Plasmodium berghei : Identification of an mdr-like gene associated with drug resistance
Amplification, mutations, or overexpression of the pfmdr1 gene have been associated with multiple drug resistance in some strains of Plasmodium falciparum. In order to better understand this potential mechanism of drug resistance, we are currently investigating putative mdr homologues in vivo in the...
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| Veröffentlicht in: | Experimental parasitology 1999, Vol.91 (1), p.86-92 |
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| creator | GERVAIS, G. W TRUJILLO, K ROBINSON, B. L PETERS, W SERRANO, A. E |
| description | Amplification, mutations, or overexpression of the pfmdr1 gene have been associated with multiple drug resistance in some strains of Plasmodium falciparum. In order to better understand this potential mechanism of drug resistance, we are currently investigating putative mdr homologues in vivo in the rodent malaria Plasmodium berghei. We have identified and partially sequenced a gene that is amplified in a MFQ-resistant (MFQr) line. Using degenerate primers, a 579-bp fragment was amplified by PCR using P. berghei genomic DNA as template. The predicted amino acid sequence shares 66% identity with the previously reported pfmdr1 gene product (Pgh1) of P. falciparum. Southern blots and slot blots of genomic DNA suggest that this gene is amplified two- to threefold in a MFQr line (N/1100), as has been previously reported in some MFQr strains of P. falciparum. The P. berghei gene was mapped to chromosome 12 in all of the lines analyzed. Furthermore, the cloned PCR product also hybridizes to chromosome 5 of the MFQr strain. |
| doi_str_mv | 10.1006/expr.1999.4344 |
| format | Article |
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W ; TRUJILLO, K ; ROBINSON, B. L ; PETERS, W ; SERRANO, A. E</creator><creatorcontrib>GERVAIS, G. W ; TRUJILLO, K ; ROBINSON, B. L ; PETERS, W ; SERRANO, A. E</creatorcontrib><description>Amplification, mutations, or overexpression of the pfmdr1 gene have been associated with multiple drug resistance in some strains of Plasmodium falciparum. In order to better understand this potential mechanism of drug resistance, we are currently investigating putative mdr homologues in vivo in the rodent malaria Plasmodium berghei. We have identified and partially sequenced a gene that is amplified in a MFQ-resistant (MFQr) line. Using degenerate primers, a 579-bp fragment was amplified by PCR using P. berghei genomic DNA as template. The predicted amino acid sequence shares 66% identity with the previously reported pfmdr1 gene product (Pgh1) of P. falciparum. Southern blots and slot blots of genomic DNA suggest that this gene is amplified two- to threefold in a MFQr line (N/1100), as has been previously reported in some MFQr strains of P. falciparum. The P. berghei gene was mapped to chromosome 12 in all of the lines analyzed. Furthermore, the cloned PCR product also hybridizes to chromosome 5 of the MFQr strain.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1006/expr.1999.4344</identifier><identifier>PMID: 9920046</identifier><identifier>CODEN: EXPAAA</identifier><language>eng</language><publisher>San Diego, CA: Elsevier</publisher><subject>Amino Acid Sequence ; Animals ; Antimalarials - pharmacology ; ATP-Binding Cassette Transporters ; Biochemistry. Physiology. Immunology. Molecular biology ; Biological and medical sciences ; Chromosome Mapping ; Cloning, Molecular ; Drug Resistance, Multiple - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Amplification ; Gene Dosage ; Genes, Protozoan ; Mefloquine - pharmacology ; Molecular Sequence Data ; Plasmodium berghei ; Plasmodium berghei - chemistry ; Plasmodium berghei - drug effects ; Plasmodium berghei - genetics ; Plasmodium falciparum ; Polymerase Chain Reaction ; Protozoa ; Protozoan Proteins - chemistry ; Protozoan Proteins - genetics ; Sequence Alignment</subject><ispartof>Experimental parasitology, 1999, Vol.91 (1), p.86-92</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1730441$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9920046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GERVAIS, G. W</creatorcontrib><creatorcontrib>TRUJILLO, K</creatorcontrib><creatorcontrib>ROBINSON, B. L</creatorcontrib><creatorcontrib>PETERS, W</creatorcontrib><creatorcontrib>SERRANO, A. E</creatorcontrib><title>Plasmodium berghei : Identification of an mdr-like gene associated with drug resistance</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>Amplification, mutations, or overexpression of the pfmdr1 gene have been associated with multiple drug resistance in some strains of Plasmodium falciparum. In order to better understand this potential mechanism of drug resistance, we are currently investigating putative mdr homologues in vivo in the rodent malaria Plasmodium berghei. We have identified and partially sequenced a gene that is amplified in a MFQ-resistant (MFQr) line. Using degenerate primers, a 579-bp fragment was amplified by PCR using P. berghei genomic DNA as template. The predicted amino acid sequence shares 66% identity with the previously reported pfmdr1 gene product (Pgh1) of P. falciparum. Southern blots and slot blots of genomic DNA suggest that this gene is amplified two- to threefold in a MFQr line (N/1100), as has been previously reported in some MFQr strains of P. falciparum. The P. berghei gene was mapped to chromosome 12 in all of the lines analyzed. Furthermore, the cloned PCR product also hybridizes to chromosome 5 of the MFQr strain.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antimalarials - pharmacology</subject><subject>ATP-Binding Cassette Transporters</subject><subject>Biochemistry. Physiology. Immunology. Molecular biology</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular</subject><subject>Drug Resistance, Multiple - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Amplification</subject><subject>Gene Dosage</subject><subject>Genes, Protozoan</subject><subject>Mefloquine - pharmacology</subject><subject>Molecular Sequence Data</subject><subject>Plasmodium berghei</subject><subject>Plasmodium berghei - chemistry</subject><subject>Plasmodium berghei - drug effects</subject><subject>Plasmodium berghei - genetics</subject><subject>Plasmodium falciparum</subject><subject>Polymerase Chain Reaction</subject><subject>Protozoa</subject><subject>Protozoan Proteins - chemistry</subject><subject>Protozoan Proteins - genetics</subject><subject>Sequence Alignment</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtLBDEUhYMo67ra2gkpxG7Wm-TOI3ay-AJBC8VyyePOGp3Hmsyg_nsXXGytTvF9HDiHsWMBcwFQnNPXOs6F1nqOCnGHTQVoyCSi3mVTAIEZVhr32UFKbwBQCYkTNtFaAmAxZS-PjUlt78PYcktx9UqBX_A7T90Q6uDMEPqO9zU3HW99zJrwTnxFHXGTUu-CGcjzzzC8ch_HFY-UQhpM5-iQ7dWmSXS0zRl7vr56Wtxm9w83d4vL-2wti2LIvJNYgc2dktIKlWs0kozyUBCR2qywsnK2Rl36WlqAXDkpdWG1tghKGjVjZ7-969h_jJSGZRuSo6YxHfVjWhY6z8tSlf-KohRlpUrciCdbcbQt-eU6htbE7-X2sg0_3XKTnGnquJkb0p8mSgWIQv0AIOh6OA</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>GERVAIS, G. W</creator><creator>TRUJILLO, K</creator><creator>ROBINSON, B. L</creator><creator>PETERS, W</creator><creator>SERRANO, A. E</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1999</creationdate><title>Plasmodium berghei : Identification of an mdr-like gene associated with drug resistance</title><author>GERVAIS, G. W ; TRUJILLO, K ; ROBINSON, B. L ; PETERS, W ; SERRANO, A. E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-dc2480b5c322b13594a2ea3d06eee3244b28cbf497df2b0053c2296b99b4032a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antimalarials - pharmacology</topic><topic>ATP-Binding Cassette Transporters</topic><topic>Biochemistry. Physiology. Immunology. Molecular biology</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>Cloning, Molecular</topic><topic>Drug Resistance, Multiple - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Amplification</topic><topic>Gene Dosage</topic><topic>Genes, Protozoan</topic><topic>Mefloquine - pharmacology</topic><topic>Molecular Sequence Data</topic><topic>Plasmodium berghei</topic><topic>Plasmodium berghei - chemistry</topic><topic>Plasmodium berghei - drug effects</topic><topic>Plasmodium berghei - genetics</topic><topic>Plasmodium falciparum</topic><topic>Polymerase Chain Reaction</topic><topic>Protozoa</topic><topic>Protozoan Proteins - chemistry</topic><topic>Protozoan Proteins - genetics</topic><topic>Sequence Alignment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GERVAIS, G. W</creatorcontrib><creatorcontrib>TRUJILLO, K</creatorcontrib><creatorcontrib>ROBINSON, B. L</creatorcontrib><creatorcontrib>PETERS, W</creatorcontrib><creatorcontrib>SERRANO, A. 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E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasmodium berghei : Identification of an mdr-like gene associated with drug resistance</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>1999</date><risdate>1999</risdate><volume>91</volume><issue>1</issue><spage>86</spage><epage>92</epage><pages>86-92</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>Amplification, mutations, or overexpression of the pfmdr1 gene have been associated with multiple drug resistance in some strains of Plasmodium falciparum. In order to better understand this potential mechanism of drug resistance, we are currently investigating putative mdr homologues in vivo in the rodent malaria Plasmodium berghei. We have identified and partially sequenced a gene that is amplified in a MFQ-resistant (MFQr) line. Using degenerate primers, a 579-bp fragment was amplified by PCR using P. berghei genomic DNA as template. The predicted amino acid sequence shares 66% identity with the previously reported pfmdr1 gene product (Pgh1) of P. falciparum. Southern blots and slot blots of genomic DNA suggest that this gene is amplified two- to threefold in a MFQr line (N/1100), as has been previously reported in some MFQr strains of P. falciparum. The P. berghei gene was mapped to chromosome 12 in all of the lines analyzed. Furthermore, the cloned PCR product also hybridizes to chromosome 5 of the MFQr strain.</abstract><cop>San Diego, CA</cop><pub>Elsevier</pub><pmid>9920046</pmid><doi>10.1006/expr.1999.4344</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Antimalarials - pharmacology ATP-Binding Cassette Transporters Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Chromosome Mapping Cloning, Molecular Drug Resistance, Multiple - genetics Fundamental and applied biological sciences. Psychology Gene Amplification Gene Dosage Genes, Protozoan Mefloquine - pharmacology Molecular Sequence Data Plasmodium berghei Plasmodium berghei - chemistry Plasmodium berghei - drug effects Plasmodium berghei - genetics Plasmodium falciparum Polymerase Chain Reaction Protozoa Protozoan Proteins - chemistry Protozoan Proteins - genetics Sequence Alignment |
| title | Plasmodium berghei : Identification of an mdr-like gene associated with drug resistance |
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