First Detection of Leishmania infantum in Phlebotomus (Larroussius) major (Diptera: Psychodidae) from Iran
Ghir-Karzin district is one of the most important endemic foci of visceral leishmaniaisis (VL) in Fars province of southern Iran. To identify the vector(s) of Leishmania in this focus, a total of 2,539 sand flies were collected during June to September 2005. Eleven species (six Phlebotomus and five...
Gespeichert in:
| Veröffentlicht in: | Journal of medical entomology 2008-07, Vol.45 (4), p.726-731 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 731 |
|---|---|
| container_issue | 4 |
| container_start_page | 726 |
| container_title | Journal of medical entomology |
| container_volume | 45 |
| creator | Azizi, K. Rassi, Y. Javadian, E. Motazedian, M. H. Asgari, Q. Yaghoobi-Ershadi, M. R. |
| description | Ghir-Karzin district is one of the most important endemic foci of visceral leishmaniaisis (VL) in Fars province of southern Iran. To identify the vector(s) of Leishmania in this focus, a total of 2,539 sand flies were collected during June to September 2005. Eleven species (six Phlebotomus and five Sergentomyia) were identified, of which Phlebotomus papatasi Scopoli and Phlebotomus major Annandale were the first (37.4%) and third (11.2%) most common species, respectively. Natural leptomonad infections were observed in two P. papatasi (4.25%) and three P. major (6.65%) specimens on dissection and microscopic examination. Using a Leishmania genus–specific standard polymerase chain reaction (PCR; primers RV1-RV2) and a species-specific nested-PCR (primers LINR4, LIN17, and LIN 19), Leishmania infantum and Leishmania major kinetoplast minicircle DNA was detected in 6 of 72 P. major (8.3%) and 4 of 65 P. papatasi (6.1%), respectively. This is the first detection of L. infantum in P. major, implicating this sand fly as a probable vector of VL in Iran. |
| doi_str_mv | 10.1603/0022-2585%282008%2945%5B726%3AFDOLII%5D2.0.CO%3B2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_69445669</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69445669</sourcerecordid><originalsourceid>FETCH-LOGICAL-b1804-c0657552595aa77220c6d078817849f7b133ba340bafa9cd77336f2d0fe5e7f03</originalsourceid><addsrcrecordid>eNpFkUFv0zAYhiMEYmXwF8CXoO3Q8tmOY5vb1lKoFCmTYGfLSWzqqomLnRz273FoN0724fHr9_ueLJMYVrgE-gWAkCVhguVEEACRE1mwnN1zUub0brupq90uZxuygtW6zuk9eZUtsKRiSSQRr7PFy_ur7F2MB0gRuJBvsyssOC4ELxbZYetCHNHGjKYdnR-Qt6gyLu57PTiN3GD1ME59uqCH_dE0fvT9FNFNpUPwU4xuireo1wcf0M3GnUYT9Ff0EJ_ave9cp80tssH3aBf08D57Y_Uxmg-X8zp73H77tf6xrOrvu_VdtWywgGLZQsk4Y4RJpjXnhEBbdsCFwFwU0vIGU9poWkCjrZZtxzmlpSUdWMMMt0Cvs8_n3FPwfyYTR9W72JrjUQ8mVValLApWljKBHy_g1PSmU6fgeh2e1PN2EpBfAB1bfbRpiNbFF45AiTETc9CnM2e1V_p3SMzjTwKYAkgsU99E1Geicd4P5v9XoGbTajalZlPqbFrNptU_0-rZtEqmFah1rZJp-hdbCZoL</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69445669</pqid></control><display><type>article</type><title>First Detection of Leishmania infantum in Phlebotomus (Larroussius) major (Diptera: Psychodidae) from Iran</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>BioOne Complete</source><creator>Azizi, K. ; Rassi, Y. ; Javadian, E. ; Motazedian, M. H. ; Asgari, Q. ; Yaghoobi-Ershadi, M. R.</creator><creatorcontrib>Azizi, K. ; Rassi, Y. ; Javadian, E. ; Motazedian, M. H. ; Asgari, Q. ; Yaghoobi-Ershadi, M. R.</creatorcontrib><description>Ghir-Karzin district is one of the most important endemic foci of visceral leishmaniaisis (VL) in Fars province of southern Iran. To identify the vector(s) of Leishmania in this focus, a total of 2,539 sand flies were collected during June to September 2005. Eleven species (six Phlebotomus and five Sergentomyia) were identified, of which Phlebotomus papatasi Scopoli and Phlebotomus major Annandale were the first (37.4%) and third (11.2%) most common species, respectively. Natural leptomonad infections were observed in two P. papatasi (4.25%) and three P. major (6.65%) specimens on dissection and microscopic examination. Using a Leishmania genus–specific standard polymerase chain reaction (PCR; primers RV1-RV2) and a species-specific nested-PCR (primers LINR4, LIN17, and LIN 19), Leishmania infantum and Leishmania major kinetoplast minicircle DNA was detected in 6 of 72 P. major (8.3%) and 4 of 65 P. papatasi (6.1%), respectively. This is the first detection of L. infantum in P. major, implicating this sand fly as a probable vector of VL in Iran.</description><identifier>ISSN: 0022-2585</identifier><identifier>EISSN: 1938-2928</identifier><identifier>DOI: 10.1603/0022-2585%282008%2945%5B726%3AFDOLII%5D2.0.CO%3B2</identifier><identifier>PMID: 18714874</identifier><identifier>CODEN: JMENA6</identifier><language>eng</language><publisher>Lanham, MD: Entomological Society of America</publisher><subject>Animals ; Biological and medical sciences ; diagnostic techniques ; disease detection ; DNA - genetics ; DNA - isolation & purification ; DNA, Protozoan - genetics ; DNA, Protozoan - isolation & purification ; epidemiological studies ; Fundamental and applied biological sciences. Psychology ; human diseases ; infection ; insect surveys ; insect vectors ; Insecta ; Invertebrates ; Iran ; Leishmania infantum ; Leishmania infantum - genetics ; Leishmania infantum - isolation & purification ; Leishmania major ; Medically important nuisances and vectors, pests of stored products and materials: population survey and control ; microscopy ; nested polymerase chain reaction ; pathogen identification ; Phlebotomus ; Phlebotomus - classification ; Phlebotomus - parasitology ; Phlebotomus major ; Phlebotomus papatasi ; Polymerase Chain Reaction ; risk assessment ; Sergentomyia ; Species Specificity ; VECTOR/PATHOGEN/HOST INTERACTION, TRANSMISSION ; Vectors. Intermediate hosts ; visceral leishmaniasis</subject><ispartof>Journal of medical entomology, 2008-07, Vol.45 (4), p.726-731</ispartof><rights>Entomological Society of America</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1603/0022-2585%282008%2945%5B726%3AFDOLII%5D2.0.CO%3B2$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,776,780,26955,27901,27902,52338</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20611589$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18714874$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Azizi, K.</creatorcontrib><creatorcontrib>Rassi, Y.</creatorcontrib><creatorcontrib>Javadian, E.</creatorcontrib><creatorcontrib>Motazedian, M. H.</creatorcontrib><creatorcontrib>Asgari, Q.</creatorcontrib><creatorcontrib>Yaghoobi-Ershadi, M. R.</creatorcontrib><title>First Detection of Leishmania infantum in Phlebotomus (Larroussius) major (Diptera: Psychodidae) from Iran</title><title>Journal of medical entomology</title><addtitle>J Med Entomol</addtitle><description>Ghir-Karzin district is one of the most important endemic foci of visceral leishmaniaisis (VL) in Fars province of southern Iran. To identify the vector(s) of Leishmania in this focus, a total of 2,539 sand flies were collected during June to September 2005. Eleven species (six Phlebotomus and five Sergentomyia) were identified, of which Phlebotomus papatasi Scopoli and Phlebotomus major Annandale were the first (37.4%) and third (11.2%) most common species, respectively. Natural leptomonad infections were observed in two P. papatasi (4.25%) and three P. major (6.65%) specimens on dissection and microscopic examination. Using a Leishmania genus–specific standard polymerase chain reaction (PCR; primers RV1-RV2) and a species-specific nested-PCR (primers LINR4, LIN17, and LIN 19), Leishmania infantum and Leishmania major kinetoplast minicircle DNA was detected in 6 of 72 P. major (8.3%) and 4 of 65 P. papatasi (6.1%), respectively. This is the first detection of L. infantum in P. major, implicating this sand fly as a probable vector of VL in Iran.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>diagnostic techniques</subject><subject>disease detection</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>DNA, Protozoan - genetics</subject><subject>DNA, Protozoan - isolation & purification</subject><subject>epidemiological studies</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>human diseases</subject><subject>infection</subject><subject>insect surveys</subject><subject>insect vectors</subject><subject>Insecta</subject><subject>Invertebrates</subject><subject>Iran</subject><subject>Leishmania infantum</subject><subject>Leishmania infantum - genetics</subject><subject>Leishmania infantum - isolation & purification</subject><subject>Leishmania major</subject><subject>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</subject><subject>microscopy</subject><subject>nested polymerase chain reaction</subject><subject>pathogen identification</subject><subject>Phlebotomus</subject><subject>Phlebotomus - classification</subject><subject>Phlebotomus - parasitology</subject><subject>Phlebotomus major</subject><subject>Phlebotomus papatasi</subject><subject>Polymerase Chain Reaction</subject><subject>risk assessment</subject><subject>Sergentomyia</subject><subject>Species Specificity</subject><subject>VECTOR/PATHOGEN/HOST INTERACTION, TRANSMISSION</subject><subject>Vectors. Intermediate hosts</subject><subject>visceral leishmaniasis</subject><issn>0022-2585</issn><issn>1938-2928</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkUFv0zAYhiMEYmXwF8CXoO3Q8tmOY5vb1lKoFCmTYGfLSWzqqomLnRz273FoN0724fHr9_ueLJMYVrgE-gWAkCVhguVEEACRE1mwnN1zUub0brupq90uZxuygtW6zuk9eZUtsKRiSSQRr7PFy_ur7F2MB0gRuJBvsyssOC4ELxbZYetCHNHGjKYdnR-Qt6gyLu57PTiN3GD1ME59uqCH_dE0fvT9FNFNpUPwU4xuireo1wcf0M3GnUYT9Ff0EJ_ave9cp80tssH3aBf08D57Y_Uxmg-X8zp73H77tf6xrOrvu_VdtWywgGLZQsk4Y4RJpjXnhEBbdsCFwFwU0vIGU9poWkCjrZZtxzmlpSUdWMMMt0Cvs8_n3FPwfyYTR9W72JrjUQ8mVValLApWljKBHy_g1PSmU6fgeh2e1PN2EpBfAB1bfbRpiNbFF45AiTETc9CnM2e1V_p3SMzjTwKYAkgsU99E1Geicd4P5v9XoGbTajalZlPqbFrNptU_0-rZtEqmFah1rZJp-hdbCZoL</recordid><startdate>200807</startdate><enddate>200807</enddate><creator>Azizi, K.</creator><creator>Rassi, Y.</creator><creator>Javadian, E.</creator><creator>Motazedian, M. H.</creator><creator>Asgari, Q.</creator><creator>Yaghoobi-Ershadi, M. R.</creator><general>Entomological Society of America</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200807</creationdate><title>First Detection of Leishmania infantum in Phlebotomus (Larroussius) major (Diptera: Psychodidae) from Iran</title><author>Azizi, K. ; Rassi, Y. ; Javadian, E. ; Motazedian, M. H. ; Asgari, Q. ; Yaghoobi-Ershadi, M. R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b1804-c0657552595aa77220c6d078817849f7b133ba340bafa9cd77336f2d0fe5e7f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>diagnostic techniques</topic><topic>disease detection</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>DNA, Protozoan - genetics</topic><topic>DNA, Protozoan - isolation & purification</topic><topic>epidemiological studies</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>human diseases</topic><topic>infection</topic><topic>insect surveys</topic><topic>insect vectors</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>Iran</topic><topic>Leishmania infantum</topic><topic>Leishmania infantum - genetics</topic><topic>Leishmania infantum - isolation & purification</topic><topic>Leishmania major</topic><topic>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</topic><topic>microscopy</topic><topic>nested polymerase chain reaction</topic><topic>pathogen identification</topic><topic>Phlebotomus</topic><topic>Phlebotomus - classification</topic><topic>Phlebotomus - parasitology</topic><topic>Phlebotomus major</topic><topic>Phlebotomus papatasi</topic><topic>Polymerase Chain Reaction</topic><topic>risk assessment</topic><topic>Sergentomyia</topic><topic>Species Specificity</topic><topic>VECTOR/PATHOGEN/HOST INTERACTION, TRANSMISSION</topic><topic>Vectors. Intermediate hosts</topic><topic>visceral leishmaniasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Azizi, K.</creatorcontrib><creatorcontrib>Rassi, Y.</creatorcontrib><creatorcontrib>Javadian, E.</creatorcontrib><creatorcontrib>Motazedian, M. H.</creatorcontrib><creatorcontrib>Asgari, Q.</creatorcontrib><creatorcontrib>Yaghoobi-Ershadi, M. R.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Azizi, K.</au><au>Rassi, Y.</au><au>Javadian, E.</au><au>Motazedian, M. H.</au><au>Asgari, Q.</au><au>Yaghoobi-Ershadi, M. R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First Detection of Leishmania infantum in Phlebotomus (Larroussius) major (Diptera: Psychodidae) from Iran</atitle><jtitle>Journal of medical entomology</jtitle><addtitle>J Med Entomol</addtitle><date>2008-07</date><risdate>2008</risdate><volume>45</volume><issue>4</issue><spage>726</spage><epage>731</epage><pages>726-731</pages><issn>0022-2585</issn><eissn>1938-2928</eissn><coden>JMENA6</coden><abstract>Ghir-Karzin district is one of the most important endemic foci of visceral leishmaniaisis (VL) in Fars province of southern Iran. To identify the vector(s) of Leishmania in this focus, a total of 2,539 sand flies were collected during June to September 2005. Eleven species (six Phlebotomus and five Sergentomyia) were identified, of which Phlebotomus papatasi Scopoli and Phlebotomus major Annandale were the first (37.4%) and third (11.2%) most common species, respectively. Natural leptomonad infections were observed in two P. papatasi (4.25%) and three P. major (6.65%) specimens on dissection and microscopic examination. Using a Leishmania genus–specific standard polymerase chain reaction (PCR; primers RV1-RV2) and a species-specific nested-PCR (primers LINR4, LIN17, and LIN 19), Leishmania infantum and Leishmania major kinetoplast minicircle DNA was detected in 6 of 72 P. major (8.3%) and 4 of 65 P. papatasi (6.1%), respectively. This is the first detection of L. infantum in P. major, implicating this sand fly as a probable vector of VL in Iran.</abstract><cop>Lanham, MD</cop><pub>Entomological Society of America</pub><pmid>18714874</pmid><doi>10.1603/0022-2585%282008%2945%5B726%3AFDOLII%5D2.0.CO%3B2</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2585 |
| ispartof | Journal of medical entomology, 2008-07, Vol.45 (4), p.726-731 |
| issn | 0022-2585 1938-2928 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69445669 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; BioOne Complete |
| subjects | Animals Biological and medical sciences diagnostic techniques disease detection DNA - genetics DNA - isolation & purification DNA, Protozoan - genetics DNA, Protozoan - isolation & purification epidemiological studies Fundamental and applied biological sciences. Psychology human diseases infection insect surveys insect vectors Insecta Invertebrates Iran Leishmania infantum Leishmania infantum - genetics Leishmania infantum - isolation & purification Leishmania major Medically important nuisances and vectors, pests of stored products and materials: population survey and control microscopy nested polymerase chain reaction pathogen identification Phlebotomus Phlebotomus - classification Phlebotomus - parasitology Phlebotomus major Phlebotomus papatasi Polymerase Chain Reaction risk assessment Sergentomyia Species Specificity VECTOR/PATHOGEN/HOST INTERACTION, TRANSMISSION Vectors. Intermediate hosts visceral leishmaniasis |
| title | First Detection of Leishmania infantum in Phlebotomus (Larroussius) major (Diptera: Psychodidae) from Iran |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T11%3A49%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=First%20Detection%20of%20Leishmania%20infantum%20in%20Phlebotomus%20(Larroussius)%20major%20(Diptera:%20Psychodidae)%20from%20Iran&rft.jtitle=Journal%20of%20medical%20entomology&rft.au=Azizi,%20K.&rft.date=2008-07&rft.volume=45&rft.issue=4&rft.spage=726&rft.epage=731&rft.pages=726-731&rft.issn=0022-2585&rft.eissn=1938-2928&rft.coden=JMENA6&rft_id=info:doi/10.1603/0022-2585%25282008%252945%255B726%253AFDOLII%255D2.0.CO%253B2&rft_dat=%3Cproquest_pubme%3E69445669%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69445669&rft_id=info:pmid/18714874&rfr_iscdi=true |