Preparation and characterization of cross-linked enzyme aggregates (CLEA) of Subtilisin for controlled release applications

Enzyme stabilization is one of the major challenges in the biocatalytic process optimization. Subtilisin was aggregated using ammonium sulphate and polyethylene glycol with surfactants like triton X-100 and tween 20. The resultant aggregates on cross-linking with glutaraldehyde produced insoluble an...

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Veröffentlicht in:	International journal of biological macromolecules 2008-10, Vol.43 (3), p.314-319
Hauptverfasser:	Sangeetha, K., Emilia Abraham, T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Administration, Oral Aggregation Bacillus subtilis - enzymology Biotransformation CLEA Controlled protein release Cross-linking Cross-Linking Reagents - chemistry Delayed-Action Preparations Drug Carriers - chemistry Enzyme Stability Enzymes, Immobilized - metabolism Glutaral - chemistry Hydrogel beads Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry Hydrogen-Ion Concentration Kinetics Microscopy, Electron, Scanning Organic Chemicals - chemistry Organic solvents Protein Binding Solvents - chemistry Subtilisin Subtilisin - chemistry Subtilisin - metabolism Temperature Thermal stability
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