Preparation of Recombinant Gilthead Seabream (Sparus aurata) Growth Hormone and Its Use for Stimulation of Larvae Growth by Oral Administration

Gilthead seabream (Sparus aurata) growth hormone (gsGH) cDNA coding for the mature protein was cloned in a pGEM-T vector and then transferred into prokaryotic expression vector pET-8 and expressed inE. coliBL21 (DE3) cells upon induction with IPTG. The expressed protein, contained within the inclusi...

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Veröffentlicht in:	General and comparative endocrinology 1999-01, Vol.113 (1), p.155-164
Hauptverfasser:	Ben-Atia, I, Fine, M, Tandler, A, Funkenstein, B, Maurice, S, Cavari, B, Gertler, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Administration, Oral Animals beta-Lactamases - metabolism Electrophoresis, Polyacrylamide Gel gilthead seabream Growth - drug effects growth hormone Growth Hormone - biosynthesis Growth Hormone - pharmacology Iodine Radioisotopes Larva Microsomes, Liver - drug effects Microsomes, Liver - metabolism oral administration Perciformes - metabolism recombinant Recombinant Proteins - biosynthesis Recombinant Proteins - pharmacology Sparus aurata
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