Metabolic regulation of pathways of carbohydrate oxidation in potato (Solanum tuberosum) tubers
In the present article we evaluate the consequence of tuber-specific expression of yeast invertase, on the pathways of carbohydrate oxidation, in potato (Solanum tuberosum L. cv. Desiree). We analysed the relative rates of glycolysis and the oxidative pentose phosphate pathway that these lines exhib...
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| Veröffentlicht in: | Physiologia Plantarum (København. 1948) 2008-08, Vol.133 (4), p.744-754 |
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| container_title | Physiologia Plantarum (København. 1948) |
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| creator | Centeno, Danilo C Oliver, Sandra N Nunes-Nesi, Adriano Geigenberger, Peter Machado, Daniel N Loureiro, Marcelo Ehlers Silva, Marco A.P Fernie, Alisdair R |
| description | In the present article we evaluate the consequence of tuber-specific expression of yeast invertase, on the pathways of carbohydrate oxidation, in potato (Solanum tuberosum L. cv. Desiree). We analysed the relative rates of glycolysis and the oxidative pentose phosphate pathway that these lines exhibited as well as the relative contributions of the cytochrome and alternative pathways of mitochondrial respiration. Enzymatic and protein abundance analysis revealed concerted upregulation of the glycolytic pathway and of specific enzymes of the tricarboxylic acid cycle and the alternative oxidase but invariant levels of enzymes of the oxidative pentose phosphate pathway and proteins of the cytochrome pathway. When taken together these experiments suggest that the overexpression of a cytosolic invertase (EC 3.2.1.26) results in a general upregulation of carbohydrate oxidation with increased flux through both the glycolytic and oxidative pentose phosphate pathways as well as the cytochrome and alternative pathways of oxidative phosphorylation. Moreover these data suggest that the upregulation of respiration is a consequence of enhanced efficient mitochondrial metabolism. |
| doi_str_mv | 10.1111/j.1399-3054.2008.01109.x |
| format | Article |
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Desiree). We analysed the relative rates of glycolysis and the oxidative pentose phosphate pathway that these lines exhibited as well as the relative contributions of the cytochrome and alternative pathways of mitochondrial respiration. Enzymatic and protein abundance analysis revealed concerted upregulation of the glycolytic pathway and of specific enzymes of the tricarboxylic acid cycle and the alternative oxidase but invariant levels of enzymes of the oxidative pentose phosphate pathway and proteins of the cytochrome pathway. When taken together these experiments suggest that the overexpression of a cytosolic invertase (EC 3.2.1.26) results in a general upregulation of carbohydrate oxidation with increased flux through both the glycolytic and oxidative pentose phosphate pathways as well as the cytochrome and alternative pathways of oxidative phosphorylation. 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Psychology ; Glycolysis ; Immunoblotting ; Metabolic Networks and Pathways ; Mitochondria - metabolism ; Molecular and cellular biology ; Oxidation-Reduction ; Pentose Phosphate Pathway ; Plant Tubers - enzymology ; Plant Tubers - metabolism ; Plants, Genetically Modified ; Saccharomyces cerevisiae - enzymology ; Signal transduction ; Solanum tuberosum ; Solanum tuberosum - cytology ; Solanum tuberosum - genetics ; Solanum tuberosum - metabolism</subject><ispartof>Physiologia Plantarum (København. 1948), 2008-08, Vol.133 (4), p.744-754</ispartof><rights>Physiologia Plantarum 2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4909-2e3dc313d13b7c4979e044549c63c51363bd32ad1b45594958b6d02de5078d603</citedby><cites>FETCH-LOGICAL-c4909-2e3dc313d13b7c4979e044549c63c51363bd32ad1b45594958b6d02de5078d603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1399-3054.2008.01109.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1399-3054.2008.01109.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,1416,23928,23929,25138,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20592689$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18494735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Centeno, Danilo C</creatorcontrib><creatorcontrib>Oliver, Sandra N</creatorcontrib><creatorcontrib>Nunes-Nesi, Adriano</creatorcontrib><creatorcontrib>Geigenberger, Peter</creatorcontrib><creatorcontrib>Machado, Daniel N</creatorcontrib><creatorcontrib>Loureiro, Marcelo Ehlers</creatorcontrib><creatorcontrib>Silva, Marco A.P</creatorcontrib><creatorcontrib>Fernie, Alisdair R</creatorcontrib><title>Metabolic regulation of pathways of carbohydrate oxidation in potato (Solanum tuberosum) tubers</title><title>Physiologia Plantarum (København. 1948)</title><addtitle>Physiol Plant</addtitle><description>In the present article we evaluate the consequence of tuber-specific expression of yeast invertase, on the pathways of carbohydrate oxidation, in potato (Solanum tuberosum L. cv. Desiree). We analysed the relative rates of glycolysis and the oxidative pentose phosphate pathway that these lines exhibited as well as the relative contributions of the cytochrome and alternative pathways of mitochondrial respiration. Enzymatic and protein abundance analysis revealed concerted upregulation of the glycolytic pathway and of specific enzymes of the tricarboxylic acid cycle and the alternative oxidase but invariant levels of enzymes of the oxidative pentose phosphate pathway and proteins of the cytochrome pathway. When taken together these experiments suggest that the overexpression of a cytosolic invertase (EC 3.2.1.26) results in a general upregulation of carbohydrate oxidation with increased flux through both the glycolytic and oxidative pentose phosphate pathways as well as the cytochrome and alternative pathways of oxidative phosphorylation. Moreover these data suggest that the upregulation of respiration is a consequence of enhanced efficient mitochondrial metabolism.</description><subject>beta-Fructofuranosidase - metabolism</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate Metabolism</subject><subject>Cell physiology</subject><subject>Cell Respiration</subject><subject>Citric Acid Cycle</subject><subject>Cytochromes - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycolysis</subject><subject>Immunoblotting</subject><subject>Metabolic Networks and Pathways</subject><subject>Mitochondria - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Oxidation-Reduction</subject><subject>Pentose Phosphate Pathway</subject><subject>Plant Tubers - enzymology</subject><subject>Plant Tubers - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Signal transduction</subject><subject>Solanum tuberosum</subject><subject>Solanum tuberosum - cytology</subject><subject>Solanum tuberosum - genetics</subject><subject>Solanum tuberosum - metabolism</subject><issn>0031-9317</issn><issn>1399-3054</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EotPCK0A2oLJIuP5NvOgCVVBAQxlRKpaWYztthmQ82Ik68_Y4ZDQswRtf2d-5Pj4XoQxDgdN6uy4wlTKnwFlBAKoCMAZZ7B6hxfHiMVoAUJxLissTdBrjGgALgclTdIIrJllJ-QKpL27Qte9akwV3N3Z6aP0m80221cP9g97HqTY61P5-b4MeXOZ3rZ2pdpNt_aAHn53f-E5vxj4bxtoFH8f-zVzGZ-hJo7vonh_2M3T74f33y4_58uvVp8t3y9wwCTInjlpDMbWY1mU6KqUDxjiTRlDDMRW0tpRoi2vGuWSSV7WwQKzjUFZWAD1Dr-e-2-B_jS4Oqm-jcV2y5fwYlZCMlZjRf4IEqkoQiRNYzaBJH4rBNWob2l6HvcKgpimotZrCVlPYapqC-jMFtUvSF4c3xrp39q_wEHsCXh0AHY3umqA3po1HjgCXRFQycRcz99B2bv_fBtRqtZyqpM9nfRsHtzvqdfipRElLrn5cXymxEp-vvy2lYol_OfON9krfheTp9oYApgApEYIp_Q2uVbqk</recordid><startdate>200808</startdate><enddate>200808</enddate><creator>Centeno, Danilo C</creator><creator>Oliver, Sandra N</creator><creator>Nunes-Nesi, Adriano</creator><creator>Geigenberger, Peter</creator><creator>Machado, Daniel N</creator><creator>Loureiro, Marcelo Ehlers</creator><creator>Silva, Marco A.P</creator><creator>Fernie, Alisdair R</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200808</creationdate><title>Metabolic regulation of pathways of carbohydrate oxidation in potato (Solanum tuberosum) tubers</title><author>Centeno, Danilo C ; Oliver, Sandra N ; Nunes-Nesi, Adriano ; Geigenberger, Peter ; Machado, Daniel N ; Loureiro, Marcelo Ehlers ; Silva, Marco A.P ; Fernie, Alisdair R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4909-2e3dc313d13b7c4979e044549c63c51363bd32ad1b45594958b6d02de5078d603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>beta-Fructofuranosidase - metabolism</topic><topic>Biological and medical sciences</topic><topic>Carbohydrate Metabolism</topic><topic>Cell physiology</topic><topic>Cell Respiration</topic><topic>Citric Acid Cycle</topic><topic>Cytochromes - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycolysis</topic><topic>Immunoblotting</topic><topic>Metabolic Networks and Pathways</topic><topic>Mitochondria - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Oxidation-Reduction</topic><topic>Pentose Phosphate Pathway</topic><topic>Plant Tubers - enzymology</topic><topic>Plant Tubers - metabolism</topic><topic>Plants, Genetically Modified</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Signal transduction</topic><topic>Solanum tuberosum</topic><topic>Solanum tuberosum - cytology</topic><topic>Solanum tuberosum - genetics</topic><topic>Solanum tuberosum - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Centeno, Danilo C</creatorcontrib><creatorcontrib>Oliver, Sandra N</creatorcontrib><creatorcontrib>Nunes-Nesi, Adriano</creatorcontrib><creatorcontrib>Geigenberger, Peter</creatorcontrib><creatorcontrib>Machado, Daniel N</creatorcontrib><creatorcontrib>Loureiro, Marcelo Ehlers</creatorcontrib><creatorcontrib>Silva, Marco A.P</creatorcontrib><creatorcontrib>Fernie, Alisdair R</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Physiologia Plantarum (København. 1948)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Centeno, Danilo C</au><au>Oliver, Sandra N</au><au>Nunes-Nesi, Adriano</au><au>Geigenberger, Peter</au><au>Machado, Daniel N</au><au>Loureiro, Marcelo Ehlers</au><au>Silva, Marco A.P</au><au>Fernie, Alisdair R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic regulation of pathways of carbohydrate oxidation in potato (Solanum tuberosum) tubers</atitle><jtitle>Physiologia Plantarum (København. 1948)</jtitle><addtitle>Physiol Plant</addtitle><date>2008-08</date><risdate>2008</risdate><volume>133</volume><issue>4</issue><spage>744</spage><epage>754</epage><pages>744-754</pages><issn>0031-9317</issn><eissn>1399-3054</eissn><coden>PHPLAI</coden><abstract>In the present article we evaluate the consequence of tuber-specific expression of yeast invertase, on the pathways of carbohydrate oxidation, in potato (Solanum tuberosum L. cv. Desiree). We analysed the relative rates of glycolysis and the oxidative pentose phosphate pathway that these lines exhibited as well as the relative contributions of the cytochrome and alternative pathways of mitochondrial respiration. Enzymatic and protein abundance analysis revealed concerted upregulation of the glycolytic pathway and of specific enzymes of the tricarboxylic acid cycle and the alternative oxidase but invariant levels of enzymes of the oxidative pentose phosphate pathway and proteins of the cytochrome pathway. When taken together these experiments suggest that the overexpression of a cytosolic invertase (EC 3.2.1.26) results in a general upregulation of carbohydrate oxidation with increased flux through both the glycolytic and oxidative pentose phosphate pathways as well as the cytochrome and alternative pathways of oxidative phosphorylation. 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| ispartof | Physiologia Plantarum (København. 1948), 2008-08, Vol.133 (4), p.744-754 |
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| subjects | beta-Fructofuranosidase - metabolism Biological and medical sciences Carbohydrate Metabolism Cell physiology Cell Respiration Citric Acid Cycle Cytochromes - metabolism Fundamental and applied biological sciences. Psychology Glycolysis Immunoblotting Metabolic Networks and Pathways Mitochondria - metabolism Molecular and cellular biology Oxidation-Reduction Pentose Phosphate Pathway Plant Tubers - enzymology Plant Tubers - metabolism Plants, Genetically Modified Saccharomyces cerevisiae - enzymology Signal transduction Solanum tuberosum Solanum tuberosum - cytology Solanum tuberosum - genetics Solanum tuberosum - metabolism |
| title | Metabolic regulation of pathways of carbohydrate oxidation in potato (Solanum tuberosum) tubers |
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