A survey of genes in Eimeria tenella merozoites by EST sequencing
A study of about 500 expressed sequence tags (ESTs), derived from a merozoite cDNA library, was initiated as an approach to generate a larger pool of gene information on Eimeria tenella. Of the ESTs, 47.7% had matches with entries in the databases, including ribosomal proteins, metabolic enzymes and...
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| Veröffentlicht in: | International journal for parasitology 1999-12, Vol.29 (12), p.1885-1892 |
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| container_issue | 12 |
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| container_title | International journal for parasitology |
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| creator | Wan, Kiew-Lian Chong, Saw-Peng Ng, Szu-Ting Shirley, Martin W M.Tomley, Fiona Sanusi Jangi, Mohd |
| description | A study of about 500 expressed sequence tags (ESTs), derived from a merozoite cDNA library, was initiated as an approach to generate a larger pool of gene information on
Eimeria tenella. Of the ESTs, 47.7% had matches with entries in the databases, including ribosomal proteins, metabolic enzymes and proteins with other functions, of which 14.3% represented previously known
E. tenella genes. Thus over 50% of the ESTs had no significant database matches. The
E.
tenella EST dataset contained a range of highly abundant genes comparable with that found in the EST dataset of
T. gondii and may thus reflect the importance of such molecules in the biology of the apicomplexan organisms. However, comparison of the two datasets revealed very few homologies between sequences of apical organelle molecules, and provides evidence for sequence divergence between these closely-related parasites. The data presented underpin the potential value of the EST strategy for the discovery of novel genes and may allow for a more rapid increase in the knowledge and understanding of gene expression in the merozoite life cycle stage of
Eimeria spp. |
| doi_str_mv | 10.1016/S0020-7519(99)00160-5 |
| format | Article |
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Eimeria tenella. Of the ESTs, 47.7% had matches with entries in the databases, including ribosomal proteins, metabolic enzymes and proteins with other functions, of which 14.3% represented previously known
E. tenella genes. Thus over 50% of the ESTs had no significant database matches. The
E.
tenella EST dataset contained a range of highly abundant genes comparable with that found in the EST dataset of
T. gondii and may thus reflect the importance of such molecules in the biology of the apicomplexan organisms. However, comparison of the two datasets revealed very few homologies between sequences of apical organelle molecules, and provides evidence for sequence divergence between these closely-related parasites. The data presented underpin the potential value of the EST strategy for the discovery of novel genes and may allow for a more rapid increase in the knowledge and understanding of gene expression in the merozoite life cycle stage of
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Eimeria tenella. Of the ESTs, 47.7% had matches with entries in the databases, including ribosomal proteins, metabolic enzymes and proteins with other functions, of which 14.3% represented previously known
E. tenella genes. Thus over 50% of the ESTs had no significant database matches. The
E.
tenella EST dataset contained a range of highly abundant genes comparable with that found in the EST dataset of
T. gondii and may thus reflect the importance of such molecules in the biology of the apicomplexan organisms. However, comparison of the two datasets revealed very few homologies between sequences of apical organelle molecules, and provides evidence for sequence divergence between these closely-related parasites. The data presented underpin the potential value of the EST strategy for the discovery of novel genes and may allow for a more rapid increase in the knowledge and understanding of gene expression in the merozoite life cycle stage of
Eimeria spp.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Eimeria tenella</subject><subject>Eimeria tenella - genetics</subject><subject>Expressed sequence tagmerozoite</subject><subject>Expressed Sequence Tags</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Merozite</subject><subject>Molecular Sequence Data</subject><subject>Protozoa</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1LwzAUhoMobk5_gpILEb2oJm3SJFcyxvyAgReb1yFJkxHp2tm0g_nrTbept14cwjl5eM_HC8AlRvcY4fxhjlCKEkaxuBXiDsUSSugRGGLORIJwRo_B8BcZgLMQPiJEM0JOwQAjkWNOyBCMxzB0zcZuYe3g0lY2QF_BqV_ZxivYxkJZKhiz-qv2bfzVWzidL2Cwn52tjK-W5-DEqTLYi8M7Au9P08XkJZm9Pb9OxrPEZIy2iXaOYlVonedI8SJ3mhjMdYFpapAiGU-NczmznPMM85wQarQQTiucMRMjG4Gbve66qWPv0MqVD6Yfr7J1F2QuCGf0HyBmhCGGaASvDmCnV7aQ68avVLOVP9eJwPUBUMGo0jUqbhz-uDTOhXudxz1m4_obbxsZjI_HsYVvrGllUfuoKXvb5M422XsihZA72yTNvgFrq4c4</recordid><startdate>19991201</startdate><enddate>19991201</enddate><creator>Wan, Kiew-Lian</creator><creator>Chong, Saw-Peng</creator><creator>Ng, Szu-Ting</creator><creator>Shirley, Martin W</creator><creator>M.Tomley, Fiona</creator><creator>Sanusi Jangi, Mohd</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19991201</creationdate><title>A survey of genes in Eimeria tenella merozoites by EST sequencing</title><author>Wan, Kiew-Lian ; Chong, Saw-Peng ; Ng, Szu-Ting ; Shirley, Martin W ; M.Tomley, Fiona ; Sanusi Jangi, Mohd</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-bff51adbb660a8d6fb4c18bd152c0a4382cff67e8883186445cb99fba137c1373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Eimeria tenella</topic><topic>Eimeria tenella - genetics</topic><topic>Expressed sequence tagmerozoite</topic><topic>Expressed Sequence Tags</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Library</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Merozite</topic><topic>Molecular Sequence Data</topic><topic>Protozoa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wan, Kiew-Lian</creatorcontrib><creatorcontrib>Chong, Saw-Peng</creatorcontrib><creatorcontrib>Ng, Szu-Ting</creatorcontrib><creatorcontrib>Shirley, Martin W</creatorcontrib><creatorcontrib>M.Tomley, Fiona</creatorcontrib><creatorcontrib>Sanusi Jangi, Mohd</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wan, Kiew-Lian</au><au>Chong, Saw-Peng</au><au>Ng, Szu-Ting</au><au>Shirley, Martin W</au><au>M.Tomley, Fiona</au><au>Sanusi Jangi, Mohd</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A survey of genes in Eimeria tenella merozoites by EST sequencing</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>1999-12-01</date><risdate>1999</risdate><volume>29</volume><issue>12</issue><spage>1885</spage><epage>1892</epage><pages>1885-1892</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>A study of about 500 expressed sequence tags (ESTs), derived from a merozoite cDNA library, was initiated as an approach to generate a larger pool of gene information on
Eimeria tenella. Of the ESTs, 47.7% had matches with entries in the databases, including ribosomal proteins, metabolic enzymes and proteins with other functions, of which 14.3% represented previously known
E. tenella genes. Thus over 50% of the ESTs had no significant database matches. The
E.
tenella EST dataset contained a range of highly abundant genes comparable with that found in the EST dataset of
T. gondii and may thus reflect the importance of such molecules in the biology of the apicomplexan organisms. However, comparison of the two datasets revealed very few homologies between sequences of apical organelle molecules, and provides evidence for sequence divergence between these closely-related parasites. The data presented underpin the potential value of the EST strategy for the discovery of novel genes and may allow for a more rapid increase in the knowledge and understanding of gene expression in the merozoite life cycle stage of
Eimeria spp.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10961844</pmid><doi>10.1016/S0020-7519(99)00160-5</doi><tpages>8</tpages></addata></record> |
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| ispartof | International journal for parasitology, 1999-12, Vol.29 (12), p.1885-1892 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Eimeria tenella Eimeria tenella - genetics Expressed sequence tagmerozoite Expressed Sequence Tags Fundamental and applied biological sciences. Psychology Gene Library Life cycle. Host-agent relationship. Pathogenesis Merozite Molecular Sequence Data Protozoa |
| title | A survey of genes in Eimeria tenella merozoites by EST sequencing |
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