P2Y2 receptor elevates intracellular calcium concentration in rabbit eye suprachoroid
Effects of ATP on the intracellular free calcium ion concentration ([Ca2+]i) in the rabbit eye suprachoroid were investigated by means of fura-2 microfluorophotometry. ATP application (10 to 100 microM) elicited a dose-dependent biphasic [Ca2+]i-increase: a fast phase typically peaking within 30 s a...
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| Veröffentlicht in: | Journal of medical and dental sciences 1999-06, Vol.46 (2), p.83-92 |
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| creator | Sugamoto, Y Hirai, K Tokoro, T |
| description | Effects of ATP on the intracellular free calcium ion concentration ([Ca2+]i) in the rabbit eye suprachoroid were investigated by means of fura-2 microfluorophotometry. ATP application (10 to 100 microM) elicited a dose-dependent biphasic [Ca2+]i-increase: a fast phase typically peaking within 30 s and a following slow plateau phase, which lasted during the presence of ATP. The slow plateau phase was markedly diminished by removal of extracellular Ca2+, whereas the fast phase remained. An inhibitor of Ca2+ release from the sarcoplasmic reticulum (TMB-8), an endoplasmic Ca2+-ATPase inhibitor (thapsigargin) and a phospholipase C (PLC) inhibitor (U-73122) diminished the fast phase. A P2 receptor antagonist (Suramin) inhibited the ATP-induced [Ca2+]i-response. The potency order of ATP and related substances in producing the [Ca2+]i-elevation was UTP approximately equals ATP>ATP-gamma-S>ITP>ADP. beta,gamma-MethyleneATP, 2-methylthioATP and UDP evoked no response. This order is consistent with the P2Y2 receptor characteristics. Cross-desensitization between ATP and UTP excludes the co-existence of the other types of receptors. In conclusion, the ATP-induced [Ca2+]i-elevation in the rabbit eye suprachoroid was elicited by the Ca2+ release from the PLC-dependent, thapsigargin-sensitive intracellular Ca2+ storage sites by activating P2Y2 nucleotide receptors. |
| doi_str_mv | 10.11480/jmds.460203 |
| format | Article |
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ATP application (10 to 100 microM) elicited a dose-dependent biphasic [Ca2+]i-increase: a fast phase typically peaking within 30 s and a following slow plateau phase, which lasted during the presence of ATP. The slow plateau phase was markedly diminished by removal of extracellular Ca2+, whereas the fast phase remained. An inhibitor of Ca2+ release from the sarcoplasmic reticulum (TMB-8), an endoplasmic Ca2+-ATPase inhibitor (thapsigargin) and a phospholipase C (PLC) inhibitor (U-73122) diminished the fast phase. A P2 receptor antagonist (Suramin) inhibited the ATP-induced [Ca2+]i-response. The potency order of ATP and related substances in producing the [Ca2+]i-elevation was UTP approximately equals ATP>ATP-gamma-S>ITP>ADP. beta,gamma-MethyleneATP, 2-methylthioATP and UDP evoked no response. This order is consistent with the P2Y2 receptor characteristics. Cross-desensitization between ATP and UTP excludes the co-existence of the other types of receptors. In conclusion, the ATP-induced [Ca2+]i-elevation in the rabbit eye suprachoroid was elicited by the Ca2+ release from the PLC-dependent, thapsigargin-sensitive intracellular Ca2+ storage sites by activating P2Y2 nucleotide receptors.</description><identifier>ISSN: 1342-8810</identifier><identifier>DOI: 10.11480/jmds.460203</identifier><identifier>PMID: 10805322</identifier><language>eng</language><publisher>Japan</publisher><subject>Adenosine Triphosphate - pharmacology ; Animals ; Calcium - metabolism ; Calcium Channel Blockers - pharmacology ; Calcium Signaling - drug effects ; Choroid - drug effects ; Choroid - metabolism ; Cytophotometry ; Dentistry ; Dose-Response Relationship, Drug ; Enzyme Inhibitors - pharmacology ; Estrenes - pharmacology ; Female ; Fluorescent Dyes ; Fura-2 ; Gallic Acid - analogs & derivatives ; Gallic Acid - pharmacology ; Male ; Phosphodiesterase Inhibitors - pharmacology ; Pyrrolidinones - pharmacology ; Rabbits ; Receptors, Purinergic P2 - drug effects ; Receptors, Purinergic P2 - physiology ; Receptors, Purinergic P2Y2 ; Sarcoplasmic Reticulum - drug effects ; Sarcoplasmic Reticulum - metabolism ; Statistics as Topic ; Suramin - pharmacology ; Thapsigargin - pharmacology ; Time Factors ; Type C Phospholipases - antagonists & inhibitors ; Uridine Triphosphate - pharmacology</subject><ispartof>Journal of medical and dental sciences, 1999-06, Vol.46 (2), p.83-92</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10805322$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugamoto, Y</creatorcontrib><creatorcontrib>Hirai, K</creatorcontrib><creatorcontrib>Tokoro, T</creatorcontrib><title>P2Y2 receptor elevates intracellular calcium concentration in rabbit eye suprachoroid</title><title>Journal of medical and dental sciences</title><addtitle>J Med Dent Sci</addtitle><description>Effects of ATP on the intracellular free calcium ion concentration ([Ca2+]i) in the rabbit eye suprachoroid were investigated by means of fura-2 microfluorophotometry. ATP application (10 to 100 microM) elicited a dose-dependent biphasic [Ca2+]i-increase: a fast phase typically peaking within 30 s and a following slow plateau phase, which lasted during the presence of ATP. The slow plateau phase was markedly diminished by removal of extracellular Ca2+, whereas the fast phase remained. An inhibitor of Ca2+ release from the sarcoplasmic reticulum (TMB-8), an endoplasmic Ca2+-ATPase inhibitor (thapsigargin) and a phospholipase C (PLC) inhibitor (U-73122) diminished the fast phase. A P2 receptor antagonist (Suramin) inhibited the ATP-induced [Ca2+]i-response. The potency order of ATP and related substances in producing the [Ca2+]i-elevation was UTP approximately equals ATP>ATP-gamma-S>ITP>ADP. beta,gamma-MethyleneATP, 2-methylthioATP and UDP evoked no response. This order is consistent with the P2Y2 receptor characteristics. Cross-desensitization between ATP and UTP excludes the co-existence of the other types of receptors. In conclusion, the ATP-induced [Ca2+]i-elevation in the rabbit eye suprachoroid was elicited by the Ca2+ release from the PLC-dependent, thapsigargin-sensitive intracellular Ca2+ storage sites by activating P2Y2 nucleotide receptors.</description><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Calcium Signaling - drug effects</subject><subject>Choroid - drug effects</subject><subject>Choroid - metabolism</subject><subject>Cytophotometry</subject><subject>Dentistry</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Estrenes - pharmacology</subject><subject>Female</subject><subject>Fluorescent Dyes</subject><subject>Fura-2</subject><subject>Gallic Acid - analogs & derivatives</subject><subject>Gallic Acid - pharmacology</subject><subject>Male</subject><subject>Phosphodiesterase Inhibitors - pharmacology</subject><subject>Pyrrolidinones - pharmacology</subject><subject>Rabbits</subject><subject>Receptors, Purinergic P2 - drug effects</subject><subject>Receptors, Purinergic P2 - physiology</subject><subject>Receptors, Purinergic P2Y2</subject><subject>Sarcoplasmic Reticulum - drug effects</subject><subject>Sarcoplasmic Reticulum - metabolism</subject><subject>Statistics as Topic</subject><subject>Suramin - pharmacology</subject><subject>Thapsigargin - pharmacology</subject><subject>Time Factors</subject><subject>Type C Phospholipases - antagonists & inhibitors</subject><subject>Uridine Triphosphate - pharmacology</subject><issn>1342-8810</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kD1PwzAYhD2AaClszMgTW4r9-qPOiCq-pEow0IEpsp3XIlUSBztB6r-nFWW64Z47nY6QG86WnEvD7nddnZdSM2DijMy5kFAYw9mMXOa8Y0yWRsoLMuPMMCUA5mT7Dp9AE3ocxpgotvhjR8y06cdkPbbt1NpEvW19M3XUx97j0Rmb2B8YmqxzzUhxjzRPwyHxFVNs6ityHmyb8fqkC7J9evxYvxSbt-fX9cOm2HEAKLSry1VpS2m5VwiCWWEkOhGYBR1QGQxasVJr0CrUTDEdwClZr7x2ngcQC3L31zuk-D1hHquuycfVtsc45UqXciVAlgfw9gROrsO6GlLT2bSv_o8Qv_pZXmo</recordid><startdate>199906</startdate><enddate>199906</enddate><creator>Sugamoto, Y</creator><creator>Hirai, K</creator><creator>Tokoro, T</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199906</creationdate><title>P2Y2 receptor elevates intracellular calcium concentration in rabbit eye suprachoroid</title><author>Sugamoto, Y ; Hirai, K ; Tokoro, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j1222-6bd979a94a1c5e230a384eb3f0a26fe58ef650966265fd0506f2b54d7c6bc1f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Calcium Signaling - drug effects</topic><topic>Choroid - drug effects</topic><topic>Choroid - metabolism</topic><topic>Cytophotometry</topic><topic>Dentistry</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Estrenes - pharmacology</topic><topic>Female</topic><topic>Fluorescent Dyes</topic><topic>Fura-2</topic><topic>Gallic Acid - analogs & derivatives</topic><topic>Gallic Acid - pharmacology</topic><topic>Male</topic><topic>Phosphodiesterase Inhibitors - pharmacology</topic><topic>Pyrrolidinones - pharmacology</topic><topic>Rabbits</topic><topic>Receptors, Purinergic P2 - drug effects</topic><topic>Receptors, Purinergic P2 - physiology</topic><topic>Receptors, Purinergic P2Y2</topic><topic>Sarcoplasmic Reticulum - drug effects</topic><topic>Sarcoplasmic Reticulum - metabolism</topic><topic>Statistics as Topic</topic><topic>Suramin - pharmacology</topic><topic>Thapsigargin - pharmacology</topic><topic>Time Factors</topic><topic>Type C Phospholipases - antagonists & inhibitors</topic><topic>Uridine Triphosphate - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugamoto, Y</creatorcontrib><creatorcontrib>Hirai, K</creatorcontrib><creatorcontrib>Tokoro, T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical and dental sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugamoto, Y</au><au>Hirai, K</au><au>Tokoro, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>P2Y2 receptor elevates intracellular calcium concentration in rabbit eye suprachoroid</atitle><jtitle>Journal of medical and dental sciences</jtitle><addtitle>J Med Dent Sci</addtitle><date>1999-06</date><risdate>1999</risdate><volume>46</volume><issue>2</issue><spage>83</spage><epage>92</epage><pages>83-92</pages><issn>1342-8810</issn><abstract>Effects of ATP on the intracellular free calcium ion concentration ([Ca2+]i) in the rabbit eye suprachoroid were investigated by means of fura-2 microfluorophotometry. ATP application (10 to 100 microM) elicited a dose-dependent biphasic [Ca2+]i-increase: a fast phase typically peaking within 30 s and a following slow plateau phase, which lasted during the presence of ATP. The slow plateau phase was markedly diminished by removal of extracellular Ca2+, whereas the fast phase remained. An inhibitor of Ca2+ release from the sarcoplasmic reticulum (TMB-8), an endoplasmic Ca2+-ATPase inhibitor (thapsigargin) and a phospholipase C (PLC) inhibitor (U-73122) diminished the fast phase. A P2 receptor antagonist (Suramin) inhibited the ATP-induced [Ca2+]i-response. The potency order of ATP and related substances in producing the [Ca2+]i-elevation was UTP approximately equals ATP>ATP-gamma-S>ITP>ADP. beta,gamma-MethyleneATP, 2-methylthioATP and UDP evoked no response. This order is consistent with the P2Y2 receptor characteristics. Cross-desensitization between ATP and UTP excludes the co-existence of the other types of receptors. In conclusion, the ATP-induced [Ca2+]i-elevation in the rabbit eye suprachoroid was elicited by the Ca2+ release from the PLC-dependent, thapsigargin-sensitive intracellular Ca2+ storage sites by activating P2Y2 nucleotide receptors.</abstract><cop>Japan</cop><pmid>10805322</pmid><doi>10.11480/jmds.460203</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE (Free - Japanese); MEDLINE; Alma/SFX Local Collection |
| subjects | Adenosine Triphosphate - pharmacology Animals Calcium - metabolism Calcium Channel Blockers - pharmacology Calcium Signaling - drug effects Choroid - drug effects Choroid - metabolism Cytophotometry Dentistry Dose-Response Relationship, Drug Enzyme Inhibitors - pharmacology Estrenes - pharmacology Female Fluorescent Dyes Fura-2 Gallic Acid - analogs & derivatives Gallic Acid - pharmacology Male Phosphodiesterase Inhibitors - pharmacology Pyrrolidinones - pharmacology Rabbits Receptors, Purinergic P2 - drug effects Receptors, Purinergic P2 - physiology Receptors, Purinergic P2Y2 Sarcoplasmic Reticulum - drug effects Sarcoplasmic Reticulum - metabolism Statistics as Topic Suramin - pharmacology Thapsigargin - pharmacology Time Factors Type C Phospholipases - antagonists & inhibitors Uridine Triphosphate - pharmacology |
| title | P2Y2 receptor elevates intracellular calcium concentration in rabbit eye suprachoroid |
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