phagocytize melanin granules, possibly derived from perished transplanted cells.
In a series of three experiments, RPE cells of nine pink-eyed, 2 1/2-month-old RCS dystrophic rats were isolated by trypsinization and mechanical dissection and cultivated in Dulbecco's modified Eagles' medium. These cells were then fed with melanin granules, isolated from bovine RPE cells, double-trypsinized after phagocytosis and viewed by light and electron microscopy. We also transplanted iris pigment epithelial (IPE) cells of 20-day-old Long-Evans rats into the subretinal space of pink-eyed RCS dystrophic rats of the same age, shown in light-microscopic photography after 42 days.
Living RPE cells were heavily pigmented after feeding with isolated melanin granules in all three experiments as viewed by light microscopy. In addition, we identified melanin granules phagocytized by dystrophic RPE cells in electron microscopy. After transplantation of pigmented IPE cells into the subretinal space of pink-eyed RCS dystrophic rats' eyes, a layer of slightly pigmented cells was seen on Bruch's membrane below the transplanted IPE cells, shown in light microscopy.
We have shown by phagocytosis assay that dystrophic RPE cells can take up melanin granules in vitro. Our results assume that pigmented cells in transplantation studies, found as a monolayer, attached to Bruch's membrane, cannot automatically be identified as transplanted cells. Instead, the possibility of perished transplanted cells serving as melanin donors for RPE host cells must be taken into consideration. |
| doi_str_mv | 10.1007/s004170050196 |
| format | Article |
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In a series of three experiments, RPE cells of nine pink-eyed, 2 1/2-month-old RCS dystrophic rats were isolated by trypsinization and mechanical dissection and cultivated in Dulbecco's modified Eagles' medium. These cells were then fed with melanin granules, isolated from bovine RPE cells, double-trypsinized after phagocytosis and viewed by light and electron microscopy. We also transplanted iris pigment epithelial (IPE) cells of 20-day-old Long-Evans rats into the subretinal space of pink-eyed RCS dystrophic rats of the same age, shown in light-microscopic photography after 42 days.
Living RPE cells were heavily pigmented after feeding with isolated melanin granules in all three experiments as viewed by light microscopy. In addition, we identified melanin granules phagocytized by dystrophic RPE cells in electron microscopy. After transplantation of pigmented IPE cells into the subretinal space of pink-eyed RCS dystrophic rats' eyes, a layer of slightly pigmented cells was seen on Bruch's membrane below the transplanted IPE cells, shown in light microscopy.
We have shown by phagocytosis assay that dystrophic RPE cells can take up melanin granules in vitro. Our results assume that pigmented cells in transplantation studies, found as a monolayer, attached to Bruch's membrane, cannot automatically be identified as transplanted cells. Instead, the possibility of perished transplanted cells serving as melanin donors for RPE host cells must be taken into consideration.</description><identifier>ISSN: 0721-832X</identifier><identifier>EISSN: 1435-702X</identifier><identifier>DOI: 10.1007/s004170050196</identifier><identifier>PMID: 9951644</identifier><identifier>CODEN: GACODL</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Biological and medical sciences ; Cattle ; Cell Transplantation ; Cells, Cultured ; Iris - cytology ; Medical sciences ; Melanins - metabolism ; Ophthalmology ; Phagocytosis - physiology ; Pigment Epithelium of Eye - cytology ; Pigment Epithelium of Eye - physiology ; Pigment Epithelium of Eye - ultrastructure ; Rats ; Rats, Long-Evans ; Rats, Mutant Strains ; Retinal Degeneration - metabolism ; Retinal Degeneration - pathology ; Retinal Degeneration - surgery ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the eye and orbit</subject><ispartof>Graefe's archive for clinical and experimental ophthalmology, 1999, Vol.237 (1), p.67-71</ispartof><rights>1999 INIST-CNRS</rights><rights>Springer-Verlag Berlin Heidelberg 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-50df59654f69413276b8921817739b267be30c7857ca38471ddd0ca89708bcce3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1650906$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9951644$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BRAUN, M</creatorcontrib><creatorcontrib>KAGE, A</creatorcontrib><creatorcontrib>HEIMANN, K</creatorcontrib><creatorcontrib>SCHRAERMEYER, U</creatorcontrib><title>Retinal pigment epithelial cells from Royal College of Surgeons dystrophic rats can take up melanin granules</title><title>Graefe's archive for clinical and experimental ophthalmology</title><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><description>Many successful pigment epithelium transplantation studies involving pink-eyed Royal College of Surgeons (RCS) dystrophic rats showed highly pigmented transplanted cells forming a double layer with slightly pigmented cells, attached to Bruch's membrane. Since it is not clear whether transplanted pigmented cells can displace retinal pigment epithelial (RPE) host cells from Bruch's membrane, we suggested that RPE cells of RCS dystrophic rats can phagocytize melanin granules, possibly derived from perished transplanted cells.
In a series of three experiments, RPE cells of nine pink-eyed, 2 1/2-month-old RCS dystrophic rats were isolated by trypsinization and mechanical dissection and cultivated in Dulbecco's modified Eagles' medium. These cells were then fed with melanin granules, isolated from bovine RPE cells, double-trypsinized after phagocytosis and viewed by light and electron microscopy. We also transplanted iris pigment epithelial (IPE) cells of 20-day-old Long-Evans rats into the subretinal space of pink-eyed RCS dystrophic rats of the same age, shown in light-microscopic photography after 42 days.
Living RPE cells were heavily pigmented after feeding with isolated melanin granules in all three experiments as viewed by light microscopy. In addition, we identified melanin granules phagocytized by dystrophic RPE cells in electron microscopy. After transplantation of pigmented IPE cells into the subretinal space of pink-eyed RCS dystrophic rats' eyes, a layer of slightly pigmented cells was seen on Bruch's membrane below the transplanted IPE cells, shown in light microscopy.
We have shown by phagocytosis assay that dystrophic RPE cells can take up melanin granules in vitro. Our results assume that pigmented cells in transplantation studies, found as a monolayer, attached to Bruch's membrane, cannot automatically be identified as transplanted cells. Instead, the possibility of perished transplanted cells serving as melanin donors for RPE host cells must be taken into consideration.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell Transplantation</subject><subject>Cells, Cultured</subject><subject>Iris - cytology</subject><subject>Medical sciences</subject><subject>Melanins - metabolism</subject><subject>Ophthalmology</subject><subject>Phagocytosis - physiology</subject><subject>Pigment Epithelium of Eye - cytology</subject><subject>Pigment Epithelium of Eye - physiology</subject><subject>Pigment Epithelium of Eye - ultrastructure</subject><subject>Rats</subject><subject>Rats, Long-Evans</subject><subject>Rats, Mutant Strains</subject><subject>Retinal Degeneration - metabolism</subject><subject>Retinal Degeneration - pathology</subject><subject>Retinal Degeneration - surgery</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the eye and orbit</subject><issn>0721-832X</issn><issn>1435-702X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkc1r3DAUxEVJSTZpjz0WRCi9uX2yvqxjWNKmECikLeRmZPl5o0S2HMk-7H9fhSwJ7enBzI-BN0PIBwZfGID-mgEE0wASmFFvyIYJLisN9e0R2YCuWdXw-vaEnOZ8DwXlkh2TY2MkU0JsSLjBxU820NnvRpwWirNf7jD4IjkMIdMhxZHexH0RtjEE3CGNA_21ph3GKdN-n5cU5zvvaLJLps5OdLEPSNeZjhjs5Ce6S3ZaA-Z35O1gQ8b3h3tG_ny7_L29qq5_fv-xvbiuHBdiqST0gzRKikEZwXitVdeYmjVMa266WukOOTjdSO0sb4Rmfd-Ds43R0HTOIT8jn59z5xQfV8xLO_r89I2dMK65VUYqqaUp4Pl_4H1cU6kjtzUHbaTUdYGqZ8ilmHPCoZ2TH23atwzapwnafyYo_MdD6NqN2L_Qh86L_-ng2-xsGEo5zufXUCXBgOJ_Ac06jV4</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>BRAUN, M</creator><creator>KAGE, A</creator><creator>HEIMANN, K</creator><creator>SCHRAERMEYER, U</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>1999</creationdate><title>Retinal pigment epithelial cells from Royal College of Surgeons dystrophic rats can take up melanin granules</title><author>BRAUN, M ; KAGE, A ; HEIMANN, K ; SCHRAERMEYER, U</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-50df59654f69413276b8921817739b267be30c7857ca38471ddd0ca89708bcce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cell Transplantation</topic><topic>Cells, Cultured</topic><topic>Iris - cytology</topic><topic>Medical sciences</topic><topic>Melanins - metabolism</topic><topic>Ophthalmology</topic><topic>Phagocytosis - physiology</topic><topic>Pigment Epithelium of Eye - cytology</topic><topic>Pigment Epithelium of Eye - physiology</topic><topic>Pigment Epithelium of Eye - ultrastructure</topic><topic>Rats</topic><topic>Rats, Long-Evans</topic><topic>Rats, Mutant Strains</topic><topic>Retinal Degeneration - metabolism</topic><topic>Retinal Degeneration - pathology</topic><topic>Retinal Degeneration - surgery</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the eye and orbit</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BRAUN, M</creatorcontrib><creatorcontrib>KAGE, A</creatorcontrib><creatorcontrib>HEIMANN, K</creatorcontrib><creatorcontrib>SCHRAERMEYER, U</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BRAUN, M</au><au>KAGE, A</au><au>HEIMANN, K</au><au>SCHRAERMEYER, U</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Retinal pigment epithelial cells from Royal College of Surgeons dystrophic rats can take up melanin granules</atitle><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><date>1999</date><risdate>1999</risdate><volume>237</volume><issue>1</issue><spage>67</spage><epage>71</epage><pages>67-71</pages><issn>0721-832X</issn><eissn>1435-702X</eissn><coden>GACODL</coden><abstract>Many successful pigment epithelium transplantation studies involving pink-eyed Royal College of Surgeons (RCS) dystrophic rats showed highly pigmented transplanted cells forming a double layer with slightly pigmented cells, attached to Bruch's membrane. Since it is not clear whether transplanted pigmented cells can displace retinal pigment epithelial (RPE) host cells from Bruch's membrane, we suggested that RPE cells of RCS dystrophic rats can phagocytize melanin granules, possibly derived from perished transplanted cells.
In a series of three experiments, RPE cells of nine pink-eyed, 2 1/2-month-old RCS dystrophic rats were isolated by trypsinization and mechanical dissection and cultivated in Dulbecco's modified Eagles' medium. These cells were then fed with melanin granules, isolated from bovine RPE cells, double-trypsinized after phagocytosis and viewed by light and electron microscopy. We also transplanted iris pigment epithelial (IPE) cells of 20-day-old Long-Evans rats into the subretinal space of pink-eyed RCS dystrophic rats of the same age, shown in light-microscopic photography after 42 days.
Living RPE cells were heavily pigmented after feeding with isolated melanin granules in all three experiments as viewed by light microscopy. In addition, we identified melanin granules phagocytized by dystrophic RPE cells in electron microscopy. After transplantation of pigmented IPE cells into the subretinal space of pink-eyed RCS dystrophic rats' eyes, a layer of slightly pigmented cells was seen on Bruch's membrane below the transplanted IPE cells, shown in light microscopy.
We have shown by phagocytosis assay that dystrophic RPE cells can take up melanin granules in vitro. Our results assume that pigmented cells in transplantation studies, found as a monolayer, attached to Bruch's membrane, cannot automatically be identified as transplanted cells. Instead, the possibility of perished transplanted cells serving as melanin donors for RPE host cells must be taken into consideration.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>9951644</pmid><doi>10.1007/s004170050196</doi><tpages>5</tpages></addata></record> |
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| ispartof | Graefe's archive for clinical and experimental ophthalmology, 1999, Vol.237 (1), p.67-71 |
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| subjects | Animals Biological and medical sciences Cattle Cell Transplantation Cells, Cultured Iris - cytology Medical sciences Melanins - metabolism Ophthalmology Phagocytosis - physiology Pigment Epithelium of Eye - cytology Pigment Epithelium of Eye - physiology Pigment Epithelium of Eye - ultrastructure Rats Rats, Long-Evans Rats, Mutant Strains Retinal Degeneration - metabolism Retinal Degeneration - pathology Retinal Degeneration - surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the eye and orbit |
| title | Retinal pigment epithelial cells from Royal College of Surgeons dystrophic rats can take up melanin granules |
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