The Unique Neuropeptide Pattern in Abdominal Perisympathetic Organs of Insects
: We successfully isolated and identified the abundant neuropeptides of the abdominal perisympathetic organs of the American cockroach, including all myoactive compounds. Peptide sequence analysis and mass spectrometry of abundant substances that were not bioactive in different muscle assays yielded...
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| Veröffentlicht in: | Annals of the New York Academy of Sciences 1999-01, Vol.897 (1), p.282-290 |
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| container_title | Annals of the New York Academy of Sciences |
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| creator | PREDEL, REINHARD ECKERT, MANFRED HOLMAN, G. MARK |
| description | : We successfully isolated and identified the abundant neuropeptides of the abdominal perisympathetic organs of the American cockroach, including all myoactive compounds. Peptide sequence analysis and mass spectrometry of abundant substances that were not bioactive in different muscle assays yielded the following sequences: TDPLWQLPGAHLEQYLS‐NH2 (Pea‐YLSamide), AFLTLTPGSHVDSYVEA‐OH (Pea‐VEAacid), and SDLTW‐TYQSPGDPTNSKN‐OH (Pea‐SKNacid). The given structures led to the conclusion of an unique neuropeptide pattern in abdominal perisympathetic organs. We confirmed this assumption with immunocytochemical studies, using antisera raised against different myotropic neuropeptides of the abdominal perisympathetic organs. Moreover, mass spectrometric methods, developed for the investigation of single neurohemal organs, confirmed the neuropeptide pattern in these organs. |
| doi_str_mv | 10.1111/j.1749-6632.1999.tb07899.x |
| format | Article |
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MARK</creatorcontrib><title>The Unique Neuropeptide Pattern in Abdominal Perisympathetic Organs of Insects</title><title>Annals of the New York Academy of Sciences</title><addtitle>Ann N Y Acad Sci</addtitle><description>: We successfully isolated and identified the abundant neuropeptides of the abdominal perisympathetic organs of the American cockroach, including all myoactive compounds. Peptide sequence analysis and mass spectrometry of abundant substances that were not bioactive in different muscle assays yielded the following sequences: TDPLWQLPGAHLEQYLS‐NH2 (Pea‐YLSamide), AFLTLTPGSHVDSYVEA‐OH (Pea‐VEAacid), and SDLTW‐TYQSPGDPTNSKN‐OH (Pea‐SKNacid). The given structures led to the conclusion of an unique neuropeptide pattern in abdominal perisympathetic organs. We confirmed this assumption with immunocytochemical studies, using antisera raised against different myotropic neuropeptides of the abdominal perisympathetic organs. Moreover, mass spectrometric methods, developed for the investigation of single neurohemal organs, confirmed the neuropeptide pattern in these organs.</description><subject>Abdomen</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological Assay</subject><subject>Cockroaches</subject><subject>Ganglia, Invertebrate - chemistry</subject><subject>Ganglia, Invertebrate - cytology</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Muscles - drug effects</subject><subject>Neuropeptides - analysis</subject><subject>Neuropeptides - chemistry</subject><subject>Neuropeptides - pharmacology</subject><subject>Neurosecretory Systems - chemistry</subject><subject>Neurosecretory Systems - cytology</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><issn>0077-8923</issn><issn>1749-6632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkE1rGzEQhkVJqN0kf6GIHnJbRx-70qqXYkLsGIzjUoeQk9DuztZy9yuSTO1_3zVrQq6dyxzmnWeGB6FvlExoX3e7CZWxioTgbEKVUpOQEZn2_fAJjd9HF2hMiJRRqhgfoS_e7wihLI3lZzSiREgRJ2KMVpst4OfGvu0Br2Dv2g66YAvAaxMCuAbbBk-zoq1tYyq8Bmf9se5M2EKwOX5yv03jcVviReMhD_4aXZam8nBz7lfoefawuX-Mlk_zxf10GeUxUSyKi4yQtIDcmFIkqsyUYoUopBIxExktlQIgJUsKRTNIOZFlYoyQLE8EV8AFv0K3A7dzbf-6D7q2PoeqMg20e6-FijmlhPfB70Mwd633DkrdOVsbd9SU6JNNvdMnZfqkTJ9s6rNNfeiXv56v7LMaig-rg74-8GMI_LUVHP8DrVev018sZT0hGgjWBzi8E4z7o4XkMtEvq7lek5marTdL_ZP_AzLalSM</recordid><startdate>19990101</startdate><enddate>19990101</enddate><creator>PREDEL, REINHARD</creator><creator>ECKERT, MANFRED</creator><creator>HOLMAN, G. 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MARK</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4092-4db008decaaf659fb992d6d796426b1f99ee0f25d91be8307f5aa672c5639e363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Abdomen</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological Assay</topic><topic>Cockroaches</topic><topic>Ganglia, Invertebrate - chemistry</topic><topic>Ganglia, Invertebrate - cytology</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Muscles - drug effects</topic><topic>Neuropeptides - analysis</topic><topic>Neuropeptides - chemistry</topic><topic>Neuropeptides - pharmacology</topic><topic>Neurosecretory Systems - chemistry</topic><topic>Neurosecretory Systems - cytology</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PREDEL, REINHARD</creatorcontrib><creatorcontrib>ECKERT, MANFRED</creatorcontrib><creatorcontrib>HOLMAN, G. 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MARK</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Unique Neuropeptide Pattern in Abdominal Perisympathetic Organs of Insects</atitle><jtitle>Annals of the New York Academy of Sciences</jtitle><addtitle>Ann N Y Acad Sci</addtitle><date>1999-01-01</date><risdate>1999</risdate><volume>897</volume><issue>1</issue><spage>282</spage><epage>290</epage><pages>282-290</pages><issn>0077-8923</issn><eissn>1749-6632</eissn><abstract>: We successfully isolated and identified the abundant neuropeptides of the abdominal perisympathetic organs of the American cockroach, including all myoactive compounds. Peptide sequence analysis and mass spectrometry of abundant substances that were not bioactive in different muscle assays yielded the following sequences: TDPLWQLPGAHLEQYLS‐NH2 (Pea‐YLSamide), AFLTLTPGSHVDSYVEA‐OH (Pea‐VEAacid), and SDLTW‐TYQSPGDPTNSKN‐OH (Pea‐SKNacid). The given structures led to the conclusion of an unique neuropeptide pattern in abdominal perisympathetic organs. We confirmed this assumption with immunocytochemical studies, using antisera raised against different myotropic neuropeptides of the abdominal perisympathetic organs. Moreover, mass spectrometric methods, developed for the investigation of single neurohemal organs, confirmed the neuropeptide pattern in these organs.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10676456</pmid><doi>10.1111/j.1749-6632.1999.tb07899.x</doi><tpages>9</tpages></addata></record> |
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| ispartof | Annals of the New York Academy of Sciences, 1999-01, Vol.897 (1), p.282-290 |
| issn | 0077-8923 1749-6632 |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Abdomen Amino Acid Sequence Animals Biological Assay Cockroaches Ganglia, Invertebrate - chemistry Ganglia, Invertebrate - cytology Immunohistochemistry Male Molecular Sequence Data Muscles - drug effects Neuropeptides - analysis Neuropeptides - chemistry Neuropeptides - pharmacology Neurosecretory Systems - chemistry Neurosecretory Systems - cytology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title | The Unique Neuropeptide Pattern in Abdominal Perisympathetic Organs of Insects |
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