High-efficiency DNA injection into a single human mesenchymal stem cell using a nanoneedle and atomic force microscopy

Abstract We describe a low-invasive gene delivery method that uses an etched atomic force microscopy (AFM) tip or nanoneedle that can be inserted into a cell nucleus without causing cellular damage. The nanoneedle is 200 nm in diameter and 6 μm in length and is operated using an AFM system. The prob...

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Veröffentlicht in:	Nanomedicine 2008-09, Vol.4 (3), p.215-225
Hauptverfasser:	Han, Sung-Woong, PhD, Nakamura, Chikashi, PhD, Kotobuki, Noriko, PhD, Obataya, Ikuo, PhD, Ohgushi, Hajime, PhD, Nagamune, Teruyuki, PhD, Miyake, Jun, PhD
Format:	Artikel
Sprache:	eng
Schlagworte:	Atomic force microscopy (AFM) Cell Line Cell Nucleus Confocal microscopy DNA - administration & dosage Gene expression Gene transfer Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Humans Internal Medicine Mesenchymal stem cells Mesenchymal Stromal Cells Microinjections Microscopy, Atomic Force Nanoindentation Nanotechnology Plasmids Transfection - methods
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