Adsorption of salivary and serum proteins, and bacterial adherence on titanium and zirconia ceramic surfaces
: Objectives: The aim of this study was to determine the pattern of salivary and serum proteins present in pellicles formed on titanium (Ti) and zirconia ceramic (ZrO2) surfaces, and the ability of bacterial cells to adhere to the experimental pellicles. In addition, the protein profiles and bacteri...
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| Veröffentlicht in: | Clinical oral implants research 2008-08, Vol.19 (8), p.780-785 |
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| creator | Lima, Emilena Maria Castor Xisto Koo, Hyun Vacca Smith, Anne M. Rosalen, Pedro Luiz Del Bel Cury, Altair Antoninha |
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Objectives: The aim of this study was to determine the pattern of salivary and serum proteins present in pellicles formed on titanium (Ti) and zirconia ceramic (ZrO2) surfaces, and the ability of bacterial cells to adhere to the experimental pellicles. In addition, the protein profiles and bacterial binding properties of pellicles on Ti and ZrO2 were compared to those formed on hydroxyapatite (HA) surface.
Methods: The pellicles were formed in vitro by incubating the materials with whole saliva, serum or saliva+serum. Protein composition in each of the pellicles was investigated by SDS‐PAGE and immunodetection. The adherence of radiolabeled Streptococcus mutans and Actinomyces naeslundii to uncoated surfaces and experimental pellicles was determined by means of scintillation counting. Statistical analyses were done using ANOVA and Tukey's test at significance level at P |
| doi_str_mv | 10.1111/j.1600-0501.2008.01524.x |
| format | Article |
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Objectives: The aim of this study was to determine the pattern of salivary and serum proteins present in pellicles formed on titanium (Ti) and zirconia ceramic (ZrO2) surfaces, and the ability of bacterial cells to adhere to the experimental pellicles. In addition, the protein profiles and bacterial binding properties of pellicles on Ti and ZrO2 were compared to those formed on hydroxyapatite (HA) surface.
Methods: The pellicles were formed in vitro by incubating the materials with whole saliva, serum or saliva+serum. Protein composition in each of the pellicles was investigated by SDS‐PAGE and immunodetection. The adherence of radiolabeled Streptococcus mutans and Actinomyces naeslundii to uncoated surfaces and experimental pellicles was determined by means of scintillation counting. Statistical analyses were done using ANOVA and Tukey's test at significance level at P<0.05. In general, the electrophoretic analysis of the pellicles formed on HA, Ti and ZrO2 revealed few qualitative differences of the composition of proteins of the pellicles formed on HA, Ti and ZrO2 surfaces. Pellicle components identified included amylase, IgA, IgG, albumin, fibronectin and fibrinogen. The number of S. mutans cells adhered to uncoated Ti and ZrO2 was significantly higher than those adhered to HA (P<0.05). In contrast, lower number of A. naeslundii cells adhered to uncoated Ti and ZrO2 than to HA (P<0.05). However, the presence of saliva and saliva+serum pellicles greatly reduced the number of S. mutans cells bound to each of the surfaces. The data showed that Ti and ZrO2 display similar pellicle protein composition and bacterial binding properties; however, significant differences were observed when both materials were compared to HA.</description><identifier>ISSN: 0905-7161</identifier><identifier>EISSN: 1600-0501</identifier><identifier>DOI: 10.1111/j.1600-0501.2008.01524.x</identifier><identifier>PMID: 18705809</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Actinomyces ; Adsorption ; Analysis of Variance ; Bacteria ; bacterial adherence ; Bacterial Adhesion ; Biofilms ; Blood Proteins - analysis ; Blood Proteins - classification ; Colony Count, Microbial ; Dental Enamel - microbiology ; Dental Pellicle - chemistry ; Dental Pellicle - microbiology ; Dentistry ; Durapatite ; Humans ; Metal Ceramic Alloys ; salivary pellicle ; Salivary Proteins and Peptides - analysis ; Salivary Proteins and Peptides - classification ; serum proteins ; Statistics, Nonparametric ; Streptococcus mutans ; Surface Properties ; Titanium ; zirconia ; Zirconium</subject><ispartof>Clinical oral implants research, 2008-08, Vol.19 (8), p.780-785</ispartof><rights>2008 The Authors. Journal compilation © 2008 Blackwell Munksgaard</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5024-5e23d7f0a215a976f4ba6db75d2192baa1fc5168f28f7d8ff3583842a79e78153</citedby><cites>FETCH-LOGICAL-c5024-5e23d7f0a215a976f4ba6db75d2192baa1fc5168f28f7d8ff3583842a79e78153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0501.2008.01524.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0501.2008.01524.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18705809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lima, Emilena Maria Castor Xisto</creatorcontrib><creatorcontrib>Koo, Hyun</creatorcontrib><creatorcontrib>Vacca Smith, Anne M.</creatorcontrib><creatorcontrib>Rosalen, Pedro Luiz</creatorcontrib><creatorcontrib>Del Bel Cury, Altair Antoninha</creatorcontrib><title>Adsorption of salivary and serum proteins, and bacterial adherence on titanium and zirconia ceramic surfaces</title><title>Clinical oral implants research</title><addtitle>Clin Oral Implants Res</addtitle><description>:
Objectives: The aim of this study was to determine the pattern of salivary and serum proteins present in pellicles formed on titanium (Ti) and zirconia ceramic (ZrO2) surfaces, and the ability of bacterial cells to adhere to the experimental pellicles. In addition, the protein profiles and bacterial binding properties of pellicles on Ti and ZrO2 were compared to those formed on hydroxyapatite (HA) surface.
Methods: The pellicles were formed in vitro by incubating the materials with whole saliva, serum or saliva+serum. Protein composition in each of the pellicles was investigated by SDS‐PAGE and immunodetection. The adherence of radiolabeled Streptococcus mutans and Actinomyces naeslundii to uncoated surfaces and experimental pellicles was determined by means of scintillation counting. Statistical analyses were done using ANOVA and Tukey's test at significance level at P<0.05. In general, the electrophoretic analysis of the pellicles formed on HA, Ti and ZrO2 revealed few qualitative differences of the composition of proteins of the pellicles formed on HA, Ti and ZrO2 surfaces. Pellicle components identified included amylase, IgA, IgG, albumin, fibronectin and fibrinogen. The number of S. mutans cells adhered to uncoated Ti and ZrO2 was significantly higher than those adhered to HA (P<0.05). In contrast, lower number of A. naeslundii cells adhered to uncoated Ti and ZrO2 than to HA (P<0.05). However, the presence of saliva and saliva+serum pellicles greatly reduced the number of S. mutans cells bound to each of the surfaces. The data showed that Ti and ZrO2 display similar pellicle protein composition and bacterial binding properties; however, significant differences were observed when both materials were compared to HA.</description><subject>Actinomyces</subject><subject>Adsorption</subject><subject>Analysis of Variance</subject><subject>Bacteria</subject><subject>bacterial adherence</subject><subject>Bacterial Adhesion</subject><subject>Biofilms</subject><subject>Blood Proteins - analysis</subject><subject>Blood Proteins - classification</subject><subject>Colony Count, Microbial</subject><subject>Dental Enamel - microbiology</subject><subject>Dental Pellicle - chemistry</subject><subject>Dental Pellicle - microbiology</subject><subject>Dentistry</subject><subject>Durapatite</subject><subject>Humans</subject><subject>Metal Ceramic Alloys</subject><subject>salivary pellicle</subject><subject>Salivary Proteins and Peptides - analysis</subject><subject>Salivary Proteins and Peptides - classification</subject><subject>serum proteins</subject><subject>Statistics, Nonparametric</subject><subject>Streptococcus mutans</subject><subject>Surface Properties</subject><subject>Titanium</subject><subject>zirconia</subject><subject>Zirconium</subject><issn>0905-7161</issn><issn>1600-0501</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9vFCEYh4mxsWv1KxhOnpwRmGFgDh6a1a0mmzZtNB7JO8xLZJ0_K8zo1k8v093Uo3KBwPMA-f0IoZzlPI23u5xXjGVMMp4LxnTOuBRlfnhCVo8HT8mK1Uxmilf8nDyPcccYq2pdPyPnXCsmNatXpLts4xj2kx8HOjoaofM_IdxTGFoaMcw93YdxQj_ENw97DdgJg4eOQvsNAw4WaVInP8HgE70wv32w4-CBWgzQe0vjHBxYjC_ImYMu4svTfEG-bD58Xn_MtjdXn9aX28xKJspMoiha5RgILqFWlSsbqNpGyVbwWjQA3FnJK-2EdqrVzhVSF7oUoGpUmsvigrw-3pv-_mPGOJneR4tdBwOOczRVXRZKKPVPkKe4dKmKBOojaMMYY0Bn9sH3KSjDmVkqMTuzJG-W5M1SiXmoxByS-ur0xtz02P4VTx0k4N0R-OU7vP_vi816e7eskp8dfR8nPDz6EL6bShVKmq_XV0Ztbt_fblRpRPEHqWap7w</recordid><startdate>200808</startdate><enddate>200808</enddate><creator>Lima, Emilena Maria Castor Xisto</creator><creator>Koo, Hyun</creator><creator>Vacca Smith, Anne M.</creator><creator>Rosalen, Pedro Luiz</creator><creator>Del Bel Cury, Altair Antoninha</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200808</creationdate><title>Adsorption of salivary and serum proteins, and bacterial adherence on titanium and zirconia ceramic surfaces</title><author>Lima, Emilena Maria Castor Xisto ; Koo, Hyun ; Vacca Smith, Anne M. ; Rosalen, Pedro Luiz ; Del Bel Cury, Altair Antoninha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5024-5e23d7f0a215a976f4ba6db75d2192baa1fc5168f28f7d8ff3583842a79e78153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Actinomyces</topic><topic>Adsorption</topic><topic>Analysis of Variance</topic><topic>Bacteria</topic><topic>bacterial adherence</topic><topic>Bacterial Adhesion</topic><topic>Biofilms</topic><topic>Blood Proteins - analysis</topic><topic>Blood Proteins - classification</topic><topic>Colony Count, Microbial</topic><topic>Dental Enamel - microbiology</topic><topic>Dental Pellicle - chemistry</topic><topic>Dental Pellicle - microbiology</topic><topic>Dentistry</topic><topic>Durapatite</topic><topic>Humans</topic><topic>Metal Ceramic Alloys</topic><topic>salivary pellicle</topic><topic>Salivary Proteins and Peptides - analysis</topic><topic>Salivary Proteins and Peptides - classification</topic><topic>serum proteins</topic><topic>Statistics, Nonparametric</topic><topic>Streptococcus mutans</topic><topic>Surface Properties</topic><topic>Titanium</topic><topic>zirconia</topic><topic>Zirconium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lima, Emilena Maria Castor Xisto</creatorcontrib><creatorcontrib>Koo, Hyun</creatorcontrib><creatorcontrib>Vacca Smith, Anne M.</creatorcontrib><creatorcontrib>Rosalen, Pedro Luiz</creatorcontrib><creatorcontrib>Del Bel Cury, Altair Antoninha</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical oral implants research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lima, Emilena Maria Castor Xisto</au><au>Koo, Hyun</au><au>Vacca Smith, Anne M.</au><au>Rosalen, Pedro Luiz</au><au>Del Bel Cury, Altair Antoninha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adsorption of salivary and serum proteins, and bacterial adherence on titanium and zirconia ceramic surfaces</atitle><jtitle>Clinical oral implants research</jtitle><addtitle>Clin Oral Implants Res</addtitle><date>2008-08</date><risdate>2008</risdate><volume>19</volume><issue>8</issue><spage>780</spage><epage>785</epage><pages>780-785</pages><issn>0905-7161</issn><eissn>1600-0501</eissn><abstract>:
Objectives: The aim of this study was to determine the pattern of salivary and serum proteins present in pellicles formed on titanium (Ti) and zirconia ceramic (ZrO2) surfaces, and the ability of bacterial cells to adhere to the experimental pellicles. In addition, the protein profiles and bacterial binding properties of pellicles on Ti and ZrO2 were compared to those formed on hydroxyapatite (HA) surface.
Methods: The pellicles were formed in vitro by incubating the materials with whole saliva, serum or saliva+serum. Protein composition in each of the pellicles was investigated by SDS‐PAGE and immunodetection. The adherence of radiolabeled Streptococcus mutans and Actinomyces naeslundii to uncoated surfaces and experimental pellicles was determined by means of scintillation counting. Statistical analyses were done using ANOVA and Tukey's test at significance level at P<0.05. In general, the electrophoretic analysis of the pellicles formed on HA, Ti and ZrO2 revealed few qualitative differences of the composition of proteins of the pellicles formed on HA, Ti and ZrO2 surfaces. Pellicle components identified included amylase, IgA, IgG, albumin, fibronectin and fibrinogen. The number of S. mutans cells adhered to uncoated Ti and ZrO2 was significantly higher than those adhered to HA (P<0.05). In contrast, lower number of A. naeslundii cells adhered to uncoated Ti and ZrO2 than to HA (P<0.05). However, the presence of saliva and saliva+serum pellicles greatly reduced the number of S. mutans cells bound to each of the surfaces. The data showed that Ti and ZrO2 display similar pellicle protein composition and bacterial binding properties; however, significant differences were observed when both materials were compared to HA.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>18705809</pmid><doi>10.1111/j.1600-0501.2008.01524.x</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Actinomyces Adsorption Analysis of Variance Bacteria bacterial adherence Bacterial Adhesion Biofilms Blood Proteins - analysis Blood Proteins - classification Colony Count, Microbial Dental Enamel - microbiology Dental Pellicle - chemistry Dental Pellicle - microbiology Dentistry Durapatite Humans Metal Ceramic Alloys salivary pellicle Salivary Proteins and Peptides - analysis Salivary Proteins and Peptides - classification serum proteins Statistics, Nonparametric Streptococcus mutans Surface Properties Titanium zirconia Zirconium |
| title | Adsorption of salivary and serum proteins, and bacterial adherence on titanium and zirconia ceramic surfaces |
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