Enhanced in vivo transgene expression and immunogenicity from plasmid vectors following electrostimulation in rodents and primates
Abstract Safe and efficient methods for in vivo delivery of transgenes of interest must be developed so that the promise of these therapies can be practically used in the clinic. In this work, we describe the use of electrostimulation to enhance the in vivo efficiency of plasmid DNA delivery. The me...
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| Veröffentlicht in: | Vaccine 2008-09, Vol.26 (40), p.5202-5209 |
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| container_title | Vaccine |
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| creator | Simon, Adam J Casimiro, Danilo R Finnefrock, Adam C Davies, Mary-Ellen Tang, Aimin Chen, Minchun Chastain, Michael Kath, Gary S Chen, Ling Shiver, John W |
| description | Abstract Safe and efficient methods for in vivo delivery of transgenes of interest must be developed so that the promise of these therapies can be practically used in the clinic. In this work, we describe the use of electrostimulation to enhance the in vivo efficiency of plasmid DNA delivery. The method was optimized to work over a range of moderate frequencies, utilizing low field strengths and simple symmetrical waveforms. After studying several parameters of delivery in mice, we demonstrate how this methodology can be employed to significantly improve both gene expression (over 16-fold) and the immunogenicity of HIV-1 vaccines (over 28-fold) compared to naked DNA in non-human primates. Compared to an efficient viral Ad5 vector system, the gene expression levels of DNA + electrostimulation were surprisingly within a factor of four of the viral delivery system. |
| doi_str_mv | 10.1016/j.vaccine.2008.03.058 |
| format | Article |
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In this work, we describe the use of electrostimulation to enhance the in vivo efficiency of plasmid DNA delivery. The method was optimized to work over a range of moderate frequencies, utilizing low field strengths and simple symmetrical waveforms. After studying several parameters of delivery in mice, we demonstrate how this methodology can be employed to significantly improve both gene expression (over 16-fold) and the immunogenicity of HIV-1 vaccines (over 28-fold) compared to naked DNA in non-human primates. Compared to an efficient viral Ad5 vector system, the gene expression levels of DNA + electrostimulation were surprisingly within a factor of four of the viral delivery system.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2008.03.058</identifier><identifier>PMID: 18472195</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>AIDS Vaccines - genetics ; AIDS Vaccines - immunology ; Alkaline Phosphatase - genetics ; Allergy and Immunology ; Animals ; Applied microbiology ; Biological and medical sciences ; Cell Line ; DNA vaccines ; Electroporation ; Electroporation - methods ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Transfer Techniques ; Genetic Vectors ; HIV Infections - immunology ; HIV Infections - prevention & control ; Human immunodeficiency virus 1 ; Humans ; Macaca ; Mice ; Mice, Inbred BALB C ; Microbiology ; Plasmids - genetics ; Primates ; Transgenes ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, DNA - genetics ; Vaccines, DNA - immunology</subject><ispartof>Vaccine, 2008-09, Vol.26 (40), p.5202-5209</ispartof><rights>Elsevier Ltd</rights><rights>2008 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-9c6b16a45000dc79d7901796000e0dbd0eb86d00b50a6eee295f68dab1d1dbb03</citedby><cites>FETCH-LOGICAL-c479t-9c6b16a45000dc79d7901796000e0dbd0eb86d00b50a6eee295f68dab1d1dbb03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X08003344$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20787633$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18472195$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simon, Adam J</creatorcontrib><creatorcontrib>Casimiro, Danilo R</creatorcontrib><creatorcontrib>Finnefrock, Adam C</creatorcontrib><creatorcontrib>Davies, Mary-Ellen</creatorcontrib><creatorcontrib>Tang, Aimin</creatorcontrib><creatorcontrib>Chen, Minchun</creatorcontrib><creatorcontrib>Chastain, Michael</creatorcontrib><creatorcontrib>Kath, Gary S</creatorcontrib><creatorcontrib>Chen, Ling</creatorcontrib><creatorcontrib>Shiver, John W</creatorcontrib><title>Enhanced in vivo transgene expression and immunogenicity from plasmid vectors following electrostimulation in rodents and primates</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract Safe and efficient methods for in vivo delivery of transgenes of interest must be developed so that the promise of these therapies can be practically used in the clinic. In this work, we describe the use of electrostimulation to enhance the in vivo efficiency of plasmid DNA delivery. The method was optimized to work over a range of moderate frequencies, utilizing low field strengths and simple symmetrical waveforms. After studying several parameters of delivery in mice, we demonstrate how this methodology can be employed to significantly improve both gene expression (over 16-fold) and the immunogenicity of HIV-1 vaccines (over 28-fold) compared to naked DNA in non-human primates. 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Psychology</subject><subject>Gene Expression</subject><subject>Gene Transfer Techniques</subject><subject>Genetic Vectors</subject><subject>HIV Infections - immunology</subject><subject>HIV Infections - prevention & control</subject><subject>Human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Macaca</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Plasmids - genetics</subject><subject>Primates</subject><subject>Transgenes</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, DNA - genetics</subject><subject>Vaccines, DNA - immunology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks-P1SAQgBujcZ-rf4KmF721DqU_4KIxm1012cSDmngjFKYrTwpPaKvvun-5dF-jiZc9EeCbGWY-suw5gZIAaV_vy0UqZRyWFQArgZbQsAfZjrCOFlVD2MNsB1VbFzWBb2fZkxj3ANBQwh9nZ4TVXUV4s8tuL9136RTq3Lh8MYvPpyBdvEGHOf4-BIzReJdLl4BxnJ1PN0aZ6ZgPwY_5wco4Gp0vqCYfYj54a_0v425ytOko-DiZcbZyWpOkCsFrdFO8y3cIZpQTxqfZo0HaiM-29Tz7enX55eJDcf3p_ceLd9eFqjs-FVy1PWll3aQ2tOq47jiQjrdpi6B7DdizVgP0DcgWESveDC3Tsiea6L4Hep69OuU9BP9zxjiJ0USF1kqHfo6i5Q2tGK_uBQnvCOM1SWBzAlVqNAYcxF1P4SgIiNWS2IvNklgtCaAiWUpxL7YCcz-i_he1aUnAyw2QUUk7JCXKxL9cBR3rWkoT9_bEYZrbYjCIqAyuOk1I4xfam3uf8ua_DMqaZFjaH3jEuPdzcEmKICJWAsTn9UutPwoYAKV1Tf8AIbDLxA</recordid><startdate>20080919</startdate><enddate>20080919</enddate><creator>Simon, Adam J</creator><creator>Casimiro, Danilo R</creator><creator>Finnefrock, Adam C</creator><creator>Davies, Mary-Ellen</creator><creator>Tang, Aimin</creator><creator>Chen, Minchun</creator><creator>Chastain, Michael</creator><creator>Kath, Gary S</creator><creator>Chen, Ling</creator><creator>Shiver, John W</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080919</creationdate><title>Enhanced in vivo transgene expression and immunogenicity from plasmid vectors following electrostimulation in rodents and primates</title><author>Simon, Adam J ; Casimiro, Danilo R ; Finnefrock, Adam C ; Davies, Mary-Ellen ; Tang, Aimin ; Chen, Minchun ; Chastain, Michael ; Kath, Gary S ; Chen, Ling ; Shiver, John W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-9c6b16a45000dc79d7901796000e0dbd0eb86d00b50a6eee295f68dab1d1dbb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>AIDS Vaccines - genetics</topic><topic>AIDS Vaccines - immunology</topic><topic>Alkaline Phosphatase - genetics</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>DNA vaccines</topic><topic>Electroporation</topic><topic>Electroporation - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Transfer Techniques</topic><topic>Genetic Vectors</topic><topic>HIV Infections - immunology</topic><topic>HIV Infections - prevention & control</topic><topic>Human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Macaca</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Plasmids - genetics</topic><topic>Primates</topic><topic>Transgenes</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccines, DNA - genetics</topic><topic>Vaccines, DNA - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simon, Adam J</creatorcontrib><creatorcontrib>Casimiro, Danilo R</creatorcontrib><creatorcontrib>Finnefrock, Adam C</creatorcontrib><creatorcontrib>Davies, Mary-Ellen</creatorcontrib><creatorcontrib>Tang, Aimin</creatorcontrib><creatorcontrib>Chen, Minchun</creatorcontrib><creatorcontrib>Chastain, Michael</creatorcontrib><creatorcontrib>Kath, Gary S</creatorcontrib><creatorcontrib>Chen, Ling</creatorcontrib><creatorcontrib>Shiver, John W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simon, Adam J</au><au>Casimiro, Danilo R</au><au>Finnefrock, Adam C</au><au>Davies, Mary-Ellen</au><au>Tang, Aimin</au><au>Chen, Minchun</au><au>Chastain, Michael</au><au>Kath, Gary S</au><au>Chen, Ling</au><au>Shiver, John W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced in vivo transgene expression and immunogenicity from plasmid vectors following electrostimulation in rodents and primates</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2008-09-19</date><risdate>2008</risdate><volume>26</volume><issue>40</issue><spage>5202</spage><epage>5209</epage><pages>5202-5209</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract Safe and efficient methods for in vivo delivery of transgenes of interest must be developed so that the promise of these therapies can be practically used in the clinic. 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| ispartof | Vaccine, 2008-09, Vol.26 (40), p.5202-5209 |
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| subjects | AIDS Vaccines - genetics AIDS Vaccines - immunology Alkaline Phosphatase - genetics Allergy and Immunology Animals Applied microbiology Biological and medical sciences Cell Line DNA vaccines Electroporation Electroporation - methods Fundamental and applied biological sciences. Psychology Gene Expression Gene Transfer Techniques Genetic Vectors HIV Infections - immunology HIV Infections - prevention & control Human immunodeficiency virus 1 Humans Macaca Mice Mice, Inbred BALB C Microbiology Plasmids - genetics Primates Transgenes Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, DNA - genetics Vaccines, DNA - immunology |
| title | Enhanced in vivo transgene expression and immunogenicity from plasmid vectors following electrostimulation in rodents and primates |
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