Expression of effector gene SIX1 of Fusarium oxysporum requires living plant cells
Fusarium oxysporum is an asexual, soil inhabiting fungus that comprises many different formae speciales, each pathogenic towards a different host plant. In absence of a suitable host all F. oxysporum isolates appear to have a very similar lifestyle, feeding on plant debris and colonizing the rhizosp...
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| Veröffentlicht in: | Fungal genetics and biology 2008-09, Vol.45 (9), p.1257-1264 |
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| container_title | Fungal genetics and biology |
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| creator | van der Does, H. Charlotte Duyvesteijn, Roselinde G.E. Goltstein, Pieter M. van Schie, Chris C.N. Manders, Erik M.M. Cornelissen, Ben J.C. Rep, Martijn |
| description | Fusarium oxysporum is an asexual, soil inhabiting fungus that comprises many different
formae speciales, each pathogenic towards a different host plant. In absence of a suitable host all
F. oxysporum isolates appear to have a very similar lifestyle, feeding on plant debris and colonizing the rhizosphere of living plants. Upon infection
F. oxysporum switches from a saprophytic to an infectious lifestyle, which probably includes the reprogramming of gene expression. In this work we show that the expression of the known effector gene
SIX1 of
F. oxysporum f. sp.
lycopersici is strongly upregulated during colonization of the host plant. Using GFP (green fluorescent protein) as reporter, we show that induction of
SIX1 expression starts immediately upon penetration of the root cortex. Induction requires living plant cells, but is not host specific and does not depend on morphological features of roots, since plant cells in culture can also induce
SIX1 expression. Taken together,
F. oxysporum seems to be able to distinguish between living and dead plant material, preventing unnecessary switches from a saprophytic to an infectious lifestyle. |
| doi_str_mv | 10.1016/j.fgb.2008.06.002 |
| format | Article |
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formae speciales, each pathogenic towards a different host plant. In absence of a suitable host all
F. oxysporum isolates appear to have a very similar lifestyle, feeding on plant debris and colonizing the rhizosphere of living plants. Upon infection
F. oxysporum switches from a saprophytic to an infectious lifestyle, which probably includes the reprogramming of gene expression. In this work we show that the expression of the known effector gene
SIX1 of
F. oxysporum f. sp.
lycopersici is strongly upregulated during colonization of the host plant. Using GFP (green fluorescent protein) as reporter, we show that induction of
SIX1 expression starts immediately upon penetration of the root cortex. Induction requires living plant cells, but is not host specific and does not depend on morphological features of roots, since plant cells in culture can also induce
SIX1 expression. Taken together,
F. oxysporum seems to be able to distinguish between living and dead plant material, preventing unnecessary switches from a saprophytic to an infectious lifestyle.</description><identifier>ISSN: 1087-1845</identifier><identifier>EISSN: 1096-0937</identifier><identifier>DOI: 10.1016/j.fgb.2008.06.002</identifier><identifier>PMID: 18606236</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Avirulence ; Cells, Cultured ; Confocal microscopy ; cultured cells ; dead plant material ; effector genes ; fungal proteins ; Fungal Proteins - analysis ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Fusarium - chemistry ; Fusarium - genetics ; Fusarium - metabolism ; Fusarium oxysporum ; gene expression ; gene expression regulation ; Gene Expression Regulation, Fungal ; Green fluorescent protein ; Green Fluorescent Proteins - analysis ; Green Fluorescent Proteins - genetics ; Green Fluorescent Proteins - metabolism ; host plants ; infection ; living plant material ; Lycopersicon esculentum - microbiology ; microbial colonization ; pathogenicity ; Plant Diseases - microbiology ; plant morphology ; plant pathogenic fungi ; Plant Roots - microbiology ; protein synthesis ; Recombinant Fusion Proteins - analysis ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; rhizosphere ; Root colonization ; Tomato ; Two-photon microscopy ; Xylem - chemistry ; Xylem - genetics ; Xylem - metabolism</subject><ispartof>Fungal genetics and biology, 2008-09, Vol.45 (9), p.1257-1264</ispartof><rights>2008 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c515t-e97b2172855cf3a193eb049e074238623aaa25a17ec409882f2c065213a2e5433</citedby><cites>FETCH-LOGICAL-c515t-e97b2172855cf3a193eb049e074238623aaa25a17ec409882f2c065213a2e5433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1087184508001047$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18606236$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van der Does, H. Charlotte</creatorcontrib><creatorcontrib>Duyvesteijn, Roselinde G.E.</creatorcontrib><creatorcontrib>Goltstein, Pieter M.</creatorcontrib><creatorcontrib>van Schie, Chris C.N.</creatorcontrib><creatorcontrib>Manders, Erik M.M.</creatorcontrib><creatorcontrib>Cornelissen, Ben J.C.</creatorcontrib><creatorcontrib>Rep, Martijn</creatorcontrib><title>Expression of effector gene SIX1 of Fusarium oxysporum requires living plant cells</title><title>Fungal genetics and biology</title><addtitle>Fungal Genet Biol</addtitle><description>Fusarium oxysporum is an asexual, soil inhabiting fungus that comprises many different
formae speciales, each pathogenic towards a different host plant. In absence of a suitable host all
F. oxysporum isolates appear to have a very similar lifestyle, feeding on plant debris and colonizing the rhizosphere of living plants. Upon infection
F. oxysporum switches from a saprophytic to an infectious lifestyle, which probably includes the reprogramming of gene expression. In this work we show that the expression of the known effector gene
SIX1 of
F. oxysporum f. sp.
lycopersici is strongly upregulated during colonization of the host plant. Using GFP (green fluorescent protein) as reporter, we show that induction of
SIX1 expression starts immediately upon penetration of the root cortex. Induction requires living plant cells, but is not host specific and does not depend on morphological features of roots, since plant cells in culture can also induce
SIX1 expression. Taken together,
F. oxysporum seems to be able to distinguish between living and dead plant material, preventing unnecessary switches from a saprophytic to an infectious lifestyle.</description><subject>Avirulence</subject><subject>Cells, Cultured</subject><subject>Confocal microscopy</subject><subject>cultured cells</subject><subject>dead plant material</subject><subject>effector genes</subject><subject>fungal proteins</subject><subject>Fungal Proteins - analysis</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Fusarium - chemistry</subject><subject>Fusarium - genetics</subject><subject>Fusarium - metabolism</subject><subject>Fusarium oxysporum</subject><subject>gene expression</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Green fluorescent protein</subject><subject>Green Fluorescent Proteins - analysis</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>host plants</subject><subject>infection</subject><subject>living plant material</subject><subject>Lycopersicon esculentum - microbiology</subject><subject>microbial colonization</subject><subject>pathogenicity</subject><subject>Plant Diseases - microbiology</subject><subject>plant morphology</subject><subject>plant pathogenic fungi</subject><subject>Plant Roots - microbiology</subject><subject>protein synthesis</subject><subject>Recombinant Fusion Proteins - analysis</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>rhizosphere</subject><subject>Root colonization</subject><subject>Tomato</subject><subject>Two-photon microscopy</subject><subject>Xylem - chemistry</subject><subject>Xylem - genetics</subject><subject>Xylem - metabolism</subject><issn>1087-1845</issn><issn>1096-0937</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi0EoqXwAFwgJ24JM3bsxOKEqhYqVUKiVOJmeb3jlVfZOLWTqn17HO1K3ODkkfX9v2Y-xt4jNAioPu8bv9s0HKBvQDUA_AU7R9CqBi26l-vcdzX2rTxjb3LeAyDKFl-zM-wVKC7UOft59TQlyjnEsYq-Iu_JzTFVOxqpurv5jevv9ZJtCsuhik_PeYqpTIkellCC1RAew7irpsGOc-VoGPJb9srbIdO703vB7q-vfl1-r29_fLu5_HpbO4lyrkl3G44d76V0XljUgjbQaoKu5aIv21lrubTYkWtB9z333IGSHIXlJFshLtinY--U4sNCeTaHkNcN7EhxyUbpVqtWdP8FOei2NEMB8Qi6FHNO5M2UwsGmZ4NgVuNmb4pxsxo3oEwxXjIfTuXL5kDbv4mT4gJ8PALeRmN3KWRzf8cBBaBUsu_WQ74cCSq2HgMlk12g0dG2GHaz2cbwjwX-AHzlmM0</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>van der Does, H. Charlotte</creator><creator>Duyvesteijn, Roselinde G.E.</creator><creator>Goltstein, Pieter M.</creator><creator>van Schie, Chris C.N.</creator><creator>Manders, Erik M.M.</creator><creator>Cornelissen, Ben J.C.</creator><creator>Rep, Martijn</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>Expression of effector gene SIX1 of Fusarium oxysporum requires living plant cells</title><author>van der Does, H. Charlotte ; Duyvesteijn, Roselinde G.E. ; Goltstein, Pieter M. ; van Schie, Chris C.N. ; Manders, Erik M.M. ; Cornelissen, Ben J.C. ; Rep, Martijn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c515t-e97b2172855cf3a193eb049e074238623aaa25a17ec409882f2c065213a2e5433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Avirulence</topic><topic>Cells, Cultured</topic><topic>Confocal microscopy</topic><topic>cultured cells</topic><topic>dead plant material</topic><topic>effector genes</topic><topic>fungal proteins</topic><topic>Fungal Proteins - analysis</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Fusarium - chemistry</topic><topic>Fusarium - genetics</topic><topic>Fusarium - metabolism</topic><topic>Fusarium oxysporum</topic><topic>gene expression</topic><topic>gene expression regulation</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Green fluorescent protein</topic><topic>Green Fluorescent Proteins - analysis</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>host plants</topic><topic>infection</topic><topic>living plant material</topic><topic>Lycopersicon esculentum - microbiology</topic><topic>microbial colonization</topic><topic>pathogenicity</topic><topic>Plant Diseases - microbiology</topic><topic>plant morphology</topic><topic>plant pathogenic fungi</topic><topic>Plant Roots - microbiology</topic><topic>protein synthesis</topic><topic>Recombinant Fusion Proteins - analysis</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>rhizosphere</topic><topic>Root colonization</topic><topic>Tomato</topic><topic>Two-photon microscopy</topic><topic>Xylem - chemistry</topic><topic>Xylem - genetics</topic><topic>Xylem - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van der Does, H. Charlotte</creatorcontrib><creatorcontrib>Duyvesteijn, Roselinde G.E.</creatorcontrib><creatorcontrib>Goltstein, Pieter M.</creatorcontrib><creatorcontrib>van Schie, Chris C.N.</creatorcontrib><creatorcontrib>Manders, Erik M.M.</creatorcontrib><creatorcontrib>Cornelissen, Ben J.C.</creatorcontrib><creatorcontrib>Rep, Martijn</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Fungal genetics and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van der Does, H. Charlotte</au><au>Duyvesteijn, Roselinde G.E.</au><au>Goltstein, Pieter M.</au><au>van Schie, Chris C.N.</au><au>Manders, Erik M.M.</au><au>Cornelissen, Ben J.C.</au><au>Rep, Martijn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of effector gene SIX1 of Fusarium oxysporum requires living plant cells</atitle><jtitle>Fungal genetics and biology</jtitle><addtitle>Fungal Genet Biol</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>45</volume><issue>9</issue><spage>1257</spage><epage>1264</epage><pages>1257-1264</pages><issn>1087-1845</issn><eissn>1096-0937</eissn><abstract>Fusarium oxysporum is an asexual, soil inhabiting fungus that comprises many different
formae speciales, each pathogenic towards a different host plant. In absence of a suitable host all
F. oxysporum isolates appear to have a very similar lifestyle, feeding on plant debris and colonizing the rhizosphere of living plants. Upon infection
F. oxysporum switches from a saprophytic to an infectious lifestyle, which probably includes the reprogramming of gene expression. In this work we show that the expression of the known effector gene
SIX1 of
F. oxysporum f. sp.
lycopersici is strongly upregulated during colonization of the host plant. Using GFP (green fluorescent protein) as reporter, we show that induction of
SIX1 expression starts immediately upon penetration of the root cortex. Induction requires living plant cells, but is not host specific and does not depend on morphological features of roots, since plant cells in culture can also induce
SIX1 expression. Taken together,
F. oxysporum seems to be able to distinguish between living and dead plant material, preventing unnecessary switches from a saprophytic to an infectious lifestyle.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>18606236</pmid><doi>10.1016/j.fgb.2008.06.002</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1087-1845 |
| ispartof | Fungal genetics and biology, 2008-09, Vol.45 (9), p.1257-1264 |
| issn | 1087-1845 1096-0937 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Avirulence Cells, Cultured Confocal microscopy cultured cells dead plant material effector genes fungal proteins Fungal Proteins - analysis Fungal Proteins - genetics Fungal Proteins - metabolism Fusarium - chemistry Fusarium - genetics Fusarium - metabolism Fusarium oxysporum gene expression gene expression regulation Gene Expression Regulation, Fungal Green fluorescent protein Green Fluorescent Proteins - analysis Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism host plants infection living plant material Lycopersicon esculentum - microbiology microbial colonization pathogenicity Plant Diseases - microbiology plant morphology plant pathogenic fungi Plant Roots - microbiology protein synthesis Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism rhizosphere Root colonization Tomato Two-photon microscopy Xylem - chemistry Xylem - genetics Xylem - metabolism |
| title | Expression of effector gene SIX1 of Fusarium oxysporum requires living plant cells |
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