High yield direct 76Br-bromination of monoclonal antibodies using chloramine-T

Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br ( T 1/2=16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean ± max error) of 77 ± 2% was obtained at pH 6.8. In vitro binding of 76Br...

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Veröffentlicht in:	Nuclear medicine and biology 1999-11, Vol.26 (8), p.923-929
Hauptverfasser:	Sundin, Johanna, Tolmachev, Vladimir, Koziorowski, Jacek, Carlsson, Jörgen, Lundqvist, Hans, Welt, Sydney, Larson, Steven, Sundin, Anders
Format:	Artikel
Sprache:	eng
Schlagworte:	76Br A33 Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Biological and medical sciences Bromine Radioisotopes Buffers CAT-labeling Chloramines - chemistry Contrast media. Radiopharmaceuticals Digestion. Liver. Biliary tract. Spleen. Pancreas Hydrogen-Ion Concentration Immunohistochemistry Indicators and Reagents Investigative techniques, diagnostic techniques (general aspects) Isotope Labeling Medical sciences Monoclonal antibodies PET Pharmacology. Drug treatments Radiobromination Radionuclide investigations Radiopharmaceuticals - chemical synthesis Tosyl Compounds - chemistry Tumor Cells, Cultured
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