The microbial ecology of a high-temperature near-neutral spring situated in Rotorua, New Zealand
A combination of both culture and culture-independent techniques were used to investigate the microbial ecology of a near-neutral, high-temperature hot spring (designated AQ1) in Rotorua, New Zealand. The active microbial members of the community were targeted by analyzing biofilms that developed on...
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| Veröffentlicht in: | Microbiological research 2008-01, Vol.163 (5), p.594-603 |
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| creator | Niederberger, Thomas D. Ronimus, Ron S. Morgan, Hugh W. |
| description | A combination of both culture and culture-independent techniques were used to investigate the microbial ecology of a near-neutral, high-temperature hot spring (designated AQ1) in Rotorua, New Zealand. The active microbial members of the community were targeted by analyzing biofilms that developed on surfaces incubated
in situ in AQ1. Colonization of surfaces was rapid as indicated by ATP assay and microscopic observation. DNA-based analysis of both colonized surfaces and pool water from AQ1 revealed an exclusively archaeal community. Different colonization patterns were observed on glass slides incubated near the pool surface or at depth. Slides incubated at the surface were colonized exclusively by
Pyrobaculum species, while at greater depth a novel coccus was also observed and detected by DGGE. Sequence analysis revealed the coccus was related to
Aeropyrum pernix. Two microorganisms were isolated from AQ1 pool water, namely
Ignisphaera aggregans AQ1.S1
T and a species of
Pyrobaculum, isolate AQ1.S2. |
| doi_str_mv | 10.1016/j.micres.2006.09.001 |
| format | Article |
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in situ in AQ1. Colonization of surfaces was rapid as indicated by ATP assay and microscopic observation. DNA-based analysis of both colonized surfaces and pool water from AQ1 revealed an exclusively archaeal community. Different colonization patterns were observed on glass slides incubated near the pool surface or at depth. Slides incubated at the surface were colonized exclusively by
Pyrobaculum species, while at greater depth a novel coccus was also observed and detected by DGGE. Sequence analysis revealed the coccus was related to
Aeropyrum pernix. Two microorganisms were isolated from AQ1 pool water, namely
Ignisphaera aggregans AQ1.S1
T and a species of
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in situ in AQ1. Colonization of surfaces was rapid as indicated by ATP assay and microscopic observation. DNA-based analysis of both colonized surfaces and pool water from AQ1 revealed an exclusively archaeal community. Different colonization patterns were observed on glass slides incubated near the pool surface or at depth. Slides incubated at the surface were colonized exclusively by
Pyrobaculum species, while at greater depth a novel coccus was also observed and detected by DGGE. Sequence analysis revealed the coccus was related to
Aeropyrum pernix. Two microorganisms were isolated from AQ1 pool water, namely
Ignisphaera aggregans AQ1.S1
T and a species of
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The active microbial members of the community were targeted by analyzing biofilms that developed on surfaces incubated
in situ in AQ1. Colonization of surfaces was rapid as indicated by ATP assay and microscopic observation. DNA-based analysis of both colonized surfaces and pool water from AQ1 revealed an exclusively archaeal community. Different colonization patterns were observed on glass slides incubated near the pool surface or at depth. Slides incubated at the surface were colonized exclusively by
Pyrobaculum species, while at greater depth a novel coccus was also observed and detected by DGGE. Sequence analysis revealed the coccus was related to
Aeropyrum pernix. Two microorganisms were isolated from AQ1 pool water, namely
Ignisphaera aggregans AQ1.S1
T and a species of
Pyrobaculum, isolate AQ1.S2.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>17207984</pmid><doi>10.1016/j.micres.2006.09.001</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Microbiological research, 2008-01, Vol.163 (5), p.594-603 |
| issn | 0944-5013 1618-0623 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Adenosine Triphosphate - metabolism Aeropyrum pernix Archaea - classification Archaea - genetics Archaea - isolation & purification Archaea - metabolism Biofilms - growth & development DGGE Ecology Freshwater Hot spring Hot Springs - microbiology Microbial colonization Molecular Sequence Data New Zealand Phylogeny Pyrobaculum Water Microbiology |
| title | The microbial ecology of a high-temperature near-neutral spring situated in Rotorua, New Zealand |
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