[4] Comparison of enhanced green fluorescent protein and its destabilized form as transcription reporters

This chapter describes the utility of green fluorescent protein (GFP) as a reporter gene in the study of gene expression. GFP emits fluorescent light on excitation, without the addition of substrate or cofactor, both in vivo and in vitro. The GFP fluorescence activity can be detected using a fluores...

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Veröffentlicht in:	Methods in Enzymology 1999, Vol.302, p.32-38
Hauptverfasser:	Zhao, Xiaoning, Duong, Tommy, Huang, Chiao-Chian, Kain, Steven R., Li, Xianoiang
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line Dose-Response Relationship, Drug Genes, Reporter Green Fluorescent Proteins Humans Luminescent Proteins - metabolism Microscopy, Fluorescence - methods NF-kappa B - metabolism Protein Binding Signal Transduction Time Factors Transcription, Genetic Tumor Necrosis Factor-alpha - metabolism
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