Simultaneous detection and quantification of 3-nitrotyrosine and 3-bromotyrosine in human urine by stable isotope dilution liquid chromatography tandem mass spectrometry
Nitration and bromination of proteins, giving rise to the respective 3-nitrotyrosine (3NT) and 3-bromotyrosine (3BT), are implicated in asthma, allergic inflammatory disorders, and cancer. We have developed an isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry (L...
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| Veröffentlicht in: | Toxicology letters 2008-09, Vol.181 (1), p.31-39 |
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| creator | Chen, Hauh-Jyun Candy Chiu, Wei-Loong |
| description | Nitration and bromination of proteins, giving rise to the respective 3-nitrotyrosine (3NT) and 3-bromotyrosine (3BT), are implicated in asthma, allergic inflammatory disorders, and cancer. We have developed an isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry (LC/MS/MS) assay for simultaneous analysis of protein-bound 3NT and 3BT in human urine. The detection limits (S/N
=
3) were 10
pg (44
fmol) for 3NT and 5.0
pg (19
fmol) for 3BT injected on-column. The average levels of protein-bound 3NT and 3BT in 23 healthy individuals were 9.7
±
11.0 (mean
±
S.D.) in 10
5 tyrosine and 4.4
±
3.9 (mean
±
S.D.) in 10
3 tyrosine, respectively, using this highly sensitive LC/MS/MS under the selective reaction monitoring mode. Furthermore, the levels of urinary 3NT and 3BT show a statistically significant correlation (
R
2
=
0.55,
p
=
0.0065,
n
=
23). The high specificity and accuracy of this LC/MS/MS method render it a valuable tool in measurement of 3NT and 3BrT in the human urinary protein as promising noninvasive biomarkers for protein tyrosine nitration and bromination
in vivo. |
| doi_str_mv | 10.1016/j.toxlet.2008.06.867 |
| format | Article |
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=
3) were 10
pg (44
fmol) for 3NT and 5.0
pg (19
fmol) for 3BT injected on-column. The average levels of protein-bound 3NT and 3BT in 23 healthy individuals were 9.7
±
11.0 (mean
±
S.D.) in 10
5 tyrosine and 4.4
±
3.9 (mean
±
S.D.) in 10
3 tyrosine, respectively, using this highly sensitive LC/MS/MS under the selective reaction monitoring mode. Furthermore, the levels of urinary 3NT and 3BT show a statistically significant correlation (
R
2
=
0.55,
p
=
0.0065,
n
=
23). The high specificity and accuracy of this LC/MS/MS method render it a valuable tool in measurement of 3NT and 3BrT in the human urinary protein as promising noninvasive biomarkers for protein tyrosine nitration and bromination
in vivo.</description><identifier>ISSN: 0378-4274</identifier><identifier>EISSN: 1879-3169</identifier><identifier>DOI: 10.1016/j.toxlet.2008.06.867</identifier><identifier>PMID: 18652882</identifier><identifier>CODEN: TOLED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>3-Bromotyrosine ; 3-Nitrotyrosine ; Biological and medical sciences ; Calibration ; Chromatography, Liquid ; Human urine ; Humans ; Liquid chromatography mass spectrometry ; Medical sciences ; Nephrology. Urinary tract diseases ; Radioisotope Dilution Technique ; Sensitivity and Specificity ; Tandem Mass Spectrometry ; Toxicology ; Tyrosine - analogs & derivatives ; Tyrosine - urine ; Urinary protein ; Urinary system involvement in other diseases. Miscellaneous ; Urinary tract. Prostate gland</subject><ispartof>Toxicology letters, 2008-09, Vol.181 (1), p.31-39</ispartof><rights>2008 Elsevier Ireland Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-5bfd12ab214e0b78e12d547e5637e9a6521477471161c2ab2c758d26ef814ffa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.toxlet.2008.06.867$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20706967$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18652882$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Hauh-Jyun Candy</creatorcontrib><creatorcontrib>Chiu, Wei-Loong</creatorcontrib><title>Simultaneous detection and quantification of 3-nitrotyrosine and 3-bromotyrosine in human urine by stable isotope dilution liquid chromatography tandem mass spectrometry</title><title>Toxicology letters</title><addtitle>Toxicol Lett</addtitle><description>Nitration and bromination of proteins, giving rise to the respective 3-nitrotyrosine (3NT) and 3-bromotyrosine (3BT), are implicated in asthma, allergic inflammatory disorders, and cancer. We have developed an isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry (LC/MS/MS) assay for simultaneous analysis of protein-bound 3NT and 3BT in human urine. The detection limits (S/N
=
3) were 10
pg (44
fmol) for 3NT and 5.0
pg (19
fmol) for 3BT injected on-column. The average levels of protein-bound 3NT and 3BT in 23 healthy individuals were 9.7
±
11.0 (mean
±
S.D.) in 10
5 tyrosine and 4.4
±
3.9 (mean
±
S.D.) in 10
3 tyrosine, respectively, using this highly sensitive LC/MS/MS under the selective reaction monitoring mode. Furthermore, the levels of urinary 3NT and 3BT show a statistically significant correlation (
R
2
=
0.55,
p
=
0.0065,
n
=
23). The high specificity and accuracy of this LC/MS/MS method render it a valuable tool in measurement of 3NT and 3BrT in the human urinary protein as promising noninvasive biomarkers for protein tyrosine nitration and bromination
in vivo.</description><subject>3-Bromotyrosine</subject><subject>3-Nitrotyrosine</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Chromatography, Liquid</subject><subject>Human urine</subject><subject>Humans</subject><subject>Liquid chromatography mass spectrometry</subject><subject>Medical sciences</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Radioisotope Dilution Technique</subject><subject>Sensitivity and Specificity</subject><subject>Tandem Mass Spectrometry</subject><subject>Toxicology</subject><subject>Tyrosine - analogs & derivatives</subject><subject>Tyrosine - urine</subject><subject>Urinary protein</subject><subject>Urinary system involvement in other diseases. Miscellaneous</subject><subject>Urinary tract. Prostate gland</subject><issn>0378-4274</issn><issn>1879-3169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhiMEotvCGyDkC9wSbMexnQsSqqAgVeIAnC3HnrBeJXHWdirySLwl3s2K3uBkeeabXzP_XxSvCK4IJvzdoUr-1wCpohjLCvNKcvGk2BEp2rImvH1a7HAtZMmoYFfFdYwHjDFnvHleXBHJGyol3RW_v7lxGZKewC8RWUhgkvMT0pNFx0VPyfXO6HPJ96guJ5eCT2vw0U1wpuqyC358rLkJ7ZdRT2gJp2-3oph0N-RG9MnPgKwblrPg4I6Ls8js87xO_mfQ835FeRcLIxp1jCjOeZ3chRTWF8WzXg8RXl7em-LHp4_fbz-X91_vvtx-uC8NkyKVTddbQnVHCQPcCQmE2oYJaHgtoNX5bsKEYIIQTsyJM6KRlnLoJWF9r-ub4u2mOwd_XCAmNbpoYBg2jxRvWSslq_8LUtwyWXOSQbaBJlsUA_RqDm7UYVUEq1OW6qC2LNUpS4W5ylnmsdcX_aUbwT4OXcLLwJsLoKPRQx_0ZFz8y1EsMG_PQu83DrJtDw6CisbBZMC6kP1V1rt_b_IHRxLEhA</recordid><startdate>20080910</startdate><enddate>20080910</enddate><creator>Chen, Hauh-Jyun Candy</creator><creator>Chiu, Wei-Loong</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20080910</creationdate><title>Simultaneous detection and quantification of 3-nitrotyrosine and 3-bromotyrosine in human urine by stable isotope dilution liquid chromatography tandem mass spectrometry</title><author>Chen, Hauh-Jyun Candy ; Chiu, Wei-Loong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-5bfd12ab214e0b78e12d547e5637e9a6521477471161c2ab2c758d26ef814ffa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>3-Bromotyrosine</topic><topic>3-Nitrotyrosine</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Chromatography, Liquid</topic><topic>Human urine</topic><topic>Humans</topic><topic>Liquid chromatography mass spectrometry</topic><topic>Medical sciences</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Radioisotope Dilution Technique</topic><topic>Sensitivity and Specificity</topic><topic>Tandem Mass Spectrometry</topic><topic>Toxicology</topic><topic>Tyrosine - analogs & derivatives</topic><topic>Tyrosine - urine</topic><topic>Urinary protein</topic><topic>Urinary system involvement in other diseases. Miscellaneous</topic><topic>Urinary tract. Prostate gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Hauh-Jyun Candy</creatorcontrib><creatorcontrib>Chiu, Wei-Loong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Toxicology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Hauh-Jyun Candy</au><au>Chiu, Wei-Loong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous detection and quantification of 3-nitrotyrosine and 3-bromotyrosine in human urine by stable isotope dilution liquid chromatography tandem mass spectrometry</atitle><jtitle>Toxicology letters</jtitle><addtitle>Toxicol Lett</addtitle><date>2008-09-10</date><risdate>2008</risdate><volume>181</volume><issue>1</issue><spage>31</spage><epage>39</epage><pages>31-39</pages><issn>0378-4274</issn><eissn>1879-3169</eissn><coden>TOLED5</coden><abstract>Nitration and bromination of proteins, giving rise to the respective 3-nitrotyrosine (3NT) and 3-bromotyrosine (3BT), are implicated in asthma, allergic inflammatory disorders, and cancer. We have developed an isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry (LC/MS/MS) assay for simultaneous analysis of protein-bound 3NT and 3BT in human urine. The detection limits (S/N
=
3) were 10
pg (44
fmol) for 3NT and 5.0
pg (19
fmol) for 3BT injected on-column. The average levels of protein-bound 3NT and 3BT in 23 healthy individuals were 9.7
±
11.0 (mean
±
S.D.) in 10
5 tyrosine and 4.4
±
3.9 (mean
±
S.D.) in 10
3 tyrosine, respectively, using this highly sensitive LC/MS/MS under the selective reaction monitoring mode. Furthermore, the levels of urinary 3NT and 3BT show a statistically significant correlation (
R
2
=
0.55,
p
=
0.0065,
n
=
23). The high specificity and accuracy of this LC/MS/MS method render it a valuable tool in measurement of 3NT and 3BrT in the human urinary protein as promising noninvasive biomarkers for protein tyrosine nitration and bromination
in vivo.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>18652882</pmid><doi>10.1016/j.toxlet.2008.06.867</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-4274 |
| ispartof | Toxicology letters, 2008-09, Vol.181 (1), p.31-39 |
| issn | 0378-4274 1879-3169 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69498843 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 3-Bromotyrosine 3-Nitrotyrosine Biological and medical sciences Calibration Chromatography, Liquid Human urine Humans Liquid chromatography mass spectrometry Medical sciences Nephrology. Urinary tract diseases Radioisotope Dilution Technique Sensitivity and Specificity Tandem Mass Spectrometry Toxicology Tyrosine - analogs & derivatives Tyrosine - urine Urinary protein Urinary system involvement in other diseases. Miscellaneous Urinary tract. Prostate gland |
| title | Simultaneous detection and quantification of 3-nitrotyrosine and 3-bromotyrosine in human urine by stable isotope dilution liquid chromatography tandem mass spectrometry |
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