An actin–myosin functional assay for analysis of smooth muscle (anti-microfilament) autoantibodies in human plasma
The detection of serum autoantibodies to smooth muscle (SMA) on rodent gastric mucosa by indirect immunofluorescence (IIF) has long been an immunodiagnostic marker for autoimmune hepatitis type 1 (AIH-1). The reactive antigenic moieties are cytoskeletal proteins which include polymeric F-actin as ju...
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| Veröffentlicht in: | Journal of immunological methods 2008-09, Vol.338 (1), p.63-66 |
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| creator | Martinez-Neira, R. dos Remedios, C.G. Mackay, I.R. |
| description | The detection of serum autoantibodies to smooth muscle (SMA) on rodent gastric mucosa by indirect immunofluorescence (IIF) has long been an immunodiagnostic marker for autoimmune hepatitis type 1 (AIH-1). The reactive antigenic moieties are cytoskeletal proteins which include polymeric F-actin as judged by the staining of microfilaments of tissue by IIF. However, their specificity for actin in AIH-1 can be and usually is uncertain. Using an
in vitro functional assay, we compared the effects of Fab fragments of immunoglobulin (IgG) prepared from SMA-positive plasma from two patients with the effects of Fabs from 10 healthy subjects. Fabs are incorporated into an assay where actin (the putative antigen) activates skeletal muscle heavy meromyosin (HMM) ATPase activity. The data from these functional assays provide new insights into the significance of anti-microfilament assays in the diagnosis, and perhaps also pathogenesis, of AIH-1. |
| doi_str_mv | 10.1016/j.jim.2008.07.011 |
| format | Article |
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in vitro functional assay, we compared the effects of Fab fragments of immunoglobulin (IgG) prepared from SMA-positive plasma from two patients with the effects of Fabs from 10 healthy subjects. Fabs are incorporated into an assay where actin (the putative antigen) activates skeletal muscle heavy meromyosin (HMM) ATPase activity. The data from these functional assays provide new insights into the significance of anti-microfilament assays in the diagnosis, and perhaps also pathogenesis, of AIH-1.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/j.jim.2008.07.011</identifier><identifier>PMID: 18691595</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Actin Cytoskeleton - immunology ; Actin-activated myosin ATPase activity ; Actins - physiology ; Adenosine Triphosphatases - metabolism ; Autoantibodies - blood ; Autoimmune hepatitis ; Biological and medical sciences ; Enzyme-Linked Immunosorbent Assay ; F-actin ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; G-actin ; Heavy meromyosin ; Humans ; Immunoglobulin Fab Fragments - immunology ; Molecular immunology ; Muscle, Smooth - immunology ; Myosin ; Myosin Subfragments - metabolism ; Smooth muscle antibodies ; Techniques</subject><ispartof>Journal of immunological methods, 2008-09, Vol.338 (1), p.63-66</ispartof><rights>2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-f3a8b1f30e303141521ea38405ea1785f82b4846a12823774ee04e886b247d393</citedby><cites>FETCH-LOGICAL-c478t-f3a8b1f30e303141521ea38405ea1785f82b4846a12823774ee04e886b247d393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jim.2008.07.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20685643$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18691595$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martinez-Neira, R.</creatorcontrib><creatorcontrib>dos Remedios, C.G.</creatorcontrib><creatorcontrib>Mackay, I.R.</creatorcontrib><title>An actin–myosin functional assay for analysis of smooth muscle (anti-microfilament) autoantibodies in human plasma</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>The detection of serum autoantibodies to smooth muscle (SMA) on rodent gastric mucosa by indirect immunofluorescence (IIF) has long been an immunodiagnostic marker for autoimmune hepatitis type 1 (AIH-1). The reactive antigenic moieties are cytoskeletal proteins which include polymeric F-actin as judged by the staining of microfilaments of tissue by IIF. However, their specificity for actin in AIH-1 can be and usually is uncertain. Using an
in vitro functional assay, we compared the effects of Fab fragments of immunoglobulin (IgG) prepared from SMA-positive plasma from two patients with the effects of Fabs from 10 healthy subjects. Fabs are incorporated into an assay where actin (the putative antigen) activates skeletal muscle heavy meromyosin (HMM) ATPase activity. The data from these functional assays provide new insights into the significance of anti-microfilament assays in the diagnosis, and perhaps also pathogenesis, of AIH-1.</description><subject>Actin Cytoskeleton - immunology</subject><subject>Actin-activated myosin ATPase activity</subject><subject>Actins - physiology</subject><subject>Adenosine Triphosphatases - metabolism</subject><subject>Autoantibodies - blood</subject><subject>Autoimmune hepatitis</subject><subject>Biological and medical sciences</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>F-actin</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>G-actin</subject><subject>Heavy meromyosin</subject><subject>Humans</subject><subject>Immunoglobulin Fab Fragments - immunology</subject><subject>Molecular immunology</subject><subject>Muscle, Smooth - immunology</subject><subject>Myosin</subject><subject>Myosin Subfragments - metabolism</subject><subject>Smooth muscle antibodies</subject><subject>Techniques</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQhyMEokvhAbggX0DlkDBjO7GjnqqKf1IlLnC2Jl5H9Sq2lziptDfegTfkSfBqV3CDkzWjb36256uqlwgNAnbvds3Oh4YD6AZUA4iPqg1qxWvVQ_u42gBwXqNq-4vqWc47AEDo4Gl1gbrrse3bTbXcREZ28fHXj5_hkLKPbFxjaaRIE6Oc6cDGNDMq5SH7zNLIckhpuWdhzXZy7Iri4uvg7ZxGP1FwcXnLaF3SsT-krXeZldT7NVBk-4lyoOfVk5Gm7F6cz8vq24f3X28_1XdfPn6-vbmrrVR6qUdBesBRgBMgUGLL0ZHQElpHqHQ7aj5ILTtCrrlQSjoH0mndDVyqrejFZfXmlLuf0_fV5cUEn62bJoourdl0fSs0aPwvyBG4ligKiCew_Dbn2Y1mP_tA88EgmKMTszPFiTk6MaBMcVJmXp3D1yG47d-Js4QCvD4DlC1N40zR-vyH49DptpPHy69PnCs7e_BuNtl6F63b-tnZxWyT_8czfgOw4qqv</recordid><startdate>20080930</startdate><enddate>20080930</enddate><creator>Martinez-Neira, R.</creator><creator>dos Remedios, C.G.</creator><creator>Mackay, I.R.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20080930</creationdate><title>An actin–myosin functional assay for analysis of smooth muscle (anti-microfilament) autoantibodies in human plasma</title><author>Martinez-Neira, R. ; dos Remedios, C.G. ; Mackay, I.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-f3a8b1f30e303141521ea38405ea1785f82b4846a12823774ee04e886b247d393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Actin Cytoskeleton - immunology</topic><topic>Actin-activated myosin ATPase activity</topic><topic>Actins - physiology</topic><topic>Adenosine Triphosphatases - metabolism</topic><topic>Autoantibodies - blood</topic><topic>Autoimmune hepatitis</topic><topic>Biological and medical sciences</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>F-actin</topic><topic>Fundamental and applied biological sciences. 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The reactive antigenic moieties are cytoskeletal proteins which include polymeric F-actin as judged by the staining of microfilaments of tissue by IIF. However, their specificity for actin in AIH-1 can be and usually is uncertain. Using an
in vitro functional assay, we compared the effects of Fab fragments of immunoglobulin (IgG) prepared from SMA-positive plasma from two patients with the effects of Fabs from 10 healthy subjects. Fabs are incorporated into an assay where actin (the putative antigen) activates skeletal muscle heavy meromyosin (HMM) ATPase activity. The data from these functional assays provide new insights into the significance of anti-microfilament assays in the diagnosis, and perhaps also pathogenesis, of AIH-1.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>18691595</pmid><doi>10.1016/j.jim.2008.07.011</doi><tpages>4</tpages></addata></record> |
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| subjects | Actin Cytoskeleton - immunology Actin-activated myosin ATPase activity Actins - physiology Adenosine Triphosphatases - metabolism Autoantibodies - blood Autoimmune hepatitis Biological and medical sciences Enzyme-Linked Immunosorbent Assay F-actin Fundamental and applied biological sciences. Psychology Fundamental immunology G-actin Heavy meromyosin Humans Immunoglobulin Fab Fragments - immunology Molecular immunology Muscle, Smooth - immunology Myosin Myosin Subfragments - metabolism Smooth muscle antibodies Techniques |
| title | An actin–myosin functional assay for analysis of smooth muscle (anti-microfilament) autoantibodies in human plasma |
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