Non-O157:H7 Pathogenic Shiga Toxin-Producing Escherichia coli: Phenotypic and Genetic Profiling of Virulence Traits and Evidence for Clonality
The virulence profiles of most non-O157 Shiga toxin (Stx)—producing Escherichia coli (STEC) are unknown. STEC belonging to serogroups O26,O103, and O111 were characterized to determine clonality and to profile virulence traits. Isolates within STEC serogroups O26, O111, and O103 were > 80% identi...
Gespeichert in:
| Veröffentlicht in: | The Journal of infectious diseases 1999-01, Vol.179 (1), p.115-123 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 123 |
|---|---|
| container_issue | 1 |
| container_start_page | 115 |
| container_title | The Journal of infectious diseases |
| container_volume | 179 |
| creator | Schmidt, Herbert Geitz, Caren Tarr, Phillip I. Frosch, Matthias Karch, Helge |
| description | The virulence profiles of most non-O157 Shiga toxin (Stx)—producing Escherichia coli (STEC) are unknown. STEC belonging to serogroups O26,O103, and O111 were characterized to determine clonality and to profile virulence traits. Isolates within STEC serogroups O26, O111, and O103 were > 80% identical in their randomly amplified polymorphic DNA pattern, suggesting clonality in these serogroups. The serogroups were distantly related to each other and to E. coli O157:H7. stx restriction fragment length polymorphism differentiated some STEC with the same randomly amplified polymorphic DNA pattern. The hemolytic phenotype of STEC O103:H2 isolates resembled that of α- and not enterohemorrhagic E. coli hemolysin. Virulence gene (eae, E-hly, espP, etp, katP, stx) probing demonstrated serogroup-specific profiles. Pathogenic STEC O26, O103, and O111 belong to their own lineages and have unique profiles of virulence traits that are different from the virulence profile of E. coli O157:H7. |
| doi_str_mv | 10.1086/314537 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_69547433</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30117234</jstor_id><sourcerecordid>30117234</sourcerecordid><originalsourceid>FETCH-LOGICAL-c390t-592414026c90c53fef2d6c8e36b614fb3d450578920a4607e26451dfd33ee7b93</originalsourceid><addsrcrecordid>eNpdkV-LEzEUxQdR1u6q30AIIr6N5t8kk31banerFrdgFfElpJmkkzpNajKzbL-En9nstrTg073c3zmHC6coXiH4HsGafSCIVoQ_KUYoj5IxRJ4WIwgxLlEtxPPiPKU1hJASxs-KM1FTVBM4Kv5-Db68RRW_nHIwV30bVsY7Db61bqXAItw7X85jaAbt_ApMkm5NdLp1CujQuUswb40P_W6bLco34MZ40-c9W6zrHizBgh8uDp3x2oBFVK5Pj8rJnWsebzZEMO6CV53rdy-KZ1Z1ybw8zIvi-_VkMZ6Ws9ubT-OrWamJgH1ZCUwRhZhpAXVFrLG4Ybo2hC0ZonZJGlrBitcCQ0UZ5AYzWqHGNoQYw5eCXBTv9rnbGP4MJvVy45I2Xae8CUOSTFSUU0Ky8M1_wnUYYn42SYzzLwIzfErTMaQUjZXb6DYq7iSC8qEduW8nC18f0oblxjRH2aGOzN8euEpadTYqr106pTFS5XpPMevUh3jEBCLEMaGZl3vuUm_uj1zF35Jxwis5_flL8tmX6WfBruVH8g9aDa0n</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>223909262</pqid></control><display><type>article</type><title>Non-O157:H7 Pathogenic Shiga Toxin-Producing Escherichia coli: Phenotypic and Genetic Profiling of Virulence Traits and Evidence for Clonality</title><source>MEDLINE</source><source>Jstor Complete Legacy</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Schmidt, Herbert ; Geitz, Caren ; Tarr, Phillip I. ; Frosch, Matthias ; Karch, Helge</creator><creatorcontrib>Schmidt, Herbert ; Geitz, Caren ; Tarr, Phillip I. ; Frosch, Matthias ; Karch, Helge</creatorcontrib><description>The virulence profiles of most non-O157 Shiga toxin (Stx)—producing Escherichia coli (STEC) are unknown. STEC belonging to serogroups O26,O103, and O111 were characterized to determine clonality and to profile virulence traits. Isolates within STEC serogroups O26, O111, and O103 were > 80% identical in their randomly amplified polymorphic DNA pattern, suggesting clonality in these serogroups. The serogroups were distantly related to each other and to E. coli O157:H7. stx restriction fragment length polymorphism differentiated some STEC with the same randomly amplified polymorphic DNA pattern. The hemolytic phenotype of STEC O103:H2 isolates resembled that of α- and not enterohemorrhagic E. coli hemolysin. Virulence gene (eae, E-hly, espP, etp, katP, stx) probing demonstrated serogroup-specific profiles. Pathogenic STEC O26, O103, and O111 belong to their own lineages and have unique profiles of virulence traits that are different from the virulence profile of E. coli O157:H7.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/314537</identifier><identifier>PMID: 9841830</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: University Chicago Press</publisher><subject>Bacterial Toxins - biosynthesis ; Bacterial Toxins - genetics ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Diarrhea - microbiology ; DNA ; DNA Primers - genetics ; Escherichia coli ; Escherichia coli - classification ; Escherichia coli - genetics ; Escherichia coli - pathogenicity ; Escherichia coli Infections - microbiology ; Escherichia coli O157 ; Escherichia coli O157 - classification ; Escherichia coli O157 - genetics ; Escherichia coli O157 - pathogenicity ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetic Markers ; Hemolysin proteins ; Hemolysis ; Hemolytic uremic syndrome ; Humans ; Major Articles ; Microbiology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Phenotype ; Phenotypes ; Plasmids ; Plasmids - genetics ; Polymerase chain reaction ; Polymorphism, Restriction Fragment Length ; Random Amplified Polymorphic DNA Technique ; Serotyping ; Shiga Toxins ; Virulence ; Virulence - genetics</subject><ispartof>The Journal of infectious diseases, 1999-01, Vol.179 (1), p.115-123</ispartof><rights>Copyright 1999 Infectious Diseases Society of America</rights><rights>1999 INIST-CNRS</rights><rights>Copyright University of Chicago, acting through its Press Jan 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-592414026c90c53fef2d6c8e36b614fb3d450578920a4607e26451dfd33ee7b93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30117234$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30117234$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,778,782,801,4012,27910,27911,27912,58004,58237</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1635153$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9841830$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schmidt, Herbert</creatorcontrib><creatorcontrib>Geitz, Caren</creatorcontrib><creatorcontrib>Tarr, Phillip I.</creatorcontrib><creatorcontrib>Frosch, Matthias</creatorcontrib><creatorcontrib>Karch, Helge</creatorcontrib><title>Non-O157:H7 Pathogenic Shiga Toxin-Producing Escherichia coli: Phenotypic and Genetic Profiling of Virulence Traits and Evidence for Clonality</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><description>The virulence profiles of most non-O157 Shiga toxin (Stx)—producing Escherichia coli (STEC) are unknown. STEC belonging to serogroups O26,O103, and O111 were characterized to determine clonality and to profile virulence traits. Isolates within STEC serogroups O26, O111, and O103 were > 80% identical in their randomly amplified polymorphic DNA pattern, suggesting clonality in these serogroups. The serogroups were distantly related to each other and to E. coli O157:H7. stx restriction fragment length polymorphism differentiated some STEC with the same randomly amplified polymorphic DNA pattern. The hemolytic phenotype of STEC O103:H2 isolates resembled that of α- and not enterohemorrhagic E. coli hemolysin. Virulence gene (eae, E-hly, espP, etp, katP, stx) probing demonstrated serogroup-specific profiles. Pathogenic STEC O26, O103, and O111 belong to their own lineages and have unique profiles of virulence traits that are different from the virulence profile of E. coli O157:H7.</description><subject>Bacterial Toxins - biosynthesis</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Diarrhea - microbiology</subject><subject>DNA</subject><subject>DNA Primers - genetics</subject><subject>Escherichia coli</subject><subject>Escherichia coli - classification</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - pathogenicity</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli O157</subject><subject>Escherichia coli O157 - classification</subject><subject>Escherichia coli O157 - genetics</subject><subject>Escherichia coli O157 - pathogenicity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetic Markers</subject><subject>Hemolysin proteins</subject><subject>Hemolysis</subject><subject>Hemolytic uremic syndrome</subject><subject>Humans</subject><subject>Major Articles</subject><subject>Microbiology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>Plasmids</subject><subject>Plasmids - genetics</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>Serotyping</subject><subject>Shiga Toxins</subject><subject>Virulence</subject><subject>Virulence - genetics</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkV-LEzEUxQdR1u6q30AIIr6N5t8kk31banerFrdgFfElpJmkkzpNajKzbL-En9nstrTg073c3zmHC6coXiH4HsGafSCIVoQ_KUYoj5IxRJ4WIwgxLlEtxPPiPKU1hJASxs-KM1FTVBM4Kv5-Db68RRW_nHIwV30bVsY7Db61bqXAItw7X85jaAbt_ApMkm5NdLp1CujQuUswb40P_W6bLco34MZ40-c9W6zrHizBgh8uDp3x2oBFVK5Pj8rJnWsebzZEMO6CV53rdy-KZ1Z1ybw8zIvi-_VkMZ6Ws9ubT-OrWamJgH1ZCUwRhZhpAXVFrLG4Ybo2hC0ZonZJGlrBitcCQ0UZ5AYzWqHGNoQYw5eCXBTv9rnbGP4MJvVy45I2Xae8CUOSTFSUU0Ky8M1_wnUYYn42SYzzLwIzfErTMaQUjZXb6DYq7iSC8qEduW8nC18f0oblxjRH2aGOzN8euEpadTYqr106pTFS5XpPMevUh3jEBCLEMaGZl3vuUm_uj1zF35Jxwis5_flL8tmX6WfBruVH8g9aDa0n</recordid><startdate>199901</startdate><enddate>199901</enddate><creator>Schmidt, Herbert</creator><creator>Geitz, Caren</creator><creator>Tarr, Phillip I.</creator><creator>Frosch, Matthias</creator><creator>Karch, Helge</creator><general>University Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>199901</creationdate><title>Non-O157:H7 Pathogenic Shiga Toxin-Producing Escherichia coli: Phenotypic and Genetic Profiling of Virulence Traits and Evidence for Clonality</title><author>Schmidt, Herbert ; Geitz, Caren ; Tarr, Phillip I. ; Frosch, Matthias ; Karch, Helge</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-592414026c90c53fef2d6c8e36b614fb3d450578920a4607e26451dfd33ee7b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Bacterial Toxins - biosynthesis</topic><topic>Bacterial Toxins - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Diarrhea - microbiology</topic><topic>DNA</topic><topic>DNA Primers - genetics</topic><topic>Escherichia coli</topic><topic>Escherichia coli - classification</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - pathogenicity</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli O157</topic><topic>Escherichia coli O157 - classification</topic><topic>Escherichia coli O157 - genetics</topic><topic>Escherichia coli O157 - pathogenicity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetic Markers</topic><topic>Hemolysin proteins</topic><topic>Hemolysis</topic><topic>Hemolytic uremic syndrome</topic><topic>Humans</topic><topic>Major Articles</topic><topic>Microbiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Phenotype</topic><topic>Phenotypes</topic><topic>Plasmids</topic><topic>Plasmids - genetics</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>Serotyping</topic><topic>Shiga Toxins</topic><topic>Virulence</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schmidt, Herbert</creatorcontrib><creatorcontrib>Geitz, Caren</creatorcontrib><creatorcontrib>Tarr, Phillip I.</creatorcontrib><creatorcontrib>Frosch, Matthias</creatorcontrib><creatorcontrib>Karch, Helge</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schmidt, Herbert</au><au>Geitz, Caren</au><au>Tarr, Phillip I.</au><au>Frosch, Matthias</au><au>Karch, Helge</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Non-O157:H7 Pathogenic Shiga Toxin-Producing Escherichia coli: Phenotypic and Genetic Profiling of Virulence Traits and Evidence for Clonality</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>The Journal of Infectious Diseases</addtitle><date>1999-01</date><risdate>1999</risdate><volume>179</volume><issue>1</issue><spage>115</spage><epage>123</epage><pages>115-123</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>The virulence profiles of most non-O157 Shiga toxin (Stx)—producing Escherichia coli (STEC) are unknown. STEC belonging to serogroups O26,O103, and O111 were characterized to determine clonality and to profile virulence traits. Isolates within STEC serogroups O26, O111, and O103 were > 80% identical in their randomly amplified polymorphic DNA pattern, suggesting clonality in these serogroups. The serogroups were distantly related to each other and to E. coli O157:H7. stx restriction fragment length polymorphism differentiated some STEC with the same randomly amplified polymorphic DNA pattern. The hemolytic phenotype of STEC O103:H2 isolates resembled that of α- and not enterohemorrhagic E. coli hemolysin. Virulence gene (eae, E-hly, espP, etp, katP, stx) probing demonstrated serogroup-specific profiles. Pathogenic STEC O26, O103, and O111 belong to their own lineages and have unique profiles of virulence traits that are different from the virulence profile of E. coli O157:H7.</abstract><cop>Chicago, IL</cop><pub>University Chicago Press</pub><pmid>9841830</pmid><doi>10.1086/314537</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1899 |
| ispartof | The Journal of infectious diseases, 1999-01, Vol.179 (1), p.115-123 |
| issn | 0022-1899 1537-6613 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69547433 |
| source | MEDLINE; Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Bacterial Toxins - biosynthesis Bacterial Toxins - genetics Bacteriology Base Sequence Biological and medical sciences Diarrhea - microbiology DNA DNA Primers - genetics Escherichia coli Escherichia coli - classification Escherichia coli - genetics Escherichia coli - pathogenicity Escherichia coli Infections - microbiology Escherichia coli O157 Escherichia coli O157 - classification Escherichia coli O157 - genetics Escherichia coli O157 - pathogenicity Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic Markers Hemolysin proteins Hemolysis Hemolytic uremic syndrome Humans Major Articles Microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Phenotype Phenotypes Plasmids Plasmids - genetics Polymerase chain reaction Polymorphism, Restriction Fragment Length Random Amplified Polymorphic DNA Technique Serotyping Shiga Toxins Virulence Virulence - genetics |
| title | Non-O157:H7 Pathogenic Shiga Toxin-Producing Escherichia coli: Phenotypic and Genetic Profiling of Virulence Traits and Evidence for Clonality |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T03%3A11%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Non-O157:H7%20Pathogenic%20Shiga%20Toxin-Producing%20Escherichia%20coli:%20Phenotypic%20and%20Genetic%20Profiling%20of%20Virulence%20Traits%20and%20Evidence%20for%20Clonality&rft.jtitle=The%20Journal%20of%20infectious%20diseases&rft.au=Schmidt,%20Herbert&rft.date=1999-01&rft.volume=179&rft.issue=1&rft.spage=115&rft.epage=123&rft.pages=115-123&rft.issn=0022-1899&rft.eissn=1537-6613&rft.coden=JIDIAQ&rft_id=info:doi/10.1086/314537&rft_dat=%3Cjstor_proqu%3E30117234%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=223909262&rft_id=info:pmid/9841830&rft_jstor_id=30117234&rfr_iscdi=true |