Influence of 16S rDNA primer sequence mismatches on the spectrum of bacterial genera detected in prostate tissue by universal eubacterial PCR

BACKGROUND Propionibacterium sp. and Staphylococcus spp. are the most frequent bacteria cultured from prostatectomy specimens but are seldom detected by universal eubacterial PCR. MATERIALS AND METHODS We obtained from GenBank representative 16S rRNA gene sequences from Propionibacterium sp., Staphy...

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Veröffentlicht in:	The Prostate 2008-10, Vol.68 (14), p.1487-1491
Hauptverfasser:	Shannon, Beverley A., Cohen, Ronald J., Garrett, Kerryn L.
Format:	Artikel
Sprache:	eng
Schlagworte:	bacterial detection bacterial infection benign prostatic hyperplasia DNA Primers - chemistry DNA Primers - genetics DNA, Bacterial - chemistry DNA, Bacterial - genetics Humans Male Polymerase Chain Reaction - methods Propionibacterium acnes - genetics Propionibacterium acnes - isolation & purification Prostate - microbiology prostate cancer prostatitis RNA, Ribosomal, 16S - chemistry RNA, Ribosomal, 16S - genetics Sequence Alignment Sequence Analysis, DNA Staphylococcus - genetics Staphylococcus - isolation & purification
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