Brain-derived neurotrophic factor suppresses anoikis-induced death of Schwann cells
Anoikis is a type of apoptosis due to the detachment from the extracellular matrix and neighboring cells. In case of cell transplantation therapy for spinal cord injury, preparation of graft cells includes dissociation of cultured cells, which may cause anoikis-induced cell death. Thus suppression o...
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| Veröffentlicht in: | Neuroscience letters 2008-10, Vol.444 (2), p.143-147 |
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| container_title | Neuroscience letters |
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| creator | Koda, Masao Someya, Yukio Nishio, Yutaka Kadota, Ryo Mannoji, Chikato Miyashita, Tomohiro Okawa, Akihiko Murata, Atsushi Yamazaki, Masashi |
| description | Anoikis is a type of apoptosis due to the detachment from the extracellular matrix and neighboring cells. In case of cell transplantation therapy for spinal cord injury, preparation of graft cells includes dissociation of cultured cells, which may cause anoikis-induced cell death. Thus suppression of anoikis may increase survival of grafted cells. Here we tested the effect of brain-derived neurotrophic factor (BDNF) on anoikis-induced cell death of cultured Schwann cells. Schwann cells were collected and cultured from sciatic nerves of neonatal Wistar rats. Schwann cells were plated upon a non-adherent polyhydroxyethyl methacrylate substrate to induce anoikis. BDNF was added into the culture medium at various concentrations. Twenty-four hours after non-adherent culture, approximately 40% of Schwann cells died and BDNF significantly decreased the number of dead cells in that culture condition. Next, Schwann cells were transplanted with or without BDNF treatment into contused rat spinal cord 1 week after injury. Five weeks after transplantation, immunohistochemistry revealed that the number of transplanted cells was significantly larger in the BDNF-treated group than that of the non-treated group. Suppression of anoikis may increase survival of grafted cells in case of cell therapy for spinal cord injury. |
| doi_str_mv | 10.1016/j.neulet.2008.07.055 |
| format | Article |
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In case of cell transplantation therapy for spinal cord injury, preparation of graft cells includes dissociation of cultured cells, which may cause anoikis-induced cell death. Thus suppression of anoikis may increase survival of grafted cells. Here we tested the effect of brain-derived neurotrophic factor (BDNF) on anoikis-induced cell death of cultured Schwann cells. Schwann cells were collected and cultured from sciatic nerves of neonatal Wistar rats. Schwann cells were plated upon a non-adherent polyhydroxyethyl methacrylate substrate to induce anoikis. BDNF was added into the culture medium at various concentrations. Twenty-four hours after non-adherent culture, approximately 40% of Schwann cells died and BDNF significantly decreased the number of dead cells in that culture condition. Next, Schwann cells were transplanted with or without BDNF treatment into contused rat spinal cord 1 week after injury. Five weeks after transplantation, immunohistochemistry revealed that the number of transplanted cells was significantly larger in the BDNF-treated group than that of the non-treated group. Suppression of anoikis may increase survival of grafted cells in case of cell therapy for spinal cord injury.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2008.07.055</identifier><identifier>PMID: 18672025</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Animals, Newborn ; Anoikis ; Biological and medical sciences ; Brain-Derived Neurotrophic Factor - pharmacology ; Cell therapy ; Cells, Cultured ; Female ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; Rats ; Rats, Wistar ; Schwann Cells - drug effects ; Schwann Cells - physiology ; Schwann Cells - transplantation ; Sciatic Nerve - cytology ; Spinal Cord Injuries - pathology ; Spinal Cord Injuries - therapy ; Spinal cord injury ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 2008-10, Vol.444 (2), p.143-147</ispartof><rights>2008 Elsevier Ireland Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-7371b8176f94943f646a0d1172748a9086d3b64acbe0efc961cb3c119c81c9f43</citedby><cites>FETCH-LOGICAL-c487t-7371b8176f94943f646a0d1172748a9086d3b64acbe0efc961cb3c119c81c9f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.neulet.2008.07.055$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20759523$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18672025$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koda, Masao</creatorcontrib><creatorcontrib>Someya, Yukio</creatorcontrib><creatorcontrib>Nishio, Yutaka</creatorcontrib><creatorcontrib>Kadota, Ryo</creatorcontrib><creatorcontrib>Mannoji, Chikato</creatorcontrib><creatorcontrib>Miyashita, Tomohiro</creatorcontrib><creatorcontrib>Okawa, Akihiko</creatorcontrib><creatorcontrib>Murata, Atsushi</creatorcontrib><creatorcontrib>Yamazaki, Masashi</creatorcontrib><title>Brain-derived neurotrophic factor suppresses anoikis-induced death of Schwann cells</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Anoikis is a type of apoptosis due to the detachment from the extracellular matrix and neighboring cells. In case of cell transplantation therapy for spinal cord injury, preparation of graft cells includes dissociation of cultured cells, which may cause anoikis-induced cell death. Thus suppression of anoikis may increase survival of grafted cells. Here we tested the effect of brain-derived neurotrophic factor (BDNF) on anoikis-induced cell death of cultured Schwann cells. Schwann cells were collected and cultured from sciatic nerves of neonatal Wistar rats. Schwann cells were plated upon a non-adherent polyhydroxyethyl methacrylate substrate to induce anoikis. BDNF was added into the culture medium at various concentrations. Twenty-four hours after non-adherent culture, approximately 40% of Schwann cells died and BDNF significantly decreased the number of dead cells in that culture condition. Next, Schwann cells were transplanted with or without BDNF treatment into contused rat spinal cord 1 week after injury. Five weeks after transplantation, immunohistochemistry revealed that the number of transplanted cells was significantly larger in the BDNF-treated group than that of the non-treated group. Suppression of anoikis may increase survival of grafted cells in case of cell therapy for spinal cord injury.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Anoikis</subject><subject>Biological and medical sciences</subject><subject>Brain-Derived Neurotrophic Factor - pharmacology</subject><subject>Cell therapy</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Schwann Cells - drug effects</subject><subject>Schwann Cells - physiology</subject><subject>Schwann Cells - transplantation</subject><subject>Sciatic Nerve - cytology</subject><subject>Spinal Cord Injuries - pathology</subject><subject>Spinal Cord Injuries - therapy</subject><subject>Spinal cord injury</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD2P1DAURS0EYoeFf4BQGugS3nPs2G6QYMWXtBLFQm05zrPGQyYOdrKIf09GM4IOqtuce3V1GHuO0CBg9_rQTLSOtDQcQDegGpDyAduhVrxWRvGHbActiLo1Aq7Yk1IOACBRisfsCnWnOHC5Y3fvsotTPVCO9zRU22ROS07zPvoqOL-kXJV1njOVQqVyU4rfY6njNKx-wwdyy75Kobrz-59umipP41ieskfBjYWeXfKaffvw_uvNp_r2y8fPN29vay-0WmrVKuw1qi4YYUQbOtE5GBAVV0I7A7ob2r4TzvcEFLzp0PetRzReozdBtNfs1Xl3zunHSmWxx1hOD9xEaS22M1Ii6va_IEfgRgrcQHEGfU6lZAp2zvHo8i-LYE_W7cGerduTdQvKbta32ovL_tofafhbumjegJcXwBXvxpDd5GP5w3FQ0kh-OvrmzNGm7T5StsVHmjbVMZNf7JDiv5_8BgmtokM</recordid><startdate>20081024</startdate><enddate>20081024</enddate><creator>Koda, Masao</creator><creator>Someya, Yukio</creator><creator>Nishio, Yutaka</creator><creator>Kadota, Ryo</creator><creator>Mannoji, Chikato</creator><creator>Miyashita, Tomohiro</creator><creator>Okawa, Akihiko</creator><creator>Murata, Atsushi</creator><creator>Yamazaki, Masashi</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20081024</creationdate><title>Brain-derived neurotrophic factor suppresses anoikis-induced death of Schwann cells</title><author>Koda, Masao ; Someya, Yukio ; Nishio, Yutaka ; Kadota, Ryo ; Mannoji, Chikato ; Miyashita, Tomohiro ; Okawa, Akihiko ; Murata, Atsushi ; Yamazaki, Masashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-7371b8176f94943f646a0d1172748a9086d3b64acbe0efc961cb3c119c81c9f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Anoikis</topic><topic>Biological and medical sciences</topic><topic>Brain-Derived Neurotrophic Factor - pharmacology</topic><topic>Cell therapy</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Schwann Cells - drug effects</topic><topic>Schwann Cells - physiology</topic><topic>Schwann Cells - transplantation</topic><topic>Sciatic Nerve - cytology</topic><topic>Spinal Cord Injuries - pathology</topic><topic>Spinal Cord Injuries - therapy</topic><topic>Spinal cord injury</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koda, Masao</creatorcontrib><creatorcontrib>Someya, Yukio</creatorcontrib><creatorcontrib>Nishio, Yutaka</creatorcontrib><creatorcontrib>Kadota, Ryo</creatorcontrib><creatorcontrib>Mannoji, Chikato</creatorcontrib><creatorcontrib>Miyashita, Tomohiro</creatorcontrib><creatorcontrib>Okawa, Akihiko</creatorcontrib><creatorcontrib>Murata, Atsushi</creatorcontrib><creatorcontrib>Yamazaki, Masashi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koda, Masao</au><au>Someya, Yukio</au><au>Nishio, Yutaka</au><au>Kadota, Ryo</au><au>Mannoji, Chikato</au><au>Miyashita, Tomohiro</au><au>Okawa, Akihiko</au><au>Murata, Atsushi</au><au>Yamazaki, Masashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Brain-derived neurotrophic factor suppresses anoikis-induced death of Schwann cells</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2008-10-24</date><risdate>2008</risdate><volume>444</volume><issue>2</issue><spage>143</spage><epage>147</epage><pages>143-147</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Anoikis is a type of apoptosis due to the detachment from the extracellular matrix and neighboring cells. In case of cell transplantation therapy for spinal cord injury, preparation of graft cells includes dissociation of cultured cells, which may cause anoikis-induced cell death. Thus suppression of anoikis may increase survival of grafted cells. Here we tested the effect of brain-derived neurotrophic factor (BDNF) on anoikis-induced cell death of cultured Schwann cells. Schwann cells were collected and cultured from sciatic nerves of neonatal Wistar rats. Schwann cells were plated upon a non-adherent polyhydroxyethyl methacrylate substrate to induce anoikis. BDNF was added into the culture medium at various concentrations. Twenty-four hours after non-adherent culture, approximately 40% of Schwann cells died and BDNF significantly decreased the number of dead cells in that culture condition. Next, Schwann cells were transplanted with or without BDNF treatment into contused rat spinal cord 1 week after injury. 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| ispartof | Neuroscience letters, 2008-10, Vol.444 (2), p.143-147 |
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| subjects | Animals Animals, Newborn Anoikis Biological and medical sciences Brain-Derived Neurotrophic Factor - pharmacology Cell therapy Cells, Cultured Female Fundamental and applied biological sciences. Psychology Immunohistochemistry Rats Rats, Wistar Schwann Cells - drug effects Schwann Cells - physiology Schwann Cells - transplantation Sciatic Nerve - cytology Spinal Cord Injuries - pathology Spinal Cord Injuries - therapy Spinal cord injury Vertebrates: nervous system and sense organs |
| title | Brain-derived neurotrophic factor suppresses anoikis-induced death of Schwann cells |
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