Molecular characterization of calcitonin gene-related peptide (CGRP) related peptides (CGRP, amylin, adrenomedullin and adrenomedullin-2/intermedin) in goldfish ( Carassius auratus): Cloning and distribution
To further characterize the structure and function of calcitonin gene-related peptide (CGRP) related peptides in fish, we have cloned cDNA sequences for CGRP, amylin, adrenomedullin (AM) and adrenomedullin-2/intermedin (IMD) in goldfish ( Carassius auratus) and examined their tissue distribution. CG...
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| Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2008-09, Vol.29 (9), p.1534-1543 |
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| creator | Martínez-Álvarez, R.M. Volkoff, H. Cueto, J.A. Muñoz Delgado, M.J. |
| description | To further characterize the structure and function of calcitonin gene-related peptide (CGRP) related peptides in fish, we have cloned cDNA sequences for CGRP, amylin, adrenomedullin (AM) and adrenomedullin-2/intermedin (IMD) in goldfish (
Carassius auratus) and examined their tissue distribution. CGRP, amylin, AM and IMD cDNAs were isolated by reverse transcription (RT) and rapid amplification of cDNA ends (RACE). The cloned sequences contain the complete four mature peptides, which present a high degree of identity with mature peptide sequences from other fish. Phylogenetic analyses show that goldfish AM and IMD form a sub-family within the CGRP-related peptides that is distinct from the CGRP/amylin sub-family. The distribution of goldfish CGRP-like peptides mRNA expression in different tissues and within the brain was studied by RT-PCR. CGRP, IMD and AM are detected throughout the brain, in pituitary and in most peripheral tissues examined. Amylin mRNA is mostly expressed in the brain, in particular posterior brain, optic tectum and hypothalamus, but is also present in pituitary, gonad, kidney and muscle. Our results suggest that goldfish CGRP, amylin, AM and IMD are conserved peptides that show the typical structure characteristics present in their mammalian counterparts. The widespread distributions of CGRP, AM and IMD suggest that these peptides could be involved in the regulation of many diverse physiological functions in fish. Amylin mRNA distribution suggests possible new roles for this peptide in teleosts, including the control of reproduction. |
| doi_str_mv | 10.1016/j.peptides.2008.04.013 |
| format | Article |
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Carassius auratus) and examined their tissue distribution. CGRP, amylin, AM and IMD cDNAs were isolated by reverse transcription (RT) and rapid amplification of cDNA ends (RACE). The cloned sequences contain the complete four mature peptides, which present a high degree of identity with mature peptide sequences from other fish. Phylogenetic analyses show that goldfish AM and IMD form a sub-family within the CGRP-related peptides that is distinct from the CGRP/amylin sub-family. The distribution of goldfish CGRP-like peptides mRNA expression in different tissues and within the brain was studied by RT-PCR. CGRP, IMD and AM are detected throughout the brain, in pituitary and in most peripheral tissues examined. Amylin mRNA is mostly expressed in the brain, in particular posterior brain, optic tectum and hypothalamus, but is also present in pituitary, gonad, kidney and muscle. Our results suggest that goldfish CGRP, amylin, AM and IMD are conserved peptides that show the typical structure characteristics present in their mammalian counterparts. The widespread distributions of CGRP, AM and IMD suggest that these peptides could be involved in the regulation of many diverse physiological functions in fish. Amylin mRNA distribution suggests possible new roles for this peptide in teleosts, including the control of reproduction.</description><identifier>ISSN: 0196-9781</identifier><identifier>EISSN: 1873-5169</identifier><identifier>DOI: 10.1016/j.peptides.2008.04.013</identifier><identifier>PMID: 18539360</identifier><identifier>CODEN: PPTDD5</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adrenomedullin ; Adrenomedullin - isolation & purification ; Adrenomedullin-2/intermedin ; Amino Acid Sequence ; Amylin ; Amyloid - isolation & purification ; Animals ; Biological and medical sciences ; Calcitonin Gene-Related Peptide - isolation & purification ; Carassius auratus ; CGRP ; Cloning ; Cloning, Molecular ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Goldfish ; Humans ; Islet Amyloid Polypeptide ; Molecular Sequence Data ; Neuropeptides - isolation & purification ; RNA, Messenger - metabolism ; Sequence Alignment ; Teleostei ; Tissue Distribution ; Vertebrates: endocrinology</subject><ispartof>Peptides (New York, N.Y. : 1980), 2008-09, Vol.29 (9), p.1534-1543</ispartof><rights>2008 Elsevier Inc.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-d14bd13221939db73f260706f06541ce10eea38f61aa57959ab5614b4b4f5d393</citedby><cites>FETCH-LOGICAL-c493t-d14bd13221939db73f260706f06541ce10eea38f61aa57959ab5614b4b4f5d393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.peptides.2008.04.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20674443$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18539360$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martínez-Álvarez, R.M.</creatorcontrib><creatorcontrib>Volkoff, H.</creatorcontrib><creatorcontrib>Cueto, J.A. Muñoz</creatorcontrib><creatorcontrib>Delgado, M.J.</creatorcontrib><title>Molecular characterization of calcitonin gene-related peptide (CGRP) related peptides (CGRP, amylin, adrenomedullin and adrenomedullin-2/intermedin) in goldfish ( Carassius auratus): Cloning and distribution</title><title>Peptides (New York, N.Y. : 1980)</title><addtitle>Peptides</addtitle><description>To further characterize the structure and function of calcitonin gene-related peptide (CGRP) related peptides in fish, we have cloned cDNA sequences for CGRP, amylin, adrenomedullin (AM) and adrenomedullin-2/intermedin (IMD) in goldfish (
Carassius auratus) and examined their tissue distribution. CGRP, amylin, AM and IMD cDNAs were isolated by reverse transcription (RT) and rapid amplification of cDNA ends (RACE). The cloned sequences contain the complete four mature peptides, which present a high degree of identity with mature peptide sequences from other fish. Phylogenetic analyses show that goldfish AM and IMD form a sub-family within the CGRP-related peptides that is distinct from the CGRP/amylin sub-family. The distribution of goldfish CGRP-like peptides mRNA expression in different tissues and within the brain was studied by RT-PCR. CGRP, IMD and AM are detected throughout the brain, in pituitary and in most peripheral tissues examined. Amylin mRNA is mostly expressed in the brain, in particular posterior brain, optic tectum and hypothalamus, but is also present in pituitary, gonad, kidney and muscle. Our results suggest that goldfish CGRP, amylin, AM and IMD are conserved peptides that show the typical structure characteristics present in their mammalian counterparts. The widespread distributions of CGRP, AM and IMD suggest that these peptides could be involved in the regulation of many diverse physiological functions in fish. Amylin mRNA distribution suggests possible new roles for this peptide in teleosts, including the control of reproduction.</description><subject>Adrenomedullin</subject><subject>Adrenomedullin - isolation & purification</subject><subject>Adrenomedullin-2/intermedin</subject><subject>Amino Acid Sequence</subject><subject>Amylin</subject><subject>Amyloid - isolation & purification</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcitonin Gene-Related Peptide - isolation & purification</subject><subject>Carassius auratus</subject><subject>CGRP</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Goldfish</subject><subject>Humans</subject><subject>Islet Amyloid Polypeptide</subject><subject>Molecular Sequence Data</subject><subject>Neuropeptides - isolation & purification</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><subject>Teleostei</subject><subject>Tissue Distribution</subject><subject>Vertebrates: endocrinology</subject><issn>0196-9781</issn><issn>1873-5169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUdGK1TAQLaK419VfWPKi7AXbTdo0bXxSiq7CiiL6HNJkuptLbnJNUmH9SX_J1FsV9mWZh2GGM3MO5xTFGcEVwYRd7KoDHJLREKsa477CtMKkeVBsSN81ZUsYf1hsMOGs5F1PToonMe4wxpTy_nFxQvq24Q3Dm-LXR29BzVYGpG5kkCpBMD9lMt4hPyElrTLJO-PQNTgoA1iZQKOVHJ0Pl18-b9GddTzuXyK5v7XG5a4DOL8HPds8I-n0nVVZXxiXqfNs3BYtdN7qycQbdI6GrCtGM0ck5yDTHLev0GAXUdd_XmkTUzDjvIh-WjyapI3wbO2nxbd3b78O78urT5cfhjdXpaK8SaUmdNSkqWvCG67HrplqhjvMJsxaShQQDCCbfmJEyrbjLZdjy_JNrqnV2bvT4sXx7yH47zPEJPYmKrBWOvBzFIzThrddey-Q8B7XHNMMZEegCj7GAJM4BLOX4VYQLJbMxU789VcsmQtMRc48H56tDPOY_ft_toacAc9XgIw50ClIp0z8h6sx6yily6PXRxxk434YCCIqA07lTAKoJLQ392n5DSK50TQ</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Martínez-Álvarez, R.M.</creator><creator>Volkoff, H.</creator><creator>Cueto, J.A. Muñoz</creator><creator>Delgado, M.J.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>Molecular characterization of calcitonin gene-related peptide (CGRP) related peptides (CGRP, amylin, adrenomedullin and adrenomedullin-2/intermedin) in goldfish ( Carassius auratus): Cloning and distribution</title><author>Martínez-Álvarez, R.M. ; Volkoff, H. ; Cueto, J.A. Muñoz ; Delgado, M.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-d14bd13221939db73f260706f06541ce10eea38f61aa57959ab5614b4b4f5d393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adrenomedullin</topic><topic>Adrenomedullin - isolation & purification</topic><topic>Adrenomedullin-2/intermedin</topic><topic>Amino Acid Sequence</topic><topic>Amylin</topic><topic>Amyloid - isolation & purification</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcitonin Gene-Related Peptide - isolation & purification</topic><topic>Carassius auratus</topic><topic>CGRP</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Goldfish</topic><topic>Humans</topic><topic>Islet Amyloid Polypeptide</topic><topic>Molecular Sequence Data</topic><topic>Neuropeptides - isolation & purification</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><topic>Teleostei</topic><topic>Tissue Distribution</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martínez-Álvarez, R.M.</creatorcontrib><creatorcontrib>Volkoff, H.</creatorcontrib><creatorcontrib>Cueto, J.A. Muñoz</creatorcontrib><creatorcontrib>Delgado, M.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Peptides (New York, N.Y. : 1980)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martínez-Álvarez, R.M.</au><au>Volkoff, H.</au><au>Cueto, J.A. Muñoz</au><au>Delgado, M.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of calcitonin gene-related peptide (CGRP) related peptides (CGRP, amylin, adrenomedullin and adrenomedullin-2/intermedin) in goldfish ( Carassius auratus): Cloning and distribution</atitle><jtitle>Peptides (New York, N.Y. : 1980)</jtitle><addtitle>Peptides</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>29</volume><issue>9</issue><spage>1534</spage><epage>1543</epage><pages>1534-1543</pages><issn>0196-9781</issn><eissn>1873-5169</eissn><coden>PPTDD5</coden><abstract>To further characterize the structure and function of calcitonin gene-related peptide (CGRP) related peptides in fish, we have cloned cDNA sequences for CGRP, amylin, adrenomedullin (AM) and adrenomedullin-2/intermedin (IMD) in goldfish (
Carassius auratus) and examined their tissue distribution. CGRP, amylin, AM and IMD cDNAs were isolated by reverse transcription (RT) and rapid amplification of cDNA ends (RACE). The cloned sequences contain the complete four mature peptides, which present a high degree of identity with mature peptide sequences from other fish. Phylogenetic analyses show that goldfish AM and IMD form a sub-family within the CGRP-related peptides that is distinct from the CGRP/amylin sub-family. The distribution of goldfish CGRP-like peptides mRNA expression in different tissues and within the brain was studied by RT-PCR. CGRP, IMD and AM are detected throughout the brain, in pituitary and in most peripheral tissues examined. Amylin mRNA is mostly expressed in the brain, in particular posterior brain, optic tectum and hypothalamus, but is also present in pituitary, gonad, kidney and muscle. Our results suggest that goldfish CGRP, amylin, AM and IMD are conserved peptides that show the typical structure characteristics present in their mammalian counterparts. The widespread distributions of CGRP, AM and IMD suggest that these peptides could be involved in the regulation of many diverse physiological functions in fish. Amylin mRNA distribution suggests possible new roles for this peptide in teleosts, including the control of reproduction.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>18539360</pmid><doi>10.1016/j.peptides.2008.04.013</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Peptides (New York, N.Y. : 1980), 2008-09, Vol.29 (9), p.1534-1543 |
| issn | 0196-9781 1873-5169 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adrenomedullin Adrenomedullin - isolation & purification Adrenomedullin-2/intermedin Amino Acid Sequence Amylin Amyloid - isolation & purification Animals Biological and medical sciences Calcitonin Gene-Related Peptide - isolation & purification Carassius auratus CGRP Cloning Cloning, Molecular Freshwater Fundamental and applied biological sciences. Psychology Goldfish Humans Islet Amyloid Polypeptide Molecular Sequence Data Neuropeptides - isolation & purification RNA, Messenger - metabolism Sequence Alignment Teleostei Tissue Distribution Vertebrates: endocrinology |
| title | Molecular characterization of calcitonin gene-related peptide (CGRP) related peptides (CGRP, amylin, adrenomedullin and adrenomedullin-2/intermedin) in goldfish ( Carassius auratus): Cloning and distribution |
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