Increased senescence and reduced functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction
Objective The aim of this study was to investigate the number and functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction. Study Design Fetal endothelial progenitor cells were isolated, and counted from 17 women with...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 2008-09, Vol.199 (3), p.259.e1-259.e7 |
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| container_title | American journal of obstetrics and gynecology |
| container_volume | 199 |
| creator | Hwang, Han-Sung, MD Maeng, Yong-Sun, MS Park, Yong-Won, MD, PhD Koos, Brian J., MD, PhD Kwon, Young-Guen, PhD Kim, Young-Han, MD, PhD |
| description | Objective The aim of this study was to investigate the number and functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction. Study Design Fetal endothelial progenitor cells were isolated, and counted from 17 women with preeclampsia without intrauterine growth restricion and 30 normal women. Colony-forming assay and differentiation time assay were performed to detect functional activity of the cells. To assess cellular senescence, senescence-associated β-galactosidase staining was performed for endothelial progenitor cells. Results Compared with normal pregnancy, the number of endothelial progenitor cells was significantly lower, differentiation time from endothelial progenitor cell into outgrowing cell was longer, and the number of colonies after differentiation was smaller in preeclampsia ( P < .001), respectively. The intensity of senescence-associated β-galactosidase staining was higher in preeclamptic pregnancy ( P < .001). Conclusion The number and functional ability of fetal endothelial progenitor cells from preeclampsia without intrauterine growth restriction are significantly decreased and they are more senescent compared with those of normal pregnancy. |
| doi_str_mv | 10.1016/j.ajog.2008.06.060 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69515366</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0002937808006923</els_id><sourcerecordid>69515366</sourcerecordid><originalsourceid>FETCH-LOGICAL-c439t-b1f1acb913574bdf943d01db822dadf9a7bf12907326f4aa82baaec7230c2b7d3</originalsourceid><addsrcrecordid>eNp9Uk2LFDEQbURx19U_4EFy0duM-ZhJOiDCsvixsOBBPYfqpHomYyYZk7TL_Cd_pGlnUPAgFCRVea9SVa-67jmjS0aZfL1bwi5tlpzSfkllM_qgu2RUq4XsZf-wu6SU8oUWqr_onpSym12u-ePugvVKMa3Wl93P22gzQkFHCkYsFqNFAtGRjG6yLTxO0VafIgQCgw--HkkayYi1BTC6VLcYfLsfctpg9DVlYjGEQnxsMdxEiNZjITbtD8FbqC3ncJyf0AbYH4oHcu_rNk21UWqGqWL2Eckmp_u6bXWUmv3vEp52j0YIBZ-dz6vu6_t3X24-Lu4-fbi9ub5b2JXQdTGwkYEdNBNrtRrcqFfCUeaGnnMHzQU1jIxrqgSX4wqg5wMAWsUFtXxQTlx1r055W0_fp_a_2fsyNwUR01SM1Gu2FlI2ID8BbU6lZBzNIfs95KNh1MwamZ2ZNTKzRobKZrSRXpyzT8Me3V_KWZQGeHkGQLEQxjxPsPzBcSqFWnPdcG9OOGyz-OExm9Im3fRzPqOtxiX__zre_kO3wcemUPiGRyy7NOUmejHMFG6o-Tyvz7xMtKdUai7ELwrIyys</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69515366</pqid></control><display><type>article</type><title>Increased senescence and reduced functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Hwang, Han-Sung, MD ; Maeng, Yong-Sun, MS ; Park, Yong-Won, MD, PhD ; Koos, Brian J., MD, PhD ; Kwon, Young-Guen, PhD ; Kim, Young-Han, MD, PhD</creator><creatorcontrib>Hwang, Han-Sung, MD ; Maeng, Yong-Sun, MS ; Park, Yong-Won, MD, PhD ; Koos, Brian J., MD, PhD ; Kwon, Young-Guen, PhD ; Kim, Young-Han, MD, PhD</creatorcontrib><description>Objective The aim of this study was to investigate the number and functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction. Study Design Fetal endothelial progenitor cells were isolated, and counted from 17 women with preeclampsia without intrauterine growth restricion and 30 normal women. Colony-forming assay and differentiation time assay were performed to detect functional activity of the cells. To assess cellular senescence, senescence-associated β-galactosidase staining was performed for endothelial progenitor cells. Results Compared with normal pregnancy, the number of endothelial progenitor cells was significantly lower, differentiation time from endothelial progenitor cell into outgrowing cell was longer, and the number of colonies after differentiation was smaller in preeclampsia ( P < .001), respectively. The intensity of senescence-associated β-galactosidase staining was higher in preeclamptic pregnancy ( P < .001). Conclusion The number and functional ability of fetal endothelial progenitor cells from preeclampsia without intrauterine growth restriction are significantly decreased and they are more senescent compared with those of normal pregnancy.</description><identifier>ISSN: 0002-9378</identifier><identifier>EISSN: 1097-6868</identifier><identifier>DOI: 10.1016/j.ajog.2008.06.060</identifier><identifier>PMID: 18771975</identifier><identifier>CODEN: AJOGAH</identifier><language>eng</language><publisher>Philadelphia, PA: Mosby, Inc</publisher><subject>Adult ; beta-Galactosidase - analysis ; Biological and medical sciences ; Cell Differentiation ; Cells, Cultured ; Cellular Senescence ; Colony-Forming Units Assay ; Diseases of mother, fetus and pregnancy ; Endothelial Cells - cytology ; endothelial progenitor cell ; Female ; Fetal Blood - cytology ; Fetus - cytology ; Flow Cytometry ; Gynecology. Andrology. Obstetrics ; Humans ; Medical sciences ; Obstetrics and Gynecology ; Pre-Eclampsia - pathology ; Pre-Eclampsia - physiopathology ; preeclampsia ; Pregnancy ; Pregnancy. Fetus. Placenta ; senescence</subject><ispartof>American journal of obstetrics and gynecology, 2008-09, Vol.199 (3), p.259.e1-259.e7</ispartof><rights>Mosby, Inc.</rights><rights>2008 Mosby, Inc.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-b1f1acb913574bdf943d01db822dadf9a7bf12907326f4aa82baaec7230c2b7d3</citedby><cites>FETCH-LOGICAL-c439t-b1f1acb913574bdf943d01db822dadf9a7bf12907326f4aa82baaec7230c2b7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0002937808006923$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20637529$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18771975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hwang, Han-Sung, MD</creatorcontrib><creatorcontrib>Maeng, Yong-Sun, MS</creatorcontrib><creatorcontrib>Park, Yong-Won, MD, PhD</creatorcontrib><creatorcontrib>Koos, Brian J., MD, PhD</creatorcontrib><creatorcontrib>Kwon, Young-Guen, PhD</creatorcontrib><creatorcontrib>Kim, Young-Han, MD, PhD</creatorcontrib><title>Increased senescence and reduced functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction</title><title>American journal of obstetrics and gynecology</title><addtitle>Am J Obstet Gynecol</addtitle><description>Objective The aim of this study was to investigate the number and functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction. Study Design Fetal endothelial progenitor cells were isolated, and counted from 17 women with preeclampsia without intrauterine growth restricion and 30 normal women. Colony-forming assay and differentiation time assay were performed to detect functional activity of the cells. To assess cellular senescence, senescence-associated β-galactosidase staining was performed for endothelial progenitor cells. Results Compared with normal pregnancy, the number of endothelial progenitor cells was significantly lower, differentiation time from endothelial progenitor cell into outgrowing cell was longer, and the number of colonies after differentiation was smaller in preeclampsia ( P < .001), respectively. The intensity of senescence-associated β-galactosidase staining was higher in preeclamptic pregnancy ( P < .001). Conclusion The number and functional ability of fetal endothelial progenitor cells from preeclampsia without intrauterine growth restriction are significantly decreased and they are more senescent compared with those of normal pregnancy.</description><subject>Adult</subject><subject>beta-Galactosidase - analysis</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Cellular Senescence</subject><subject>Colony-Forming Units Assay</subject><subject>Diseases of mother, fetus and pregnancy</subject><subject>Endothelial Cells - cytology</subject><subject>endothelial progenitor cell</subject><subject>Female</subject><subject>Fetal Blood - cytology</subject><subject>Fetus - cytology</subject><subject>Flow Cytometry</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Obstetrics and Gynecology</subject><subject>Pre-Eclampsia - pathology</subject><subject>Pre-Eclampsia - physiopathology</subject><subject>preeclampsia</subject><subject>Pregnancy</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>senescence</subject><issn>0002-9378</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uk2LFDEQbURx19U_4EFy0duM-ZhJOiDCsvixsOBBPYfqpHomYyYZk7TL_Cd_pGlnUPAgFCRVea9SVa-67jmjS0aZfL1bwi5tlpzSfkllM_qgu2RUq4XsZf-wu6SU8oUWqr_onpSym12u-ePugvVKMa3Wl93P22gzQkFHCkYsFqNFAtGRjG6yLTxO0VafIgQCgw--HkkayYi1BTC6VLcYfLsfctpg9DVlYjGEQnxsMdxEiNZjITbtD8FbqC3ncJyf0AbYH4oHcu_rNk21UWqGqWL2Eckmp_u6bXWUmv3vEp52j0YIBZ-dz6vu6_t3X24-Lu4-fbi9ub5b2JXQdTGwkYEdNBNrtRrcqFfCUeaGnnMHzQU1jIxrqgSX4wqg5wMAWsUFtXxQTlx1r055W0_fp_a_2fsyNwUR01SM1Gu2FlI2ID8BbU6lZBzNIfs95KNh1MwamZ2ZNTKzRobKZrSRXpyzT8Me3V_KWZQGeHkGQLEQxjxPsPzBcSqFWnPdcG9OOGyz-OExm9Im3fRzPqOtxiX__zre_kO3wcemUPiGRyy7NOUmejHMFG6o-Tyvz7xMtKdUai7ELwrIyys</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Hwang, Han-Sung, MD</creator><creator>Maeng, Yong-Sun, MS</creator><creator>Park, Yong-Won, MD, PhD</creator><creator>Koos, Brian J., MD, PhD</creator><creator>Kwon, Young-Guen, PhD</creator><creator>Kim, Young-Han, MD, PhD</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>Increased senescence and reduced functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction</title><author>Hwang, Han-Sung, MD ; Maeng, Yong-Sun, MS ; Park, Yong-Won, MD, PhD ; Koos, Brian J., MD, PhD ; Kwon, Young-Guen, PhD ; Kim, Young-Han, MD, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-b1f1acb913574bdf943d01db822dadf9a7bf12907326f4aa82baaec7230c2b7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>beta-Galactosidase - analysis</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Cellular Senescence</topic><topic>Colony-Forming Units Assay</topic><topic>Diseases of mother, fetus and pregnancy</topic><topic>Endothelial Cells - cytology</topic><topic>endothelial progenitor cell</topic><topic>Female</topic><topic>Fetal Blood - cytology</topic><topic>Fetus - cytology</topic><topic>Flow Cytometry</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Obstetrics and Gynecology</topic><topic>Pre-Eclampsia - pathology</topic><topic>Pre-Eclampsia - physiopathology</topic><topic>preeclampsia</topic><topic>Pregnancy</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>senescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hwang, Han-Sung, MD</creatorcontrib><creatorcontrib>Maeng, Yong-Sun, MS</creatorcontrib><creatorcontrib>Park, Yong-Won, MD, PhD</creatorcontrib><creatorcontrib>Koos, Brian J., MD, PhD</creatorcontrib><creatorcontrib>Kwon, Young-Guen, PhD</creatorcontrib><creatorcontrib>Kim, Young-Han, MD, PhD</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hwang, Han-Sung, MD</au><au>Maeng, Yong-Sun, MS</au><au>Park, Yong-Won, MD, PhD</au><au>Koos, Brian J., MD, PhD</au><au>Kwon, Young-Guen, PhD</au><au>Kim, Young-Han, MD, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased senescence and reduced functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>199</volume><issue>3</issue><spage>259.e1</spage><epage>259.e7</epage><pages>259.e1-259.e7</pages><issn>0002-9378</issn><eissn>1097-6868</eissn><coden>AJOGAH</coden><abstract>Objective The aim of this study was to investigate the number and functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction. Study Design Fetal endothelial progenitor cells were isolated, and counted from 17 women with preeclampsia without intrauterine growth restricion and 30 normal women. Colony-forming assay and differentiation time assay were performed to detect functional activity of the cells. To assess cellular senescence, senescence-associated β-galactosidase staining was performed for endothelial progenitor cells. Results Compared with normal pregnancy, the number of endothelial progenitor cells was significantly lower, differentiation time from endothelial progenitor cell into outgrowing cell was longer, and the number of colonies after differentiation was smaller in preeclampsia ( P < .001), respectively. The intensity of senescence-associated β-galactosidase staining was higher in preeclamptic pregnancy ( P < .001). Conclusion The number and functional ability of fetal endothelial progenitor cells from preeclampsia without intrauterine growth restriction are significantly decreased and they are more senescent compared with those of normal pregnancy.</abstract><cop>Philadelphia, PA</cop><pub>Mosby, Inc</pub><pmid>18771975</pmid><doi>10.1016/j.ajog.2008.06.060</doi><tpages>3</tpages></addata></record> |
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| subjects | Adult beta-Galactosidase - analysis Biological and medical sciences Cell Differentiation Cells, Cultured Cellular Senescence Colony-Forming Units Assay Diseases of mother, fetus and pregnancy Endothelial Cells - cytology endothelial progenitor cell Female Fetal Blood - cytology Fetus - cytology Flow Cytometry Gynecology. Andrology. Obstetrics Humans Medical sciences Obstetrics and Gynecology Pre-Eclampsia - pathology Pre-Eclampsia - physiopathology preeclampsia Pregnancy Pregnancy. Fetus. Placenta senescence |
| title | Increased senescence and reduced functional ability of fetal endothelial progenitor cells in pregnancies complicated by preeclampsia without intrauterine growth restriction |
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