Microsporidia Are Related to Fungi: Evidence from the Largest Subunit of RNA Polymerase II and Other Proteins
We have determined complete gene sequences encoding the largest subunit of the RNA polymerase II (RBP1) from two Microsporidia, Vairimorpha necatrix and Nosema locustae. Phylogenetic analyses of these and other RPB1 sequences strongly support the notion that Microsporidia are not early-diverging euk...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1999-01, Vol.96 (2), p.580-585 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Hirt, Robert P. Logsdon, John M. Healy, Bryan Dorey, Michael W. Doolittle, W. Ford Embley, T. Martin |
| description | We have determined complete gene sequences encoding the largest subunit of the RNA polymerase II (RBP1) from two Microsporidia, Vairimorpha necatrix and Nosema locustae. Phylogenetic analyses of these and other RPB1 sequences strongly support the notion that Microsporidia are not early-diverging eukaryotes but instead are specifically related to Fungi. Our reexamination of elongation factors EF-1α and EF-2 sequence data that had previously been taken as support for an early (Archezoan) divergence of these amitochondriate protists show such support to be weak and likely caused by artifacts in phylogenetic analyses. These EF data sets are, in fact, not inconsistent with a Microsporidia + Fungi relationship. In addition, we show that none of these proteins strongly support a deep divergence of Parabasalia and Metamonada, the other amitochondriate protist groups currently thought to compose early branches. Thus, the phylogenetic placement among eukaryotes for these protist taxa is in need of further critical examination. |
| doi_str_mv | 10.1073/pnas.96.2.580 |
| format | Article |
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Ford ; Embley, T. Martin</creator><creatorcontrib>Hirt, Robert P. ; Logsdon, John M. ; Healy, Bryan ; Dorey, Michael W. ; Doolittle, W. Ford ; Embley, T. Martin</creatorcontrib><description>We have determined complete gene sequences encoding the largest subunit of the RNA polymerase II (RBP1) from two Microsporidia, Vairimorpha necatrix and Nosema locustae. Phylogenetic analyses of these and other RPB1 sequences strongly support the notion that Microsporidia are not early-diverging eukaryotes but instead are specifically related to Fungi. Our reexamination of elongation factors EF-1α and EF-2 sequence data that had previously been taken as support for an early (Archezoan) divergence of these amitochondriate protists show such support to be weak and likely caused by artifacts in phylogenetic analyses. These EF data sets are, in fact, not inconsistent with a Microsporidia + Fungi relationship. In addition, we show that none of these proteins strongly support a deep divergence of Parabasalia and Metamonada, the other amitochondriate protist groups currently thought to compose early branches. Thus, the phylogenetic placement among eukaryotes for these protist taxa is in need of further critical examination.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.96.2.580</identifier><identifier>PMID: 9892676</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amino acids ; Animals ; Biological Sciences ; Datasets ; DNA Primers - genetics ; Ecology ; Eukaryotic cells ; Eukaryotic Cells - classification ; Fungi ; Fungi - genetics ; Genomics ; Giardia - genetics ; Glugea ; Microsporida - genetics ; Microsporidia ; Molecular Sequence Data ; Music analysis ; Nosema locustae ; Peptide Elongation Factor 1 ; Peptide Elongation Factors - genetics ; Phylogenetics ; Phylogeny ; Proteins ; Ribonucleic acid ; RNA ; RNA Polymerase II - genetics ; Sequence Alignment ; Sequence Analysis, DNA ; Trichomonas - genetics ; Vairimorpha necatrix ; We they distinction</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1999-01, Vol.96 (2), p.580-585</ispartof><rights>Copyright 1993-1999 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Jan 19, 1999</rights><rights>Copyright © 1999, The National Academy of Sciences 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c574t-9bacb4b1b5d8e13caff0347be3f31a5193af419971e5acd2eb9a09927a67c5863</citedby><cites>FETCH-LOGICAL-c574t-9bacb4b1b5d8e13caff0347be3f31a5193af419971e5acd2eb9a09927a67c5863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/96/2.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/46854$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/46854$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9892676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hirt, Robert P.</creatorcontrib><creatorcontrib>Logsdon, John M.</creatorcontrib><creatorcontrib>Healy, Bryan</creatorcontrib><creatorcontrib>Dorey, Michael W.</creatorcontrib><creatorcontrib>Doolittle, W. Ford</creatorcontrib><creatorcontrib>Embley, T. Martin</creatorcontrib><title>Microsporidia Are Related to Fungi: Evidence from the Largest Subunit of RNA Polymerase II and Other Proteins</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We have determined complete gene sequences encoding the largest subunit of the RNA polymerase II (RBP1) from two Microsporidia, Vairimorpha necatrix and Nosema locustae. Phylogenetic analyses of these and other RPB1 sequences strongly support the notion that Microsporidia are not early-diverging eukaryotes but instead are specifically related to Fungi. Our reexamination of elongation factors EF-1α and EF-2 sequence data that had previously been taken as support for an early (Archezoan) divergence of these amitochondriate protists show such support to be weak and likely caused by artifacts in phylogenetic analyses. These EF data sets are, in fact, not inconsistent with a Microsporidia + Fungi relationship. In addition, we show that none of these proteins strongly support a deep divergence of Parabasalia and Metamonada, the other amitochondriate protist groups currently thought to compose early branches. Thus, the phylogenetic placement among eukaryotes for these protist taxa is in need of further critical examination.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Biological Sciences</subject><subject>Datasets</subject><subject>DNA Primers - genetics</subject><subject>Ecology</subject><subject>Eukaryotic cells</subject><subject>Eukaryotic Cells - classification</subject><subject>Fungi</subject><subject>Fungi - genetics</subject><subject>Genomics</subject><subject>Giardia - genetics</subject><subject>Glugea</subject><subject>Microsporida - genetics</subject><subject>Microsporidia</subject><subject>Molecular Sequence Data</subject><subject>Music analysis</subject><subject>Nosema locustae</subject><subject>Peptide Elongation Factor 1</subject><subject>Peptide Elongation Factors - genetics</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Polymerase II - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Trichomonas - genetics</subject><subject>Vairimorpha necatrix</subject><subject>We they distinction</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAURSMEKtPCkg0CyUKCXQbb8UeM2IyqFkYaaFVgbTnJy9SjJB5sp2r_PY4mGgELWHlxzrXeezfLXhC8JFgW7_eDCUsllnTJS_woWxCsSC6Ywo-zBcZU5iWj7Gl2GsIOY6ySdJKdqFJRIcUi67_Y2ruwd9421qCVB3QDnYnQoOjQ5Ths7Qd0cWcbGGpArXc9ireANsZvIUT0bazGwUbkWnTzdYWuXffQgzcB0HqNzNCgq2R7dO1dBDuEZ9mT1nQBns_vWfbj8uL7-ed8c_Vpfb7a5DWXLOaqMnXFKlLxpgRS1KZtccFkBUVbEMOJKkzLiFKSADd1Q6FSBitFpRGy5qUozrKPh3_3Y9VDU8MQven03tve-AftjNV_ksHe6q2704QTqVL83Rz37ueY9tS9DTV0nRnAjUELxQWnBf6vSCRhilKaxDd_iTs3-iHdQFNMCllSNk2dH6SpkeChPQ5MsJ661lPXWglNdaox-a9_3_Joz-Um_nbmU-xI57hux66LcB-T9-ofXsIvD3gXovNHzkTJWfEL5h7GOg</recordid><startdate>19990119</startdate><enddate>19990119</enddate><creator>Hirt, Robert P.</creator><creator>Logsdon, John M.</creator><creator>Healy, Bryan</creator><creator>Dorey, Michael W.</creator><creator>Doolittle, W. 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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1999-01, Vol.96 (2), p.580-585 |
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| subjects | Amino acids Animals Biological Sciences Datasets DNA Primers - genetics Ecology Eukaryotic cells Eukaryotic Cells - classification Fungi Fungi - genetics Genomics Giardia - genetics Glugea Microsporida - genetics Microsporidia Molecular Sequence Data Music analysis Nosema locustae Peptide Elongation Factor 1 Peptide Elongation Factors - genetics Phylogenetics Phylogeny Proteins Ribonucleic acid RNA RNA Polymerase II - genetics Sequence Alignment Sequence Analysis, DNA Trichomonas - genetics Vairimorpha necatrix We they distinction |
| title | Microsporidia Are Related to Fungi: Evidence from the Largest Subunit of RNA Polymerase II and Other Proteins |
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