Effect of inhaled glucocorticoid on the cellular profile and cytokine levels in induced sputum from asthmatic patients
Cytokines are considered to play a role in the airway inflammation of bronchial asthma. We examined the cellular profile and cytokine levels in induced sputum samples obtained before and after treatment with beclomethasone dipropionate (BDP, 800 microg/day, for 4 weeks) in 12 mild to moderate asthma...
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| Veröffentlicht in: | Lung 1999, Vol.177 (1), p.53-62 |
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| creator | Inoue, H Aizawa, H Fukuyama, S Takata, S Matsumoto, K Shigyo, M Koto, H Hara, N |
| description | Cytokines are considered to play a role in the airway inflammation of bronchial asthma. We examined the cellular profile and cytokine levels in induced sputum samples obtained before and after treatment with beclomethasone dipropionate (BDP, 800 microg/day, for 4 weeks) in 12 mild to moderate asthmatic subjects who had not previously received inhaled glucocorticosteroids. Sputum was induced with a 20-min inhalation of 3% saline by an ultrasonic nebulizer. The freshly expectorated sputum separated from the saliva was analyzed for cell counts, for the concentration of interleukin-8 (IL-8), and for the concentration of granulocyte-macrophage colony-stimulating factor (GM-CSF). The mean percentage of eosinophils in the sputum samples decreased significantly after BDP treatment, but no significant change in the percentage of neutrophils was observed. The mean IL-8 and GM-CSF levels also decreased significantly after treatment. The BDP treatment was associated with an increase in the mean peak expiratory flow (PEF) and with a decrease in the diurnal variation of PEF. These results suggest that inhaled steroids improve airway inflammation and lung function in asthmatics, presumably in part by inhibiting the synthesis of inflammatory cytokines such as IL-8 and GM-CSF. |
| doi_str_mv | 10.1007/pl00007627 |
| format | Article |
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We examined the cellular profile and cytokine levels in induced sputum samples obtained before and after treatment with beclomethasone dipropionate (BDP, 800 microg/day, for 4 weeks) in 12 mild to moderate asthmatic subjects who had not previously received inhaled glucocorticosteroids. Sputum was induced with a 20-min inhalation of 3% saline by an ultrasonic nebulizer. The freshly expectorated sputum separated from the saliva was analyzed for cell counts, for the concentration of interleukin-8 (IL-8), and for the concentration of granulocyte-macrophage colony-stimulating factor (GM-CSF). The mean percentage of eosinophils in the sputum samples decreased significantly after BDP treatment, but no significant change in the percentage of neutrophils was observed. The mean IL-8 and GM-CSF levels also decreased significantly after treatment. The BDP treatment was associated with an increase in the mean peak expiratory flow (PEF) and with a decrease in the diurnal variation of PEF. These results suggest that inhaled steroids improve airway inflammation and lung function in asthmatics, presumably in part by inhibiting the synthesis of inflammatory cytokines such as IL-8 and GM-CSF.</description><identifier>ISSN: 0341-2040</identifier><identifier>EISSN: 1432-1750</identifier><identifier>DOI: 10.1007/pl00007627</identifier><identifier>PMID: 9835634</identifier><identifier>CODEN: LUNGD9</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Administration, Inhalation ; Administration, Topical ; Anti-Asthmatic Agents - therapeutic use ; Anti-Inflammatory Agents - therapeutic use ; Asthma - diagnosis ; Asthma - drug therapy ; Asthma - metabolism ; Beclomethasone - therapeutic use ; Cell Count ; Eosinophils ; Female ; Glucocorticoids ; Granulocyte-Macrophage Colony-Stimulating Factor - metabolism ; Humans ; Interleukin-8 - metabolism ; Male ; Middle Aged ; Neutrophils ; Peak Expiratory Flow Rate ; Reproducibility of Results ; Spirometry ; Sputum - chemistry ; Sputum - cytology</subject><ispartof>Lung, 1999, Vol.177 (1), p.53-62</ispartof><rights>Springer-Verlag New York Inc. 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-e7e359b44e50d00d721b30730cd8afd71a09d1e81513624b6fbfec6fc39eab863</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9835634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inoue, H</creatorcontrib><creatorcontrib>Aizawa, H</creatorcontrib><creatorcontrib>Fukuyama, S</creatorcontrib><creatorcontrib>Takata, S</creatorcontrib><creatorcontrib>Matsumoto, K</creatorcontrib><creatorcontrib>Shigyo, M</creatorcontrib><creatorcontrib>Koto, H</creatorcontrib><creatorcontrib>Hara, N</creatorcontrib><title>Effect of inhaled glucocorticoid on the cellular profile and cytokine levels in induced sputum from asthmatic patients</title><title>Lung</title><addtitle>Lung</addtitle><description>Cytokines are considered to play a role in the airway inflammation of bronchial asthma. We examined the cellular profile and cytokine levels in induced sputum samples obtained before and after treatment with beclomethasone dipropionate (BDP, 800 microg/day, for 4 weeks) in 12 mild to moderate asthmatic subjects who had not previously received inhaled glucocorticosteroids. Sputum was induced with a 20-min inhalation of 3% saline by an ultrasonic nebulizer. The freshly expectorated sputum separated from the saliva was analyzed for cell counts, for the concentration of interleukin-8 (IL-8), and for the concentration of granulocyte-macrophage colony-stimulating factor (GM-CSF). The mean percentage of eosinophils in the sputum samples decreased significantly after BDP treatment, but no significant change in the percentage of neutrophils was observed. The mean IL-8 and GM-CSF levels also decreased significantly after treatment. The BDP treatment was associated with an increase in the mean peak expiratory flow (PEF) and with a decrease in the diurnal variation of PEF. 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therapeutic use</topic><topic>Anti-Inflammatory Agents - therapeutic use</topic><topic>Asthma - diagnosis</topic><topic>Asthma - drug therapy</topic><topic>Asthma - metabolism</topic><topic>Beclomethasone - therapeutic use</topic><topic>Cell Count</topic><topic>Eosinophils</topic><topic>Female</topic><topic>Glucocorticoids</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</topic><topic>Humans</topic><topic>Interleukin-8 - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neutrophils</topic><topic>Peak Expiratory Flow Rate</topic><topic>Reproducibility of Results</topic><topic>Spirometry</topic><topic>Sputum - chemistry</topic><topic>Sputum - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inoue, H</creatorcontrib><creatorcontrib>Aizawa, H</creatorcontrib><creatorcontrib>Fukuyama, S</creatorcontrib><creatorcontrib>Takata, S</creatorcontrib><creatorcontrib>Matsumoto, K</creatorcontrib><creatorcontrib>Shigyo, M</creatorcontrib><creatorcontrib>Koto, H</creatorcontrib><creatorcontrib>Hara, N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Lung</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inoue, H</au><au>Aizawa, H</au><au>Fukuyama, S</au><au>Takata, S</au><au>Matsumoto, K</au><au>Shigyo, M</au><au>Koto, H</au><au>Hara, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of inhaled glucocorticoid on the cellular profile and cytokine levels in induced sputum from asthmatic patients</atitle><jtitle>Lung</jtitle><addtitle>Lung</addtitle><date>1999</date><risdate>1999</risdate><volume>177</volume><issue>1</issue><spage>53</spage><epage>62</epage><pages>53-62</pages><issn>0341-2040</issn><eissn>1432-1750</eissn><coden>LUNGD9</coden><abstract>Cytokines are considered to play a role in the airway inflammation of bronchial asthma. We examined the cellular profile and cytokine levels in induced sputum samples obtained before and after treatment with beclomethasone dipropionate (BDP, 800 microg/day, for 4 weeks) in 12 mild to moderate asthmatic subjects who had not previously received inhaled glucocorticosteroids. Sputum was induced with a 20-min inhalation of 3% saline by an ultrasonic nebulizer. The freshly expectorated sputum separated from the saliva was analyzed for cell counts, for the concentration of interleukin-8 (IL-8), and for the concentration of granulocyte-macrophage colony-stimulating factor (GM-CSF). The mean percentage of eosinophils in the sputum samples decreased significantly after BDP treatment, but no significant change in the percentage of neutrophils was observed. The mean IL-8 and GM-CSF levels also decreased significantly after treatment. The BDP treatment was associated with an increase in the mean peak expiratory flow (PEF) and with a decrease in the diurnal variation of PEF. These results suggest that inhaled steroids improve airway inflammation and lung function in asthmatics, presumably in part by inhibiting the synthesis of inflammatory cytokines such as IL-8 and GM-CSF.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>9835634</pmid><doi>10.1007/pl00007627</doi><tpages>10</tpages></addata></record> |
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| subjects | Administration, Inhalation Administration, Topical Anti-Asthmatic Agents - therapeutic use Anti-Inflammatory Agents - therapeutic use Asthma - diagnosis Asthma - drug therapy Asthma - metabolism Beclomethasone - therapeutic use Cell Count Eosinophils Female Glucocorticoids Granulocyte-Macrophage Colony-Stimulating Factor - metabolism Humans Interleukin-8 - metabolism Male Middle Aged Neutrophils Peak Expiratory Flow Rate Reproducibility of Results Spirometry Sputum - chemistry Sputum - cytology |
| title | Effect of inhaled glucocorticoid on the cellular profile and cytokine levels in induced sputum from asthmatic patients |
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