A new peroxidase from garlic (Allium sativum) bulb: its use in H2O2 biosensing
Peroxidase POX1 isoenzyme was purified from garlic (Allium sativum L.) bulb by ammonium sulfate precipitation, gel filtration and anion‐exchange chromatography. Native‐PAGE profile showed two isoforms, designated POX1A and POX1B. POX1B seems to be more attractive for biosensor design since its Km (a...
Gespeichert in:
| Veröffentlicht in: | Biotechnology and applied biochemistry 2008-09, Vol.51 (1), p.33-41 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 41 |
|---|---|
| container_issue | 1 |
| container_start_page | 33 |
| container_title | Biotechnology and applied biochemistry |
| container_volume | 51 |
| creator | El Ichi, Sarra Abdelghani, Adnane Hadji, Imene Helali, Salwa Limam, Ferid Marzouki, M. Nejib |
| description | Peroxidase POX1 isoenzyme was purified from garlic (Allium sativum L.) bulb by ammonium sulfate precipitation, gel filtration and anion‐exchange chromatography. Native‐PAGE profile showed two isoforms, designated POX1A and POX1B. POX1B seems to be more attractive for biosensor design since its Km (app) for H2O2 is lower than that of POX1A. In addition to its storage and operational stability, POX1B was found to be highly heat‐stable, since almost 70% of its activity was conserved at 60 °C, whereas full activity was retained at 50 and 40 °C for 40 min. The optimal pH was approx. 5 and the optimal temperature was 30 °C. Next, gelatin was used as a matrix for enzyme immobilization on a gold electrode surface and electrochemical measurements were performed by using cyclic voltammetry. POX1B‐based electrodes show great potential for application in H2O2 monitoring of biological samples. |
| doi_str_mv | 10.1042/BA20070141 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_69419095</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69419095</sourcerecordid><originalsourceid>FETCH-LOGICAL-i2460-1b59886fb97d1f0feb334301c278201d8cf72ef1f8465ac46e289d3691c6a9bc3</originalsourceid><addsrcrecordid>eNpF0U1v1DAQBmALUdGlcOEHIF-o4JAyYzv-4LZb0S5S1SIEgpvlOHZlcJIl3vTj3xO02_Y0c3je0WiGkDcIJwiCfVwtGYACFPiMLFAoqLQS4jlZgNZ1JWrkh-RlKb8BQCvNXpBD1EwaLsyCXC5pH27pJozDXWpdCTSOQ0ev3ZiTp--XOaepo8Vt083UfaDNlJtPNG0LnWaaerpmV4w2aSihL6m_fkUOosslvN7XI_Lj7PP303V1cXX-5XR5USUmJFTY1EZrGRujWowQQ8O54ICezfsBttpHxULEqIWsnRcyMG1aLg166Uzj-RE53s3djMPfKZSt7VLxIWfXh2EqVhqBBkw9w7d7ODVdaO1mTJ0b7-3DBWbwbg9c8S7H0fU-lUfHQCIqIWd3snO3KYf7pzlg___APv1gbleKwxyodoFUtuHuMeDGP1Yqrmr78_Lcrr79OlsjfrUr_g_kw4PR</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69419095</pqid></control><display><type>article</type><title>A new peroxidase from garlic (Allium sativum) bulb: its use in H2O2 biosensing</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>El Ichi, Sarra ; Abdelghani, Adnane ; Hadji, Imene ; Helali, Salwa ; Limam, Ferid ; Marzouki, M. Nejib</creator><creatorcontrib>El Ichi, Sarra ; Abdelghani, Adnane ; Hadji, Imene ; Helali, Salwa ; Limam, Ferid ; Marzouki, M. Nejib</creatorcontrib><description>Peroxidase POX1 isoenzyme was purified from garlic (Allium sativum L.) bulb by ammonium sulfate precipitation, gel filtration and anion‐exchange chromatography. Native‐PAGE profile showed two isoforms, designated POX1A and POX1B. POX1B seems to be more attractive for biosensor design since its Km (app) for H2O2 is lower than that of POX1A. In addition to its storage and operational stability, POX1B was found to be highly heat‐stable, since almost 70% of its activity was conserved at 60 °C, whereas full activity was retained at 50 and 40 °C for 40 min. The optimal pH was approx. 5 and the optimal temperature was 30 °C. Next, gelatin was used as a matrix for enzyme immobilization on a gold electrode surface and electrochemical measurements were performed by using cyclic voltammetry. POX1B‐based electrodes show great potential for application in H2O2 monitoring of biological samples.</description><identifier>ISSN: 0885-4513</identifier><identifier>EISSN: 1470-8744</identifier><identifier>DOI: 10.1042/BA20070141</identifier><identifier>PMID: 18269349</identifier><identifier>CODEN: BABIEC</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Ammonium Sulfate - chemistry ; amperometric measurement ; Biological and medical sciences ; Biosensing Techniques - methods ; biosensor ; Biosensors ; Biotechnology ; Chemical Precipitation ; Chromatography, Gel - methods ; Chromatography, Ion Exchange - methods ; Electrochemistry ; Electrodes ; Enzyme Stability ; Enzymes, Immobilized ; Fundamental and applied biological sciences. Psychology ; garlic (Allium sativum) peroxidase ; Garlic - enzymology ; Gelatin - pharmacology ; gelatin matrix ; Gold ; gold electrode ; hydrogen peroxide (H2O2) ; Hydrogen Peroxide - analysis ; Hydrogen-Ion Concentration ; Kinetics ; Methods. Procedures. Technologies ; Peroxidase - chemistry ; Peroxidase - isolation & purification ; Peroxidase - metabolism ; Protein Isoforms - chemistry ; Protein Isoforms - isolation & purification ; Protein Isoforms - metabolism ; Temperature ; Various methods and equipments</subject><ispartof>Biotechnology and applied biochemistry, 2008-09, Vol.51 (1), p.33-41</ispartof><rights>2008 International Union of Biochemistry and Molecular Biology</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1042%2FBA20070141$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1042%2FBA20070141$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20611746$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18269349$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El Ichi, Sarra</creatorcontrib><creatorcontrib>Abdelghani, Adnane</creatorcontrib><creatorcontrib>Hadji, Imene</creatorcontrib><creatorcontrib>Helali, Salwa</creatorcontrib><creatorcontrib>Limam, Ferid</creatorcontrib><creatorcontrib>Marzouki, M. Nejib</creatorcontrib><title>A new peroxidase from garlic (Allium sativum) bulb: its use in H2O2 biosensing</title><title>Biotechnology and applied biochemistry</title><addtitle>Biotechnol Appl Biochem</addtitle><description>Peroxidase POX1 isoenzyme was purified from garlic (Allium sativum L.) bulb by ammonium sulfate precipitation, gel filtration and anion‐exchange chromatography. Native‐PAGE profile showed two isoforms, designated POX1A and POX1B. POX1B seems to be more attractive for biosensor design since its Km (app) for H2O2 is lower than that of POX1A. In addition to its storage and operational stability, POX1B was found to be highly heat‐stable, since almost 70% of its activity was conserved at 60 °C, whereas full activity was retained at 50 and 40 °C for 40 min. The optimal pH was approx. 5 and the optimal temperature was 30 °C. Next, gelatin was used as a matrix for enzyme immobilization on a gold electrode surface and electrochemical measurements were performed by using cyclic voltammetry. POX1B‐based electrodes show great potential for application in H2O2 monitoring of biological samples.</description><subject>Ammonium Sulfate - chemistry</subject><subject>amperometric measurement</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>biosensor</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemical Precipitation</subject><subject>Chromatography, Gel - methods</subject><subject>Chromatography, Ion Exchange - methods</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Enzyme Stability</subject><subject>Enzymes, Immobilized</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>garlic (Allium sativum) peroxidase</subject><subject>Garlic - enzymology</subject><subject>Gelatin - pharmacology</subject><subject>gelatin matrix</subject><subject>Gold</subject><subject>gold electrode</subject><subject>hydrogen peroxide (H2O2)</subject><subject>Hydrogen Peroxide - analysis</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kinetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Peroxidase - chemistry</subject><subject>Peroxidase - isolation & purification</subject><subject>Peroxidase - metabolism</subject><subject>Protein Isoforms - chemistry</subject><subject>Protein Isoforms - isolation & purification</subject><subject>Protein Isoforms - metabolism</subject><subject>Temperature</subject><subject>Various methods and equipments</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0U1v1DAQBmALUdGlcOEHIF-o4JAyYzv-4LZb0S5S1SIEgpvlOHZlcJIl3vTj3xO02_Y0c3je0WiGkDcIJwiCfVwtGYACFPiMLFAoqLQS4jlZgNZ1JWrkh-RlKb8BQCvNXpBD1EwaLsyCXC5pH27pJozDXWpdCTSOQ0ev3ZiTp--XOaepo8Vt083UfaDNlJtPNG0LnWaaerpmV4w2aSihL6m_fkUOosslvN7XI_Lj7PP303V1cXX-5XR5USUmJFTY1EZrGRujWowQQ8O54ICezfsBttpHxULEqIWsnRcyMG1aLg166Uzj-RE53s3djMPfKZSt7VLxIWfXh2EqVhqBBkw9w7d7ODVdaO1mTJ0b7-3DBWbwbg9c8S7H0fU-lUfHQCIqIWd3snO3KYf7pzlg___APv1gbleKwxyodoFUtuHuMeDGP1Yqrmr78_Lcrr79OlsjfrUr_g_kw4PR</recordid><startdate>200809</startdate><enddate>200809</enddate><creator>El Ichi, Sarra</creator><creator>Abdelghani, Adnane</creator><creator>Hadji, Imene</creator><creator>Helali, Salwa</creator><creator>Limam, Ferid</creator><creator>Marzouki, M. Nejib</creator><general>Blackwell Publishing Ltd</general><general>Portland Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200809</creationdate><title>A new peroxidase from garlic (Allium sativum) bulb: its use in H2O2 biosensing</title><author>El Ichi, Sarra ; Abdelghani, Adnane ; Hadji, Imene ; Helali, Salwa ; Limam, Ferid ; Marzouki, M. Nejib</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i2460-1b59886fb97d1f0feb334301c278201d8cf72ef1f8465ac46e289d3691c6a9bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Ammonium Sulfate - chemistry</topic><topic>amperometric measurement</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - methods</topic><topic>biosensor</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chemical Precipitation</topic><topic>Chromatography, Gel - methods</topic><topic>Chromatography, Ion Exchange - methods</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Enzyme Stability</topic><topic>Enzymes, Immobilized</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>garlic (Allium sativum) peroxidase</topic><topic>Garlic - enzymology</topic><topic>Gelatin - pharmacology</topic><topic>gelatin matrix</topic><topic>Gold</topic><topic>gold electrode</topic><topic>hydrogen peroxide (H2O2)</topic><topic>Hydrogen Peroxide - analysis</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kinetics</topic><topic>Methods. Procedures. Technologies</topic><topic>Peroxidase - chemistry</topic><topic>Peroxidase - isolation & purification</topic><topic>Peroxidase - metabolism</topic><topic>Protein Isoforms - chemistry</topic><topic>Protein Isoforms - isolation & purification</topic><topic>Protein Isoforms - metabolism</topic><topic>Temperature</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El Ichi, Sarra</creatorcontrib><creatorcontrib>Abdelghani, Adnane</creatorcontrib><creatorcontrib>Hadji, Imene</creatorcontrib><creatorcontrib>Helali, Salwa</creatorcontrib><creatorcontrib>Limam, Ferid</creatorcontrib><creatorcontrib>Marzouki, M. Nejib</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and applied biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El Ichi, Sarra</au><au>Abdelghani, Adnane</au><au>Hadji, Imene</au><au>Helali, Salwa</au><au>Limam, Ferid</au><au>Marzouki, M. Nejib</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new peroxidase from garlic (Allium sativum) bulb: its use in H2O2 biosensing</atitle><jtitle>Biotechnology and applied biochemistry</jtitle><addtitle>Biotechnol Appl Biochem</addtitle><date>2008-09</date><risdate>2008</risdate><volume>51</volume><issue>1</issue><spage>33</spage><epage>41</epage><pages>33-41</pages><issn>0885-4513</issn><eissn>1470-8744</eissn><coden>BABIEC</coden><abstract>Peroxidase POX1 isoenzyme was purified from garlic (Allium sativum L.) bulb by ammonium sulfate precipitation, gel filtration and anion‐exchange chromatography. Native‐PAGE profile showed two isoforms, designated POX1A and POX1B. POX1B seems to be more attractive for biosensor design since its Km (app) for H2O2 is lower than that of POX1A. In addition to its storage and operational stability, POX1B was found to be highly heat‐stable, since almost 70% of its activity was conserved at 60 °C, whereas full activity was retained at 50 and 40 °C for 40 min. The optimal pH was approx. 5 and the optimal temperature was 30 °C. Next, gelatin was used as a matrix for enzyme immobilization on a gold electrode surface and electrochemical measurements were performed by using cyclic voltammetry. POX1B‐based electrodes show great potential for application in H2O2 monitoring of biological samples.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>18269349</pmid><doi>10.1042/BA20070141</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0885-4513 |
| ispartof | Biotechnology and applied biochemistry, 2008-09, Vol.51 (1), p.33-41 |
| issn | 0885-4513 1470-8744 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69419095 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Ammonium Sulfate - chemistry amperometric measurement Biological and medical sciences Biosensing Techniques - methods biosensor Biosensors Biotechnology Chemical Precipitation Chromatography, Gel - methods Chromatography, Ion Exchange - methods Electrochemistry Electrodes Enzyme Stability Enzymes, Immobilized Fundamental and applied biological sciences. Psychology garlic (Allium sativum) peroxidase Garlic - enzymology Gelatin - pharmacology gelatin matrix Gold gold electrode hydrogen peroxide (H2O2) Hydrogen Peroxide - analysis Hydrogen-Ion Concentration Kinetics Methods. Procedures. Technologies Peroxidase - chemistry Peroxidase - isolation & purification Peroxidase - metabolism Protein Isoforms - chemistry Protein Isoforms - isolation & purification Protein Isoforms - metabolism Temperature Various methods and equipments |
| title | A new peroxidase from garlic (Allium sativum) bulb: its use in H2O2 biosensing |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T05%3A10%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20new%20peroxidase%20from%20garlic%20(Allium%20sativum)%20bulb:%20its%20use%20in%20H2O2%20biosensing&rft.jtitle=Biotechnology%20and%20applied%20biochemistry&rft.au=El%20Ichi,%20Sarra&rft.date=2008-09&rft.volume=51&rft.issue=1&rft.spage=33&rft.epage=41&rft.pages=33-41&rft.issn=0885-4513&rft.eissn=1470-8744&rft.coden=BABIEC&rft_id=info:doi/10.1042/BA20070141&rft_dat=%3Cproquest_pubme%3E69419095%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69419095&rft_id=info:pmid/18269349&rfr_iscdi=true |