regA, a Volvox gene that plays a central role in germ-soma differentiation, encodes a novel regulatory protein
Volvox has two cell types: mortal somatic cells and immortal germ cells. Here we describe the transposon-tagging, cloning and characterization of regA, which plays a central role as a master regulatory gene in Volvox germ-soma differentiation by suppressing reproductive activities in somatic cells....
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| Veröffentlicht in: | Development (Cambridge) 1999-02, Vol.126 (4), p.639-647 |
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| creator | Kirk, M M Stark, K Miller, S M Müller, W Taillon, B E Gruber, H Schmitt, R Kirk, D L |
| description | Volvox has two cell types: mortal somatic cells and immortal germ cells. Here we describe the transposon-tagging, cloning and characterization of regA, which plays a central role as a master regulatory gene in Volvox germ-soma differentiation by suppressing reproductive activities in somatic cells. The 12.5 kb regA transcription unit generates a 6,725 nucleotide mRNA that appears at the beginning of somatic cell differentiation, and that encodes a 111 kDa RegA protein that localizes to the nucleus, and has an unusual abundance of alanine, glutamine and proline. This is a compositional feature shared by functional domains of many âactiveâ repressors. These findings are consistent with the hypothesis that RegA acts in somatic cells to repress transcription of genes required for growth and reproduction, including 13 genes whose products are required for chloroplast biogenesis. |
| doi_str_mv | 10.1242/dev.126.4.639 |
| format | Article |
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Here we describe the transposon-tagging, cloning and characterization of regA, which plays a central role as a master regulatory gene in Volvox germ-soma differentiation by suppressing reproductive activities in somatic cells. The 12.5 kb regA transcription unit generates a 6,725 nucleotide mRNA that appears at the beginning of somatic cell differentiation, and that encodes a 111 kDa RegA protein that localizes to the nucleus, and has an unusual abundance of alanine, glutamine and proline. This is a compositional feature shared by functional domains of many âactiveâ repressors. 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Here we describe the transposon-tagging, cloning and characterization of regA, which plays a central role as a master regulatory gene in Volvox germ-soma differentiation by suppressing reproductive activities in somatic cells. The 12.5 kb regA transcription unit generates a 6,725 nucleotide mRNA that appears at the beginning of somatic cell differentiation, and that encodes a 111 kDa RegA protein that localizes to the nucleus, and has an unusual abundance of alanine, glutamine and proline. This is a compositional feature shared by functional domains of many âactiveâ repressors. 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Stark, K ; Miller, S M ; Müller, W ; Taillon, B E ; Gruber, H ; Schmitt, R ; Kirk, D L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-ea31bf1b366bf6b78c6744e0a43a550853b9ffa7907cca03e6a8af4d08737d763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Algal Proteins - chemistry</topic><topic>Algal Proteins - genetics</topic><topic>Amino Acid Sequence</topic><topic>Cell Differentiation - genetics</topic><topic>Chlorophyta - genetics</topic><topic>Cloning, Molecular</topic><topic>DNA Transposable Elements - genetics</topic><topic>Freshwater</topic><topic>Gene Expression Regulation - genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation - genetics</topic><topic>Nuclear Proteins - genetics</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>regA gene</topic><topic>RegA protein</topic><topic>Repressor Proteins - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Transcription, Genetic - genetics</topic><topic>Volvox</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kirk, M M</creatorcontrib><creatorcontrib>Stark, K</creatorcontrib><creatorcontrib>Miller, S M</creatorcontrib><creatorcontrib>Müller, W</creatorcontrib><creatorcontrib>Taillon, B E</creatorcontrib><creatorcontrib>Gruber, H</creatorcontrib><creatorcontrib>Schmitt, R</creatorcontrib><creatorcontrib>Kirk, D L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Development (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kirk, M M</au><au>Stark, K</au><au>Miller, S M</au><au>Müller, W</au><au>Taillon, B E</au><au>Gruber, H</au><au>Schmitt, R</au><au>Kirk, D L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>regA, a Volvox gene that plays a central role in germ-soma differentiation, encodes a novel regulatory protein</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>Development</addtitle><date>1999-02-01</date><risdate>1999</risdate><volume>126</volume><issue>4</issue><spage>639</spage><epage>647</epage><pages>639-647</pages><issn>0950-1991</issn><eissn>1477-9129</eissn><abstract>Volvox has two cell types: mortal somatic cells and immortal germ cells. Here we describe the transposon-tagging, cloning and characterization of regA, which plays a central role as a master regulatory gene in Volvox germ-soma differentiation by suppressing reproductive activities in somatic cells. The 12.5 kb regA transcription unit generates a 6,725 nucleotide mRNA that appears at the beginning of somatic cell differentiation, and that encodes a 111 kDa RegA protein that localizes to the nucleus, and has an unusual abundance of alanine, glutamine and proline. This is a compositional feature shared by functional domains of many âactiveâ repressors. These findings are consistent with the hypothesis that RegA acts in somatic cells to repress transcription of genes required for growth and reproduction, including 13 genes whose products are required for chloroplast biogenesis.</abstract><cop>England</cop><pub>The Company of Biologists Limited</pub><pmid>9895312</pmid><doi>10.1242/dev.126.4.639</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-1991 |
| ispartof | Development (Cambridge), 1999-02, Vol.126 (4), p.639-647 |
| issn | 0950-1991 1477-9129 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Algal Proteins - chemistry Algal Proteins - genetics Amino Acid Sequence Cell Differentiation - genetics Chlorophyta - genetics Cloning, Molecular DNA Transposable Elements - genetics Freshwater Gene Expression Regulation - genetics Molecular Sequence Data Mutation - genetics Nuclear Proteins - genetics Polymorphism, Restriction Fragment Length regA gene RegA protein Repressor Proteins - genetics RNA, Messenger - genetics Sequence Analysis, DNA Transcription, Genetic - genetics Volvox |
| title | regA, a Volvox gene that plays a central role in germ-soma differentiation, encodes a novel regulatory protein |
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