In vitro and in vivo bioactivity of recombinant canine hepatocyte growth factor
Hepatocyte growth factor (HGF) is crucial for the development and regeneration of the liver and offers a possible new therapeutic strategy for the treatment of canine liver disease. In this study, the in vitro and in vivo bioactivity of recombinant canine HGF (rcHGF) produced with a baculoviral expr...
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| Veröffentlicht in: | The veterinary journal (1997) 2008-10, Vol.178 (1), p.70-77 |
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| creator | Arends, Brigitte Spee, Bart Hoffmann, Gaby Jansen, Georgina E.G. Slump, Estel Auriemma, Edoardo IJzer, Jooske Hemrika, Wieger Romijn, Roland A. van der Heijden-Liefkens, Karin H.A. Sondermeijer, Paul J.A. van den Ingh, Ted S.G.A.M. Penning, Louis C. Rothuizen, Jan |
| description | Hepatocyte growth factor (HGF) is crucial for the development and regeneration of the liver and offers a possible new therapeutic strategy for the treatment of canine liver disease. In this study, the in vitro and in vivo bioactivity of recombinant canine HGF (rcHGF) produced with a baculoviral expression system in insect cells was measured.
In vitro rcHGF induced mitogenesis, motogenesis, and phosphorylated the HGF receptor c-MET and its downstream mediators PKB and ERK1/2 in two canine epithelial cell lines. After a partial hepatectomy (phx) in dogs, rcHGF increased phosphorylation of c-MET, PKB and ERK1/2. A moderate increase was seen with the cell cycle protein PCNA in rcHGF treated livers, but no HGF-induced increase in liver weight was seen 7 days after phx. Furthermore, rcHGF treated livers showed lower levels of the key mediator of apoptosis, caspase-3, at 7
days after phx. It is concluded that rcHGF is a biologically active protein in vitro and in vivo and the baculoviral expression system supplies sufficient amounts of rcHGF for future clinical studies in dogs. |
| doi_str_mv | 10.1016/j.tvjl.2007.11.002 |
| format | Article |
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In vitro rcHGF induced mitogenesis, motogenesis, and phosphorylated the HGF receptor c-MET and its downstream mediators PKB and ERK1/2 in two canine epithelial cell lines. After a partial hepatectomy (phx) in dogs, rcHGF increased phosphorylation of c-MET, PKB and ERK1/2. A moderate increase was seen with the cell cycle protein PCNA in rcHGF treated livers, but no HGF-induced increase in liver weight was seen 7 days after phx. Furthermore, rcHGF treated livers showed lower levels of the key mediator of apoptosis, caspase-3, at 7
days after phx. It is concluded that rcHGF is a biologically active protein in vitro and in vivo and the baculoviral expression system supplies sufficient amounts of rcHGF for future clinical studies in dogs.</description><identifier>ISSN: 1090-0233</identifier><identifier>EISSN: 1532-2971</identifier><identifier>DOI: 10.1016/j.tvjl.2007.11.002</identifier><identifier>PMID: 18314358</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; apoptosis ; bioactive properties ; biochemical pathways ; c-MET ; Cell Line ; dog diseases ; Dogs ; epithelial cells ; ERK1/2 ; excision ; Extracellular Signal-Regulated MAP Kinases - genetics ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Female ; Gene Expression Regulation - drug effects ; Hepatectomy ; hepatocyte growth factor ; Hepatocyte Growth Factor - genetics ; Hepatocyte Growth Factor - metabolism ; Hepatocytes - drug effects ; HGF ; in vitro studies ; in vivo studies ; liver ; Liver - drug effects ; Liver - physiology ; liver diseases ; Liver regeneration ; Liver Regeneration - drug effects ; mitosis ; oncogenes ; Organ Size ; Partial hepatectomy ; PCNA ; phosphorylation ; physiological response ; PKB ; Proto-Oncogene Proteins c-akt - genetics ; Proto-Oncogene Proteins c-akt - metabolism ; Proto-Oncogene Proteins c-met - genetics ; Proto-Oncogene Proteins c-met - metabolism ; Recombinant Proteins</subject><ispartof>The veterinary journal (1997), 2008-10, Vol.178 (1), p.70-77</ispartof><rights>2007 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-6faad4150b7cf9ae0be4335fb23b8761e63b7ae00cdf5aa6bc3955d3f02a560a3</citedby><cites>FETCH-LOGICAL-c422t-6faad4150b7cf9ae0be4335fb23b8761e63b7ae00cdf5aa6bc3955d3f02a560a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tvjl.2007.11.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18314358$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arends, Brigitte</creatorcontrib><creatorcontrib>Spee, Bart</creatorcontrib><creatorcontrib>Hoffmann, Gaby</creatorcontrib><creatorcontrib>Jansen, Georgina E.G.</creatorcontrib><creatorcontrib>Slump, Estel</creatorcontrib><creatorcontrib>Auriemma, Edoardo</creatorcontrib><creatorcontrib>IJzer, Jooske</creatorcontrib><creatorcontrib>Hemrika, Wieger</creatorcontrib><creatorcontrib>Romijn, Roland A.</creatorcontrib><creatorcontrib>van der Heijden-Liefkens, Karin H.A.</creatorcontrib><creatorcontrib>Sondermeijer, Paul J.A.</creatorcontrib><creatorcontrib>van den Ingh, Ted S.G.A.M.</creatorcontrib><creatorcontrib>Penning, Louis C.</creatorcontrib><creatorcontrib>Rothuizen, Jan</creatorcontrib><title>In vitro and in vivo bioactivity of recombinant canine hepatocyte growth factor</title><title>The veterinary journal (1997)</title><addtitle>Vet J</addtitle><description>Hepatocyte growth factor (HGF) is crucial for the development and regeneration of the liver and offers a possible new therapeutic strategy for the treatment of canine liver disease. In this study, the in vitro and in vivo bioactivity of recombinant canine HGF (rcHGF) produced with a baculoviral expression system in insect cells was measured.
In vitro rcHGF induced mitogenesis, motogenesis, and phosphorylated the HGF receptor c-MET and its downstream mediators PKB and ERK1/2 in two canine epithelial cell lines. After a partial hepatectomy (phx) in dogs, rcHGF increased phosphorylation of c-MET, PKB and ERK1/2. A moderate increase was seen with the cell cycle protein PCNA in rcHGF treated livers, but no HGF-induced increase in liver weight was seen 7 days after phx. Furthermore, rcHGF treated livers showed lower levels of the key mediator of apoptosis, caspase-3, at 7
days after phx. It is concluded that rcHGF is a biologically active protein in vitro and in vivo and the baculoviral expression system supplies sufficient amounts of rcHGF for future clinical studies in dogs.</description><subject>Animals</subject><subject>apoptosis</subject><subject>bioactive properties</subject><subject>biochemical pathways</subject><subject>c-MET</subject><subject>Cell Line</subject><subject>dog diseases</subject><subject>Dogs</subject><subject>epithelial cells</subject><subject>ERK1/2</subject><subject>excision</subject><subject>Extracellular Signal-Regulated MAP Kinases - genetics</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Hepatectomy</subject><subject>hepatocyte growth factor</subject><subject>Hepatocyte Growth Factor - genetics</subject><subject>Hepatocyte Growth Factor - metabolism</subject><subject>Hepatocytes - drug effects</subject><subject>HGF</subject><subject>in vitro studies</subject><subject>in vivo studies</subject><subject>liver</subject><subject>Liver - drug effects</subject><subject>Liver - physiology</subject><subject>liver diseases</subject><subject>Liver regeneration</subject><subject>Liver Regeneration - drug effects</subject><subject>mitosis</subject><subject>oncogenes</subject><subject>Organ Size</subject><subject>Partial hepatectomy</subject><subject>PCNA</subject><subject>phosphorylation</subject><subject>physiological response</subject><subject>PKB</subject><subject>Proto-Oncogene Proteins c-akt - genetics</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Proto-Oncogene Proteins c-met - genetics</subject><subject>Proto-Oncogene Proteins c-met - metabolism</subject><subject>Recombinant Proteins</subject><issn>1090-0233</issn><issn>1532-2971</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1vGyEQhlHVqvlo_0APLafedjPAsuuVeqmi5kOKlEObMxrYIcGyFxewI__7YtlSbzkBM8_7Cj2MfRHQChD91bItu-WqlQBDK0QLIN-xc6GVbOQ4iPf1DiM0IJU6Yxc5LwFg7Dr5kZ2JhRKd0otz9ng_810oKXKcJx4Oj13kNkR0JdTFnkfPE7m4tmHGuXCHc5iJv9AGS3T7Qvw5xdfywn1NxPSJffC4yvT5dF6yp5tff67vmofH2_vrnw-N66QsTe8Rp05osIPzIxJY6pTS3kplF0MvqFd2qGNwk9eIvXVq1HpSHiTqHlBdsu_H3k2Kf7eUi1mH7Gi1wpniNpt-1LIb1aKC8gi6FHNO5M0mhTWmvRFgDhrN0hw0moNGI4SpGmvo66l9a9c0_Y-cvFXg2xHwGA0-p5DN028JQkH1PyoFlfhxJKha2AVKJrtAs6MpVJ3FTDG89YN_7G-OKA</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>Arends, Brigitte</creator><creator>Spee, Bart</creator><creator>Hoffmann, Gaby</creator><creator>Jansen, Georgina E.G.</creator><creator>Slump, Estel</creator><creator>Auriemma, Edoardo</creator><creator>IJzer, Jooske</creator><creator>Hemrika, Wieger</creator><creator>Romijn, Roland A.</creator><creator>van der Heijden-Liefkens, Karin H.A.</creator><creator>Sondermeijer, Paul J.A.</creator><creator>van den Ingh, Ted S.G.A.M.</creator><creator>Penning, Louis C.</creator><creator>Rothuizen, Jan</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20081001</creationdate><title>In vitro and in vivo bioactivity of recombinant canine hepatocyte growth factor</title><author>Arends, Brigitte ; Spee, Bart ; Hoffmann, Gaby ; Jansen, Georgina E.G. ; Slump, Estel ; Auriemma, Edoardo ; IJzer, Jooske ; Hemrika, Wieger ; Romijn, Roland A. ; van der Heijden-Liefkens, Karin H.A. ; Sondermeijer, Paul J.A. ; van den Ingh, Ted S.G.A.M. ; Penning, Louis C. ; Rothuizen, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-6faad4150b7cf9ae0be4335fb23b8761e63b7ae00cdf5aa6bc3955d3f02a560a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>apoptosis</topic><topic>bioactive properties</topic><topic>biochemical pathways</topic><topic>c-MET</topic><topic>Cell Line</topic><topic>dog diseases</topic><topic>Dogs</topic><topic>epithelial cells</topic><topic>ERK1/2</topic><topic>excision</topic><topic>Extracellular Signal-Regulated MAP Kinases - genetics</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Hepatectomy</topic><topic>hepatocyte growth factor</topic><topic>Hepatocyte Growth Factor - genetics</topic><topic>Hepatocyte Growth Factor - metabolism</topic><topic>Hepatocytes - drug effects</topic><topic>HGF</topic><topic>in vitro studies</topic><topic>in vivo studies</topic><topic>liver</topic><topic>Liver - drug effects</topic><topic>Liver - physiology</topic><topic>liver diseases</topic><topic>Liver regeneration</topic><topic>Liver Regeneration - drug effects</topic><topic>mitosis</topic><topic>oncogenes</topic><topic>Organ Size</topic><topic>Partial hepatectomy</topic><topic>PCNA</topic><topic>phosphorylation</topic><topic>physiological response</topic><topic>PKB</topic><topic>Proto-Oncogene Proteins c-akt - genetics</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>Proto-Oncogene Proteins c-met - genetics</topic><topic>Proto-Oncogene Proteins c-met - metabolism</topic><topic>Recombinant Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arends, Brigitte</creatorcontrib><creatorcontrib>Spee, Bart</creatorcontrib><creatorcontrib>Hoffmann, Gaby</creatorcontrib><creatorcontrib>Jansen, Georgina E.G.</creatorcontrib><creatorcontrib>Slump, Estel</creatorcontrib><creatorcontrib>Auriemma, Edoardo</creatorcontrib><creatorcontrib>IJzer, Jooske</creatorcontrib><creatorcontrib>Hemrika, Wieger</creatorcontrib><creatorcontrib>Romijn, Roland A.</creatorcontrib><creatorcontrib>van der Heijden-Liefkens, Karin H.A.</creatorcontrib><creatorcontrib>Sondermeijer, Paul J.A.</creatorcontrib><creatorcontrib>van den Ingh, Ted S.G.A.M.</creatorcontrib><creatorcontrib>Penning, Louis C.</creatorcontrib><creatorcontrib>Rothuizen, Jan</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The veterinary journal (1997)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arends, Brigitte</au><au>Spee, Bart</au><au>Hoffmann, Gaby</au><au>Jansen, Georgina E.G.</au><au>Slump, Estel</au><au>Auriemma, Edoardo</au><au>IJzer, Jooske</au><au>Hemrika, Wieger</au><au>Romijn, Roland A.</au><au>van der Heijden-Liefkens, Karin H.A.</au><au>Sondermeijer, Paul J.A.</au><au>van den Ingh, Ted S.G.A.M.</au><au>Penning, Louis C.</au><au>Rothuizen, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro and in vivo bioactivity of recombinant canine hepatocyte growth factor</atitle><jtitle>The veterinary journal (1997)</jtitle><addtitle>Vet J</addtitle><date>2008-10-01</date><risdate>2008</risdate><volume>178</volume><issue>1</issue><spage>70</spage><epage>77</epage><pages>70-77</pages><issn>1090-0233</issn><eissn>1532-2971</eissn><abstract>Hepatocyte growth factor (HGF) is crucial for the development and regeneration of the liver and offers a possible new therapeutic strategy for the treatment of canine liver disease. In this study, the in vitro and in vivo bioactivity of recombinant canine HGF (rcHGF) produced with a baculoviral expression system in insect cells was measured.
In vitro rcHGF induced mitogenesis, motogenesis, and phosphorylated the HGF receptor c-MET and its downstream mediators PKB and ERK1/2 in two canine epithelial cell lines. After a partial hepatectomy (phx) in dogs, rcHGF increased phosphorylation of c-MET, PKB and ERK1/2. A moderate increase was seen with the cell cycle protein PCNA in rcHGF treated livers, but no HGF-induced increase in liver weight was seen 7 days after phx. Furthermore, rcHGF treated livers showed lower levels of the key mediator of apoptosis, caspase-3, at 7
days after phx. It is concluded that rcHGF is a biologically active protein in vitro and in vivo and the baculoviral expression system supplies sufficient amounts of rcHGF for future clinical studies in dogs.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>18314358</pmid><doi>10.1016/j.tvjl.2007.11.002</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals apoptosis bioactive properties biochemical pathways c-MET Cell Line dog diseases Dogs epithelial cells ERK1/2 excision Extracellular Signal-Regulated MAP Kinases - genetics Extracellular Signal-Regulated MAP Kinases - metabolism Female Gene Expression Regulation - drug effects Hepatectomy hepatocyte growth factor Hepatocyte Growth Factor - genetics Hepatocyte Growth Factor - metabolism Hepatocytes - drug effects HGF in vitro studies in vivo studies liver Liver - drug effects Liver - physiology liver diseases Liver regeneration Liver Regeneration - drug effects mitosis oncogenes Organ Size Partial hepatectomy PCNA phosphorylation physiological response PKB Proto-Oncogene Proteins c-akt - genetics Proto-Oncogene Proteins c-akt - metabolism Proto-Oncogene Proteins c-met - genetics Proto-Oncogene Proteins c-met - metabolism Recombinant Proteins |
| title | In vitro and in vivo bioactivity of recombinant canine hepatocyte growth factor |
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