Apoptosis Induced by Oxysterol in CEM Cells Is Associated with Negative Regulation of c-Myc
Previously we have demonstrated that treatment of the human lymphoblastic leukemic CEM cells with 25-hydroxycholesterol (25OHC) induces apoptosis. In the present study, we show that both c-mycmRNA and c-Myc protein levels are reduced only in oxysterol-sensitive and not in oxysterol-resistant cells a...
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| Veröffentlicht in: | Experimental cell research 1999-01, Vol.246 (1), p.193-202 |
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| creator | Ayala-Torres, S Zhou, F Thompson, E.B |
| description | Previously we have demonstrated that treatment of the human lymphoblastic leukemic CEM cells with 25-hydroxycholesterol (25OHC) induces apoptosis. In the present study, we show that both c-mycmRNA and c-Myc protein levels are reduced only in oxysterol-sensitive and not in oxysterol-resistant cells after treatment with concentrations of 25OHC that kill the sensitive CEM cells. The repression of c-Myc protein precedes c-mycmRNA reduction, and both events occur before the onset of cell death. Our data suggest that 25OHC-induced suppression of c-mycgene expression in CEM cells results from posttranscriptional regulation. These results demonstrate the regulation by an oxysterol of a gene/gene product important for cell growth and viability and an association between oxysterol-induced apoptosis of CEM cells and the negative regulation of c-myc. |
| doi_str_mv | 10.1006/excr.1998.4308 |
| format | Article |
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In the present study, we show that both c-mycmRNA and c-Myc protein levels are reduced only in oxysterol-sensitive and not in oxysterol-resistant cells after treatment with concentrations of 25OHC that kill the sensitive CEM cells. The repression of c-Myc protein precedes c-mycmRNA reduction, and both events occur before the onset of cell death. Our data suggest that 25OHC-induced suppression of c-mycgene expression in CEM cells results from posttranscriptional regulation. These results demonstrate the regulation by an oxysterol of a gene/gene product important for cell growth and viability and an association between oxysterol-induced apoptosis of CEM cells and the negative regulation of c-myc.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1006/excr.1998.4308</identifier><identifier>PMID: 9882528</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>apoptosis ; Apoptosis - drug effects ; Blotting, Northern ; Blotting, Western ; c-myc ; Caspases - metabolism ; Cell Division - drug effects ; Cell Nucleus - metabolism ; Cell Survival - drug effects ; CEM ; Down-Regulation - drug effects ; Genes, myc - genetics ; Half-Life ; Humans ; Hydroxycholesterols - pharmacology ; Leukemia, Lymphoid ; oxysterol ; posttranscriptional regulation ; Proto-Oncogene Proteins c-myc - genetics ; Proto-Oncogene Proteins c-myc - metabolism ; RNA Processing, Post-Transcriptional - drug effects ; RNA, Messenger - biosynthesis ; RNA, Messenger - metabolism ; Time Factors ; Transcription, Genetic - drug effects ; Tumor Cells, Cultured</subject><ispartof>Experimental cell research, 1999-01, Vol.246 (1), p.193-202</ispartof><rights>1999 Academic Press</rights><rights>Copyright 1999 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-be78ea7976f3cb5f175b1c50ce54683068db544536592bf9dea27eb7028c130b3</citedby><cites>FETCH-LOGICAL-c339t-be78ea7976f3cb5f175b1c50ce54683068db544536592bf9dea27eb7028c130b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/excr.1998.4308$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9882528$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ayala-Torres, S</creatorcontrib><creatorcontrib>Zhou, F</creatorcontrib><creatorcontrib>Thompson, E.B</creatorcontrib><title>Apoptosis Induced by Oxysterol in CEM Cells Is Associated with Negative Regulation of c-Myc</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Previously we have demonstrated that treatment of the human lymphoblastic leukemic CEM cells with 25-hydroxycholesterol (25OHC) induces apoptosis. In the present study, we show that both c-mycmRNA and c-Myc protein levels are reduced only in oxysterol-sensitive and not in oxysterol-resistant cells after treatment with concentrations of 25OHC that kill the sensitive CEM cells. The repression of c-Myc protein precedes c-mycmRNA reduction, and both events occur before the onset of cell death. Our data suggest that 25OHC-induced suppression of c-mycgene expression in CEM cells results from posttranscriptional regulation. These results demonstrate the regulation by an oxysterol of a gene/gene product important for cell growth and viability and an association between oxysterol-induced apoptosis of CEM cells and the negative regulation of c-myc.</description><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Blotting, Northern</subject><subject>Blotting, Western</subject><subject>c-myc</subject><subject>Caspases - metabolism</subject><subject>Cell Division - drug effects</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Survival - drug effects</subject><subject>CEM</subject><subject>Down-Regulation - drug effects</subject><subject>Genes, myc - genetics</subject><subject>Half-Life</subject><subject>Humans</subject><subject>Hydroxycholesterols - pharmacology</subject><subject>Leukemia, Lymphoid</subject><subject>oxysterol</subject><subject>posttranscriptional regulation</subject><subject>Proto-Oncogene Proteins c-myc - genetics</subject><subject>Proto-Oncogene Proteins c-myc - metabolism</subject><subject>RNA Processing, Post-Transcriptional - drug effects</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - metabolism</subject><subject>Time Factors</subject><subject>Transcription, Genetic - drug effects</subject><subject>Tumor Cells, Cultured</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kLtPwzAQhy0EgvJY2ZA8saX4ESf2WFXlIbVUQjAxWLFzAaM0LnYC9L_HVSs2pjvpvvvp7kPokpIxJaS4gR8bxlQpOc45kQdoRIkiGcsZO0QjQmie5ZKVJ-g0xg9CiJS0OEbHSkommByh18nar3sfXcQPXT1YqLHZ4OXPJvYQfItdh6ezBZ5C2yYi4kmM3rqqT9y369_xI7xVvfsC_ARvQ5ta32HfYJstNvYcHTVVG-FiX8_Qy-3seXqfzZd3D9PJPLOcqz4zUEqoSlUWDbdGNLQUhlpBLIi8kJwUsjYizwUvhGKmUTVUrARTEiYt5cTwM3S9y10H_zlA7PXKRZsurjrwQ9SFSuuKiwSOd6ANPsYAjV4Ht6rCRlOitzb11qbe2tRbm2nhap88mBXUf_heX5rL3RzSe18Ogo7WQZcsugC217V3_0X_Ag_zg0k</recordid><startdate>19990110</startdate><enddate>19990110</enddate><creator>Ayala-Torres, S</creator><creator>Zhou, F</creator><creator>Thompson, E.B</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990110</creationdate><title>Apoptosis Induced by Oxysterol in CEM Cells Is Associated with Negative Regulation of c-Myc</title><author>Ayala-Torres, S ; Zhou, F ; Thompson, E.B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-be78ea7976f3cb5f175b1c50ce54683068db544536592bf9dea27eb7028c130b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Blotting, Northern</topic><topic>Blotting, Western</topic><topic>c-myc</topic><topic>Caspases - metabolism</topic><topic>Cell Division - drug effects</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Survival - drug effects</topic><topic>CEM</topic><topic>Down-Regulation - drug effects</topic><topic>Genes, myc - genetics</topic><topic>Half-Life</topic><topic>Humans</topic><topic>Hydroxycholesterols - pharmacology</topic><topic>Leukemia, Lymphoid</topic><topic>oxysterol</topic><topic>posttranscriptional regulation</topic><topic>Proto-Oncogene Proteins c-myc - genetics</topic><topic>Proto-Oncogene Proteins c-myc - metabolism</topic><topic>RNA Processing, Post-Transcriptional - drug effects</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - metabolism</topic><topic>Time Factors</topic><topic>Transcription, Genetic - drug effects</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ayala-Torres, S</creatorcontrib><creatorcontrib>Zhou, F</creatorcontrib><creatorcontrib>Thompson, E.B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ayala-Torres, S</au><au>Zhou, F</au><au>Thompson, E.B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apoptosis Induced by Oxysterol in CEM Cells Is Associated with Negative Regulation of c-Myc</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1999-01-10</date><risdate>1999</risdate><volume>246</volume><issue>1</issue><spage>193</spage><epage>202</epage><pages>193-202</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>Previously we have demonstrated that treatment of the human lymphoblastic leukemic CEM cells with 25-hydroxycholesterol (25OHC) induces apoptosis. 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| ispartof | Experimental cell research, 1999-01, Vol.246 (1), p.193-202 |
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| language | eng |
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| subjects | apoptosis Apoptosis - drug effects Blotting, Northern Blotting, Western c-myc Caspases - metabolism Cell Division - drug effects Cell Nucleus - metabolism Cell Survival - drug effects CEM Down-Regulation - drug effects Genes, myc - genetics Half-Life Humans Hydroxycholesterols - pharmacology Leukemia, Lymphoid oxysterol posttranscriptional regulation Proto-Oncogene Proteins c-myc - genetics Proto-Oncogene Proteins c-myc - metabolism RNA Processing, Post-Transcriptional - drug effects RNA, Messenger - biosynthesis RNA, Messenger - metabolism Time Factors Transcription, Genetic - drug effects Tumor Cells, Cultured |
| title | Apoptosis Induced by Oxysterol in CEM Cells Is Associated with Negative Regulation of c-Myc |
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