Cellular responses to MPT-51, GlcB and ESAT-6 among MDR-TB and active tuberculosis patients in Brazil
Summary Multi-drug resistant pulmonary tuberculosis (MDR-TB) may result from either insufficiency of the host cellular immune response or mycobacterial mechanisms of resistance. Mycobacterium tuberculosis -specific CD8+ and CD4+ T lymphocytes from MDR-TB patients are poorly studied. The aim of this...
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| Veröffentlicht in: | Tuberculosis (Edinburgh, Scotland) Scotland), 2008-09, Vol.88 (5), p.474-481 |
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| creator | de Araújo-Filho, João Alves Vasconcelos, Arioldo Carvalho Martins de Sousa, Eduardo Kipnis, André Ribeiro, Elisângela Junqueira-Kipnis, Ana Paula |
| description | Summary Multi-drug resistant pulmonary tuberculosis (MDR-TB) may result from either insufficiency of the host cellular immune response or mycobacterial mechanisms of resistance. Mycobacterium tuberculosis -specific CD8+ and CD4+ T lymphocytes from MDR-TB patients are poorly studied. The aim of this study was to evaluate CD4+ IFN-γ+ , CD4+ IL-10+ , CD8+ IFN-γ+ and CD8+ IL-10+ cell populations by flow cytometry in non-resistant TB and multi-drug resistant tuberculosis (MDR-TB) patients from mid-central Brazil after stimulation with MPT-51, GlcB and ESAT-6 recombinant antigens from M. tuberculosis in comparison to tuberculin skin test negative (TST) healthy individuals. Non-resistant TB patients present specific cellular responses (CD4 and CD8, both IFN-γ and IL-10) to GlcB, MPT-51 and ESAT-6; while MDR-TB patients present only CD8+ IFN-γ+ responses to ESAT-6 and CD8+ IL-10+ responses to GlcB and ESAT-6. The results show that MDR-TB patients present impaired specific CD4 IFN-γ and IL-10 responses and increased/normal specific CD8 IFN-γ and IL-10 responses. This suggests an important role for CD8 function in these patients. |
| doi_str_mv | 10.1016/j.tube.2008.06.002 |
| format | Article |
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Mycobacterium tuberculosis -specific CD8+ and CD4+ T lymphocytes from MDR-TB patients are poorly studied. The aim of this study was to evaluate CD4+ IFN-γ+ , CD4+ IL-10+ , CD8+ IFN-γ+ and CD8+ IL-10+ cell populations by flow cytometry in non-resistant TB and multi-drug resistant tuberculosis (MDR-TB) patients from mid-central Brazil after stimulation with MPT-51, GlcB and ESAT-6 recombinant antigens from M. tuberculosis in comparison to tuberculin skin test negative (TST) healthy individuals. Non-resistant TB patients present specific cellular responses (CD4 and CD8, both IFN-γ and IL-10) to GlcB, MPT-51 and ESAT-6; while MDR-TB patients present only CD8+ IFN-γ+ responses to ESAT-6 and CD8+ IL-10+ responses to GlcB and ESAT-6. The results show that MDR-TB patients present impaired specific CD4 IFN-γ and IL-10 responses and increased/normal specific CD8 IFN-γ and IL-10 responses. This suggests an important role for CD8 function in these patients.</description><identifier>ISSN: 1472-9792</identifier><identifier>EISSN: 1873-281X</identifier><identifier>DOI: 10.1016/j.tube.2008.06.002</identifier><identifier>PMID: 18676203</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>Adult ; Aged ; Antigens, Bacterial - immunology ; Antigens, Bacterial - isolation & purification ; Bacterial Proteins - isolation & purification ; Brazil ; CD4-CD8 Ratio ; CD4-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - immunology ; Female ; GlcB ; Humans ; Infectious Disease ; Interferon-γ ; Interleukin-10 ; Interleukin-10 - immunology ; Male ; Middle Aged ; MPT-51 ; Multi-drug resistant tuberculosis ; Mycobacterium ; Mycobacterium tuberculosis - immunology ; Pulmonary/Respiratory ; Tuberculosis, Multidrug-Resistant - immunology ; Tuberculosis, Pulmonary - immunology</subject><ispartof>Tuberculosis (Edinburgh, Scotland), 2008-09, Vol.88 (5), p.474-481</ispartof><rights>Elsevier Ltd</rights><rights>2008 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-347d1525d343a092aa350ccab012d84acacf88215ecbd3b4d56e8f628864f08d3</citedby><cites>FETCH-LOGICAL-c440t-347d1525d343a092aa350ccab012d84acacf88215ecbd3b4d56e8f628864f08d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tube.2008.06.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18676203$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Araújo-Filho, João Alves</creatorcontrib><creatorcontrib>Vasconcelos, Arioldo Carvalho</creatorcontrib><creatorcontrib>Martins de Sousa, Eduardo</creatorcontrib><creatorcontrib>Kipnis, André</creatorcontrib><creatorcontrib>Ribeiro, Elisângela</creatorcontrib><creatorcontrib>Junqueira-Kipnis, Ana Paula</creatorcontrib><title>Cellular responses to MPT-51, GlcB and ESAT-6 among MDR-TB and active tuberculosis patients in Brazil</title><title>Tuberculosis (Edinburgh, Scotland)</title><addtitle>Tuberculosis (Edinb)</addtitle><description>Summary Multi-drug resistant pulmonary tuberculosis (MDR-TB) may result from either insufficiency of the host cellular immune response or mycobacterial mechanisms of resistance. Mycobacterium tuberculosis -specific CD8+ and CD4+ T lymphocytes from MDR-TB patients are poorly studied. The aim of this study was to evaluate CD4+ IFN-γ+ , CD4+ IL-10+ , CD8+ IFN-γ+ and CD8+ IL-10+ cell populations by flow cytometry in non-resistant TB and multi-drug resistant tuberculosis (MDR-TB) patients from mid-central Brazil after stimulation with MPT-51, GlcB and ESAT-6 recombinant antigens from M. tuberculosis in comparison to tuberculin skin test negative (TST) healthy individuals. Non-resistant TB patients present specific cellular responses (CD4 and CD8, both IFN-γ and IL-10) to GlcB, MPT-51 and ESAT-6; while MDR-TB patients present only CD8+ IFN-γ+ responses to ESAT-6 and CD8+ IL-10+ responses to GlcB and ESAT-6. The results show that MDR-TB patients present impaired specific CD4 IFN-γ and IL-10 responses and increased/normal specific CD8 IFN-γ and IL-10 responses. This suggests an important role for CD8 function in these patients.</description><subject>Adult</subject><subject>Aged</subject><subject>Antigens, Bacterial - immunology</subject><subject>Antigens, Bacterial - isolation & purification</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Brazil</subject><subject>CD4-CD8 Ratio</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Female</subject><subject>GlcB</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Interferon-γ</subject><subject>Interleukin-10</subject><subject>Interleukin-10 - immunology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>MPT-51</subject><subject>Multi-drug resistant tuberculosis</subject><subject>Mycobacterium</subject><subject>Mycobacterium tuberculosis - immunology</subject><subject>Pulmonary/Respiratory</subject><subject>Tuberculosis, Multidrug-Resistant - immunology</subject><subject>Tuberculosis, Pulmonary - immunology</subject><issn>1472-9792</issn><issn>1873-281X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkl9rFDEUxYMotla_gA-SJ5-c8SaTSbIgQrv2H7QodoW-hUxyR7LOzmyTmUL99M2wCwUf7FNC-J1D7jmXkPcMSgZMfl6X49RgyQF0CbIE4C_IIdOqKrhmty_zXSheLNSCH5A3Ka0hi0DDa3LAtFSSQ3VIcIldN3U20ohpO_QJEx0Hev1jVdTsEz3v3Am1vaenN8erQlK7Gfrf9Prbz2K1e7duDPdI549EN3VDColu7RiwHxMNPT2J9m_o3pJXre0SvtufR-TX2elqeVFcfT-_XB5fFU4IGItKKM9qXvtKVBYW3NqqBudsA4x7LayzrtWasxpd46tG-FqibiXXWooWtK-OyMed7zYOdxOm0WxCcnlC2-MwJSMXolZM1c-CnIEQUrEM8h3o4pBSxNZsY9jY-GAYmLkFszbz8GZuwYA0uYUs-rB3n5oN-ifJPvYMfNkBmMO4DxhNcjkyhz5EdKPxQ_i__9d_5K4LfXC2-4MPmNbDFPscs2EmcQPmZt6DeQ1y-QBS3VaPPwSrjg</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>de Araújo-Filho, João Alves</creator><creator>Vasconcelos, Arioldo Carvalho</creator><creator>Martins de Sousa, Eduardo</creator><creator>Kipnis, André</creator><creator>Ribeiro, Elisângela</creator><creator>Junqueira-Kipnis, Ana Paula</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>Cellular responses to MPT-51, GlcB and ESAT-6 among MDR-TB and active tuberculosis patients in Brazil</title><author>de Araújo-Filho, João Alves ; Vasconcelos, Arioldo Carvalho ; Martins de Sousa, Eduardo ; Kipnis, André ; Ribeiro, Elisângela ; Junqueira-Kipnis, Ana Paula</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-347d1525d343a092aa350ccab012d84acacf88215ecbd3b4d56e8f628864f08d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Antigens, Bacterial - immunology</topic><topic>Antigens, Bacterial - isolation & purification</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Brazil</topic><topic>CD4-CD8 Ratio</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Female</topic><topic>GlcB</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Interferon-γ</topic><topic>Interleukin-10</topic><topic>Interleukin-10 - immunology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>MPT-51</topic><topic>Multi-drug resistant tuberculosis</topic><topic>Mycobacterium</topic><topic>Mycobacterium tuberculosis - immunology</topic><topic>Pulmonary/Respiratory</topic><topic>Tuberculosis, Multidrug-Resistant - immunology</topic><topic>Tuberculosis, Pulmonary - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Araújo-Filho, João Alves</creatorcontrib><creatorcontrib>Vasconcelos, Arioldo Carvalho</creatorcontrib><creatorcontrib>Martins de Sousa, Eduardo</creatorcontrib><creatorcontrib>Kipnis, André</creatorcontrib><creatorcontrib>Ribeiro, Elisângela</creatorcontrib><creatorcontrib>Junqueira-Kipnis, Ana Paula</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Tuberculosis (Edinburgh, Scotland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Araújo-Filho, João Alves</au><au>Vasconcelos, Arioldo Carvalho</au><au>Martins de Sousa, Eduardo</au><au>Kipnis, André</au><au>Ribeiro, Elisângela</au><au>Junqueira-Kipnis, Ana Paula</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular responses to MPT-51, GlcB and ESAT-6 among MDR-TB and active tuberculosis patients in Brazil</atitle><jtitle>Tuberculosis (Edinburgh, Scotland)</jtitle><addtitle>Tuberculosis (Edinb)</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>88</volume><issue>5</issue><spage>474</spage><epage>481</epage><pages>474-481</pages><issn>1472-9792</issn><eissn>1873-281X</eissn><abstract>Summary Multi-drug resistant pulmonary tuberculosis (MDR-TB) may result from either insufficiency of the host cellular immune response or mycobacterial mechanisms of resistance. Mycobacterium tuberculosis -specific CD8+ and CD4+ T lymphocytes from MDR-TB patients are poorly studied. The aim of this study was to evaluate CD4+ IFN-γ+ , CD4+ IL-10+ , CD8+ IFN-γ+ and CD8+ IL-10+ cell populations by flow cytometry in non-resistant TB and multi-drug resistant tuberculosis (MDR-TB) patients from mid-central Brazil after stimulation with MPT-51, GlcB and ESAT-6 recombinant antigens from M. tuberculosis in comparison to tuberculin skin test negative (TST) healthy individuals. Non-resistant TB patients present specific cellular responses (CD4 and CD8, both IFN-γ and IL-10) to GlcB, MPT-51 and ESAT-6; while MDR-TB patients present only CD8+ IFN-γ+ responses to ESAT-6 and CD8+ IL-10+ responses to GlcB and ESAT-6. The results show that MDR-TB patients present impaired specific CD4 IFN-γ and IL-10 responses and increased/normal specific CD8 IFN-γ and IL-10 responses. This suggests an important role for CD8 function in these patients.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>18676203</pmid><doi>10.1016/j.tube.2008.06.002</doi><tpages>8</tpages></addata></record> |
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| ispartof | Tuberculosis (Edinburgh, Scotland), 2008-09, Vol.88 (5), p.474-481 |
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| subjects | Adult Aged Antigens, Bacterial - immunology Antigens, Bacterial - isolation & purification Bacterial Proteins - isolation & purification Brazil CD4-CD8 Ratio CD4-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - immunology Female GlcB Humans Infectious Disease Interferon-γ Interleukin-10 Interleukin-10 - immunology Male Middle Aged MPT-51 Multi-drug resistant tuberculosis Mycobacterium Mycobacterium tuberculosis - immunology Pulmonary/Respiratory Tuberculosis, Multidrug-Resistant - immunology Tuberculosis, Pulmonary - immunology |
| title | Cellular responses to MPT-51, GlcB and ESAT-6 among MDR-TB and active tuberculosis patients in Brazil |
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