Differential presentation of tumor antigen‐derived epitopes by MHC‐class I and antigen‐positive tumor cells
SSX2 is a member of the family of cancer/testis antigens. The SSX2 derived peptide SSX2103–111 has been shown to be presented to cytotoxic T‐lymphocytes (CTL) by Major‐Histocompatibility (MHC) Class‐I complexes after endogenous processing, more precisely by the allele HLA‐A*0201. The HLA‐A*0201‐ and...
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| Veröffentlicht in: | International journal of cancer 2008-10, Vol.123 (8), p.1841-1847 |
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| creator | Held, Gerhard Neumann, Frank Sturm, Christine Kaestner, Lars Dauth, Nina de Bruijn, Diederik, R. Renner, Christoph Lipp, Peter Pfreundschuh, Michael |
| description | SSX2 is a member of the family of cancer/testis antigens. The SSX2 derived peptide SSX2103–111 has been shown to be presented to cytotoxic T‐lymphocytes (CTL) by Major‐Histocompatibility (MHC) Class‐I complexes after endogenous processing, more precisely by the allele HLA‐A*0201. The HLA‐A*0201‐ and SSX2‐positive melanoma cell line SK‐Mel‐37 but not Me275 had been shown to elicit reactivity in SSX2103–111 specific cytotoxic T‐lymphocytes. To analyze the correlation between SSX2103–111 presentation and T‐cell stimulation, we intended to visualize presentation of SSX2103–111 in these melanoma cell lines. Fab‐antibodies were established from a human phage library with specificity for SSX2103–111/HLA‐A*0201 complexes (but non‐reactive with HLA‐A*0201 or SSX2103–111 alone) and used to visualize the presentation of SSX2103–111 in the context of HLA‐A*0201 by fluorescence microscopy. Presentation of SSX2103–111 the context of HLA‐A*0201 was demonstrated for the majority of SK‐Mel‐37, but for only a small fraction ( |
| doi_str_mv | 10.1002/ijc.23728 |
| format | Article |
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The SSX2 derived peptide SSX2103–111 has been shown to be presented to cytotoxic T‐lymphocytes (CTL) by Major‐Histocompatibility (MHC) Class‐I complexes after endogenous processing, more precisely by the allele HLA‐A*0201. The HLA‐A*0201‐ and SSX2‐positive melanoma cell line SK‐Mel‐37 but not Me275 had been shown to elicit reactivity in SSX2103–111 specific cytotoxic T‐lymphocytes. To analyze the correlation between SSX2103–111 presentation and T‐cell stimulation, we intended to visualize presentation of SSX2103–111 in these melanoma cell lines. Fab‐antibodies were established from a human phage library with specificity for SSX2103–111/HLA‐A*0201 complexes (but non‐reactive with HLA‐A*0201 or SSX2103–111 alone) and used to visualize the presentation of SSX2103–111 in the context of HLA‐A*0201 by fluorescence microscopy. Presentation of SSX2103–111 the context of HLA‐A*0201 was demonstrated for the majority of SK‐Mel‐37, but for only a small fraction (<1%) of Me275 as indicated by a clear membrane‐staining pattern in fluorescence microscopy. The presentation of SSX2103–111 on SK‐Mel37 and Me275, but not the expression of the SSX2 protein correlated with the capability of these cells to stimulate cells of an SSX2103–111‐specific T‐cell clone. MHC‐peptide specific antibodies are a valuable tool for the analysis of antigenic peptides in the context of MHC‐I molecules and for the structural definition of immunodominant epitopes. © 2008 Wiley‐Liss, Inc.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.23728</identifier><identifier>PMID: 18688854</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>3T3 Cells ; Animals ; Antibody Specificity ; Antigen Presentation ; Antigens, Neoplasm - genetics ; Antigens, Neoplasm - immunology ; Biological and medical sciences ; Breast Neoplasms - genetics ; Breast Neoplasms - immunology ; Cell Line, Tumor ; HLA-A Antigens - immunology ; HLA-A2 Antigen ; Humans ; Immunoglobulin Fragments - immunology ; Medical sciences ; Melanoma - genetics ; Melanoma - immunology ; MHC‐peptide‐specific antibodies scope ; Mice ; Microscopy, Fluorescence ; Multiple tumors. Solid tumors. Tumors in childhood (general aspects) ; Neoplasm Proteins - biosynthesis ; Neoplasm Proteins - genetics ; Neoplasm Proteins - immunology ; Peptide Fragments - immunology ; Polymerase Chain Reaction ; Repressor Proteins - biosynthesis ; Repressor Proteins - genetics ; Repressor Proteins - immunology ; SSX2 ; T-Lymphocytes, Cytotoxic - immunology ; tumor immunology ; Tumors ; tumor‐antigen</subject><ispartof>International journal of cancer, 2008-10, Vol.123 (8), p.1841-1847</ispartof><rights>Copyright © 2008 Wiley‐Liss, Inc.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4198-aca9be9dafcec8298ae75179832fd74084badad16b8efd8762b57608049fd1973</citedby><cites>FETCH-LOGICAL-c4198-aca9be9dafcec8298ae75179832fd74084badad16b8efd8762b57608049fd1973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.23728$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.23728$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20618554$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18688854$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Held, Gerhard</creatorcontrib><creatorcontrib>Neumann, Frank</creatorcontrib><creatorcontrib>Sturm, Christine</creatorcontrib><creatorcontrib>Kaestner, Lars</creatorcontrib><creatorcontrib>Dauth, Nina</creatorcontrib><creatorcontrib>de Bruijn, Diederik, R.</creatorcontrib><creatorcontrib>Renner, Christoph</creatorcontrib><creatorcontrib>Lipp, Peter</creatorcontrib><creatorcontrib>Pfreundschuh, Michael</creatorcontrib><title>Differential presentation of tumor antigen‐derived epitopes by MHC‐class I and antigen‐positive tumor cells</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>SSX2 is a member of the family of cancer/testis antigens. The SSX2 derived peptide SSX2103–111 has been shown to be presented to cytotoxic T‐lymphocytes (CTL) by Major‐Histocompatibility (MHC) Class‐I complexes after endogenous processing, more precisely by the allele HLA‐A*0201. The HLA‐A*0201‐ and SSX2‐positive melanoma cell line SK‐Mel‐37 but not Me275 had been shown to elicit reactivity in SSX2103–111 specific cytotoxic T‐lymphocytes. To analyze the correlation between SSX2103–111 presentation and T‐cell stimulation, we intended to visualize presentation of SSX2103–111 in these melanoma cell lines. Fab‐antibodies were established from a human phage library with specificity for SSX2103–111/HLA‐A*0201 complexes (but non‐reactive with HLA‐A*0201 or SSX2103–111 alone) and used to visualize the presentation of SSX2103–111 in the context of HLA‐A*0201 by fluorescence microscopy. Presentation of SSX2103–111 the context of HLA‐A*0201 was demonstrated for the majority of SK‐Mel‐37, but for only a small fraction (<1%) of Me275 as indicated by a clear membrane‐staining pattern in fluorescence microscopy. The presentation of SSX2103–111 on SK‐Mel37 and Me275, but not the expression of the SSX2 protein correlated with the capability of these cells to stimulate cells of an SSX2103–111‐specific T‐cell clone. MHC‐peptide specific antibodies are a valuable tool for the analysis of antigenic peptides in the context of MHC‐I molecules and for the structural definition of immunodominant epitopes. © 2008 Wiley‐Liss, Inc.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Antigen Presentation</subject><subject>Antigens, Neoplasm - genetics</subject><subject>Antigens, Neoplasm - immunology</subject><subject>Biological and medical sciences</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - immunology</subject><subject>Cell Line, Tumor</subject><subject>HLA-A Antigens - immunology</subject><subject>HLA-A2 Antigen</subject><subject>Humans</subject><subject>Immunoglobulin Fragments - immunology</subject><subject>Medical sciences</subject><subject>Melanoma - genetics</subject><subject>Melanoma - immunology</subject><subject>MHC‐peptide‐specific antibodies scope</subject><subject>Mice</subject><subject>Microscopy, Fluorescence</subject><subject>Multiple tumors. Solid tumors. Tumors in childhood (general aspects)</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Proteins - immunology</subject><subject>Peptide Fragments - immunology</subject><subject>Polymerase Chain Reaction</subject><subject>Repressor Proteins - biosynthesis</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - immunology</subject><subject>SSX2</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>tumor immunology</subject><subject>Tumors</subject><subject>tumor‐antigen</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0b1OwzAQB3ALgWgpDLwAygISQ6jtfNgeUfkqKmKBOXLsMzJKk9ROQd14BJ6RJ8G0EbAgJlu6n-9O_iN0SPAZwZiO7bM6owmjfAsNCRYsxpRk22gYajhmJMkHaM_7Z4wJyXC6iwaE55zzLB2ixYU1BhzUnZVV1Drw4So729RRY6JuOW9cJEPxCeqPt3cNzr6AjqC1XdOCj8pVdHczCRVVSe-jabD6l28bb7vwom-koKr8PtoxsvJw0J8j9Hh1-TC5iWf319PJ-SxWKRE8lkqKEoSWRoHiVHAJLCNM8IQazVLM01JqqUlecjCas5yWGcsxx6kwmgiWjNDJpm_rmsUSfFfMrf_aQNbQLH2RizRLacL_hRTnNOO5CPB0A5VrvHdgitbZuXSrguDiK4giBFGsgwj2qG-6LOegf2T_8wEc90B6JSvjZK2s_3ZhKOHZ2o037tVWsPp7YjG9nWxGfwIBpKOw</recordid><startdate>20081015</startdate><enddate>20081015</enddate><creator>Held, Gerhard</creator><creator>Neumann, Frank</creator><creator>Sturm, Christine</creator><creator>Kaestner, Lars</creator><creator>Dauth, Nina</creator><creator>de Bruijn, Diederik, R.</creator><creator>Renner, Christoph</creator><creator>Lipp, Peter</creator><creator>Pfreundschuh, Michael</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20081015</creationdate><title>Differential presentation of tumor antigen‐derived epitopes by MHC‐class I and antigen‐positive tumor cells</title><author>Held, Gerhard ; Neumann, Frank ; Sturm, Christine ; Kaestner, Lars ; Dauth, Nina ; de Bruijn, Diederik, R. ; Renner, Christoph ; Lipp, Peter ; Pfreundschuh, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4198-aca9be9dafcec8298ae75179832fd74084badad16b8efd8762b57608049fd1973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>3T3 Cells</topic><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Antigen Presentation</topic><topic>Antigens, Neoplasm - genetics</topic><topic>Antigens, Neoplasm - immunology</topic><topic>Biological and medical sciences</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - immunology</topic><topic>Cell Line, Tumor</topic><topic>HLA-A Antigens - immunology</topic><topic>HLA-A2 Antigen</topic><topic>Humans</topic><topic>Immunoglobulin Fragments - immunology</topic><topic>Medical sciences</topic><topic>Melanoma - genetics</topic><topic>Melanoma - immunology</topic><topic>MHC‐peptide‐specific antibodies scope</topic><topic>Mice</topic><topic>Microscopy, Fluorescence</topic><topic>Multiple tumors. Solid tumors. Tumors in childhood (general aspects)</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Proteins - immunology</topic><topic>Peptide Fragments - immunology</topic><topic>Polymerase Chain Reaction</topic><topic>Repressor Proteins - biosynthesis</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - immunology</topic><topic>SSX2</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>tumor immunology</topic><topic>Tumors</topic><topic>tumor‐antigen</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Held, Gerhard</creatorcontrib><creatorcontrib>Neumann, Frank</creatorcontrib><creatorcontrib>Sturm, Christine</creatorcontrib><creatorcontrib>Kaestner, Lars</creatorcontrib><creatorcontrib>Dauth, Nina</creatorcontrib><creatorcontrib>de Bruijn, Diederik, R.</creatorcontrib><creatorcontrib>Renner, Christoph</creatorcontrib><creatorcontrib>Lipp, Peter</creatorcontrib><creatorcontrib>Pfreundschuh, Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Held, Gerhard</au><au>Neumann, Frank</au><au>Sturm, Christine</au><au>Kaestner, Lars</au><au>Dauth, Nina</au><au>de Bruijn, Diederik, R.</au><au>Renner, Christoph</au><au>Lipp, Peter</au><au>Pfreundschuh, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential presentation of tumor antigen‐derived epitopes by MHC‐class I and antigen‐positive tumor cells</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>2008-10-15</date><risdate>2008</risdate><volume>123</volume><issue>8</issue><spage>1841</spage><epage>1847</epage><pages>1841-1847</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>SSX2 is a member of the family of cancer/testis antigens. The SSX2 derived peptide SSX2103–111 has been shown to be presented to cytotoxic T‐lymphocytes (CTL) by Major‐Histocompatibility (MHC) Class‐I complexes after endogenous processing, more precisely by the allele HLA‐A*0201. The HLA‐A*0201‐ and SSX2‐positive melanoma cell line SK‐Mel‐37 but not Me275 had been shown to elicit reactivity in SSX2103–111 specific cytotoxic T‐lymphocytes. To analyze the correlation between SSX2103–111 presentation and T‐cell stimulation, we intended to visualize presentation of SSX2103–111 in these melanoma cell lines. Fab‐antibodies were established from a human phage library with specificity for SSX2103–111/HLA‐A*0201 complexes (but non‐reactive with HLA‐A*0201 or SSX2103–111 alone) and used to visualize the presentation of SSX2103–111 in the context of HLA‐A*0201 by fluorescence microscopy. Presentation of SSX2103–111 the context of HLA‐A*0201 was demonstrated for the majority of SK‐Mel‐37, but for only a small fraction (<1%) of Me275 as indicated by a clear membrane‐staining pattern in fluorescence microscopy. The presentation of SSX2103–111 on SK‐Mel37 and Me275, but not the expression of the SSX2 protein correlated with the capability of these cells to stimulate cells of an SSX2103–111‐specific T‐cell clone. MHC‐peptide specific antibodies are a valuable tool for the analysis of antigenic peptides in the context of MHC‐I molecules and for the structural definition of immunodominant epitopes. © 2008 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18688854</pmid><doi>10.1002/ijc.23728</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of cancer, 2008-10, Vol.123 (8), p.1841-1847 |
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| subjects | 3T3 Cells Animals Antibody Specificity Antigen Presentation Antigens, Neoplasm - genetics Antigens, Neoplasm - immunology Biological and medical sciences Breast Neoplasms - genetics Breast Neoplasms - immunology Cell Line, Tumor HLA-A Antigens - immunology HLA-A2 Antigen Humans Immunoglobulin Fragments - immunology Medical sciences Melanoma - genetics Melanoma - immunology MHC‐peptide‐specific antibodies scope Mice Microscopy, Fluorescence Multiple tumors. Solid tumors. Tumors in childhood (general aspects) Neoplasm Proteins - biosynthesis Neoplasm Proteins - genetics Neoplasm Proteins - immunology Peptide Fragments - immunology Polymerase Chain Reaction Repressor Proteins - biosynthesis Repressor Proteins - genetics Repressor Proteins - immunology SSX2 T-Lymphocytes, Cytotoxic - immunology tumor immunology Tumors tumor‐antigen |
| title | Differential presentation of tumor antigen‐derived epitopes by MHC‐class I and antigen‐positive tumor cells |
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