Control of Muscle Cell-Type Specification in the Zebrafish Embryo by Hedgehog Signalling
The specification of different muscle cell types in the zebrafish embryo requires signals that emanate from the axial mesoderm. In previous studies we and others have shown that overexpression of different members of the Hedgehog protein family can induce the differentiation of two types of slow-twi...
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| Veröffentlicht in: | Developmental biology 1999-12, Vol.216 (2), p.469-480 |
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| creator | Lewis, K.E Currie, P.D Roy, S Schauerte, H Haffter, P Ingham, P.W |
| description | The specification of different muscle cell types in the zebrafish embryo requires signals that emanate from the axial mesoderm. In previous studies we and others have shown that overexpression of different members of the Hedgehog protein family can induce the differentiation of two types of slow-twitch muscles, the superficially located slow-twitch fibres and the medially located muscle pioneer cells. Here we have investigated the requirement for Hedgehog signalling in the specification of these distinct muscle cell types in two ways: first, by characterising the effects on target gene expression and muscle cell differentiation of the u-type mutants, members of a phenotypic group previously implicated in Hedgehog signalling, and second, by analysing the effects of overexpression of the Patched1 protein, a negative regulator of Hedgehog signalling. Our results support the idea that most u-type genes are required for Hedgehog signalling and indicate that while such signalling is essential for slow myocyte differentiation, the loss of activity of one signal, Sonic hedgehog, can be partially compensated for by other Hedgehog family proteins. |
| doi_str_mv | 10.1006/dbio.1999.9519 |
| format | Article |
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In previous studies we and others have shown that overexpression of different members of the Hedgehog protein family can induce the differentiation of two types of slow-twitch muscles, the superficially located slow-twitch fibres and the medially located muscle pioneer cells. Here we have investigated the requirement for Hedgehog signalling in the specification of these distinct muscle cell types in two ways: first, by characterising the effects on target gene expression and muscle cell differentiation of the u-type mutants, members of a phenotypic group previously implicated in Hedgehog signalling, and second, by analysing the effects of overexpression of the Patched1 protein, a negative regulator of Hedgehog signalling. Our results support the idea that most u-type genes are required for Hedgehog signalling and indicate that while such signalling is essential for slow myocyte differentiation, the loss of activity of one signal, Sonic hedgehog, can be partially compensated for by other Hedgehog family proteins.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1006/dbio.1999.9519</identifier><identifier>PMID: 10642786</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Differentiation ; chameleon ; Fluorescent Antibody Technique ; Gene Expression Regulation, Developmental ; Gli2 ; Hedgehog Proteins ; In Situ Hybridization ; Kruppel-Like Transcription Factors ; Membrane Proteins - genetics ; muscle fibre type ; Muscles - embryology ; Muscles - metabolism ; Mutation ; myoD ; MyoD Protein - genetics ; myosin heavy chain ; Myosins - metabolism ; Patched Receptors ; Patched-1 Receptor ; Patched1 ; Proteins - metabolism ; Receptors, Cell Surface ; RNA, Messenger - metabolism ; Signal Transduction ; Somites - metabolism ; Sonic hedgehog ; sonic you ; Trans-Activators ; Transcription Factors - metabolism ; u-boot ; u-type mutants ; you ; you-too ; Zebrafish ; Zebrafish Proteins ; Zinc Finger Protein Gli2</subject><ispartof>Developmental biology, 1999-12, Vol.216 (2), p.469-480</ispartof><rights>1999 Academic Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-8b4311db2329e4d29c5eb1b4b7e796fc8ef1949365cb9c7b14f5f193141f06483</citedby><cites>FETCH-LOGICAL-c380t-8b4311db2329e4d29c5eb1b4b7e796fc8ef1949365cb9c7b14f5f193141f06483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/dbio.1999.9519$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10642786$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lewis, K.E</creatorcontrib><creatorcontrib>Currie, P.D</creatorcontrib><creatorcontrib>Roy, S</creatorcontrib><creatorcontrib>Schauerte, H</creatorcontrib><creatorcontrib>Haffter, P</creatorcontrib><creatorcontrib>Ingham, P.W</creatorcontrib><title>Control of Muscle Cell-Type Specification in the Zebrafish Embryo by Hedgehog Signalling</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>The specification of different muscle cell types in the zebrafish embryo requires signals that emanate from the axial mesoderm. In previous studies we and others have shown that overexpression of different members of the Hedgehog protein family can induce the differentiation of two types of slow-twitch muscles, the superficially located slow-twitch fibres and the medially located muscle pioneer cells. Here we have investigated the requirement for Hedgehog signalling in the specification of these distinct muscle cell types in two ways: first, by characterising the effects on target gene expression and muscle cell differentiation of the u-type mutants, members of a phenotypic group previously implicated in Hedgehog signalling, and second, by analysing the effects of overexpression of the Patched1 protein, a negative regulator of Hedgehog signalling. Our results support the idea that most u-type genes are required for Hedgehog signalling and indicate that while such signalling is essential for slow myocyte differentiation, the loss of activity of one signal, Sonic hedgehog, can be partially compensated for by other Hedgehog family proteins.</description><subject>Animals</subject><subject>Cell Differentiation</subject><subject>chameleon</subject><subject>Fluorescent Antibody Technique</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gli2</subject><subject>Hedgehog Proteins</subject><subject>In Situ Hybridization</subject><subject>Kruppel-Like Transcription Factors</subject><subject>Membrane Proteins - genetics</subject><subject>muscle fibre type</subject><subject>Muscles - embryology</subject><subject>Muscles - metabolism</subject><subject>Mutation</subject><subject>myoD</subject><subject>MyoD Protein - genetics</subject><subject>myosin heavy chain</subject><subject>Myosins - metabolism</subject><subject>Patched Receptors</subject><subject>Patched-1 Receptor</subject><subject>Patched1</subject><subject>Proteins - metabolism</subject><subject>Receptors, Cell Surface</subject><subject>RNA, Messenger - metabolism</subject><subject>Signal Transduction</subject><subject>Somites - metabolism</subject><subject>Sonic hedgehog</subject><subject>sonic you</subject><subject>Trans-Activators</subject><subject>Transcription Factors - metabolism</subject><subject>u-boot</subject><subject>u-type mutants</subject><subject>you</subject><subject>you-too</subject><subject>Zebrafish</subject><subject>Zebrafish Proteins</subject><subject>Zinc Finger Protein Gli2</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMFLwzAUh4Mobk6vHiUnb515bZo1RxnqhImHTRheQpO-bJGumUkr7L-3Yzt48RQI3_vg9xFyC2wMjImHSjs_BinlWOYgz8gQmMyTXPDVORkyBmkCgokBuYrxizGWFUV2SQbABE8nhRiS1dQ3bfA19Za-ddHUSKdY18lyv0O62KFx1pmydb6hrqHtBukn6lBaFzf0aavD3lO9pzOs1rjxa7pw66asa9esr8mFLeuIN6d3RD6en5bTWTJ_f3mdPs4TkxWsTQrNM4BKp1kqkVepNDlq0FxPcCKFNQVakFxmIjdamokGbvP-JwMOth9RZCNyf_Tugv_uMLZq66LpJ5QN-i4qITn0bt6D4yNogo8xoFW74LZl2Ctg6tBSHVqqQ0t1aNkf3J3Mnd5i9Qc_xuuB4ghgv-_HYVDROGwMVi6gaVXl3X_uX5-Rgto</recordid><startdate>19991215</startdate><enddate>19991215</enddate><creator>Lewis, K.E</creator><creator>Currie, P.D</creator><creator>Roy, S</creator><creator>Schauerte, H</creator><creator>Haffter, P</creator><creator>Ingham, P.W</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19991215</creationdate><title>Control of Muscle Cell-Type Specification in the Zebrafish Embryo by Hedgehog Signalling</title><author>Lewis, K.E ; Currie, P.D ; Roy, S ; Schauerte, H ; Haffter, P ; Ingham, P.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-8b4311db2329e4d29c5eb1b4b7e796fc8ef1949365cb9c7b14f5f193141f06483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Cell Differentiation</topic><topic>chameleon</topic><topic>Fluorescent Antibody Technique</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gli2</topic><topic>Hedgehog Proteins</topic><topic>In Situ Hybridization</topic><topic>Kruppel-Like Transcription Factors</topic><topic>Membrane Proteins - genetics</topic><topic>muscle fibre type</topic><topic>Muscles - embryology</topic><topic>Muscles - metabolism</topic><topic>Mutation</topic><topic>myoD</topic><topic>MyoD Protein - genetics</topic><topic>myosin heavy chain</topic><topic>Myosins - metabolism</topic><topic>Patched Receptors</topic><topic>Patched-1 Receptor</topic><topic>Patched1</topic><topic>Proteins - metabolism</topic><topic>Receptors, Cell Surface</topic><topic>RNA, Messenger - metabolism</topic><topic>Signal Transduction</topic><topic>Somites - metabolism</topic><topic>Sonic hedgehog</topic><topic>sonic you</topic><topic>Trans-Activators</topic><topic>Transcription Factors - metabolism</topic><topic>u-boot</topic><topic>u-type mutants</topic><topic>you</topic><topic>you-too</topic><topic>Zebrafish</topic><topic>Zebrafish Proteins</topic><topic>Zinc Finger Protein Gli2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lewis, K.E</creatorcontrib><creatorcontrib>Currie, P.D</creatorcontrib><creatorcontrib>Roy, S</creatorcontrib><creatorcontrib>Schauerte, H</creatorcontrib><creatorcontrib>Haffter, P</creatorcontrib><creatorcontrib>Ingham, P.W</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lewis, K.E</au><au>Currie, P.D</au><au>Roy, S</au><au>Schauerte, H</au><au>Haffter, P</au><au>Ingham, P.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Control of Muscle Cell-Type Specification in the Zebrafish Embryo by Hedgehog Signalling</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1999-12-15</date><risdate>1999</risdate><volume>216</volume><issue>2</issue><spage>469</spage><epage>480</epage><pages>469-480</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>The specification of different muscle cell types in the zebrafish embryo requires signals that emanate from the axial mesoderm. In previous studies we and others have shown that overexpression of different members of the Hedgehog protein family can induce the differentiation of two types of slow-twitch muscles, the superficially located slow-twitch fibres and the medially located muscle pioneer cells. Here we have investigated the requirement for Hedgehog signalling in the specification of these distinct muscle cell types in two ways: first, by characterising the effects on target gene expression and muscle cell differentiation of the u-type mutants, members of a phenotypic group previously implicated in Hedgehog signalling, and second, by analysing the effects of overexpression of the Patched1 protein, a negative regulator of Hedgehog signalling. 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| ispartof | Developmental biology, 1999-12, Vol.216 (2), p.469-480 |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cell Differentiation chameleon Fluorescent Antibody Technique Gene Expression Regulation, Developmental Gli2 Hedgehog Proteins In Situ Hybridization Kruppel-Like Transcription Factors Membrane Proteins - genetics muscle fibre type Muscles - embryology Muscles - metabolism Mutation myoD MyoD Protein - genetics myosin heavy chain Myosins - metabolism Patched Receptors Patched-1 Receptor Patched1 Proteins - metabolism Receptors, Cell Surface RNA, Messenger - metabolism Signal Transduction Somites - metabolism Sonic hedgehog sonic you Trans-Activators Transcription Factors - metabolism u-boot u-type mutants you you-too Zebrafish Zebrafish Proteins Zinc Finger Protein Gli2 |
| title | Control of Muscle Cell-Type Specification in the Zebrafish Embryo by Hedgehog Signalling |
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