Isothiocyanate Concentration in Kohlrabi (Brassica oleracea L. Var. gongylodes) Plants As Influenced by Sulfur and Nitrogen Supply
Glucosinolates (GSss) represent bioactive compounds of Brassica vegetables whose health-promoting effects merely stem from their breakdown products, particularly the isothiocyanates (ITCs), released after hydrolysis of GSs by myrosinase. GSs are occasionally discussed as transient S reservoirs, but...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2008-09, Vol.56 (18), p.8334-8342 |
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| creator | Gerendás, Jóska Breuning, Stephanie Stahl, Thorsten Mersch-Sundermann, Volker Mühling, Karl H |
| description | Glucosinolates (GSss) represent bioactive compounds of Brassica vegetables whose health-promoting effects merely stem from their breakdown products, particularly the isothiocyanates (ITCs), released after hydrolysis of GSs by myrosinase. GSs are occasionally discussed as transient S reservoirs, but little is known concerning the interactive effect of S and N supply on ITC concentrations. Therefore, kohlrabi plants were grown in a pot experiment with varied S (0.00, 0.05, and 0.20 g pot−1) and N (1, 2, and 4 g pot−1) supplies. Plant growth exhibited a classical nutrient response curve with respect to both S and N. The ITC profile of kohlrabi tubers was dominated by methylthiobutyl ITC (11−1350 µmol (g DM)−1), followed by sulforaphan (7−120 µmol (g DM)−1), phenylethyl ITC (5−34 µmol (g DM)−1), and allyl ITC (5−38 µmol (g DM)−1), resulting from the hydrolysis of glucoerucin, glucoraphanin, gluconasturtiin, and sinigrin, respectively. The ITC profile was in agreement with reported data, and concentrations of all ITCs were substantially reduced in response to increasing N and decreasing S supply. A growth-induced dilution effect could be ruled out in most cases, and the results do not support the hypothesis that GS acts as transient reservoir with respect to S. |
| doi_str_mv | 10.1021/jf800399x |
| format | Article |
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Var. gongylodes) Plants As Influenced by Sulfur and Nitrogen Supply</title><source>ACS Publications</source><source>MEDLINE</source><creator>Gerendás, Jóska ; Breuning, Stephanie ; Stahl, Thorsten ; Mersch-Sundermann, Volker ; Mühling, Karl H</creator><creatorcontrib>Gerendás, Jóska ; Breuning, Stephanie ; Stahl, Thorsten ; Mersch-Sundermann, Volker ; Mühling, Karl H</creatorcontrib><description>Glucosinolates (GSss) represent bioactive compounds of Brassica vegetables whose health-promoting effects merely stem from their breakdown products, particularly the isothiocyanates (ITCs), released after hydrolysis of GSs by myrosinase. GSs are occasionally discussed as transient S reservoirs, but little is known concerning the interactive effect of S and N supply on ITC concentrations. Therefore, kohlrabi plants were grown in a pot experiment with varied S (0.00, 0.05, and 0.20 g pot−1) and N (1, 2, and 4 g pot−1) supplies. Plant growth exhibited a classical nutrient response curve with respect to both S and N. The ITC profile of kohlrabi tubers was dominated by methylthiobutyl ITC (11−1350 µmol (g DM)−1), followed by sulforaphan (7−120 µmol (g DM)−1), phenylethyl ITC (5−34 µmol (g DM)−1), and allyl ITC (5−38 µmol (g DM)−1), resulting from the hydrolysis of glucoerucin, glucoraphanin, gluconasturtiin, and sinigrin, respectively. The ITC profile was in agreement with reported data, and concentrations of all ITCs were substantially reduced in response to increasing N and decreasing S supply. A growth-induced dilution effect could be ruled out in most cases, and the results do not support the hypothesis that GS acts as transient reservoir with respect to S.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf800399x</identifier><identifier>PMID: 18715015</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>allyl isothiocyanate ; Bioactive Constituents ; Biological and medical sciences ; Brassica - chemistry ; Brassica - growth & development ; Brassica oleracea var. gongylodes ; chemical concentration ; enzyme activity ; Fertilization ; food analysis ; food composition ; Food industries ; Fruit and vegetable industries ; Fundamental and applied biological sciences. Psychology ; glucosinolates ; Glycoside Hydrolases - metabolism ; isothiocyanates ; Isothiocyanates - analysis ; kohlrabi ; methylthiobutyl isothiocyanate ; myrosinase ; N/S ratio ; nitrogen ; Nitrogen - administration & dosage ; nutrient content ; nutritive value ; phenylethyl isothiocyanate ; plant growth ; plant nutrition ; Plant Tubers - chemistry ; pot culture ; sulforaphane ; sulfur ; Sulfur - administration & dosage ; thioglucosidase ; vegetable quality ; β-glucosidase</subject><ispartof>Journal of agricultural and food chemistry, 2008-09, Vol.56 (18), p.8334-8342</ispartof><rights>Copyright © 2008 American Chemical Society</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a471t-1bd466d5b9d8ac3448ef3031164888c219c7db2f0d42e7d98d4fc4fdd121c5d23</citedby><cites>FETCH-LOGICAL-a471t-1bd466d5b9d8ac3448ef3031164888c219c7db2f0d42e7d98d4fc4fdd121c5d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf800399x$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf800399x$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20680490$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18715015$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gerendás, Jóska</creatorcontrib><creatorcontrib>Breuning, Stephanie</creatorcontrib><creatorcontrib>Stahl, Thorsten</creatorcontrib><creatorcontrib>Mersch-Sundermann, Volker</creatorcontrib><creatorcontrib>Mühling, Karl H</creatorcontrib><title>Isothiocyanate Concentration in Kohlrabi (Brassica oleracea L. Var. gongylodes) Plants As Influenced by Sulfur and Nitrogen Supply</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Glucosinolates (GSss) represent bioactive compounds of Brassica vegetables whose health-promoting effects merely stem from their breakdown products, particularly the isothiocyanates (ITCs), released after hydrolysis of GSs by myrosinase. GSs are occasionally discussed as transient S reservoirs, but little is known concerning the interactive effect of S and N supply on ITC concentrations. Therefore, kohlrabi plants were grown in a pot experiment with varied S (0.00, 0.05, and 0.20 g pot−1) and N (1, 2, and 4 g pot−1) supplies. Plant growth exhibited a classical nutrient response curve with respect to both S and N. The ITC profile of kohlrabi tubers was dominated by methylthiobutyl ITC (11−1350 µmol (g DM)−1), followed by sulforaphan (7−120 µmol (g DM)−1), phenylethyl ITC (5−34 µmol (g DM)−1), and allyl ITC (5−38 µmol (g DM)−1), resulting from the hydrolysis of glucoerucin, glucoraphanin, gluconasturtiin, and sinigrin, respectively. The ITC profile was in agreement with reported data, and concentrations of all ITCs were substantially reduced in response to increasing N and decreasing S supply. A growth-induced dilution effect could be ruled out in most cases, and the results do not support the hypothesis that GS acts as transient reservoir with respect to S.</description><subject>allyl isothiocyanate</subject><subject>Bioactive Constituents</subject><subject>Biological and medical sciences</subject><subject>Brassica - chemistry</subject><subject>Brassica - growth & development</subject><subject>Brassica oleracea var. gongylodes</subject><subject>chemical concentration</subject><subject>enzyme activity</subject><subject>Fertilization</subject><subject>food analysis</subject><subject>food composition</subject><subject>Food industries</subject><subject>Fruit and vegetable industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glucosinolates</subject><subject>Glycoside Hydrolases - metabolism</subject><subject>isothiocyanates</subject><subject>Isothiocyanates - analysis</subject><subject>kohlrabi</subject><subject>methylthiobutyl isothiocyanate</subject><subject>myrosinase</subject><subject>N/S ratio</subject><subject>nitrogen</subject><subject>Nitrogen - administration & dosage</subject><subject>nutrient content</subject><subject>nutritive value</subject><subject>phenylethyl isothiocyanate</subject><subject>plant growth</subject><subject>plant nutrition</subject><subject>Plant Tubers - chemistry</subject><subject>pot culture</subject><subject>sulforaphane</subject><subject>sulfur</subject><subject>Sulfur - administration & dosage</subject><subject>thioglucosidase</subject><subject>vegetable quality</subject><subject>β-glucosidase</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0MtuEzEUBuARAtFQWPAC4A2ILibYHnvGs2wjLoEIipKyYGOd8SV1OrGDPSM1W54co0TphpUln0__sf-ieEnwlGBK3m-swLhq2_tHxYRwiktOiHhcTHAeloLX5Kx4ltIGYyx4g58WZ0Q0hGPCJ8WfeQrDrQtqDx4Gg2bBK-OHCIMLHjmPvobbPkLn0LurCCk5BSj0JoIygBZT9BPiFK2DX-_7oE26QNc9-CGhy4Tm3vajyXEadXu0HHs7RgReo29uiGFtfL7b7fr98-KJhT6ZF8fzvLj5-GE1-1wuvn-azy4XJbCGDCXpNKtrzbtWC1AVY8LYCleE1EwIoShpVaM7arFm1DS6FZpZxazWhBLFNa3Oi7eH3F0Mv0eTBrl1SZk-P9iEMcm65VxUgmd4cYAqhpSisXIX3RbiXhIs_xUuT4Vn--oYOnZbox_kseEM3hwBJAW9jeCVSydHcS0wa3F25cG5NJj70xzinaybquFydb2Uv358WXHKruQs-9cHbyFIWMecebOkmFR5KSctFQ-bQSW5CWP0ud3_fOEvfXGtVA</recordid><startdate>20080924</startdate><enddate>20080924</enddate><creator>Gerendás, Jóska</creator><creator>Breuning, Stephanie</creator><creator>Stahl, Thorsten</creator><creator>Mersch-Sundermann, Volker</creator><creator>Mühling, Karl H</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080924</creationdate><title>Isothiocyanate Concentration in Kohlrabi (Brassica oleracea L. Var. gongylodes) Plants As Influenced by Sulfur and Nitrogen Supply</title><author>Gerendás, Jóska ; Breuning, Stephanie ; Stahl, Thorsten ; Mersch-Sundermann, Volker ; Mühling, Karl H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a471t-1bd466d5b9d8ac3448ef3031164888c219c7db2f0d42e7d98d4fc4fdd121c5d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>allyl isothiocyanate</topic><topic>Bioactive Constituents</topic><topic>Biological and medical sciences</topic><topic>Brassica - chemistry</topic><topic>Brassica - growth & development</topic><topic>Brassica oleracea var. gongylodes</topic><topic>chemical concentration</topic><topic>enzyme activity</topic><topic>Fertilization</topic><topic>food analysis</topic><topic>food composition</topic><topic>Food industries</topic><topic>Fruit and vegetable industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glucosinolates</topic><topic>Glycoside Hydrolases - metabolism</topic><topic>isothiocyanates</topic><topic>Isothiocyanates - analysis</topic><topic>kohlrabi</topic><topic>methylthiobutyl isothiocyanate</topic><topic>myrosinase</topic><topic>N/S ratio</topic><topic>nitrogen</topic><topic>Nitrogen - administration & dosage</topic><topic>nutrient content</topic><topic>nutritive value</topic><topic>phenylethyl isothiocyanate</topic><topic>plant growth</topic><topic>plant nutrition</topic><topic>Plant Tubers - chemistry</topic><topic>pot culture</topic><topic>sulforaphane</topic><topic>sulfur</topic><topic>Sulfur - administration & dosage</topic><topic>thioglucosidase</topic><topic>vegetable quality</topic><topic>β-glucosidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gerendás, Jóska</creatorcontrib><creatorcontrib>Breuning, Stephanie</creatorcontrib><creatorcontrib>Stahl, Thorsten</creatorcontrib><creatorcontrib>Mersch-Sundermann, Volker</creatorcontrib><creatorcontrib>Mühling, Karl H</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gerendás, Jóska</au><au>Breuning, Stephanie</au><au>Stahl, Thorsten</au><au>Mersch-Sundermann, Volker</au><au>Mühling, Karl H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isothiocyanate Concentration in Kohlrabi (Brassica oleracea L. Var. gongylodes) Plants As Influenced by Sulfur and Nitrogen Supply</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2008-09-24</date><risdate>2008</risdate><volume>56</volume><issue>18</issue><spage>8334</spage><epage>8342</epage><pages>8334-8342</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Glucosinolates (GSss) represent bioactive compounds of Brassica vegetables whose health-promoting effects merely stem from their breakdown products, particularly the isothiocyanates (ITCs), released after hydrolysis of GSs by myrosinase. GSs are occasionally discussed as transient S reservoirs, but little is known concerning the interactive effect of S and N supply on ITC concentrations. Therefore, kohlrabi plants were grown in a pot experiment with varied S (0.00, 0.05, and 0.20 g pot−1) and N (1, 2, and 4 g pot−1) supplies. Plant growth exhibited a classical nutrient response curve with respect to both S and N. The ITC profile of kohlrabi tubers was dominated by methylthiobutyl ITC (11−1350 µmol (g DM)−1), followed by sulforaphan (7−120 µmol (g DM)−1), phenylethyl ITC (5−34 µmol (g DM)−1), and allyl ITC (5−38 µmol (g DM)−1), resulting from the hydrolysis of glucoerucin, glucoraphanin, gluconasturtiin, and sinigrin, respectively. The ITC profile was in agreement with reported data, and concentrations of all ITCs were substantially reduced in response to increasing N and decreasing S supply. A growth-induced dilution effect could be ruled out in most cases, and the results do not support the hypothesis that GS acts as transient reservoir with respect to S.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>18715015</pmid><doi>10.1021/jf800399x</doi><tpages>9</tpages></addata></record> |
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| subjects | allyl isothiocyanate Bioactive Constituents Biological and medical sciences Brassica - chemistry Brassica - growth & development Brassica oleracea var. gongylodes chemical concentration enzyme activity Fertilization food analysis food composition Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology glucosinolates Glycoside Hydrolases - metabolism isothiocyanates Isothiocyanates - analysis kohlrabi methylthiobutyl isothiocyanate myrosinase N/S ratio nitrogen Nitrogen - administration & dosage nutrient content nutritive value phenylethyl isothiocyanate plant growth plant nutrition Plant Tubers - chemistry pot culture sulforaphane sulfur Sulfur - administration & dosage thioglucosidase vegetable quality β-glucosidase |
| title | Isothiocyanate Concentration in Kohlrabi (Brassica oleracea L. Var. gongylodes) Plants As Influenced by Sulfur and Nitrogen Supply |
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