Skeletal Bone Morphogenetic Proteins Suppress the Expression of Collagenase-3 by Rat Osteoblasts
Abstract Bone morphogenetic proteins (BMPs) are secreted by skeletal cells, induce the differentiation of mesenchymal cells into cells of the osteoblastic lineage, and increase their differentiated function. BMPs also decrease collagenase-3 expression by the osteoblast. We tested the autocrine role...
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| Veröffentlicht in: | Endocrinology (Philadelphia) 1999-02, Vol.140 (2), p.562-567 |
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| creator | Gazzerro, Elizabetta Rydziel, Sheila Canalis, Ernesto |
| description | Abstract
Bone morphogenetic proteins (BMPs) are secreted by skeletal cells, induce the differentiation of mesenchymal cells into cells of the osteoblastic lineage, and increase their differentiated function. BMPs also decrease collagenase-3 expression by the osteoblast. We tested the autocrine role of BMPs on collagenase-3 expression in osteoblast-enriched cells from fetal rat calvariae (Ob cells) by examining the effects of noggin, a specific inhibitor of BMP binding and function. Although collagenase-3 transcript expression declined in untreated Ob cells in culture over a 24-h period, BMP-2, -4, and -6 decreased collagenase-3 messenger RNA levels in cells treated for 2–24 h. The addition of noggin prevented the decrease of collagenase-3 transcripts in control cultures, opposed the inhibitory actions of BMP-2, and increased the levels of the protease in the culture medium. Noggin did not alter the decay of collagenase-3 messenger RNA in transcriptionally arrested cells, and it increased the levels of collagenase-3 heterogeneous nuclear RNA in Ob cells. In conclusion, noggin enhances the synthesis of collagenase-3 in osteoblasts, supporting the notion that BMPs act as autocrine suppressors of collagenase-3 in skeletal cells, an effect that may contribute to the maintenance of the bone matrix. |
| doi_str_mv | 10.1210/endo.140.2.6493 |
| format | Article |
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Bone morphogenetic proteins (BMPs) are secreted by skeletal cells, induce the differentiation of mesenchymal cells into cells of the osteoblastic lineage, and increase their differentiated function. BMPs also decrease collagenase-3 expression by the osteoblast. We tested the autocrine role of BMPs on collagenase-3 expression in osteoblast-enriched cells from fetal rat calvariae (Ob cells) by examining the effects of noggin, a specific inhibitor of BMP binding and function. Although collagenase-3 transcript expression declined in untreated Ob cells in culture over a 24-h period, BMP-2, -4, and -6 decreased collagenase-3 messenger RNA levels in cells treated for 2–24 h. The addition of noggin prevented the decrease of collagenase-3 transcripts in control cultures, opposed the inhibitory actions of BMP-2, and increased the levels of the protease in the culture medium. Noggin did not alter the decay of collagenase-3 messenger RNA in transcriptionally arrested cells, and it increased the levels of collagenase-3 heterogeneous nuclear RNA in Ob cells. In conclusion, noggin enhances the synthesis of collagenase-3 in osteoblasts, supporting the notion that BMPs act as autocrine suppressors of collagenase-3 in skeletal cells, an effect that may contribute to the maintenance of the bone matrix.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/endo.140.2.6493</identifier><identifier>PMID: 9927278</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Animals ; Autocrine signalling ; Bone matrix ; Bone morphogenetic protein 2 ; Bone Morphogenetic Proteins - antagonists & inhibitors ; Bone Morphogenetic Proteins - physiology ; Carrier Proteins ; Cell culture ; Cell differentiation ; Cell Nucleus - metabolism ; Cells, Cultured ; Collagen ; Collagenase ; Collagenases - genetics ; Collagenases - metabolism ; Drug Stability ; Fetuses ; Matrix Metalloproteinase 13 ; Mesenchyme ; mRNA ; Noggin protein ; Osteoblastogenesis ; Osteoblasts ; Osteoblasts - drug effects ; Osteoblasts - enzymology ; Proteins ; Proteins - pharmacology ; Rats ; RNA, Messenger - antagonists & inhibitors ; RNA, Messenger - chemistry ; RNA, Messenger - metabolism ; Skull - cytology ; Space life sciences ; Suppressors ; Transcription</subject><ispartof>Endocrinology (Philadelphia), 1999-02, Vol.140 (2), p.562-567</ispartof><rights>Copyright © 1999 by The Endocrine Society 1999</rights><rights>Copyright © 1999 by The Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3423-a98026f0f3fc1ef0247b55fd22156b4c10e68f986f03b0681989455e1529797e3</citedby><cites>FETCH-LOGICAL-c3423-a98026f0f3fc1ef0247b55fd22156b4c10e68f986f03b0681989455e1529797e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9927278$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gazzerro, Elizabetta</creatorcontrib><creatorcontrib>Rydziel, Sheila</creatorcontrib><creatorcontrib>Canalis, Ernesto</creatorcontrib><title>Skeletal Bone Morphogenetic Proteins Suppress the Expression of Collagenase-3 by Rat Osteoblasts</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>Abstract
Bone morphogenetic proteins (BMPs) are secreted by skeletal cells, induce the differentiation of mesenchymal cells into cells of the osteoblastic lineage, and increase their differentiated function. BMPs also decrease collagenase-3 expression by the osteoblast. We tested the autocrine role of BMPs on collagenase-3 expression in osteoblast-enriched cells from fetal rat calvariae (Ob cells) by examining the effects of noggin, a specific inhibitor of BMP binding and function. Although collagenase-3 transcript expression declined in untreated Ob cells in culture over a 24-h period, BMP-2, -4, and -6 decreased collagenase-3 messenger RNA levels in cells treated for 2–24 h. The addition of noggin prevented the decrease of collagenase-3 transcripts in control cultures, opposed the inhibitory actions of BMP-2, and increased the levels of the protease in the culture medium. Noggin did not alter the decay of collagenase-3 messenger RNA in transcriptionally arrested cells, and it increased the levels of collagenase-3 heterogeneous nuclear RNA in Ob cells. In conclusion, noggin enhances the synthesis of collagenase-3 in osteoblasts, supporting the notion that BMPs act as autocrine suppressors of collagenase-3 in skeletal cells, an effect that may contribute to the maintenance of the bone matrix.</description><subject>Animals</subject><subject>Autocrine signalling</subject><subject>Bone matrix</subject><subject>Bone morphogenetic protein 2</subject><subject>Bone Morphogenetic Proteins - antagonists & inhibitors</subject><subject>Bone Morphogenetic Proteins - physiology</subject><subject>Carrier Proteins</subject><subject>Cell culture</subject><subject>Cell differentiation</subject><subject>Cell Nucleus - metabolism</subject><subject>Cells, Cultured</subject><subject>Collagen</subject><subject>Collagenase</subject><subject>Collagenases - genetics</subject><subject>Collagenases - metabolism</subject><subject>Drug Stability</subject><subject>Fetuses</subject><subject>Matrix Metalloproteinase 13</subject><subject>Mesenchyme</subject><subject>mRNA</subject><subject>Noggin protein</subject><subject>Osteoblastogenesis</subject><subject>Osteoblasts</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - enzymology</subject><subject>Proteins</subject><subject>Proteins - pharmacology</subject><subject>Rats</subject><subject>RNA, Messenger - antagonists & inhibitors</subject><subject>RNA, Messenger - chemistry</subject><subject>RNA, Messenger - metabolism</subject><subject>Skull - cytology</subject><subject>Space life sciences</subject><subject>Suppressors</subject><subject>Transcription</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctLxDAQh4Mouj7OnoSA4EHomneaoy7rAxTFxzm23YlWu01NWtD_3qy7ePDiKQnzzW8yfAjtUzKmjJITaGd-TAUZs7EShq-hETVCZppqso5GhFCeacb0FtqO8S09hRB8E20awzTT-Qg9P7xDA33R4DPfAr7xoXv1L9BCX1f4Lvge6jbih6HrAsSI-1fA08-fe-1b7B2e-KYpUkMRIeO4_ML3RY9vYw--bIrYx1204Yomwt7q3EFP59PHyWV2fXtxNTm9ziouGM8KkxOmHHHcVRQcYUKXUroZY1SqUlSUgMqdyRPCS6JyavK0pwQqmdFGA99BR8vcLviPAWJv53WsIH2uBT9Eq4xURCj5L0g1U2k8SeDhH_DND6FNS1hOOZGMGZ4n6mRJVcHHGMDZLtTzInxZSuxCkV0oskmRZXahKHUcrHKHcg6zX37lJNWPl3U_dP-GfQPMApiq</recordid><startdate>199902</startdate><enddate>199902</enddate><creator>Gazzerro, Elizabetta</creator><creator>Rydziel, Sheila</creator><creator>Canalis, Ernesto</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199902</creationdate><title>Skeletal Bone Morphogenetic Proteins Suppress the Expression of Collagenase-3 by Rat Osteoblasts</title><author>Gazzerro, Elizabetta ; Rydziel, Sheila ; Canalis, Ernesto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3423-a98026f0f3fc1ef0247b55fd22156b4c10e68f986f03b0681989455e1529797e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Autocrine signalling</topic><topic>Bone matrix</topic><topic>Bone morphogenetic protein 2</topic><topic>Bone Morphogenetic Proteins - antagonists & inhibitors</topic><topic>Bone Morphogenetic Proteins - physiology</topic><topic>Carrier Proteins</topic><topic>Cell culture</topic><topic>Cell differentiation</topic><topic>Cell Nucleus - metabolism</topic><topic>Cells, Cultured</topic><topic>Collagen</topic><topic>Collagenase</topic><topic>Collagenases - genetics</topic><topic>Collagenases - metabolism</topic><topic>Drug Stability</topic><topic>Fetuses</topic><topic>Matrix Metalloproteinase 13</topic><topic>Mesenchyme</topic><topic>mRNA</topic><topic>Noggin protein</topic><topic>Osteoblastogenesis</topic><topic>Osteoblasts</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - enzymology</topic><topic>Proteins</topic><topic>Proteins - pharmacology</topic><topic>Rats</topic><topic>RNA, Messenger - antagonists & inhibitors</topic><topic>RNA, Messenger - chemistry</topic><topic>RNA, Messenger - metabolism</topic><topic>Skull - cytology</topic><topic>Space life sciences</topic><topic>Suppressors</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gazzerro, Elizabetta</creatorcontrib><creatorcontrib>Rydziel, Sheila</creatorcontrib><creatorcontrib>Canalis, Ernesto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gazzerro, Elizabetta</au><au>Rydziel, Sheila</au><au>Canalis, Ernesto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Skeletal Bone Morphogenetic Proteins Suppress the Expression of Collagenase-3 by Rat Osteoblasts</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>1999-02</date><risdate>1999</risdate><volume>140</volume><issue>2</issue><spage>562</spage><epage>567</epage><pages>562-567</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><abstract>Abstract
Bone morphogenetic proteins (BMPs) are secreted by skeletal cells, induce the differentiation of mesenchymal cells into cells of the osteoblastic lineage, and increase their differentiated function. BMPs also decrease collagenase-3 expression by the osteoblast. We tested the autocrine role of BMPs on collagenase-3 expression in osteoblast-enriched cells from fetal rat calvariae (Ob cells) by examining the effects of noggin, a specific inhibitor of BMP binding and function. Although collagenase-3 transcript expression declined in untreated Ob cells in culture over a 24-h period, BMP-2, -4, and -6 decreased collagenase-3 messenger RNA levels in cells treated for 2–24 h. The addition of noggin prevented the decrease of collagenase-3 transcripts in control cultures, opposed the inhibitory actions of BMP-2, and increased the levels of the protease in the culture medium. Noggin did not alter the decay of collagenase-3 messenger RNA in transcriptionally arrested cells, and it increased the levels of collagenase-3 heterogeneous nuclear RNA in Ob cells. In conclusion, noggin enhances the synthesis of collagenase-3 in osteoblasts, supporting the notion that BMPs act as autocrine suppressors of collagenase-3 in skeletal cells, an effect that may contribute to the maintenance of the bone matrix.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>9927278</pmid><doi>10.1210/endo.140.2.6493</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Autocrine signalling Bone matrix Bone morphogenetic protein 2 Bone Morphogenetic Proteins - antagonists & inhibitors Bone Morphogenetic Proteins - physiology Carrier Proteins Cell culture Cell differentiation Cell Nucleus - metabolism Cells, Cultured Collagen Collagenase Collagenases - genetics Collagenases - metabolism Drug Stability Fetuses Matrix Metalloproteinase 13 Mesenchyme mRNA Noggin protein Osteoblastogenesis Osteoblasts Osteoblasts - drug effects Osteoblasts - enzymology Proteins Proteins - pharmacology Rats RNA, Messenger - antagonists & inhibitors RNA, Messenger - chemistry RNA, Messenger - metabolism Skull - cytology Space life sciences Suppressors Transcription |
| title | Skeletal Bone Morphogenetic Proteins Suppress the Expression of Collagenase-3 by Rat Osteoblasts |
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