Novel Probes Showing Specific Fluorescence Enhancement on Binding to a Hexahistidine Tag

The introduction of hexahistidine (His tag) is widely used as a tool for affinity purification of recombinant proteins, since the His tag binds selectively to nickel–nitrilotriacetic acid (Ni2+–NTA) complex. To develop efficient “turn‐on” fluorescent probes for His‐tagged proteins, we adopted a fluo...

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Veröffentlicht in:	Chemistry : a European journal 2008-09, Vol.14 (26), p.8004-8012
Hauptverfasser:	Kamoto, Mie, Umezawa, Naoki, Kato, Nobuki, Higuchi, Tsunehiko
Format:	Artikel
Sprache:	eng
Schlagworte:	cobalt Fluorescent Dyes - chemistry fluorescent probes Histidine - chemistry Ions - chemistry Ligands Metals - chemistry molecular recognition Molecular Structure peptides Peptides - chemistry Proteins - chemistry sensors
Online-Zugang:	Volltext
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