Effect of integrin on procollagen synthesis by fibroblasts from scleroderma
To investigate the effect of integrin on fibroblasts from scleroderma in the production of procollagen. Phosphorothioate modified antisense oligonucleotides were used to interfere with the expression of integrin alpha 5 or beta 1 subunit on fibroblasts from 10 cases of scleroderma, and then the chan...
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| Veröffentlicht in: | Chinese medical journal 1999-11, Vol.112 (11), p.1024-1027 |
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| creator | Hong, W Chen, M Kong, X Liao, W |
| description | To investigate the effect of integrin on fibroblasts from scleroderma in the production of procollagen.
Phosphorothioate modified antisense oligonucleotides were used to interfere with the expression of integrin alpha 5 or beta 1 subunit on fibroblasts from 10 cases of scleroderma, and then the changes of procollagen mRNA due to the decline in level of integrin were observed by using RT-PCR.
Expression of integrin alpha 5 or beta 1 subunit was specifically inhibited by their corresponding antisense oligonucleotides. Fibroblasts with decreased expression of alpha 5 or beta 1 subunit produced lower level of procollagen alpha 1 (I), alpha 1 (III) mRNA in comparison to the control.
Overproduction of procollagen may be inhibited at the level of transcription by lowering the expression of integrin alpha 5 or beta 1 subunit on fibroblasts in scleroderma. |
| format | Article |
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Phosphorothioate modified antisense oligonucleotides were used to interfere with the expression of integrin alpha 5 or beta 1 subunit on fibroblasts from 10 cases of scleroderma, and then the changes of procollagen mRNA due to the decline in level of integrin were observed by using RT-PCR.
Expression of integrin alpha 5 or beta 1 subunit was specifically inhibited by their corresponding antisense oligonucleotides. Fibroblasts with decreased expression of alpha 5 or beta 1 subunit produced lower level of procollagen alpha 1 (I), alpha 1 (III) mRNA in comparison to the control.
Overproduction of procollagen may be inhibited at the level of transcription by lowering the expression of integrin alpha 5 or beta 1 subunit on fibroblasts in scleroderma.</description><identifier>ISSN: 0366-6999</identifier><identifier>PMID: 11721465</identifier><language>eng</language><publisher>China</publisher><subject>Adult ; Antigens, CD - biosynthesis ; Cells, Cultured ; Female ; Fibroblasts - metabolism ; Fibroblasts - pathology ; Humans ; Integrin alpha5 ; Integrin beta1 - biosynthesis ; Male ; Procollagen - biosynthesis ; Procollagen - genetics ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Scleroderma, Systemic - metabolism ; Scleroderma, Systemic - pathology ; Skin - metabolism ; Skin - pathology</subject><ispartof>Chinese medical journal, 1999-11, Vol.112 (11), p.1024-1027</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11721465$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hong, W</creatorcontrib><creatorcontrib>Chen, M</creatorcontrib><creatorcontrib>Kong, X</creatorcontrib><creatorcontrib>Liao, W</creatorcontrib><title>Effect of integrin on procollagen synthesis by fibroblasts from scleroderma</title><title>Chinese medical journal</title><addtitle>Chin Med J (Engl)</addtitle><description>To investigate the effect of integrin on fibroblasts from scleroderma in the production of procollagen.
Phosphorothioate modified antisense oligonucleotides were used to interfere with the expression of integrin alpha 5 or beta 1 subunit on fibroblasts from 10 cases of scleroderma, and then the changes of procollagen mRNA due to the decline in level of integrin were observed by using RT-PCR.
Expression of integrin alpha 5 or beta 1 subunit was specifically inhibited by their corresponding antisense oligonucleotides. Fibroblasts with decreased expression of alpha 5 or beta 1 subunit produced lower level of procollagen alpha 1 (I), alpha 1 (III) mRNA in comparison to the control.
Overproduction of procollagen may be inhibited at the level of transcription by lowering the expression of integrin alpha 5 or beta 1 subunit on fibroblasts in scleroderma.</description><subject>Adult</subject><subject>Antigens, CD - biosynthesis</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - pathology</subject><subject>Humans</subject><subject>Integrin alpha5</subject><subject>Integrin beta1 - biosynthesis</subject><subject>Male</subject><subject>Procollagen - biosynthesis</subject><subject>Procollagen - genetics</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Scleroderma, Systemic - metabolism</subject><subject>Scleroderma, Systemic - pathology</subject><subject>Skin - metabolism</subject><subject>Skin - pathology</subject><issn>0366-6999</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1j7tOwzAYRj2AaCm8AvLEFsl3JyOqykWtxAJzZDu_i1ESB9sZ8vZEokzfcvTpnCu0JVypSjVNs0G3OX8TwqTU6gZtKNWMCiW36HjwHlzB0eMwFjinMOI44ilFF_venGHEeRnLF-SQsV2wDzZF25tcMvYpDji7HlLsIA3mDl1702e4v-wOfT4fPvav1en95W3_dKomRnSplANpqSEgVOeooB3jTmrram4581rKptayFrYBoWnnRSeJ5UZbwzirtSJ8hx7_flfLnxlyaYeQHay6I8Q5t6qRjAilVvDhAs52gK6dUhhMWtr_fP4LQ0tV5w</recordid><startdate>199911</startdate><enddate>199911</enddate><creator>Hong, W</creator><creator>Chen, M</creator><creator>Kong, X</creator><creator>Liao, W</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199911</creationdate><title>Effect of integrin on procollagen synthesis by fibroblasts from scleroderma</title><author>Hong, W ; Chen, M ; Kong, X ; Liao, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p207t-6ce5b1a0e46dc141d23c57bc83b32f755987584b9e471df4d50b3a7ba23287603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>Antigens, CD - biosynthesis</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - pathology</topic><topic>Humans</topic><topic>Integrin alpha5</topic><topic>Integrin beta1 - biosynthesis</topic><topic>Male</topic><topic>Procollagen - biosynthesis</topic><topic>Procollagen - genetics</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Scleroderma, Systemic - metabolism</topic><topic>Scleroderma, Systemic - pathology</topic><topic>Skin - metabolism</topic><topic>Skin - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hong, W</creatorcontrib><creatorcontrib>Chen, M</creatorcontrib><creatorcontrib>Kong, X</creatorcontrib><creatorcontrib>Liao, W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hong, W</au><au>Chen, M</au><au>Kong, X</au><au>Liao, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of integrin on procollagen synthesis by fibroblasts from scleroderma</atitle><jtitle>Chinese medical journal</jtitle><addtitle>Chin Med J (Engl)</addtitle><date>1999-11</date><risdate>1999</risdate><volume>112</volume><issue>11</issue><spage>1024</spage><epage>1027</epage><pages>1024-1027</pages><issn>0366-6999</issn><abstract>To investigate the effect of integrin on fibroblasts from scleroderma in the production of procollagen.
Phosphorothioate modified antisense oligonucleotides were used to interfere with the expression of integrin alpha 5 or beta 1 subunit on fibroblasts from 10 cases of scleroderma, and then the changes of procollagen mRNA due to the decline in level of integrin were observed by using RT-PCR.
Expression of integrin alpha 5 or beta 1 subunit was specifically inhibited by their corresponding antisense oligonucleotides. Fibroblasts with decreased expression of alpha 5 or beta 1 subunit produced lower level of procollagen alpha 1 (I), alpha 1 (III) mRNA in comparison to the control.
Overproduction of procollagen may be inhibited at the level of transcription by lowering the expression of integrin alpha 5 or beta 1 subunit on fibroblasts in scleroderma.</abstract><cop>China</cop><pmid>11721465</pmid><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0366-6999 |
| ispartof | Chinese medical journal, 1999-11, Vol.112 (11), p.1024-1027 |
| issn | 0366-6999 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69520466 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adult Antigens, CD - biosynthesis Cells, Cultured Female Fibroblasts - metabolism Fibroblasts - pathology Humans Integrin alpha5 Integrin beta1 - biosynthesis Male Procollagen - biosynthesis Procollagen - genetics RNA, Messenger - biosynthesis RNA, Messenger - genetics Scleroderma, Systemic - metabolism Scleroderma, Systemic - pathology Skin - metabolism Skin - pathology |
| title | Effect of integrin on procollagen synthesis by fibroblasts from scleroderma |
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