Effects of Physical Exercise on Liver ATP Levels in Fasted and Phosphate-Injected Rats
The purpose of the present study was to investigate the effects of exercise (30 min, 23 m/min, 0% grade) on the hepatic levels of ATP in fasted adrenodemedullated rats, with an intraperitoneal injection of sodium phosphate (Na 2 PO 4, 0.91 mM) or saline (NaCl). Sodium phosphate was injected to deter...
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| Veröffentlicht in: | Archives of physiology and biochemistry 1999-01, Vol.107 (5), p.393-402 |
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| description | The purpose of the present study was to investigate the effects of exercise (30 min, 23 m/min, 0% grade) on the hepatic levels of ATP in fasted adrenodemedullated rats, with an intraperitoneal injection of sodium phosphate (Na 2 PO 4, 0.91 mM) or saline (NaCl). Sodium phosphate was injected to determine if the postulated decrease in liver ATP during exercise may be changed by providing an excess of phosphate. At the end of exercise, a piece of liver was rapidly freeze clamped and used for the enzymatic determination of ATP levels. Liver ATP, in saline-injected rats, was significantly (P < 0.05) decreased by fasting, compared to fed rats (X ± SE: 3.21 ± 0.2 vs 2.86 ± 0.2 µmol/g). Exercise in fasted rats decreased even more the ATP response in liver (2.58 ± 0.14 µmol/g). Injection of Na 2 PO 4 did not significantly (P < 0.05) alter the pattern of ATP response following these 3 conditions (3.35 ± 0.14 vs 3.0 ± 0.12 vs 2.57 ± 0.1 µmol/g), ATP levels being significantly (P < 0.05) decreased by the fast and the exercise in the fasted state. Fasting and exercise resulted in a significant (P < 0.05) decrease in liver glycogen and plasma glucose concentrations and an increase in free fatty acid levels in both NaCl- and Na 2 PO 4 -injected groups. In both injection conditions, ß-hydroxybutyrate and peripheral insulin concentrations were respectively, increased and decreased (P < 0.05) by fasting, while norepinephrine and portal glucagon were decreased (P < 0.05) following exercise. The main effect of the injection of Na 2 PO 4 was a stimulation (P < 0.05) of peripheral glucagon response following exercise. It is concluded that exercise results in a decrease in liver ATP levels even in fasted rats and that this decrease is not corrected by Na 2 PO 4 administration. The decreased liver ATP levels might be involved in the metabolic adaptations to exercise. |
| doi_str_mv | 10.1076/138134551999121070515FT393 |
| format | Article |
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Sodium phosphate was injected to determine if the postulated decrease in liver ATP during exercise may be changed by providing an excess of phosphate. At the end of exercise, a piece of liver was rapidly freeze clamped and used for the enzymatic determination of ATP levels. Liver ATP, in saline-injected rats, was significantly (P < 0.05) decreased by fasting, compared to fed rats (X ± SE: 3.21 ± 0.2 vs 2.86 ± 0.2 µmol/g). Exercise in fasted rats decreased even more the ATP response in liver (2.58 ± 0.14 µmol/g). Injection of Na 2 PO 4 did not significantly (P < 0.05) alter the pattern of ATP response following these 3 conditions (3.35 ± 0.14 vs 3.0 ± 0.12 vs 2.57 ± 0.1 µmol/g), ATP levels being significantly (P < 0.05) decreased by the fast and the exercise in the fasted state. Fasting and exercise resulted in a significant (P < 0.05) decrease in liver glycogen and plasma glucose concentrations and an increase in free fatty acid levels in both NaCl- and Na 2 PO 4 -injected groups. In both injection conditions, ß-hydroxybutyrate and peripheral insulin concentrations were respectively, increased and decreased (P < 0.05) by fasting, while norepinephrine and portal glucagon were decreased (P < 0.05) following exercise. The main effect of the injection of Na 2 PO 4 was a stimulation (P < 0.05) of peripheral glucagon response following exercise. It is concluded that exercise results in a decrease in liver ATP levels even in fasted rats and that this decrease is not corrected by Na 2 PO 4 administration. The decreased liver ATP levels might be involved in the metabolic adaptations to exercise.]]></description><identifier>ISSN: 1381-3455</identifier><identifier>EISSN: 1744-4160</identifier><identifier>DOI: 10.1076/138134551999121070515FT393</identifier><identifier>PMID: 10916167</identifier><language>eng</language><publisher>Basingstoke: Informa UK Ltd</publisher><subject>Adenosine Triphosphate - metabolism ; Adrenal Medulla - surgery ; Animals ; Biological and medical sciences ; Blood Glucose - metabolism ; Chromatography, High Pressure Liquid ; Epinephrine - metabolism ; Fasting - metabolism ; Fundamental and applied biological sciences. Psychology ; Glucagon - metabolism ; Hepatic Veins ; Insulin - metabolism ; Lactic Acid - metabolism ; Liver - metabolism ; Liver Glycogen - metabolism ; Liver. Bile. Biliary tracts ; Male ; Norepinephrine - metabolism ; Phosphates - metabolism ; Phosphates - pharmacology ; Physical Conditioning, Animal - physiology ; Rats ; Rats, Sprague-Dawley ; Triglycerides - metabolism ; Vertebrates: digestive system</subject><ispartof>Archives of physiology and biochemistry, 1999-01, Vol.107 (5), p.393-402</ispartof><rights>1999 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1999</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-8075ce2972ef29a748f96684ebc35384120f8837a1465ed7792630d43e4fbb2e3</citedby><cites>FETCH-LOGICAL-c456t-8075ce2972ef29a748f96684ebc35384120f8837a1465ed7792630d43e4fbb2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1076/138134551999121070515FT393$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1076/138134551999121070515FT393$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1433138$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10916167$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ghanbari-Niaki, A.</creatorcontrib><creatorcontrib>Bergeron, R.</creatorcontrib><creatorcontrib>Latour, M.G.</creatorcontrib><creatorcontrib>Lavoie, J.M.</creatorcontrib><title>Effects of Physical Exercise on Liver ATP Levels in Fasted and Phosphate-Injected Rats</title><title>Archives of physiology and biochemistry</title><addtitle>Arch Physiol Biochem</addtitle><description><![CDATA[The purpose of the present study was to investigate the effects of exercise (30 min, 23 m/min, 0% grade) on the hepatic levels of ATP in fasted adrenodemedullated rats, with an intraperitoneal injection of sodium phosphate (Na 2 PO 4, 0.91 mM) or saline (NaCl). Sodium phosphate was injected to determine if the postulated decrease in liver ATP during exercise may be changed by providing an excess of phosphate. At the end of exercise, a piece of liver was rapidly freeze clamped and used for the enzymatic determination of ATP levels. Liver ATP, in saline-injected rats, was significantly (P < 0.05) decreased by fasting, compared to fed rats (X ± SE: 3.21 ± 0.2 vs 2.86 ± 0.2 µmol/g). Exercise in fasted rats decreased even more the ATP response in liver (2.58 ± 0.14 µmol/g). Injection of Na 2 PO 4 did not significantly (P < 0.05) alter the pattern of ATP response following these 3 conditions (3.35 ± 0.14 vs 3.0 ± 0.12 vs 2.57 ± 0.1 µmol/g), ATP levels being significantly (P < 0.05) decreased by the fast and the exercise in the fasted state. Fasting and exercise resulted in a significant (P < 0.05) decrease in liver glycogen and plasma glucose concentrations and an increase in free fatty acid levels in both NaCl- and Na 2 PO 4 -injected groups. In both injection conditions, ß-hydroxybutyrate and peripheral insulin concentrations were respectively, increased and decreased (P < 0.05) by fasting, while norepinephrine and portal glucagon were decreased (P < 0.05) following exercise. The main effect of the injection of Na 2 PO 4 was a stimulation (P < 0.05) of peripheral glucagon response following exercise. It is concluded that exercise results in a decrease in liver ATP levels even in fasted rats and that this decrease is not corrected by Na 2 PO 4 administration. The decreased liver ATP levels might be involved in the metabolic adaptations to exercise.]]></description><subject>Adenosine Triphosphate - metabolism</subject><subject>Adrenal Medulla - surgery</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - metabolism</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Epinephrine - metabolism</subject><subject>Fasting - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucagon - metabolism</subject><subject>Hepatic Veins</subject><subject>Insulin - metabolism</subject><subject>Lactic Acid - metabolism</subject><subject>Liver - metabolism</subject><subject>Liver Glycogen - metabolism</subject><subject>Liver. Bile. Biliary tracts</subject><subject>Male</subject><subject>Norepinephrine - metabolism</subject><subject>Phosphates - metabolism</subject><subject>Phosphates - pharmacology</subject><subject>Physical Conditioning, Animal - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Triglycerides - metabolism</subject><subject>Vertebrates: digestive system</subject><issn>1381-3455</issn><issn>1744-4160</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1qGzEURkVJaNK0r1BECdlNozvS6Ke7kNiJwRAT3G4HWXOFx4xHrjRO4revgl3aLAJZSVzO9-nqEPIN2HdgSl4C18BFVYExBso8YhVU4zk3_AM5BSVEIUCyo3zPYPFCnpBPKa0Yg1Ib-EhOgBmQINUp-TXyHt2QaPB0ttyl1tmOjp4xujYhDT2dto8Y6dV8Rqf4iF2ibU_HNg3YUNs3ORPSZmkHLCb9Khfl8YMd0mdy7G2X8MvhPCM_x6P59V0xvb-dXF9NCycqORSaqcphaVSJvjRWCe2NlFrgwvGKawEl81pzZUHIChulTCk5awRH4ReLEvkZudj3bmL4vcU01Os2Oew622PYploaYTRolsEfe9DFkFJEX29iu7ZxVwOrX6zWb1vN4a-HV7aLNTb_RfcaM3B-AGzKAn20ffb3jxOc5_qMjfZY2_sQ1_YpxK6pB7vrQvyb4e_a5-ZVzxJtNyydjVivwjb22fh7vvUHpUenNA</recordid><startdate>19990101</startdate><enddate>19990101</enddate><creator>Ghanbari-Niaki, A.</creator><creator>Bergeron, R.</creator><creator>Latour, M.G.</creator><creator>Lavoie, J.M.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990101</creationdate><title>Effects of Physical Exercise on Liver ATP Levels in Fasted and Phosphate-Injected Rats</title><author>Ghanbari-Niaki, A. ; Bergeron, R. ; Latour, M.G. ; Lavoie, J.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-8075ce2972ef29a748f96684ebc35384120f8837a1465ed7792630d43e4fbb2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Adrenal Medulla - surgery</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - metabolism</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Epinephrine - metabolism</topic><topic>Fasting - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucagon - metabolism</topic><topic>Hepatic Veins</topic><topic>Insulin - metabolism</topic><topic>Lactic Acid - metabolism</topic><topic>Liver - metabolism</topic><topic>Liver Glycogen - metabolism</topic><topic>Liver. Bile. Biliary tracts</topic><topic>Male</topic><topic>Norepinephrine - metabolism</topic><topic>Phosphates - metabolism</topic><topic>Phosphates - pharmacology</topic><topic>Physical Conditioning, Animal - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Triglycerides - metabolism</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghanbari-Niaki, A.</creatorcontrib><creatorcontrib>Bergeron, R.</creatorcontrib><creatorcontrib>Latour, M.G.</creatorcontrib><creatorcontrib>Lavoie, J.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghanbari-Niaki, A.</au><au>Bergeron, R.</au><au>Latour, M.G.</au><au>Lavoie, J.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Physical Exercise on Liver ATP Levels in Fasted and Phosphate-Injected Rats</atitle><jtitle>Archives of physiology and biochemistry</jtitle><addtitle>Arch Physiol Biochem</addtitle><date>1999-01-01</date><risdate>1999</risdate><volume>107</volume><issue>5</issue><spage>393</spage><epage>402</epage><pages>393-402</pages><issn>1381-3455</issn><eissn>1744-4160</eissn><abstract><![CDATA[The purpose of the present study was to investigate the effects of exercise (30 min, 23 m/min, 0% grade) on the hepatic levels of ATP in fasted adrenodemedullated rats, with an intraperitoneal injection of sodium phosphate (Na 2 PO 4, 0.91 mM) or saline (NaCl). Sodium phosphate was injected to determine if the postulated decrease in liver ATP during exercise may be changed by providing an excess of phosphate. At the end of exercise, a piece of liver was rapidly freeze clamped and used for the enzymatic determination of ATP levels. Liver ATP, in saline-injected rats, was significantly (P < 0.05) decreased by fasting, compared to fed rats (X ± SE: 3.21 ± 0.2 vs 2.86 ± 0.2 µmol/g). Exercise in fasted rats decreased even more the ATP response in liver (2.58 ± 0.14 µmol/g). Injection of Na 2 PO 4 did not significantly (P < 0.05) alter the pattern of ATP response following these 3 conditions (3.35 ± 0.14 vs 3.0 ± 0.12 vs 2.57 ± 0.1 µmol/g), ATP levels being significantly (P < 0.05) decreased by the fast and the exercise in the fasted state. Fasting and exercise resulted in a significant (P < 0.05) decrease in liver glycogen and plasma glucose concentrations and an increase in free fatty acid levels in both NaCl- and Na 2 PO 4 -injected groups. In both injection conditions, ß-hydroxybutyrate and peripheral insulin concentrations were respectively, increased and decreased (P < 0.05) by fasting, while norepinephrine and portal glucagon were decreased (P < 0.05) following exercise. The main effect of the injection of Na 2 PO 4 was a stimulation (P < 0.05) of peripheral glucagon response following exercise. It is concluded that exercise results in a decrease in liver ATP levels even in fasted rats and that this decrease is not corrected by Na 2 PO 4 administration. The decreased liver ATP levels might be involved in the metabolic adaptations to exercise.]]></abstract><cop>Basingstoke</cop><pub>Informa UK Ltd</pub><pmid>10916167</pmid><doi>10.1076/138134551999121070515FT393</doi><tpages>10</tpages></addata></record> |
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| subjects | Adenosine Triphosphate - metabolism Adrenal Medulla - surgery Animals Biological and medical sciences Blood Glucose - metabolism Chromatography, High Pressure Liquid Epinephrine - metabolism Fasting - metabolism Fundamental and applied biological sciences. Psychology Glucagon - metabolism Hepatic Veins Insulin - metabolism Lactic Acid - metabolism Liver - metabolism Liver Glycogen - metabolism Liver. Bile. Biliary tracts Male Norepinephrine - metabolism Phosphates - metabolism Phosphates - pharmacology Physical Conditioning, Animal - physiology Rats Rats, Sprague-Dawley Triglycerides - metabolism Vertebrates: digestive system |
| title | Effects of Physical Exercise on Liver ATP Levels in Fasted and Phosphate-Injected Rats |
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