Molecular mass distribution of sodium alginate by high-performance size-exclusion chromatography
A sensitive high-performance size-exclusion chromatography (HPSEC) method with simple UV detection was developed for the molecular mass analysis of sodium alginate. It was used to evaluate alginates of varying molecular mass and the results were compared with the viscosity measurements. This HPSEC m...
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| Veröffentlicht in: | Journal of Chromatography A 1999-12, Vol.864 (2), p.199-210 |
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| container_end_page | 210 |
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| container_issue | 2 |
| container_start_page | 199 |
| container_title | Journal of Chromatography A |
| container_volume | 864 |
| creator | Ci, Sherry X Huynh, Tanya H Louie, Leslie W Yang, Andrew Beals, Bridget J Ron, Nilesh Tsang, Wen-Ghih Soon-Shiong, Patrick Desai, Neil P |
| description | A sensitive high-performance size-exclusion chromatography (HPSEC) method with simple UV detection was developed for the molecular mass analysis of sodium alginate. It was used to evaluate alginates of varying molecular mass and the results were compared with the viscosity measurements. This HPSEC method was sensitive to serve as the stability indicating method for alginate after storage under different conditions. The information of relative molecular mass distribution of alginate was provided with reference to pullulan molecular mass standards. The comparison of the HPSEC chromatograms of alginate, pullulan and dextran revealed the effect of chemical composition of a polysaccharide and its effect on apparent molecular mass distribution. |
| doi_str_mv | 10.1016/S0021-9673(99)01029-8 |
| format | Article |
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It was used to evaluate alginates of varying molecular mass and the results were compared with the viscosity measurements. This HPSEC method was sensitive to serve as the stability indicating method for alginate after storage under different conditions. The information of relative molecular mass distribution of alginate was provided with reference to pullulan molecular mass standards. 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The comparison of the HPSEC chromatograms of alginate, pullulan and dextran revealed the effect of chemical composition of a polysaccharide and its effect on apparent molecular mass distribution.</description><subject>Alginates - chemistry</subject><subject>Applied sciences</subject><subject>Chromatography, Gel</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dextran</subject><subject>Exact sciences and technology</subject><subject>Glucuronic Acid</subject><subject>Hexuronic Acids</subject><subject>Molecular Weight</subject><subject>Natural polymers</subject><subject>Physicochemistry of polymers</subject><subject>Polysaccharides</subject><subject>Pullulan</subject><subject>Quality Control</subject><subject>Sodium alginate</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Starch and polysaccharides</subject><subject>Viscosity</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAQhn2g4rM_AZRDheAQOs6HkzkhhKCtBOoBejaOPdl1lay3ngSx_Hqy7Kr01tNIo-edj0eIYwkXEqT6-gCQyRRVlZ8hnoOEDNN6R-z_be-JA-bfALKCKtsVexKUwqyu9sXTfejIjp2JSW-YE-d5iL4ZBx8WSWgTDs6PfWK6mV-YgZJmlcz9bJ4uKbYh9mZhKWH_Sim92G7kdcrOY-jNEGbRLOerI_GpNR3T5209FL9ubx6vv6d3P7_9uL66S22OMKQluMJgi3njEKBqEbKsymtXWldIJWuFlaumX4lQkWysLdq6kbmjuikLRMwPxelm7jKGPyPxoHvPlrrOLCiMrBUWWV2gnMByA9oYmCO1ehl9b-JKS9Brnfpdp15704j6Xaeup9zJdsHY9OT-SW1cTsCXLWDYmq6NkxzPH1ymyhzWcy43GE02nj1FzdbT5NH5SHbQLvj_XPIGVsKU-g</recordid><startdate>19991224</startdate><enddate>19991224</enddate><creator>Ci, Sherry X</creator><creator>Huynh, Tanya H</creator><creator>Louie, Leslie W</creator><creator>Yang, Andrew</creator><creator>Beals, Bridget J</creator><creator>Ron, Nilesh</creator><creator>Tsang, Wen-Ghih</creator><creator>Soon-Shiong, Patrick</creator><creator>Desai, Neil P</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991224</creationdate><title>Molecular mass distribution of sodium alginate by high-performance size-exclusion chromatography</title><author>Ci, Sherry X ; Huynh, Tanya H ; Louie, Leslie W ; Yang, Andrew ; Beals, Bridget J ; Ron, Nilesh ; Tsang, Wen-Ghih ; Soon-Shiong, Patrick ; Desai, Neil P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-50d4a9f93bd9007f9022738d5cd41618697d7016ee96e1bcc4f8b13de8b549993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Alginates - chemistry</topic><topic>Applied sciences</topic><topic>Chromatography, Gel</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dextran</topic><topic>Exact sciences and technology</topic><topic>Glucuronic Acid</topic><topic>Hexuronic Acids</topic><topic>Molecular Weight</topic><topic>Natural polymers</topic><topic>Physicochemistry of polymers</topic><topic>Polysaccharides</topic><topic>Pullulan</topic><topic>Quality Control</topic><topic>Sodium alginate</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Starch and polysaccharides</topic><topic>Viscosity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ci, Sherry X</creatorcontrib><creatorcontrib>Huynh, Tanya H</creatorcontrib><creatorcontrib>Louie, Leslie W</creatorcontrib><creatorcontrib>Yang, Andrew</creatorcontrib><creatorcontrib>Beals, Bridget J</creatorcontrib><creatorcontrib>Ron, Nilesh</creatorcontrib><creatorcontrib>Tsang, Wen-Ghih</creatorcontrib><creatorcontrib>Soon-Shiong, Patrick</creatorcontrib><creatorcontrib>Desai, Neil P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ci, Sherry X</au><au>Huynh, Tanya H</au><au>Louie, Leslie W</au><au>Yang, Andrew</au><au>Beals, Bridget J</au><au>Ron, Nilesh</au><au>Tsang, Wen-Ghih</au><au>Soon-Shiong, Patrick</au><au>Desai, Neil P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular mass distribution of sodium alginate by high-performance size-exclusion chromatography</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>1999-12-24</date><risdate>1999</risdate><volume>864</volume><issue>2</issue><spage>199</spage><epage>210</epage><pages>199-210</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>A sensitive high-performance size-exclusion chromatography (HPSEC) method with simple UV detection was developed for the molecular mass analysis of sodium alginate. 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| fulltext | fulltext |
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| ispartof | Journal of Chromatography A, 1999-12, Vol.864 (2), p.199-210 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Alginates - chemistry Applied sciences Chromatography, Gel Chromatography, High Pressure Liquid Dextran Exact sciences and technology Glucuronic Acid Hexuronic Acids Molecular Weight Natural polymers Physicochemistry of polymers Polysaccharides Pullulan Quality Control Sodium alginate Spectrophotometry, Ultraviolet Starch and polysaccharides Viscosity |
| title | Molecular mass distribution of sodium alginate by high-performance size-exclusion chromatography |
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