Absorption of calcium oxalate does not require dissociation in rats
Calcium absorption is thought to occur only if calcium is in a soluble or dissociated form, although experimental evidence is lacking. The intestinal absorption of calcium oxalate a small, neutral and virtually insoluble calcium salt, was elucidated in the whole body of awake rats. Suspensions of 45...
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| Veröffentlicht in: | The Journal of nutrition 1999, Vol.129 (1), p.170-173 |
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| creator | Hanes, D.A Weaver, C.M Heaney, R.P Wastney, M |
| description | Calcium absorption is thought to occur only if calcium is in a soluble or dissociated form, although experimental evidence is lacking. The intestinal absorption of calcium oxalate a small, neutral and virtually insoluble calcium salt, was elucidated in the whole body of awake rats. Suspensions of 45Ca ascorbate, 14C oxalic acid and doubly labeled 45Ca-[14C]-oxalate were given by gavage to separate groups of rats. Following dosing, blood samples were drawn for up to 240 min through a previously inserted intravenous catheter. Serum was assayed for radioactive tracers, and data were then plotted as fraction of dose over time. Calcium absorption was 15% [with a loading of 0.3 mmol (15 mg) calcium], oxalic acid absorption was 22% and Ca-oxalate absorption was |
| doi_str_mv | 10.1093/jn/129.1.170 |
| format | Article |
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The intestinal absorption of calcium oxalate a small, neutral and virtually insoluble calcium salt, was elucidated in the whole body of awake rats. Suspensions of 45Ca ascorbate, 14C oxalic acid and doubly labeled 45Ca-[14C]-oxalate were given by gavage to separate groups of rats. Following dosing, blood samples were drawn for up to 240 min through a previously inserted intravenous catheter. Serum was assayed for radioactive tracers, and data were then plotted as fraction of dose over time. Calcium absorption was 15% [with a loading of 0.3 mmol (15 mg) calcium], oxalic acid absorption was 22% and Ca-oxalate absorption was <2%. Appearance of 45Ca from calcium ascorbate and 14C from oxalic acid differed, whereas 45Ca and 14C from doubly labeled Ca-oxalate had identical serum appearance profiles. Therefore, we conclude that calcium oxalate was absorbed intact. Addition of excess, unlabeled calcium to the doubly-labeled calcium oxalate did not alter the relationship of the serum level of the two tracers, confirming absorption of calcium oxalate as the intact salt. Thus, calcium bound as a small, neutral, calcium salt such as calcium oxalate does not have to be dissociated prior to absorption. Possibly other small compounds would be similarly absorbed. These results alter our current understanding of calcium bioavailability from foods and therapeutic agents.</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.1093/jn/129.1.170</identifier><identifier>PMID: 9915895</identifier><identifier>CODEN: JONUAI</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Nutritional Sciences</publisher><subject>Absorption ; animal models ; Animals ; bioavailability ; Biological and medical sciences ; blood serum ; Calcium ; Calcium - pharmacokinetics ; calcium absorption ; Calcium Oxalate - pharmacokinetics ; calcium oxide ; Calcium Radioisotopes ; Carbon Radioisotopes ; Fundamental and applied biological sciences. Psychology ; intestinal absorption ; kinetics ; Laboratory animals ; Male ; Metabolisms and neurohumoral controls ; oxalic acid ; Oxalic Acid - pharmacokinetics ; Rats ; Rats, Sprague-Dawley ; Rodents ; Vertebrates: anatomy and physiology, studies on body, several organs or systems ; Water and mineral metabolism. Osmoregulation. Acidobasic balance</subject><ispartof>The Journal of nutrition, 1999, Vol.129 (1), p.170-173</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright American Institute of Nutrition Jan 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-d802dc994943a60aa061d58459b2eafe46459fedbaa7743ebc209aa2380d84b73</citedby><cites>FETCH-LOGICAL-c404t-d802dc994943a60aa061d58459b2eafe46459fedbaa7743ebc209aa2380d84b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022,27922,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1722108$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9915895$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hanes, D.A</creatorcontrib><creatorcontrib>Weaver, C.M</creatorcontrib><creatorcontrib>Heaney, R.P</creatorcontrib><creatorcontrib>Wastney, M</creatorcontrib><title>Absorption of calcium oxalate does not require dissociation in rats</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>Calcium absorption is thought to occur only if calcium is in a soluble or dissociated form, although experimental evidence is lacking. The intestinal absorption of calcium oxalate a small, neutral and virtually insoluble calcium salt, was elucidated in the whole body of awake rats. Suspensions of 45Ca ascorbate, 14C oxalic acid and doubly labeled 45Ca-[14C]-oxalate were given by gavage to separate groups of rats. Following dosing, blood samples were drawn for up to 240 min through a previously inserted intravenous catheter. Serum was assayed for radioactive tracers, and data were then plotted as fraction of dose over time. Calcium absorption was 15% [with a loading of 0.3 mmol (15 mg) calcium], oxalic acid absorption was 22% and Ca-oxalate absorption was <2%. Appearance of 45Ca from calcium ascorbate and 14C from oxalic acid differed, whereas 45Ca and 14C from doubly labeled Ca-oxalate had identical serum appearance profiles. Therefore, we conclude that calcium oxalate was absorbed intact. Addition of excess, unlabeled calcium to the doubly-labeled calcium oxalate did not alter the relationship of the serum level of the two tracers, confirming absorption of calcium oxalate as the intact salt. Thus, calcium bound as a small, neutral, calcium salt such as calcium oxalate does not have to be dissociated prior to absorption. Possibly other small compounds would be similarly absorbed. These results alter our current understanding of calcium bioavailability from foods and therapeutic agents.</description><subject>Absorption</subject><subject>animal models</subject><subject>Animals</subject><subject>bioavailability</subject><subject>Biological and medical sciences</subject><subject>blood serum</subject><subject>Calcium</subject><subject>Calcium - pharmacokinetics</subject><subject>calcium absorption</subject><subject>Calcium Oxalate - pharmacokinetics</subject><subject>calcium oxide</subject><subject>Calcium Radioisotopes</subject><subject>Carbon Radioisotopes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>intestinal absorption</subject><subject>kinetics</subject><subject>Laboratory animals</subject><subject>Male</subject><subject>Metabolisms and neurohumoral controls</subject><subject>oxalic acid</subject><subject>Oxalic Acid - pharmacokinetics</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rodents</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>Water and mineral metabolism. Osmoregulation. Acidobasic balance</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkE1r3DAQhkVpSDdpb72GmBJ6ijcz-rCtY1jSNrCQQ7pnMZbloMVrbSQbkn9fpbs00NMMM8-8DA9jXxGWCFrcbMcb5HqJS6zhA1ugklhWCPCRLQA4LwVW1Sd2ltIWAFDq5pSdao2q0WrBVrdtCnE_-TAWoS8sDdbPuyK80ECTK7rgUjGGqYjuefYxD3xKwXr6e-DHItKUPrOTnobkvhzrOdv8uPu9-lWuH37er27XpZUgp7JrgHdWa6mloAqIoMJONVLpljvqnaxy27uuJaprKVxrOWgiLhroGtnW4px9P-TuY3ieXZrMzifrhoFGF-ZkKq0qJWqewW__gdswxzH_ZlDXUgqlMEPXB8jGkFJ0vdlHv6P4ahDMm1izHU0Wa9BksRm_OGbO7c51_-Cjyby_Ou4pZYt9pNH69J5Zc47QZOzygPUUDD3FjGweOaAAriHHoPgDVlGIag</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Hanes, D.A</creator><creator>Weaver, C.M</creator><creator>Heaney, R.P</creator><creator>Wastney, M</creator><general>American Society for Nutritional Sciences</general><general>American Institute of Nutrition</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>1999</creationdate><title>Absorption of calcium oxalate does not require dissociation in rats</title><author>Hanes, D.A ; Weaver, C.M ; Heaney, R.P ; Wastney, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-d802dc994943a60aa061d58459b2eafe46459fedbaa7743ebc209aa2380d84b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Absorption</topic><topic>animal models</topic><topic>Animals</topic><topic>bioavailability</topic><topic>Biological and medical sciences</topic><topic>blood serum</topic><topic>Calcium</topic><topic>Calcium - pharmacokinetics</topic><topic>calcium absorption</topic><topic>Calcium Oxalate - pharmacokinetics</topic><topic>calcium oxide</topic><topic>Calcium Radioisotopes</topic><topic>Carbon Radioisotopes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>intestinal absorption</topic><topic>kinetics</topic><topic>Laboratory animals</topic><topic>Male</topic><topic>Metabolisms and neurohumoral controls</topic><topic>oxalic acid</topic><topic>Oxalic Acid - pharmacokinetics</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rodents</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>Water and mineral metabolism. Osmoregulation. Acidobasic balance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hanes, D.A</creatorcontrib><creatorcontrib>Weaver, C.M</creatorcontrib><creatorcontrib>Heaney, R.P</creatorcontrib><creatorcontrib>Wastney, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hanes, D.A</au><au>Weaver, C.M</au><au>Heaney, R.P</au><au>Wastney, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Absorption of calcium oxalate does not require dissociation in rats</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>1999</date><risdate>1999</risdate><volume>129</volume><issue>1</issue><spage>170</spage><epage>173</epage><pages>170-173</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><coden>JONUAI</coden><abstract>Calcium absorption is thought to occur only if calcium is in a soluble or dissociated form, although experimental evidence is lacking. The intestinal absorption of calcium oxalate a small, neutral and virtually insoluble calcium salt, was elucidated in the whole body of awake rats. Suspensions of 45Ca ascorbate, 14C oxalic acid and doubly labeled 45Ca-[14C]-oxalate were given by gavage to separate groups of rats. Following dosing, blood samples were drawn for up to 240 min through a previously inserted intravenous catheter. Serum was assayed for radioactive tracers, and data were then plotted as fraction of dose over time. Calcium absorption was 15% [with a loading of 0.3 mmol (15 mg) calcium], oxalic acid absorption was 22% and Ca-oxalate absorption was <2%. Appearance of 45Ca from calcium ascorbate and 14C from oxalic acid differed, whereas 45Ca and 14C from doubly labeled Ca-oxalate had identical serum appearance profiles. Therefore, we conclude that calcium oxalate was absorbed intact. Addition of excess, unlabeled calcium to the doubly-labeled calcium oxalate did not alter the relationship of the serum level of the two tracers, confirming absorption of calcium oxalate as the intact salt. Thus, calcium bound as a small, neutral, calcium salt such as calcium oxalate does not have to be dissociated prior to absorption. Possibly other small compounds would be similarly absorbed. These results alter our current understanding of calcium bioavailability from foods and therapeutic agents.</abstract><cop>Bethesda, MD</cop><pub>American Society for Nutritional Sciences</pub><pmid>9915895</pmid><doi>10.1093/jn/129.1.170</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of nutrition, 1999, Vol.129 (1), p.170-173 |
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| subjects | Absorption animal models Animals bioavailability Biological and medical sciences blood serum Calcium Calcium - pharmacokinetics calcium absorption Calcium Oxalate - pharmacokinetics calcium oxide Calcium Radioisotopes Carbon Radioisotopes Fundamental and applied biological sciences. Psychology intestinal absorption kinetics Laboratory animals Male Metabolisms and neurohumoral controls oxalic acid Oxalic Acid - pharmacokinetics Rats Rats, Sprague-Dawley Rodents Vertebrates: anatomy and physiology, studies on body, several organs or systems Water and mineral metabolism. Osmoregulation. Acidobasic balance |
| title | Absorption of calcium oxalate does not require dissociation in rats |
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