WNT5A Expression Increases during Melanoma Progression and Correlates with Outcome
Purpose: Wnt ligands play a major role in development and are important in cancer. Expression microarray analysis correlates one member of this family, WNT5A, to a subclass of melanomas with increased motility and invasion. There are no large studies of clinical samples primarily addressing the impo...
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| Veröffentlicht in: | Clinical cancer research 2008-09, Vol.14 (18), p.5825-5832 |
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| creator | DA FORNO, Philip D PRINGLE, J. Howard HUTCHINSON, Peter OSBORN, Joy QIANG HUANG POTTER, Linda HANCOX, Rachael A FLETCHER, Alan SALDANHA, Gerald S |
| description | Purpose: Wnt ligands play a major role in development and are important in cancer. Expression microarray analysis correlates one member
of this family, WNT5A, to a subclass of melanomas with increased motility and invasion. There are no large studies of clinical
samples primarily addressing the importance of WNT5A in melanoma progression or outcome. Therefore, this study aimed to assess
the protein expression of WNT5A during melanoma progression and its effect on outcome.
Experimental Design: Expression of WNT5A was determined in a series of 59 primary melanomas with matched metastases. To provide a benchmark of
progression against which to assess WNT5A, expression of p16 ink4a was analyzed, as this has been previously well documented in melanoma. The effect of WNT5A protein expression on outcome
was assessed in 102 melanomas.
Results: Cytoplasmic WNT5A showed a trend of increasing expression with melanoma progression ( P = 0.013), whereas there was diminishing p16 ink4a expression ( P = 0.006). Nevi showed relatively strong WNT5A expression. Strong cytoplasmic WNT5A was an independent risk factor for reduced
metastasis-free and overall survival in multivariate analysis ( P = 0.001 and 0.003, respectively).
Conclusion: Cytoplasmic WNT5A increases with melanoma progression and strong expression is associated with poor outcome. |
| doi_str_mv | 10.1158/1078-0432.CCR-07-5104 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69553983</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69553983</sourcerecordid><originalsourceid>FETCH-LOGICAL-c371t-dec796ede4c7b9b38e3aa73450b34bcd4c5aea4dc67fe965582b05a29c7a11bc3</originalsourceid><addsrcrecordid>eNpFkNFOwjAUhhujEUQfQbMbTbwYtmu7rpdkQSVBMQTjZdN1B5hhG7Zb0Le3C6BXp8n5_r_th9A1wUNCePJAsEhCzGg0TNN5iEXICWYnqE84FyGNYn7qz0emhy6c-8SYMA-dox5JhGRY0j6af7wu-CgYf28tOFfUVTCpjAXtwAV5a4tqFbzARld1qYM3W6-OlK7yIK2t9bvGo7uiWQeztjF1CZfobKk3Dq4Oc4DeH8eL9Dmczp4m6WgaGipIE-ZghIwhB2ZEJjOaANVaUMZxRllmcma4Bs1yE4slyJjzJMow15E0QhOSGTpAd_vera2_WnCNKgtnYONfC3XrVCw5pzKhHuR70NjaOQtLtbVFqe2PIlh1MlUnSnWilJepsFCdTJ-7OVzQZiXk_6mDPQ_cHgDtjN4sra5M4f64CEsZEdIV3e-5dbFa7woLyngSvD0H2pq1IsyXKv9DTn8BbtCMLA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69553983</pqid></control><display><type>article</type><title>WNT5A Expression Increases during Melanoma Progression and Correlates with Outcome</title><source>MEDLINE</source><source>American Association for Cancer Research</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>DA FORNO, Philip D ; PRINGLE, J. Howard ; HUTCHINSON, Peter ; OSBORN, Joy ; QIANG HUANG ; POTTER, Linda ; HANCOX, Rachael A ; FLETCHER, Alan ; SALDANHA, Gerald S</creator><creatorcontrib>DA FORNO, Philip D ; PRINGLE, J. Howard ; HUTCHINSON, Peter ; OSBORN, Joy ; QIANG HUANG ; POTTER, Linda ; HANCOX, Rachael A ; FLETCHER, Alan ; SALDANHA, Gerald S</creatorcontrib><description>Purpose: Wnt ligands play a major role in development and are important in cancer. Expression microarray analysis correlates one member
of this family, WNT5A, to a subclass of melanomas with increased motility and invasion. There are no large studies of clinical
samples primarily addressing the importance of WNT5A in melanoma progression or outcome. Therefore, this study aimed to assess
the protein expression of WNT5A during melanoma progression and its effect on outcome.
Experimental Design: Expression of WNT5A was determined in a series of 59 primary melanomas with matched metastases. To provide a benchmark of
progression against which to assess WNT5A, expression of p16 ink4a was analyzed, as this has been previously well documented in melanoma. The effect of WNT5A protein expression on outcome
was assessed in 102 melanomas.
Results: Cytoplasmic WNT5A showed a trend of increasing expression with melanoma progression ( P = 0.013), whereas there was diminishing p16 ink4a expression ( P = 0.006). Nevi showed relatively strong WNT5A expression. Strong cytoplasmic WNT5A was an independent risk factor for reduced
metastasis-free and overall survival in multivariate analysis ( P = 0.001 and 0.003, respectively).
Conclusion: Cytoplasmic WNT5A increases with melanoma progression and strong expression is associated with poor outcome.</description><identifier>ISSN: 1078-0432</identifier><identifier>EISSN: 1557-3265</identifier><identifier>DOI: 10.1158/1078-0432.CCR-07-5104</identifier><identifier>PMID: 18794093</identifier><identifier>CODEN: CCREF4</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Antineoplastic agents ; Biological and medical sciences ; Cell Line, Tumor ; Cyclin-Dependent Kinase Inhibitor p16 - metabolism ; Cytoplasm - metabolism ; Dermatology ; Disease Progression ; Female ; Humans ; Male ; Medical sciences ; melanoma ; Melanoma - metabolism ; Melanoma - pathology ; metastasis ; Middle Aged ; Neoplasm Metastasis ; Pharmacology. Drug treatments ; Prognosis ; progression ; Proto-Oncogene Proteins - metabolism ; Skin Neoplasms - metabolism ; Skin Neoplasms - pathology ; survival ; Survival Analysis ; Tumors of the skin and soft tissue. Premalignant lesions ; Wnt Proteins - metabolism ; Wnt-5a Protein ; WNT5A</subject><ispartof>Clinical cancer research, 2008-09, Vol.14 (18), p.5825-5832</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-dec796ede4c7b9b38e3aa73450b34bcd4c5aea4dc67fe965582b05a29c7a11bc3</citedby><cites>FETCH-LOGICAL-c371t-dec796ede4c7b9b38e3aa73450b34bcd4c5aea4dc67fe965582b05a29c7a11bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3343,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20992114$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18794093$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DA FORNO, Philip D</creatorcontrib><creatorcontrib>PRINGLE, J. Howard</creatorcontrib><creatorcontrib>HUTCHINSON, Peter</creatorcontrib><creatorcontrib>OSBORN, Joy</creatorcontrib><creatorcontrib>QIANG HUANG</creatorcontrib><creatorcontrib>POTTER, Linda</creatorcontrib><creatorcontrib>HANCOX, Rachael A</creatorcontrib><creatorcontrib>FLETCHER, Alan</creatorcontrib><creatorcontrib>SALDANHA, Gerald S</creatorcontrib><title>WNT5A Expression Increases during Melanoma Progression and Correlates with Outcome</title><title>Clinical cancer research</title><addtitle>Clin Cancer Res</addtitle><description>Purpose: Wnt ligands play a major role in development and are important in cancer. Expression microarray analysis correlates one member
of this family, WNT5A, to a subclass of melanomas with increased motility and invasion. There are no large studies of clinical
samples primarily addressing the importance of WNT5A in melanoma progression or outcome. Therefore, this study aimed to assess
the protein expression of WNT5A during melanoma progression and its effect on outcome.
Experimental Design: Expression of WNT5A was determined in a series of 59 primary melanomas with matched metastases. To provide a benchmark of
progression against which to assess WNT5A, expression of p16 ink4a was analyzed, as this has been previously well documented in melanoma. The effect of WNT5A protein expression on outcome
was assessed in 102 melanomas.
Results: Cytoplasmic WNT5A showed a trend of increasing expression with melanoma progression ( P = 0.013), whereas there was diminishing p16 ink4a expression ( P = 0.006). Nevi showed relatively strong WNT5A expression. Strong cytoplasmic WNT5A was an independent risk factor for reduced
metastasis-free and overall survival in multivariate analysis ( P = 0.001 and 0.003, respectively).
Conclusion: Cytoplasmic WNT5A increases with melanoma progression and strong expression is associated with poor outcome.</description><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor</subject><subject>Cyclin-Dependent Kinase Inhibitor p16 - metabolism</subject><subject>Cytoplasm - metabolism</subject><subject>Dermatology</subject><subject>Disease Progression</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>melanoma</subject><subject>Melanoma - metabolism</subject><subject>Melanoma - pathology</subject><subject>metastasis</subject><subject>Middle Aged</subject><subject>Neoplasm Metastasis</subject><subject>Pharmacology. Drug treatments</subject><subject>Prognosis</subject><subject>progression</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Skin Neoplasms - metabolism</subject><subject>Skin Neoplasms - pathology</subject><subject>survival</subject><subject>Survival Analysis</subject><subject>Tumors of the skin and soft tissue. Premalignant lesions</subject><subject>Wnt Proteins - metabolism</subject><subject>Wnt-5a Protein</subject><subject>WNT5A</subject><issn>1078-0432</issn><issn>1557-3265</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkNFOwjAUhhujEUQfQbMbTbwYtmu7rpdkQSVBMQTjZdN1B5hhG7Zb0Le3C6BXp8n5_r_th9A1wUNCePJAsEhCzGg0TNN5iEXICWYnqE84FyGNYn7qz0emhy6c-8SYMA-dox5JhGRY0j6af7wu-CgYf28tOFfUVTCpjAXtwAV5a4tqFbzARld1qYM3W6-OlK7yIK2t9bvGo7uiWQeztjF1CZfobKk3Dq4Oc4DeH8eL9Dmczp4m6WgaGipIE-ZghIwhB2ZEJjOaANVaUMZxRllmcma4Bs1yE4slyJjzJMow15E0QhOSGTpAd_vera2_WnCNKgtnYONfC3XrVCw5pzKhHuR70NjaOQtLtbVFqe2PIlh1MlUnSnWilJepsFCdTJ-7OVzQZiXk_6mDPQ_cHgDtjN4sra5M4f64CEsZEdIV3e-5dbFa7woLyngSvD0H2pq1IsyXKv9DTn8BbtCMLA</recordid><startdate>20080915</startdate><enddate>20080915</enddate><creator>DA FORNO, Philip D</creator><creator>PRINGLE, J. Howard</creator><creator>HUTCHINSON, Peter</creator><creator>OSBORN, Joy</creator><creator>QIANG HUANG</creator><creator>POTTER, Linda</creator><creator>HANCOX, Rachael A</creator><creator>FLETCHER, Alan</creator><creator>SALDANHA, Gerald S</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080915</creationdate><title>WNT5A Expression Increases during Melanoma Progression and Correlates with Outcome</title><author>DA FORNO, Philip D ; PRINGLE, J. Howard ; HUTCHINSON, Peter ; OSBORN, Joy ; QIANG HUANG ; POTTER, Linda ; HANCOX, Rachael A ; FLETCHER, Alan ; SALDANHA, Gerald S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-dec796ede4c7b9b38e3aa73450b34bcd4c5aea4dc67fe965582b05a29c7a11bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>Cyclin-Dependent Kinase Inhibitor p16 - metabolism</topic><topic>Cytoplasm - metabolism</topic><topic>Dermatology</topic><topic>Disease Progression</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>melanoma</topic><topic>Melanoma - metabolism</topic><topic>Melanoma - pathology</topic><topic>metastasis</topic><topic>Middle Aged</topic><topic>Neoplasm Metastasis</topic><topic>Pharmacology. Drug treatments</topic><topic>Prognosis</topic><topic>progression</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Skin Neoplasms - metabolism</topic><topic>Skin Neoplasms - pathology</topic><topic>survival</topic><topic>Survival Analysis</topic><topic>Tumors of the skin and soft tissue. Premalignant lesions</topic><topic>Wnt Proteins - metabolism</topic><topic>Wnt-5a Protein</topic><topic>WNT5A</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DA FORNO, Philip D</creatorcontrib><creatorcontrib>PRINGLE, J. Howard</creatorcontrib><creatorcontrib>HUTCHINSON, Peter</creatorcontrib><creatorcontrib>OSBORN, Joy</creatorcontrib><creatorcontrib>QIANG HUANG</creatorcontrib><creatorcontrib>POTTER, Linda</creatorcontrib><creatorcontrib>HANCOX, Rachael A</creatorcontrib><creatorcontrib>FLETCHER, Alan</creatorcontrib><creatorcontrib>SALDANHA, Gerald S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DA FORNO, Philip D</au><au>PRINGLE, J. Howard</au><au>HUTCHINSON, Peter</au><au>OSBORN, Joy</au><au>QIANG HUANG</au><au>POTTER, Linda</au><au>HANCOX, Rachael A</au><au>FLETCHER, Alan</au><au>SALDANHA, Gerald S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>WNT5A Expression Increases during Melanoma Progression and Correlates with Outcome</atitle><jtitle>Clinical cancer research</jtitle><addtitle>Clin Cancer Res</addtitle><date>2008-09-15</date><risdate>2008</risdate><volume>14</volume><issue>18</issue><spage>5825</spage><epage>5832</epage><pages>5825-5832</pages><issn>1078-0432</issn><eissn>1557-3265</eissn><coden>CCREF4</coden><abstract>Purpose: Wnt ligands play a major role in development and are important in cancer. Expression microarray analysis correlates one member
of this family, WNT5A, to a subclass of melanomas with increased motility and invasion. There are no large studies of clinical
samples primarily addressing the importance of WNT5A in melanoma progression or outcome. Therefore, this study aimed to assess
the protein expression of WNT5A during melanoma progression and its effect on outcome.
Experimental Design: Expression of WNT5A was determined in a series of 59 primary melanomas with matched metastases. To provide a benchmark of
progression against which to assess WNT5A, expression of p16 ink4a was analyzed, as this has been previously well documented in melanoma. The effect of WNT5A protein expression on outcome
was assessed in 102 melanomas.
Results: Cytoplasmic WNT5A showed a trend of increasing expression with melanoma progression ( P = 0.013), whereas there was diminishing p16 ink4a expression ( P = 0.006). Nevi showed relatively strong WNT5A expression. Strong cytoplasmic WNT5A was an independent risk factor for reduced
metastasis-free and overall survival in multivariate analysis ( P = 0.001 and 0.003, respectively).
Conclusion: Cytoplasmic WNT5A increases with melanoma progression and strong expression is associated with poor outcome.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>18794093</pmid><doi>10.1158/1078-0432.CCR-07-5104</doi><tpages>8</tpages></addata></record> |
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| ispartof | Clinical cancer research, 2008-09, Vol.14 (18), p.5825-5832 |
| issn | 1078-0432 1557-3265 |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Antineoplastic agents Biological and medical sciences Cell Line, Tumor Cyclin-Dependent Kinase Inhibitor p16 - metabolism Cytoplasm - metabolism Dermatology Disease Progression Female Humans Male Medical sciences melanoma Melanoma - metabolism Melanoma - pathology metastasis Middle Aged Neoplasm Metastasis Pharmacology. Drug treatments Prognosis progression Proto-Oncogene Proteins - metabolism Skin Neoplasms - metabolism Skin Neoplasms - pathology survival Survival Analysis Tumors of the skin and soft tissue. Premalignant lesions Wnt Proteins - metabolism Wnt-5a Protein WNT5A |
| title | WNT5A Expression Increases during Melanoma Progression and Correlates with Outcome |
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