Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries
Recently a novel member of the transforming growth factor β (TGFβ) superfamily termed growth/differentiation factor-9 (GDF-9) was shown to be expressed in ovaries of mice and humans, and to be essential for normal follicular development beyond the primary (type 2) follicle stage in mice. In the pr...
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| Veröffentlicht in: | Biology of reproduction 1999-02, Vol.60 (2), p.381-386 |
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| creator | Bodensteiner, K.J Clay, C.M Moeller, C.L Sawyer, H.R |
| description | Recently a novel member of the transforming growth factor β (TGFβ) superfamily termed growth/differentiation factor-9 (GDF-9)
was shown to be expressed in ovaries of mice and humans, and to be essential for normal follicular development beyond the
primary (type 2) follicle stage in mice. In the present study, the gene for ovine GDF-9 was isolated and characterized, and
expression of GDF-9 mRNA in ovaries of domestic ruminants was examined. The predicted amino acid sequence of ovine GDF-9 is
77% and 66% homologous to human and mouse GDF-9, respectively. Specific hybridization using homologous 35 S-antisense probes was restricted to oocytes. In contrast to similar studies in mice in which GDF-9 was first detected beginning
at the primary (type 2) follicle stage, in ovine and bovine ovaries GDF-9 mRNA was expressed beginning at the primordial (type
1) follicle stage. The observed timing and pattern of GDF-9 expression in oocytes of domestic ruminants is consistent with
a role for GDF-9 in the initiation and maintenance of folliculogenesis in these species, and supports the general concept
that early stages of follicular growth and development are regulated by intraovarian factors. |
| doi_str_mv | 10.1095/biolreprod60.2.381 |
| format | Article |
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was shown to be expressed in ovaries of mice and humans, and to be essential for normal follicular development beyond the
primary (type 2) follicle stage in mice. In the present study, the gene for ovine GDF-9 was isolated and characterized, and
expression of GDF-9 mRNA in ovaries of domestic ruminants was examined. The predicted amino acid sequence of ovine GDF-9 is
77% and 66% homologous to human and mouse GDF-9, respectively. Specific hybridization using homologous 35 S-antisense probes was restricted to oocytes. In contrast to similar studies in mice in which GDF-9 was first detected beginning
at the primary (type 2) follicle stage, in ovine and bovine ovaries GDF-9 mRNA was expressed beginning at the primordial (type
1) follicle stage. The observed timing and pattern of GDF-9 expression in oocytes of domestic ruminants is consistent with
a role for GDF-9 in the initiation and maintenance of folliculogenesis in these species, and supports the general concept
that early stages of follicular growth and development are regulated by intraovarian factors.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod60.2.381</identifier><identifier>PMID: 9916005</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Animals ; Biological and medical sciences ; Bone Morphogenetic Protein 15 ; Cattle ; cloning ; Cloning, Molecular ; development ; DNA - analysis ; DNA - chemistry ; Female ; Fundamental and applied biological sciences. Psychology ; gdf-9 gene ; genbank/af078545 ; Gene Expression ; Graafian follicles ; Growth Differentiation Factor 9 ; growth factors ; Growth Substances - chemistry ; Growth Substances - genetics ; Humans ; In Situ Hybridization ; Intercellular Signaling Peptides and Proteins ; localization ; Mammalian female genital system ; messenger RNA ; Mice ; Molecular Sequence Data ; Morphology. Physiology ; nucleotide sequences ; oocytes ; Ovary - metabolism ; Restriction Mapping ; RNA, Messenger - analysis ; Sequence Homology ; Sheep ; structural genes ; temporal variation ; Transforming Growth Factor beta - genetics ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1999-02, Vol.60 (2), p.381-386</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,776,780,785,786,23910,23911,25119,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1717650$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9916005$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bodensteiner, K.J</creatorcontrib><creatorcontrib>Clay, C.M</creatorcontrib><creatorcontrib>Moeller, C.L</creatorcontrib><creatorcontrib>Sawyer, H.R</creatorcontrib><title>Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Recently a novel member of the transforming growth factor β (TGFβ) superfamily termed growth/differentiation factor-9 (GDF-9)
was shown to be expressed in ovaries of mice and humans, and to be essential for normal follicular development beyond the
primary (type 2) follicle stage in mice. In the present study, the gene for ovine GDF-9 was isolated and characterized, and
expression of GDF-9 mRNA in ovaries of domestic ruminants was examined. The predicted amino acid sequence of ovine GDF-9 is
77% and 66% homologous to human and mouse GDF-9, respectively. Specific hybridization using homologous 35 S-antisense probes was restricted to oocytes. In contrast to similar studies in mice in which GDF-9 was first detected beginning
at the primary (type 2) follicle stage, in ovine and bovine ovaries GDF-9 mRNA was expressed beginning at the primordial (type
1) follicle stage. The observed timing and pattern of GDF-9 expression in oocytes of domestic ruminants is consistent with
a role for GDF-9 in the initiation and maintenance of folliculogenesis in these species, and supports the general concept
that early stages of follicular growth and development are regulated by intraovarian factors.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Morphogenetic Protein 15</subject><subject>Cattle</subject><subject>cloning</subject><subject>Cloning, Molecular</subject><subject>development</subject><subject>DNA - analysis</subject><subject>DNA - chemistry</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gdf-9 gene</subject><subject>genbank/af078545</subject><subject>Gene Expression</subject><subject>Graafian follicles</subject><subject>Growth Differentiation Factor 9</subject><subject>growth factors</subject><subject>Growth Substances - chemistry</subject><subject>Growth Substances - genetics</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Intercellular Signaling Peptides and Proteins</subject><subject>localization</subject><subject>Mammalian female genital system</subject><subject>messenger RNA</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Morphology. Physiology</subject><subject>nucleotide sequences</subject><subject>oocytes</subject><subject>Ovary - metabolism</subject><subject>Restriction Mapping</subject><subject>RNA, Messenger - analysis</subject><subject>Sequence Homology</subject><subject>Sheep</subject><subject>structural genes</subject><subject>temporal variation</subject><subject>Transforming Growth Factor beta - genetics</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuO1DAQRS0EGpqBH0BCZAHs0lO2O34s0YiXNIgFzDqqOHbayG03dnoCn8Ef41FHbGdVJd1TZ3GLkJcUthR0dzX4FLI95jQK2LItV_QR2dCO6VYyoR6TDQCIlnPBn5JnpfwEoDvO-AW50JoKgG5D_n5NwZpTwNyYkKKPU5NcM-9tk-58tM2U0zLvr0bvnM02zh5nn2Lj0Mwpt7qZbIUwjo39fcy2lPuwCh4683H1358O5zXdYfa2PCdPHIZiX6zzktx-_PDj-nN78-3Tl-v3N63jDOaWodKSS2DSjJTjiMitoKYTcuTDDtQARhmlKcgBB-nMrqNiRA2MKlSKI78k787e2t-vky1zf_DF2BAw2nQqvdBdp2rND4JUMsqp2lXw1QqehoMd-2P2B8x_-rXsmr9ZcywGg8sYjS__MSqpFB1U7O0Z2_tpv_hs-3LAEKqU98uyCOhZX19duddnzmHqccpVdfudAeXAlO60VvwfIU2ojQ</recordid><startdate>19990201</startdate><enddate>19990201</enddate><creator>Bodensteiner, K.J</creator><creator>Clay, C.M</creator><creator>Moeller, C.L</creator><creator>Sawyer, H.R</creator><general>Society for the Study of Reproduction</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990201</creationdate><title>Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries</title><author>Bodensteiner, K.J ; Clay, C.M ; Moeller, C.L ; Sawyer, H.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f320t-2a89737027cd13adaa3e61c567d3b408b0c8c89107bab7fc4516da90218a883a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Morphogenetic Protein 15</topic><topic>Cattle</topic><topic>cloning</topic><topic>Cloning, Molecular</topic><topic>development</topic><topic>DNA - analysis</topic><topic>DNA - chemistry</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gdf-9 gene</topic><topic>genbank/af078545</topic><topic>Gene Expression</topic><topic>Graafian follicles</topic><topic>Growth Differentiation Factor 9</topic><topic>growth factors</topic><topic>Growth Substances - chemistry</topic><topic>Growth Substances - genetics</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Intercellular Signaling Peptides and Proteins</topic><topic>localization</topic><topic>Mammalian female genital system</topic><topic>messenger RNA</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Morphology. Physiology</topic><topic>nucleotide sequences</topic><topic>oocytes</topic><topic>Ovary - metabolism</topic><topic>Restriction Mapping</topic><topic>RNA, Messenger - analysis</topic><topic>Sequence Homology</topic><topic>Sheep</topic><topic>structural genes</topic><topic>temporal variation</topic><topic>Transforming Growth Factor beta - genetics</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bodensteiner, K.J</creatorcontrib><creatorcontrib>Clay, C.M</creatorcontrib><creatorcontrib>Moeller, C.L</creatorcontrib><creatorcontrib>Sawyer, H.R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bodensteiner, K.J</au><au>Clay, C.M</au><au>Moeller, C.L</au><au>Sawyer, H.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1999-02-01</date><risdate>1999</risdate><volume>60</volume><issue>2</issue><spage>381</spage><epage>386</epage><pages>381-386</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Recently a novel member of the transforming growth factor β (TGFβ) superfamily termed growth/differentiation factor-9 (GDF-9)
was shown to be expressed in ovaries of mice and humans, and to be essential for normal follicular development beyond the
primary (type 2) follicle stage in mice. In the present study, the gene for ovine GDF-9 was isolated and characterized, and
expression of GDF-9 mRNA in ovaries of domestic ruminants was examined. The predicted amino acid sequence of ovine GDF-9 is
77% and 66% homologous to human and mouse GDF-9, respectively. Specific hybridization using homologous 35 S-antisense probes was restricted to oocytes. In contrast to similar studies in mice in which GDF-9 was first detected beginning
at the primary (type 2) follicle stage, in ovine and bovine ovaries GDF-9 mRNA was expressed beginning at the primordial (type
1) follicle stage. The observed timing and pattern of GDF-9 expression in oocytes of domestic ruminants is consistent with
a role for GDF-9 in the initiation and maintenance of folliculogenesis in these species, and supports the general concept
that early stages of follicular growth and development are regulated by intraovarian factors.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>9916005</pmid><doi>10.1095/biolreprod60.2.381</doi><tpages>6</tpages></addata></record> |
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| subjects | Amino Acid Sequence amino acid sequences Animals Biological and medical sciences Bone Morphogenetic Protein 15 Cattle cloning Cloning, Molecular development DNA - analysis DNA - chemistry Female Fundamental and applied biological sciences. Psychology gdf-9 gene genbank/af078545 Gene Expression Graafian follicles Growth Differentiation Factor 9 growth factors Growth Substances - chemistry Growth Substances - genetics Humans In Situ Hybridization Intercellular Signaling Peptides and Proteins localization Mammalian female genital system messenger RNA Mice Molecular Sequence Data Morphology. Physiology nucleotide sequences oocytes Ovary - metabolism Restriction Mapping RNA, Messenger - analysis Sequence Homology Sheep structural genes temporal variation Transforming Growth Factor beta - genetics Vertebrates: reproduction |
| title | Molecular cloning of the ovine growth/differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries |
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