Differentiations of transplanted mouse spermatogonial stem cells in the adult mouse renal parenchyma in vivo
Aim： Spermatogonial stem cells can initiate the process of cellular differentiation to generate mature spermatozoa, but whether it possess the characteristic of pluripotency and plasticity, similar to embryonic stem cells, has not been elucidated. This study was designed to evaluate the differentiat...
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| Veröffentlicht in: | Acta pharmacologica Sinica 2008-09, Vol.29 (9), p.1029-1034 |
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| creator | Wu, Da-peng He, Da-lin Li, Xiang Liu, Zhao-hui |
| description | Aim： Spermatogonial stem cells can initiate the process of cellular differentiation to generate mature spermatozoa, but whether it possess the characteristic of pluripotency and plasticity, similar to embryonic stem cells, has not been elucidated. This study was designed to evaluate the differentiation potential of spermatogonial stem cells into renal cells in vivo. Methods： Neonatal mouse spermatogonial stem cells were transplanted into mature male mice lacking endogenous spermatogenesis. The restoration of fertility in recipient males was observed. Spermatogonial stem cells were then injected into renal parenchyma of mature female mice to make a new extracellular environment for differentiation. Fluorescence in situ hybridization technology （FISH） was used to detect the expression of chromosome Y in recipient renal tissues. To determine the type of cells differentiated from spermatogonial stem cells, the expression of ricinus communis agglutinin, vimentin, CD45, and F4/80 proteins were examined in the renal tissues by immunohistochemistry. Results： The proliferation of seminiferous epithelial cells was distinctly observed in seminiferous tubules of transplanted testes, whereas no regeneration of spermatogenesis was observed in non-transplanted control testes. In transplanted female renal tissues, FISH showed a much stronger immuno-fluorescence signal of chromosome Y in the nucleolus of epithelial cells of the renal tubule and podocytes of the glomerulus. Conclusion： The spermatogonial stem cells were successfully purified from mouse testicles. This finding demonstrated that spermatogonial stem cells could not only restore damaged spermatogenesis, but were also capable of differentiating into mature renal parenchyma cells in vivo. |
| doi_str_mv | 10.1111/j.1745-7254.2008.00836.x |
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This study was designed to evaluate the differentiation potential of spermatogonial stem cells into renal cells in vivo. Methods： Neonatal mouse spermatogonial stem cells were transplanted into mature male mice lacking endogenous spermatogenesis. The restoration of fertility in recipient males was observed. Spermatogonial stem cells were then injected into renal parenchyma of mature female mice to make a new extracellular environment for differentiation. Fluorescence in situ hybridization technology （FISH） was used to detect the expression of chromosome Y in recipient renal tissues. To determine the type of cells differentiated from spermatogonial stem cells, the expression of ricinus communis agglutinin, vimentin, CD45, and F4/80 proteins were examined in the renal tissues by immunohistochemistry. Results： The proliferation of seminiferous epithelial cells was distinctly observed in seminiferous tubules of transplanted testes, whereas no regeneration of spermatogenesis was observed in non-transplanted control testes. In transplanted female renal tissues, FISH showed a much stronger immuno-fluorescence signal of chromosome Y in the nucleolus of epithelial cells of the renal tubule and podocytes of the glomerulus. Conclusion： The spermatogonial stem cells were successfully purified from mouse testicles. This finding demonstrated that spermatogonial stem cells could not only restore damaged spermatogenesis, but were also capable of differentiating into mature renal parenchyma cells in vivo.</description><identifier>ISSN: 1671-4083</identifier><identifier>EISSN: 1745-7254</identifier><identifier>DOI: 10.1111/j.1745-7254.2008.00836.x</identifier><identifier>PMID: 18718171</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Animals, Newborn ; Biomedical and Life Sciences ; Biomedicine ; Cell Differentiation - physiology ; Gene Expression Regulation ; Immunology ; Internal Medicine ; Kidney - cytology ; Male ; Medical Microbiology ; Mice ; Mice, Inbred BALB C ; original-article ; Pharmacology/Toxicology ; Pluripotent Stem Cells - physiology ; Ricinus communis ; Spermatogonia - physiology ; Spermatogonia - transplantation ; Stem Cell Transplantation ; Testis - cytology ; Vaccine ; X Chromosome - genetics ; 干细胞 ; 生精细胞 ; 精子产生</subject><ispartof>Acta pharmacologica Sinica, 2008-09, Vol.29 (9), p.1029-1034</ispartof><rights>CPS and SIMM 2008</rights><rights>Copyright Nature Publishing Group Sep 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c344t-bca397930c43a3ed77d59a8b49b2d10e0e958428390b8c5731c38effa39b50993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/95561A/95561A.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18718171$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Da-peng</creatorcontrib><creatorcontrib>He, Da-lin</creatorcontrib><creatorcontrib>Li, Xiang</creatorcontrib><creatorcontrib>Liu, Zhao-hui</creatorcontrib><title>Differentiations of transplanted mouse spermatogonial stem cells in the adult mouse renal parenchyma in vivo</title><title>Acta pharmacologica Sinica</title><addtitle>Acta Pharmacol Sin</addtitle><addtitle>Acta Pharmacologica Sinica</addtitle><description>Aim： Spermatogonial stem cells can initiate the process of cellular differentiation to generate mature spermatozoa, but whether it possess the characteristic of pluripotency and plasticity, similar to embryonic stem cells, has not been elucidated. This study was designed to evaluate the differentiation potential of spermatogonial stem cells into renal cells in vivo. Methods： Neonatal mouse spermatogonial stem cells were transplanted into mature male mice lacking endogenous spermatogenesis. The restoration of fertility in recipient males was observed. Spermatogonial stem cells were then injected into renal parenchyma of mature female mice to make a new extracellular environment for differentiation. Fluorescence in situ hybridization technology （FISH） was used to detect the expression of chromosome Y in recipient renal tissues. To determine the type of cells differentiated from spermatogonial stem cells, the expression of ricinus communis agglutinin, vimentin, CD45, and F4/80 proteins were examined in the renal tissues by immunohistochemistry. Results： The proliferation of seminiferous epithelial cells was distinctly observed in seminiferous tubules of transplanted testes, whereas no regeneration of spermatogenesis was observed in non-transplanted control testes. In transplanted female renal tissues, FISH showed a much stronger immuno-fluorescence signal of chromosome Y in the nucleolus of epithelial cells of the renal tubule and podocytes of the glomerulus. Conclusion： The spermatogonial stem cells were successfully purified from mouse testicles. This finding demonstrated that spermatogonial stem cells could not only restore damaged spermatogenesis, but were also capable of differentiating into mature renal parenchyma cells in vivo.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Differentiation - physiology</subject><subject>Gene Expression Regulation</subject><subject>Immunology</subject><subject>Internal Medicine</subject><subject>Kidney - cytology</subject><subject>Male</subject><subject>Medical Microbiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>original-article</subject><subject>Pharmacology/Toxicology</subject><subject>Pluripotent Stem Cells - physiology</subject><subject>Ricinus communis</subject><subject>Spermatogonia - physiology</subject><subject>Spermatogonia - transplantation</subject><subject>Stem Cell Transplantation</subject><subject>Testis - cytology</subject><subject>Vaccine</subject><subject>X Chromosome - genetics</subject><subject>干细胞</subject><subject>生精细胞</subject><subject>精子产生</subject><issn>1671-4083</issn><issn>1745-7254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkc1u1TAQhS0EoqXlFZDFgl2Cf2N7iQotSJW6adeW4zj3-pLYaexU7dtj0wuV2GDJGkvzzZnxHAAgRi0u5_OhxYLxRhDOWoKQbMulXfv4Cpz-Tbwu707ghpXUCXiX0gEhSihWb8EJlgJLLPApmL76cXSrC9mb7GNIMI4wryakZTIhuwHOcUsOpsWts8lxF4M3E0zZzdC6aUrQB5j3Dpphm_IRLnKFWUyJdv80m8o8-Id4Dt6MZkru_TGegbvLb7cX35vrm6sfF1-uG0sZy01vDVVCUWQZNdQNQgxcGdkz1ZMBI4ec4pIRSRXqpeWCYkulG8dS1XOkFD0Dn551lzXeby5lPftUpzXBlQF1pxjndS__AwlGWImugh__AQ9xW8svK0MR4ZjVth-O0NbPbtDL6mezPuk_2y6AegZSSYWdW19UMNLVWX3Q1UBdDdTVWf3bWf34MoHdx7C7L-W6N_bn6CeniWQdl4TSX8VUoQE</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Wu, Da-peng</creator><creator>He, Da-lin</creator><creator>Li, Xiang</creator><creator>Liu, Zhao-hui</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>Differentiations of transplanted mouse spermatogonial stem cells in the adult mouse renal parenchyma in vivo</title><author>Wu, Da-peng ; He, Da-lin ; Li, Xiang ; Liu, Zhao-hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-bca397930c43a3ed77d59a8b49b2d10e0e958428390b8c5731c38effa39b50993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Differentiation - physiology</topic><topic>Gene Expression Regulation</topic><topic>Immunology</topic><topic>Internal Medicine</topic><topic>Kidney - cytology</topic><topic>Male</topic><topic>Medical Microbiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>original-article</topic><topic>Pharmacology/Toxicology</topic><topic>Pluripotent Stem Cells - physiology</topic><topic>Ricinus communis</topic><topic>Spermatogonia - physiology</topic><topic>Spermatogonia - transplantation</topic><topic>Stem Cell Transplantation</topic><topic>Testis - cytology</topic><topic>Vaccine</topic><topic>X Chromosome - genetics</topic><topic>干细胞</topic><topic>生精细胞</topic><topic>精子产生</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Da-peng</creatorcontrib><creatorcontrib>He, Da-lin</creatorcontrib><creatorcontrib>Li, Xiang</creatorcontrib><creatorcontrib>Liu, Zhao-hui</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta pharmacologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Da-peng</au><au>He, Da-lin</au><au>Li, Xiang</au><au>Liu, Zhao-hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentiations of transplanted mouse spermatogonial stem cells in the adult mouse renal parenchyma in vivo</atitle><jtitle>Acta pharmacologica Sinica</jtitle><stitle>Acta Pharmacol Sin</stitle><addtitle>Acta Pharmacologica Sinica</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>29</volume><issue>9</issue><spage>1029</spage><epage>1034</epage><pages>1029-1034</pages><issn>1671-4083</issn><eissn>1745-7254</eissn><abstract>Aim： Spermatogonial stem cells can initiate the process of cellular differentiation to generate mature spermatozoa, but whether it possess the characteristic of pluripotency and plasticity, similar to embryonic stem cells, has not been elucidated. This study was designed to evaluate the differentiation potential of spermatogonial stem cells into renal cells in vivo. Methods： Neonatal mouse spermatogonial stem cells were transplanted into mature male mice lacking endogenous spermatogenesis. The restoration of fertility in recipient males was observed. Spermatogonial stem cells were then injected into renal parenchyma of mature female mice to make a new extracellular environment for differentiation. Fluorescence in situ hybridization technology （FISH） was used to detect the expression of chromosome Y in recipient renal tissues. To determine the type of cells differentiated from spermatogonial stem cells, the expression of ricinus communis agglutinin, vimentin, CD45, and F4/80 proteins were examined in the renal tissues by immunohistochemistry. Results： The proliferation of seminiferous epithelial cells was distinctly observed in seminiferous tubules of transplanted testes, whereas no regeneration of spermatogenesis was observed in non-transplanted control testes. In transplanted female renal tissues, FISH showed a much stronger immuno-fluorescence signal of chromosome Y in the nucleolus of epithelial cells of the renal tubule and podocytes of the glomerulus. Conclusion： The spermatogonial stem cells were successfully purified from mouse testicles. This finding demonstrated that spermatogonial stem cells could not only restore damaged spermatogenesis, but were also capable of differentiating into mature renal parenchyma cells in vivo.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>18718171</pmid><doi>10.1111/j.1745-7254.2008.00836.x</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Animals, Newborn Biomedical and Life Sciences Biomedicine Cell Differentiation - physiology Gene Expression Regulation Immunology Internal Medicine Kidney - cytology Male Medical Microbiology Mice Mice, Inbred BALB C original-article Pharmacology/Toxicology Pluripotent Stem Cells - physiology Ricinus communis Spermatogonia - physiology Spermatogonia - transplantation Stem Cell Transplantation Testis - cytology Vaccine X Chromosome - genetics 干细胞 生精细胞 精子产生 |
| title | Differentiations of transplanted mouse spermatogonial stem cells in the adult mouse renal parenchyma in vivo |
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