Evaluation of Brucella melitensis B115 as rough-phenotype vaccine against B. melitensis and B. ovis infections
Abstract Brucella melitensis strain Rev1 is used as vaccine for the prophylaxis of brucellosis in sheep and goats. Because of its smooth phenotype, however, it induces antibodies directed to the O-polysaccharide (O-PS) of the lipopolysaccharide (LPS), thus unabling to distinguish between vaccinated...
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| Veröffentlicht in: | Vaccine 2008-09, Vol.26 (38), p.4913-4917 |
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| description | Abstract Brucella melitensis strain Rev1 is used as vaccine for the prophylaxis of brucellosis in sheep and goats. Because of its smooth phenotype, however, it induces antibodies directed to the O-polysaccharide (O-PS) of the lipopolysaccharide (LPS), thus unabling to distinguish between vaccinated and infected animals. It has been speculated that alternative vaccines could be live, attenuated Brucella rough strains, which are devoid of the O-PS. B. melitensis B115 is a natural, attenuated, rough strain. The O-PS is not exposed at the surface but is present in the cytoplasm. We tested the protective activity of B115 against B. melitensis and B. ovis infections in mice, in comparison with that of Rev1. The residual virulence and the humoral response following B115 vaccination were also evaluated. Vaccination with B115 conferred significant protective immunity against B. melitensis 16M and B. ovis challenge strains, equivalent to that provided by Rev1. No interfering antibodies to O-PS were detected, while the B115 vaccination was monitored by a specific B115-based complement fixation test. These promising features suggest further evaluation of B. melitensis B115 as vaccine for target animal hosts. |
| doi_str_mv | 10.1016/j.vaccine.2008.07.030 |
| format | Article |
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Because of its smooth phenotype, however, it induces antibodies directed to the O-polysaccharide (O-PS) of the lipopolysaccharide (LPS), thus unabling to distinguish between vaccinated and infected animals. It has been speculated that alternative vaccines could be live, attenuated Brucella rough strains, which are devoid of the O-PS. B. melitensis B115 is a natural, attenuated, rough strain. The O-PS is not exposed at the surface but is present in the cytoplasm. We tested the protective activity of B115 against B. melitensis and B. ovis infections in mice, in comparison with that of Rev1. The residual virulence and the humoral response following B115 vaccination were also evaluated. Vaccination with B115 conferred significant protective immunity against B. melitensis 16M and B. ovis challenge strains, equivalent to that provided by Rev1. No interfering antibodies to O-PS were detected, while the B115 vaccination was monitored by a specific B115-based complement fixation test. These promising features suggest further evaluation of B. melitensis B115 as vaccine for target animal hosts.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2008.07.030</identifier><identifier>PMID: 18675869</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Allergy and Immunology ; Animals ; Antibody Formation ; Applied microbiology ; Bacteriology ; Biological and medical sciences ; Brucella melitensis ; Brucella melitensis - immunology ; Brucella ovis - immunology ; Brucella spp ; Brucella Vaccine - immunology ; Brucellosis - immunology ; Brucellosis - prevention & control ; Female ; Fundamental and applied biological sciences. Psychology ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Ovis ; Phenotype ; Rough-phenotype ; Spleen - immunology ; Spleen - microbiology ; Time Factors ; Vaccine ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><ispartof>Vaccine, 2008-09, Vol.26 (38), p.4913-4917</ispartof><rights>Elsevier Ltd</rights><rights>2008 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-fc8dc3ce026560844d513c54228c4b1609fe07770fb744e5ec1738f0d5524c03</citedby><cites>FETCH-LOGICAL-c479t-fc8dc3ce026560844d513c54228c4b1609fe07770fb744e5ec1738f0d5524c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vaccine.2008.07.030$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64387</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20665097$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18675869$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adone, R</creatorcontrib><creatorcontrib>Francia, M</creatorcontrib><creatorcontrib>Ciuchini, F</creatorcontrib><title>Evaluation of Brucella melitensis B115 as rough-phenotype vaccine against B. melitensis and B. ovis infections</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract Brucella melitensis strain Rev1 is used as vaccine for the prophylaxis of brucellosis in sheep and goats. Because of its smooth phenotype, however, it induces antibodies directed to the O-polysaccharide (O-PS) of the lipopolysaccharide (LPS), thus unabling to distinguish between vaccinated and infected animals. It has been speculated that alternative vaccines could be live, attenuated Brucella rough strains, which are devoid of the O-PS. B. melitensis B115 is a natural, attenuated, rough strain. The O-PS is not exposed at the surface but is present in the cytoplasm. We tested the protective activity of B115 against B. melitensis and B. ovis infections in mice, in comparison with that of Rev1. The residual virulence and the humoral response following B115 vaccination were also evaluated. Vaccination with B115 conferred significant protective immunity against B. melitensis 16M and B. ovis challenge strains, equivalent to that provided by Rev1. No interfering antibodies to O-PS were detected, while the B115 vaccination was monitored by a specific B115-based complement fixation test. These promising features suggest further evaluation of B. melitensis B115 as vaccine for target animal hosts.</description><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Antibody Formation</subject><subject>Applied microbiology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Brucella melitensis</subject><subject>Brucella melitensis - immunology</subject><subject>Brucella ovis - immunology</subject><subject>Brucella spp</subject><subject>Brucella Vaccine - immunology</subject><subject>Brucellosis - immunology</subject><subject>Brucellosis - prevention & control</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Ovis</subject><subject>Phenotype</subject><subject>Rough-phenotype</subject><subject>Spleen - immunology</subject><subject>Spleen - microbiology</subject><subject>Time Factors</subject><subject>Vaccine</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhi0EokvhJ4BygVvC2PFXLiC2KlCpUg_tgZvldSatl6yz2MlK--9x2AhQLz15ZD0z78y8Q8hbChUFKj9uq4N1zgesGICuQFVQwzOyolrVJRNUPycrYJKXnMKPM_IqpS0AiJo2L8kZ1VIJLZsVCZcH20929EMohq5Yx8lh39tih70fMSSfijWlorCpiMN0_1DuHzAM43GPxaJf2HvrQxqLdfV_lg3t_DMccuxDh26WSK_Ji872Cd8s7zm5-3p5d_G9vL75dnXx5bp0XDVj2TndutphHkBI0Jy3gtZOcMa04xsqoekQlFLQbRTnKNBRVesOWiEYd1Cfkw-nsvs4_JowjWbn05_BAg5TMrLhumkoexJkFKRWTZNBcQJdHFKK2Jl99Dsbj4aCmQ0xW7MsxMyGGFAmG5Lz3i0C02aH7b-sxYEMvF8Am5ztu2iD8-kvx0BKAY3K3OcTh3ltB4_RJOcxOGx9zMs17eCfbOXTowqu98Fn0Z94xLQdphiyJ4aaxAyY2_l65uMBDbkBzurfl_vAKA</recordid><startdate>20080908</startdate><enddate>20080908</enddate><creator>Adone, R</creator><creator>Francia, M</creator><creator>Ciuchini, F</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20080908</creationdate><title>Evaluation of Brucella melitensis B115 as rough-phenotype vaccine against B. melitensis and B. ovis infections</title><author>Adone, R ; Francia, M ; Ciuchini, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-fc8dc3ce026560844d513c54228c4b1609fe07770fb744e5ec1738f0d5524c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Antibody Formation</topic><topic>Applied microbiology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Brucella melitensis</topic><topic>Brucella melitensis - immunology</topic><topic>Brucella ovis - immunology</topic><topic>Brucella spp</topic><topic>Brucella Vaccine - immunology</topic><topic>Brucellosis - immunology</topic><topic>Brucellosis - prevention & control</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Ovis</topic><topic>Phenotype</topic><topic>Rough-phenotype</topic><topic>Spleen - immunology</topic><topic>Spleen - microbiology</topic><topic>Time Factors</topic><topic>Vaccine</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adone, R</creatorcontrib><creatorcontrib>Francia, M</creatorcontrib><creatorcontrib>Ciuchini, F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adone, R</au><au>Francia, M</au><au>Ciuchini, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Brucella melitensis B115 as rough-phenotype vaccine against B. melitensis and B. ovis infections</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2008-09-08</date><risdate>2008</risdate><volume>26</volume><issue>38</issue><spage>4913</spage><epage>4917</epage><pages>4913-4917</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract Brucella melitensis strain Rev1 is used as vaccine for the prophylaxis of brucellosis in sheep and goats. Because of its smooth phenotype, however, it induces antibodies directed to the O-polysaccharide (O-PS) of the lipopolysaccharide (LPS), thus unabling to distinguish between vaccinated and infected animals. It has been speculated that alternative vaccines could be live, attenuated Brucella rough strains, which are devoid of the O-PS. B. melitensis B115 is a natural, attenuated, rough strain. The O-PS is not exposed at the surface but is present in the cytoplasm. We tested the protective activity of B115 against B. melitensis and B. ovis infections in mice, in comparison with that of Rev1. The residual virulence and the humoral response following B115 vaccination were also evaluated. Vaccination with B115 conferred significant protective immunity against B. melitensis 16M and B. ovis challenge strains, equivalent to that provided by Rev1. No interfering antibodies to O-PS were detected, while the B115 vaccination was monitored by a specific B115-based complement fixation test. These promising features suggest further evaluation of B. melitensis B115 as vaccine for target animal hosts.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>18675869</pmid><doi>10.1016/j.vaccine.2008.07.030</doi><tpages>5</tpages></addata></record> |
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| subjects | Allergy and Immunology Animals Antibody Formation Applied microbiology Bacteriology Biological and medical sciences Brucella melitensis Brucella melitensis - immunology Brucella ovis - immunology Brucella spp Brucella Vaccine - immunology Brucellosis - immunology Brucellosis - prevention & control Female Fundamental and applied biological sciences. Psychology Mice Mice, Inbred BALB C Microbiology Miscellaneous Ovis Phenotype Rough-phenotype Spleen - immunology Spleen - microbiology Time Factors Vaccine Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) |
| title | Evaluation of Brucella melitensis B115 as rough-phenotype vaccine against B. melitensis and B. ovis infections |
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