Administration of PLK-1 small interfering RNA with atelocollagen prevents the growth of liver metastases of lung cancer
Liver metastasis is one of the most important prognostic factors in lung cancer patients. However, current therapies are not sufficient. RNA interference provides us a powerful and promising approach for treating human diseases including cancers. Herein, we investigated the in vitro effects of PLK-1...
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| Veröffentlicht in: | Molecular cancer therapeutics 2008-09, Vol.7 (9), p.2904-2912 |
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| creator | Kawata, Eri Ashihara, Eishi Kimura, Shinya Takenaka, Kazumasa Sato, Kiyoshi Tanaka, Ruriko Yokota, Asumi Kamitsuji, Yuri Takeuchi, Miki Kuroda, Junya Tanaka, Fumihiro Yoshikawa, Toshikazu Maekawa, Taira |
| description | Liver metastasis is one of the most important prognostic factors in lung cancer patients. However, current therapies are not
sufficient. RNA interference provides us a powerful and promising approach for treating human diseases including cancers.
Herein, we investigated the in vitro effects of PLK-1 small interfering RNA (siRNA) on human lung cancer cell lines and the in vivo usage of PLK-1 siRNA with atelocollagen as a drug delivery system in a murine liver metastasis model of lung cancer. PLK-1
was overexpressed in cell lines and in cancerous tissues from lung cancer patients. PLK-1 siRNA treatment inhibited growth
and induced apoptosis in a concentration-dependent manner. To verify in vivo efficacy, we confirmed that atelocollagen was a useful drug delivery system in our model of implanted luciferase-labeled
A549 LUC cells by detecting reduced bioluminescence after an i.v. injection of luciferase GL3 siRNA/atelocollagen. PLK-1 siRNA/atelocollagen
was also successfully transfected into cells and inhibited the progression of metastases. This study shows the efficacy of
i.v. administration of PLK-1 siRNA/atelocollagen for liver metastases of lung cancer. We believe siRNA therapy will be a powerful
and promising strategy against advanced lung cancer. [Mol Cancer Ther 2008;7(9):2904–12] |
| doi_str_mv | 10.1158/1535-7163.MCT-08-0473 |
| format | Article |
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sufficient. RNA interference provides us a powerful and promising approach for treating human diseases including cancers.
Herein, we investigated the in vitro effects of PLK-1 small interfering RNA (siRNA) on human lung cancer cell lines and the in vivo usage of PLK-1 siRNA with atelocollagen as a drug delivery system in a murine liver metastasis model of lung cancer. PLK-1
was overexpressed in cell lines and in cancerous tissues from lung cancer patients. PLK-1 siRNA treatment inhibited growth
and induced apoptosis in a concentration-dependent manner. To verify in vivo efficacy, we confirmed that atelocollagen was a useful drug delivery system in our model of implanted luciferase-labeled
A549 LUC cells by detecting reduced bioluminescence after an i.v. injection of luciferase GL3 siRNA/atelocollagen. PLK-1 siRNA/atelocollagen
was also successfully transfected into cells and inhibited the progression of metastases. This study shows the efficacy of
i.v. administration of PLK-1 siRNA/atelocollagen for liver metastases of lung cancer. We believe siRNA therapy will be a powerful
and promising strategy against advanced lung cancer. [Mol Cancer Ther 2008;7(9):2904–12]</description><identifier>ISSN: 1535-7163</identifier><identifier>EISSN: 1538-8514</identifier><identifier>DOI: 10.1158/1535-7163.MCT-08-0473</identifier><identifier>PMID: 18790771</identifier><language>eng</language><publisher>United States: American Association for Cancer Research</publisher><subject><![CDATA[Aged ; Animals ; Cell Cycle Proteins - antagonists & inhibitors ; Cell Cycle Proteins - metabolism ; Cell Death - drug effects ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Collagen - administration & dosage ; Collagen - pharmacology ; Female ; Humans ; liver metastasis ; Liver Neoplasms - prevention & control ; Liver Neoplasms - secondary ; Lung Neoplasms - enzymology ; Lung Neoplasms - pathology ; Lung Neoplasms - ultrastructure ; Male ; Mice ; Mice, Nude ; Middle Aged ; PLK-1 ; Polo-Like Kinase 1 ; Protein Serine-Threonine Kinases - antagonists & inhibitors ; Protein Serine-Threonine Kinases - metabolism ; Proto-Oncogene Proteins - antagonists & inhibitors ; Proto-Oncogene Proteins - metabolism ; RNA, Small Interfering - administration & dosage ; RNA, Small Interfering - pharmacology ; siRNA ; Xenograft Model Antitumor Assays]]></subject><ispartof>Molecular cancer therapeutics, 2008-09, Vol.7 (9), p.2904-2912</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-ef9556eb3e949063860ed15ca03995a7b95c1a889729a251ca21972f6f9ed0bb3</citedby><cites>FETCH-LOGICAL-c386t-ef9556eb3e949063860ed15ca03995a7b95c1a889729a251ca21972f6f9ed0bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3343,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18790771$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawata, Eri</creatorcontrib><creatorcontrib>Ashihara, Eishi</creatorcontrib><creatorcontrib>Kimura, Shinya</creatorcontrib><creatorcontrib>Takenaka, Kazumasa</creatorcontrib><creatorcontrib>Sato, Kiyoshi</creatorcontrib><creatorcontrib>Tanaka, Ruriko</creatorcontrib><creatorcontrib>Yokota, Asumi</creatorcontrib><creatorcontrib>Kamitsuji, Yuri</creatorcontrib><creatorcontrib>Takeuchi, Miki</creatorcontrib><creatorcontrib>Kuroda, Junya</creatorcontrib><creatorcontrib>Tanaka, Fumihiro</creatorcontrib><creatorcontrib>Yoshikawa, Toshikazu</creatorcontrib><creatorcontrib>Maekawa, Taira</creatorcontrib><title>Administration of PLK-1 small interfering RNA with atelocollagen prevents the growth of liver metastases of lung cancer</title><title>Molecular cancer therapeutics</title><addtitle>Mol Cancer Ther</addtitle><description>Liver metastasis is one of the most important prognostic factors in lung cancer patients. However, current therapies are not
sufficient. RNA interference provides us a powerful and promising approach for treating human diseases including cancers.
Herein, we investigated the in vitro effects of PLK-1 small interfering RNA (siRNA) on human lung cancer cell lines and the in vivo usage of PLK-1 siRNA with atelocollagen as a drug delivery system in a murine liver metastasis model of lung cancer. PLK-1
was overexpressed in cell lines and in cancerous tissues from lung cancer patients. PLK-1 siRNA treatment inhibited growth
and induced apoptosis in a concentration-dependent manner. To verify in vivo efficacy, we confirmed that atelocollagen was a useful drug delivery system in our model of implanted luciferase-labeled
A549 LUC cells by detecting reduced bioluminescence after an i.v. injection of luciferase GL3 siRNA/atelocollagen. PLK-1 siRNA/atelocollagen
was also successfully transfected into cells and inhibited the progression of metastases. This study shows the efficacy of
i.v. administration of PLK-1 siRNA/atelocollagen for liver metastases of lung cancer. We believe siRNA therapy will be a powerful
and promising strategy against advanced lung cancer. [Mol Cancer Ther 2008;7(9):2904–12]</description><subject>Aged</subject><subject>Animals</subject><subject>Cell Cycle Proteins - antagonists & inhibitors</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cell Death - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Collagen - administration & dosage</subject><subject>Collagen - pharmacology</subject><subject>Female</subject><subject>Humans</subject><subject>liver metastasis</subject><subject>Liver Neoplasms - prevention & control</subject><subject>Liver Neoplasms - secondary</subject><subject>Lung Neoplasms - enzymology</subject><subject>Lung Neoplasms - pathology</subject><subject>Lung Neoplasms - ultrastructure</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Middle Aged</subject><subject>PLK-1</subject><subject>Polo-Like Kinase 1</subject><subject>Protein Serine-Threonine Kinases - antagonists & inhibitors</subject><subject>Protein Serine-Threonine Kinases - metabolism</subject><subject>Proto-Oncogene Proteins - antagonists & inhibitors</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>RNA, Small Interfering - administration & dosage</subject><subject>RNA, Small Interfering - pharmacology</subject><subject>siRNA</subject><subject>Xenograft Model Antitumor Assays</subject><issn>1535-7163</issn><issn>1538-8514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkFtLwzAYhoMoHqY_QcmVXnUmTdMml2N4wnlA9Dqk2dc10sNMUof_3swOhEA-Xp7vTXgQOqdkSikX15QznhQ0Z9On-XtCREKygu2h45iLRHCa7f_NI3OETrz_JIQKmdJDdERFIUlR0GO0mS1b21kfnA6273Bf4dfFY0Kxb3XTYNsFcBU4263w2_MMb2yosQ7Q9KZvGr2CDq8dfEMXPA414JXrN5GILY39BodbCNrHA_4vG2KN0Z0Bd4oOKt14ONvdE_Rxe_M-v08WL3cP89kiMUzkIYFKcp5DyUBmkuQxI7Ck3GjCpOS6KCU3VAshi1TqlFOjUxrnKq8kLElZsgm6HHvXrv8awAfVWm8g_r2DfvAqlzwTTIoI8hE0rvfeQaXWzrba_ShK1Na42tpUW5sqGldEqK3xuHexe2AoW1j-b-0UR-BqBGq7qjfWgRoNOPCgnalVoaRKJcnYL-t-i50</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Kawata, Eri</creator><creator>Ashihara, Eishi</creator><creator>Kimura, Shinya</creator><creator>Takenaka, Kazumasa</creator><creator>Sato, Kiyoshi</creator><creator>Tanaka, Ruriko</creator><creator>Yokota, Asumi</creator><creator>Kamitsuji, Yuri</creator><creator>Takeuchi, Miki</creator><creator>Kuroda, Junya</creator><creator>Tanaka, Fumihiro</creator><creator>Yoshikawa, Toshikazu</creator><creator>Maekawa, Taira</creator><general>American Association for Cancer Research</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>Administration of PLK-1 small interfering RNA with atelocollagen prevents the growth of liver metastases of lung cancer</title><author>Kawata, Eri ; Ashihara, Eishi ; Kimura, Shinya ; Takenaka, Kazumasa ; Sato, Kiyoshi ; Tanaka, Ruriko ; Yokota, Asumi ; Kamitsuji, Yuri ; Takeuchi, Miki ; Kuroda, Junya ; Tanaka, Fumihiro ; Yoshikawa, Toshikazu ; Maekawa, Taira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-ef9556eb3e949063860ed15ca03995a7b95c1a889729a251ca21972f6f9ed0bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Aged</topic><topic>Animals</topic><topic>Cell Cycle Proteins - antagonists & inhibitors</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Cell Death - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Collagen - administration & dosage</topic><topic>Collagen - pharmacology</topic><topic>Female</topic><topic>Humans</topic><topic>liver metastasis</topic><topic>Liver Neoplasms - prevention & control</topic><topic>Liver Neoplasms - secondary</topic><topic>Lung Neoplasms - enzymology</topic><topic>Lung Neoplasms - pathology</topic><topic>Lung Neoplasms - ultrastructure</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Middle Aged</topic><topic>PLK-1</topic><topic>Polo-Like Kinase 1</topic><topic>Protein Serine-Threonine Kinases - antagonists & inhibitors</topic><topic>Protein Serine-Threonine Kinases - metabolism</topic><topic>Proto-Oncogene Proteins - antagonists & inhibitors</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>RNA, Small Interfering - administration & dosage</topic><topic>RNA, Small Interfering - pharmacology</topic><topic>siRNA</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawata, Eri</creatorcontrib><creatorcontrib>Ashihara, Eishi</creatorcontrib><creatorcontrib>Kimura, Shinya</creatorcontrib><creatorcontrib>Takenaka, Kazumasa</creatorcontrib><creatorcontrib>Sato, Kiyoshi</creatorcontrib><creatorcontrib>Tanaka, Ruriko</creatorcontrib><creatorcontrib>Yokota, Asumi</creatorcontrib><creatorcontrib>Kamitsuji, Yuri</creatorcontrib><creatorcontrib>Takeuchi, Miki</creatorcontrib><creatorcontrib>Kuroda, Junya</creatorcontrib><creatorcontrib>Tanaka, Fumihiro</creatorcontrib><creatorcontrib>Yoshikawa, Toshikazu</creatorcontrib><creatorcontrib>Maekawa, Taira</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular cancer therapeutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawata, Eri</au><au>Ashihara, Eishi</au><au>Kimura, Shinya</au><au>Takenaka, Kazumasa</au><au>Sato, Kiyoshi</au><au>Tanaka, Ruriko</au><au>Yokota, Asumi</au><au>Kamitsuji, Yuri</au><au>Takeuchi, Miki</au><au>Kuroda, Junya</au><au>Tanaka, Fumihiro</au><au>Yoshikawa, Toshikazu</au><au>Maekawa, Taira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Administration of PLK-1 small interfering RNA with atelocollagen prevents the growth of liver metastases of lung cancer</atitle><jtitle>Molecular cancer therapeutics</jtitle><addtitle>Mol Cancer Ther</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>7</volume><issue>9</issue><spage>2904</spage><epage>2912</epage><pages>2904-2912</pages><issn>1535-7163</issn><eissn>1538-8514</eissn><abstract>Liver metastasis is one of the most important prognostic factors in lung cancer patients. However, current therapies are not
sufficient. RNA interference provides us a powerful and promising approach for treating human diseases including cancers.
Herein, we investigated the in vitro effects of PLK-1 small interfering RNA (siRNA) on human lung cancer cell lines and the in vivo usage of PLK-1 siRNA with atelocollagen as a drug delivery system in a murine liver metastasis model of lung cancer. PLK-1
was overexpressed in cell lines and in cancerous tissues from lung cancer patients. PLK-1 siRNA treatment inhibited growth
and induced apoptosis in a concentration-dependent manner. To verify in vivo efficacy, we confirmed that atelocollagen was a useful drug delivery system in our model of implanted luciferase-labeled
A549 LUC cells by detecting reduced bioluminescence after an i.v. injection of luciferase GL3 siRNA/atelocollagen. PLK-1 siRNA/atelocollagen
was also successfully transfected into cells and inhibited the progression of metastases. This study shows the efficacy of
i.v. administration of PLK-1 siRNA/atelocollagen for liver metastases of lung cancer. We believe siRNA therapy will be a powerful
and promising strategy against advanced lung cancer. [Mol Cancer Ther 2008;7(9):2904–12]</abstract><cop>United States</cop><pub>American Association for Cancer Research</pub><pmid>18790771</pmid><doi>10.1158/1535-7163.MCT-08-0473</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals |
| subjects | Aged Animals Cell Cycle Proteins - antagonists & inhibitors Cell Cycle Proteins - metabolism Cell Death - drug effects Cell Line, Tumor Cell Proliferation - drug effects Collagen - administration & dosage Collagen - pharmacology Female Humans liver metastasis Liver Neoplasms - prevention & control Liver Neoplasms - secondary Lung Neoplasms - enzymology Lung Neoplasms - pathology Lung Neoplasms - ultrastructure Male Mice Mice, Nude Middle Aged PLK-1 Polo-Like Kinase 1 Protein Serine-Threonine Kinases - antagonists & inhibitors Protein Serine-Threonine Kinases - metabolism Proto-Oncogene Proteins - antagonists & inhibitors Proto-Oncogene Proteins - metabolism RNA, Small Interfering - administration & dosage RNA, Small Interfering - pharmacology siRNA Xenograft Model Antitumor Assays |
| title | Administration of PLK-1 small interfering RNA with atelocollagen prevents the growth of liver metastases of lung cancer |
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